dimethylcurcumin  (MedChemExpress)

Journal: Endocrinology

Article Title: NADPH Oxidase Mediates Membrane Androgen Receptor–Induced Neurodegeneration

doi: 10.1210/en.2018-01079

Figure Lengend Snippet: Coimmunoprecipitation of AR45, NOX1, and NOX2 to determine the protein complex. NOX1, NOX2, and AR45 proteins were precipitated using specific antibodies and then probed with respective antibodies for NOX1, NOX2, and AR45 to determine protein–protein interactions. NOX1, NOX2, and AR45 interact to form a protein complex. (A) Gαq couples with AR45 but does not interact with either NOX1 or NOX2. (B) Testosterone can bind to the mAR, AR45, and activate multiple signaling pathways via its protein interactions with NOX1, NOX2, and Gαq in a lipid raft. Caveolin (purple), flotillin (blue), and phospholipids (orange) are shown. Co-IP, coimmunoprecipitation; WB, Western blot.

Article Snippet: AR degrader (ASC J9, HY-15194) and NOX2 inhibitor (no. GSK2795039) were purchased from MedChem Express.

Techniques: Co-Immunoprecipitation Assay, Western Blot

Journal: Endocrinology

Article Title: NADPH Oxidase Mediates Membrane Androgen Receptor–Induced Neurodegeneration

doi: 10.1210/en.2018-01079

Figure Lengend Snippet: The effects of selective NOX inhibitors on testosterone-induced cell loss. (A) The selective NOX1 inhibitor, ML171, had no effect on cell viability. H2O2-induced cell loss was not blocked by ML171. ML171 partially blocked testosterone-induced cell loss in the presence of OS. (B) The selective NOX2 inhibitor, GSK2795039, had no effect on cell viability. H2O2-induced cell loss was not blocked by GSK2795039. GSK2795039 partially blocked testosterone-induced cell loss in the presence of OS. Results are reported as mean ± SEM. Results were determined by ANOVA followed by a Fisher least significant difference post hoc test. *P ≤ 0.05 vs all groups; #P ≤ 0.05 vs control; +P ≤ 0.05 vs HT groups. C, vehicle control; H, H2O2; HT, posttreatment T; N, ML 171; N2, GSK2795039; T, 100 nM testosterone, 500 nm DHT-BSA.

Article Snippet: AR degrader (ASC J9, HY-15194) and NOX2 inhibitor (no. GSK2795039) were purchased from MedChem Express.

Techniques: Control

Journal: Endocrinology

Article Title: NADPH Oxidase Mediates Membrane Androgen Receptor–Induced Neurodegeneration

doi: 10.1210/en.2018-01079

Figure Lengend Snippet: The effects of AR degradation on NOX1 and NOX2 protein expression. (A and B) Degrading the AR45, using ASC J9, did not affect NOX1 or NOX2 expression. Data are expressed as a normalized ratio of protein band density of (A) NOX1 and (B) NOX2 against GAPDH and presented as mean ± SD. Results were determined by ANOVA followed by a Fisher least significant difference post hoc test. C, vehicle control; H, H2O2; HT, posttreatment T; J9, ASC J9; T, 100 nM testosterone.

Article Snippet: AR degrader (ASC J9, HY-15194) and NOX2 inhibitor (no. GSK2795039) were purchased from MedChem Express.

Techniques: Expressing, Control

Journal: Endocrinology

Article Title: NADPH Oxidase Mediates Membrane Androgen Receptor–Induced Neurodegeneration

doi: 10.1210/en.2018-01079

Figure Lengend Snippet: Working model. Membrane AR (i.e., AR45) resides in a lipid raft within the plasma membrane and complexes with NOX1, NOX2, and Gαq. Testosterone can bind and activate AR45, which in turn stimulates NOX1 and NOX2, resulting in OS generation. Alternatively, the AR45–NOX complex can upregulate InsP3R activity to increase intracellular calcium release, resulting in increased OS. Dysregulation of this pathway can lead to neurotoxicity, such as during PD. The AR45–Gαq pathway is not involved in testosterone-induced cell loss in OS environments. PLC, Phospholipase C.

Article Snippet: AR degrader (ASC J9, HY-15194) and NOX2 inhibitor (no. GSK2795039) were purchased from MedChem Express.

Techniques: Membrane, Activity Assay