sirt4 in 1  (MedChemExpress)

Journal: Advanced Science

Article Title: Fn14 Controls the SIRT2‐Mediated Deacetylation of Slug to Inhibit the Metastasis of Epithelial Ovarian Cancer

doi: 10.1002/advs.202501552

Figure Lengend Snippet: Fn14 promotes the acetylation‐dependent degradation of Slug protein. A) Upon CHX treatment, western blot analysis detected the expression of Slug in HO8910‐PM cells after infection with Fn14 lentivirus (n = 3). B) Upon CHX treatment, western blot analysis detected the expression of Slug in ES2 cells after transfected with the Fn14 siRNA (n = 3). C) Co‐IP analysis detecting the level of ubiquitination and acetylation of Slug in HO8910‐PM and ES2 cells with specific treatment (n = 3). D) Western blot analysis was performed to detect the expression of Slug in EOC cells with selective inhibitors of Sirt family proteins (Selisistat, SIRT1inhibitor; AGK2, SIRT2 inhibitor; 3‐TYP, SIRT3 inhibitor; SIRT4‐IN‐1, SIRT4 inhibitor; SIRT5 inhibitor 4, SIRT5 inhibitor; OSS_128167, SIRT6 inhibitor; SIRT7 inhibitor 97491, SIRT7 inhibitor). E,F) Western blot analysis was performed to examine the expression of Slug in EOC cells transfected with the SIRT2 siRNA (n = 3). G) Western blot analysis was performed to examine the expression of Slug in EOC cells transfected with SIRT2 ectopically expressing plasmid(n = 3). H)Western blot analysis detecting the level of SIRT2 in HO8910‐PM and ES2 cells with up‐ or down‐regulated expression of Fn14 (n = 3). Unpaired Student's t ‐test was used to compare two groups and one‐way ANOVA was used to compare multiple groups. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Selisistat (Selleck Chemicals), AGK2 (Selleck Chemicals), 3‐TYP (Selleck Chemicals), SIRT4‐IN‐1 (MedChemExpress (MCE), NJ, USA), SIRT4‐IN‐1 (MCE), SIRT5 inhibitor 4 (MCE), OSS_128167 (MCE), SIRT7 inhibitor 97491 (MCE) were used to inhibitSIRT family.

Techniques: Western Blot, Expressing, Infection, Transfection, Co-Immunoprecipitation Assay, Ubiquitin Proteomics, Plasmid Preparation