Review



trichostatin a  (MedChemExpress)


Bioz Verified Symbol MedChemExpress is a verified supplier
Bioz Manufacturer Symbol MedChemExpress manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 97
      Buy from Supplier

    Structured Review

    MedChemExpress trichostatin a
    The prometaphase spread technique untethers ecDNA and chromosomes by decompacting mitotic chromatin. A , schematic of an ecDNA-containing cell undergoing mitosis (from left to right : prophase, metaphase, anaphase, telophase). Acentric ecDNA ( red ) have been observed to tether to or “hitchhike” on chromosomes ( blue ) during mitosis to ensure their proper segregation and inheritance by daughter cells. B , prometaphase spreads performed on colcemid-arrested COLO320DM cells with the conventional hypotonic solution (75 mM KCl) and higher osmolarity solutions (100 mM and 125 mM KCl), producing varying amounts of chromosome (DAPI, blue ) individualization and ecDNA ( MYC FISH, red ) untethering. C , quantification of panel B . Left : boxplots quantifying chromosome individualization in prometaphase spreads performed with varying solution osmolarity; from left to right , n = 3, 3, 3 biological replicates and 95, 92, 105 cells; one-way ANOVA, F = 100.7, p < 0.001; ∗∗ p < 0.01 by Tukey’s honestly significant difference (HSD), ns = not significant. Right : quantification of ecDNA untethering; one-way ANOVA, F = 48.3, p < 0.001. Chromosome individualization is represented by the number of connected components identified as chromosomes by ecSeg. ecDNA untethering is represented by the number of ecDNA unattached to chromosomes divided by the total number of ecDNA not completely surrounded by chromosomes. D , prometaphase spreads performed with incubation in 1× PBS using COLO320DM cells pretreated for 8 h with vehicle (0.1% DMSO) or varying concentrations of <t>Trichostatin</t> <t>A</t> (TSA). E , quantification of panel D . Left : quantification of chromosome individualization in prometaphase spreads performed with 1× PBS on cells pretreated with TSA; n = 3, 3, 3, 3, 3, 3 biological replicates and 88, 113, 96, 80, 76, 84 cells; one-way ANOVA, F = 38.5, p < 0.001; ∗∗ p < 0.01 by Tukey’s HSD, ns = not significant. Right : quantification of ecDNA untethering; one-way ANOVA, F = 166.5, p < 0.001. F , linear regression analysis of median chromosome individualization v er s us ecDNA untethering of prometaphase spread conditions in panels B – E (n = 9). G , schematic: electrostatic/hydrophobic mitotic compaction forces at the level of nucleosomes tether ecDNA ( red ) to mitotic chromosomes ( blue ). In panels B–E , scale bar = 10 μm and each data point in graphs represents one cell.
    Trichostatin A, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 97/100, based on 235 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trichostatin a/product/MedChemExpress
    Average 97 stars, based on 235 article reviews
    trichostatin a - by Bioz Stars, 2026-02
    97/100 stars

    Images

    1) Product Images from "Mitotic chromatin compaction tethers extrachromosomal DNA to chromosomes and prevents their mis-segregation into micronuclei"

    Article Title: Mitotic chromatin compaction tethers extrachromosomal DNA to chromosomes and prevents their mis-segregation into micronuclei

    Journal: The Journal of Biological Chemistry

    doi: 10.1016/j.jbc.2025.111081

    The prometaphase spread technique untethers ecDNA and chromosomes by decompacting mitotic chromatin. A , schematic of an ecDNA-containing cell undergoing mitosis (from left to right : prophase, metaphase, anaphase, telophase). Acentric ecDNA ( red ) have been observed to tether to or “hitchhike” on chromosomes ( blue ) during mitosis to ensure their proper segregation and inheritance by daughter cells. B , prometaphase spreads performed on colcemid-arrested COLO320DM cells with the conventional hypotonic solution (75 mM KCl) and higher osmolarity solutions (100 mM and 125 mM KCl), producing varying amounts of chromosome (DAPI, blue ) individualization and ecDNA ( MYC FISH, red ) untethering. C , quantification of panel B . Left : boxplots quantifying chromosome individualization in prometaphase spreads performed with varying solution osmolarity; from left to right , n = 3, 3, 3 biological replicates and 95, 92, 105 cells; one-way ANOVA, F = 100.7, p < 0.001; ∗∗ p < 0.01 by Tukey’s honestly significant difference (HSD), ns = not significant. Right : quantification of ecDNA untethering; one-way ANOVA, F = 48.3, p < 0.001. Chromosome individualization is represented by the number of connected components identified as chromosomes by ecSeg. ecDNA untethering is represented by the number of ecDNA unattached to chromosomes divided by the total number of ecDNA not completely surrounded by chromosomes. D , prometaphase spreads performed with incubation in 1× PBS using COLO320DM cells pretreated for 8 h with vehicle (0.1% DMSO) or varying concentrations of Trichostatin A (TSA). E , quantification of panel D . Left : quantification of chromosome individualization in prometaphase spreads performed with 1× PBS on cells pretreated with TSA; n = 3, 3, 3, 3, 3, 3 biological replicates and 88, 113, 96, 80, 76, 84 cells; one-way ANOVA, F = 38.5, p < 0.001; ∗∗ p < 0.01 by Tukey’s HSD, ns = not significant. Right : quantification of ecDNA untethering; one-way ANOVA, F = 166.5, p < 0.001. F , linear regression analysis of median chromosome individualization v er s us ecDNA untethering of prometaphase spread conditions in panels B – E (n = 9). G , schematic: electrostatic/hydrophobic mitotic compaction forces at the level of nucleosomes tether ecDNA ( red ) to mitotic chromosomes ( blue ). In panels B–E , scale bar = 10 μm and each data point in graphs represents one cell.
    Figure Legend Snippet: The prometaphase spread technique untethers ecDNA and chromosomes by decompacting mitotic chromatin. A , schematic of an ecDNA-containing cell undergoing mitosis (from left to right : prophase, metaphase, anaphase, telophase). Acentric ecDNA ( red ) have been observed to tether to or “hitchhike” on chromosomes ( blue ) during mitosis to ensure their proper segregation and inheritance by daughter cells. B , prometaphase spreads performed on colcemid-arrested COLO320DM cells with the conventional hypotonic solution (75 mM KCl) and higher osmolarity solutions (100 mM and 125 mM KCl), producing varying amounts of chromosome (DAPI, blue ) individualization and ecDNA ( MYC FISH, red ) untethering. C , quantification of panel B . Left : boxplots quantifying chromosome individualization in prometaphase spreads performed with varying solution osmolarity; from left to right , n = 3, 3, 3 biological replicates and 95, 92, 105 cells; one-way ANOVA, F = 100.7, p < 0.001; ∗∗ p < 0.01 by Tukey’s honestly significant difference (HSD), ns = not significant. Right : quantification of ecDNA untethering; one-way ANOVA, F = 48.3, p < 0.001. Chromosome individualization is represented by the number of connected components identified as chromosomes by ecSeg. ecDNA untethering is represented by the number of ecDNA unattached to chromosomes divided by the total number of ecDNA not completely surrounded by chromosomes. D , prometaphase spreads performed with incubation in 1× PBS using COLO320DM cells pretreated for 8 h with vehicle (0.1% DMSO) or varying concentrations of Trichostatin A (TSA). E , quantification of panel D . Left : quantification of chromosome individualization in prometaphase spreads performed with 1× PBS on cells pretreated with TSA; n = 3, 3, 3, 3, 3, 3 biological replicates and 88, 113, 96, 80, 76, 84 cells; one-way ANOVA, F = 38.5, p < 0.001; ∗∗ p < 0.01 by Tukey’s HSD, ns = not significant. Right : quantification of ecDNA untethering; one-way ANOVA, F = 166.5, p < 0.001. F , linear regression analysis of median chromosome individualization v er s us ecDNA untethering of prometaphase spread conditions in panels B – E (n = 9). G , schematic: electrostatic/hydrophobic mitotic compaction forces at the level of nucleosomes tether ecDNA ( red ) to mitotic chromosomes ( blue ). In panels B–E , scale bar = 10 μm and each data point in graphs represents one cell.

    Techniques Used: Incubation



    Similar Products

    trichostatin a  (MedChemExpress)
    97
    MedChemExpress trichostatin a
    The prometaphase spread technique untethers ecDNA and chromosomes by decompacting mitotic chromatin. A , schematic of an ecDNA-containing cell undergoing mitosis (from left to right : prophase, metaphase, anaphase, telophase). Acentric ecDNA ( red ) have been observed to tether to or “hitchhike” on chromosomes ( blue ) during mitosis to ensure their proper segregation and inheritance by daughter cells. B , prometaphase spreads performed on colcemid-arrested COLO320DM cells with the conventional hypotonic solution (75 mM KCl) and higher osmolarity solutions (100 mM and 125 mM KCl), producing varying amounts of chromosome (DAPI, blue ) individualization and ecDNA ( MYC FISH, red ) untethering. C , quantification of panel B . Left : boxplots quantifying chromosome individualization in prometaphase spreads performed with varying solution osmolarity; from left to right , n = 3, 3, 3 biological replicates and 95, 92, 105 cells; one-way ANOVA, F = 100.7, p < 0.001; ∗∗ p < 0.01 by Tukey’s honestly significant difference (HSD), ns = not significant. Right : quantification of ecDNA untethering; one-way ANOVA, F = 48.3, p < 0.001. Chromosome individualization is represented by the number of connected components identified as chromosomes by ecSeg. ecDNA untethering is represented by the number of ecDNA unattached to chromosomes divided by the total number of ecDNA not completely surrounded by chromosomes. D , prometaphase spreads performed with incubation in 1× PBS using COLO320DM cells pretreated for 8 h with vehicle (0.1% DMSO) or varying concentrations of <t>Trichostatin</t> <t>A</t> (TSA). E , quantification of panel D . Left : quantification of chromosome individualization in prometaphase spreads performed with 1× PBS on cells pretreated with TSA; n = 3, 3, 3, 3, 3, 3 biological replicates and 88, 113, 96, 80, 76, 84 cells; one-way ANOVA, F = 38.5, p < 0.001; ∗∗ p < 0.01 by Tukey’s HSD, ns = not significant. Right : quantification of ecDNA untethering; one-way ANOVA, F = 166.5, p < 0.001. F , linear regression analysis of median chromosome individualization v er s us ecDNA untethering of prometaphase spread conditions in panels B – E (n = 9). G , schematic: electrostatic/hydrophobic mitotic compaction forces at the level of nucleosomes tether ecDNA ( red ) to mitotic chromosomes ( blue ). In panels B–E , scale bar = 10 μm and each data point in graphs represents one cell.
    Trichostatin A, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trichostatin a/product/MedChemExpress
    Average 97 stars, based on 1 article reviews
    trichostatin a - by Bioz Stars, 2026-02
    97/100 stars
    		  Buy from Supplier
    tsa  (MedChemExpress)
    97
    MedChemExpress tsa
    The prometaphase spread technique untethers ecDNA and chromosomes by decompacting mitotic chromatin. A , schematic of an ecDNA-containing cell undergoing mitosis (from left to right : prophase, metaphase, anaphase, telophase). Acentric ecDNA ( red ) have been observed to tether to or “hitchhike” on chromosomes ( blue ) during mitosis to ensure their proper segregation and inheritance by daughter cells. B , prometaphase spreads performed on colcemid-arrested COLO320DM cells with the conventional hypotonic solution (75 mM KCl) and higher osmolarity solutions (100 mM and 125 mM KCl), producing varying amounts of chromosome (DAPI, blue ) individualization and ecDNA ( MYC FISH, red ) untethering. C , quantification of panel B . Left : boxplots quantifying chromosome individualization in prometaphase spreads performed with varying solution osmolarity; from left to right , n = 3, 3, 3 biological replicates and 95, 92, 105 cells; one-way ANOVA, F = 100.7, p < 0.001; ∗∗ p < 0.01 by Tukey’s honestly significant difference (HSD), ns = not significant. Right : quantification of ecDNA untethering; one-way ANOVA, F = 48.3, p < 0.001. Chromosome individualization is represented by the number of connected components identified as chromosomes by ecSeg. ecDNA untethering is represented by the number of ecDNA unattached to chromosomes divided by the total number of ecDNA not completely surrounded by chromosomes. D , prometaphase spreads performed with incubation in 1× PBS using COLO320DM cells pretreated for 8 h with vehicle (0.1% DMSO) or varying concentrations of <t>Trichostatin</t> <t>A</t> (TSA). E , quantification of panel D . Left : quantification of chromosome individualization in prometaphase spreads performed with 1× PBS on cells pretreated with TSA; n = 3, 3, 3, 3, 3, 3 biological replicates and 88, 113, 96, 80, 76, 84 cells; one-way ANOVA, F = 38.5, p < 0.001; ∗∗ p < 0.01 by Tukey’s HSD, ns = not significant. Right : quantification of ecDNA untethering; one-way ANOVA, F = 166.5, p < 0.001. F , linear regression analysis of median chromosome individualization v er s us ecDNA untethering of prometaphase spread conditions in panels B – E (n = 9). G , schematic: electrostatic/hydrophobic mitotic compaction forces at the level of nucleosomes tether ecDNA ( red ) to mitotic chromosomes ( blue ). In panels B–E , scale bar = 10 μm and each data point in graphs represents one cell.
    Tsa, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tsa/product/MedChemExpress
    Average 97 stars, based on 1 article reviews
    tsa - by Bioz Stars, 2026-02
    97/100 stars
    		  Buy from Supplier
    hy 15144 mdl 12330a medchemexpress  (MedChemExpress)
    97
    MedChemExpress hy 15144 mdl 12330a medchemexpress
    The prometaphase spread technique untethers ecDNA and chromosomes by decompacting mitotic chromatin. A , schematic of an ecDNA-containing cell undergoing mitosis (from left to right : prophase, metaphase, anaphase, telophase). Acentric ecDNA ( red ) have been observed to tether to or “hitchhike” on chromosomes ( blue ) during mitosis to ensure their proper segregation and inheritance by daughter cells. B , prometaphase spreads performed on colcemid-arrested COLO320DM cells with the conventional hypotonic solution (75 mM KCl) and higher osmolarity solutions (100 mM and 125 mM KCl), producing varying amounts of chromosome (DAPI, blue ) individualization and ecDNA ( MYC FISH, red ) untethering. C , quantification of panel B . Left : boxplots quantifying chromosome individualization in prometaphase spreads performed with varying solution osmolarity; from left to right , n = 3, 3, 3 biological replicates and 95, 92, 105 cells; one-way ANOVA, F = 100.7, p < 0.001; ∗∗ p < 0.01 by Tukey’s honestly significant difference (HSD), ns = not significant. Right : quantification of ecDNA untethering; one-way ANOVA, F = 48.3, p < 0.001. Chromosome individualization is represented by the number of connected components identified as chromosomes by ecSeg. ecDNA untethering is represented by the number of ecDNA unattached to chromosomes divided by the total number of ecDNA not completely surrounded by chromosomes. D , prometaphase spreads performed with incubation in 1× PBS using COLO320DM cells pretreated for 8 h with vehicle (0.1% DMSO) or varying concentrations of <t>Trichostatin</t> <t>A</t> (TSA). E , quantification of panel D . Left : quantification of chromosome individualization in prometaphase spreads performed with 1× PBS on cells pretreated with TSA; n = 3, 3, 3, 3, 3, 3 biological replicates and 88, 113, 96, 80, 76, 84 cells; one-way ANOVA, F = 38.5, p < 0.001; ∗∗ p < 0.01 by Tukey’s HSD, ns = not significant. Right : quantification of ecDNA untethering; one-way ANOVA, F = 166.5, p < 0.001. F , linear regression analysis of median chromosome individualization v er s us ecDNA untethering of prometaphase spread conditions in panels B – E (n = 9). G , schematic: electrostatic/hydrophobic mitotic compaction forces at the level of nucleosomes tether ecDNA ( red ) to mitotic chromosomes ( blue ). In panels B–E , scale bar = 10 μm and each data point in graphs represents one cell.
    Hy 15144 Mdl 12330a Medchemexpress, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hy 15144 mdl 12330a medchemexpress/product/MedChemExpress
    Average 97 stars, based on 1 article reviews
    hy 15144 mdl 12330a medchemexpress - by Bioz Stars, 2026-02
    97/100 stars
    		  Buy from Supplier

    Image Search Results


    The prometaphase spread technique untethers ecDNA and chromosomes by decompacting mitotic chromatin. A , schematic of an ecDNA-containing cell undergoing mitosis (from left to right : prophase, metaphase, anaphase, telophase). Acentric ecDNA ( red ) have been observed to tether to or “hitchhike” on chromosomes ( blue ) during mitosis to ensure their proper segregation and inheritance by daughter cells. B , prometaphase spreads performed on colcemid-arrested COLO320DM cells with the conventional hypotonic solution (75 mM KCl) and higher osmolarity solutions (100 mM and 125 mM KCl), producing varying amounts of chromosome (DAPI, blue ) individualization and ecDNA ( MYC FISH, red ) untethering. C , quantification of panel B . Left : boxplots quantifying chromosome individualization in prometaphase spreads performed with varying solution osmolarity; from left to right , n = 3, 3, 3 biological replicates and 95, 92, 105 cells; one-way ANOVA, F = 100.7, p < 0.001; ∗∗ p < 0.01 by Tukey’s honestly significant difference (HSD), ns = not significant. Right : quantification of ecDNA untethering; one-way ANOVA, F = 48.3, p < 0.001. Chromosome individualization is represented by the number of connected components identified as chromosomes by ecSeg. ecDNA untethering is represented by the number of ecDNA unattached to chromosomes divided by the total number of ecDNA not completely surrounded by chromosomes. D , prometaphase spreads performed with incubation in 1× PBS using COLO320DM cells pretreated for 8 h with vehicle (0.1% DMSO) or varying concentrations of Trichostatin A (TSA). E , quantification of panel D . Left : quantification of chromosome individualization in prometaphase spreads performed with 1× PBS on cells pretreated with TSA; n = 3, 3, 3, 3, 3, 3 biological replicates and 88, 113, 96, 80, 76, 84 cells; one-way ANOVA, F = 38.5, p < 0.001; ∗∗ p < 0.01 by Tukey’s HSD, ns = not significant. Right : quantification of ecDNA untethering; one-way ANOVA, F = 166.5, p < 0.001. F , linear regression analysis of median chromosome individualization v er s us ecDNA untethering of prometaphase spread conditions in panels B – E (n = 9). G , schematic: electrostatic/hydrophobic mitotic compaction forces at the level of nucleosomes tether ecDNA ( red ) to mitotic chromosomes ( blue ). In panels B–E , scale bar = 10 μm and each data point in graphs represents one cell.

    Journal: The Journal of Biological Chemistry

    Article Title: Mitotic chromatin compaction tethers extrachromosomal DNA to chromosomes and prevents their mis-segregation into micronuclei

    doi: 10.1016/j.jbc.2025.111081

    Figure Lengend Snippet: The prometaphase spread technique untethers ecDNA and chromosomes by decompacting mitotic chromatin. A , schematic of an ecDNA-containing cell undergoing mitosis (from left to right : prophase, metaphase, anaphase, telophase). Acentric ecDNA ( red ) have been observed to tether to or “hitchhike” on chromosomes ( blue ) during mitosis to ensure their proper segregation and inheritance by daughter cells. B , prometaphase spreads performed on colcemid-arrested COLO320DM cells with the conventional hypotonic solution (75 mM KCl) and higher osmolarity solutions (100 mM and 125 mM KCl), producing varying amounts of chromosome (DAPI, blue ) individualization and ecDNA ( MYC FISH, red ) untethering. C , quantification of panel B . Left : boxplots quantifying chromosome individualization in prometaphase spreads performed with varying solution osmolarity; from left to right , n = 3, 3, 3 biological replicates and 95, 92, 105 cells; one-way ANOVA, F = 100.7, p < 0.001; ∗∗ p < 0.01 by Tukey’s honestly significant difference (HSD), ns = not significant. Right : quantification of ecDNA untethering; one-way ANOVA, F = 48.3, p < 0.001. Chromosome individualization is represented by the number of connected components identified as chromosomes by ecSeg. ecDNA untethering is represented by the number of ecDNA unattached to chromosomes divided by the total number of ecDNA not completely surrounded by chromosomes. D , prometaphase spreads performed with incubation in 1× PBS using COLO320DM cells pretreated for 8 h with vehicle (0.1% DMSO) or varying concentrations of Trichostatin A (TSA). E , quantification of panel D . Left : quantification of chromosome individualization in prometaphase spreads performed with 1× PBS on cells pretreated with TSA; n = 3, 3, 3, 3, 3, 3 biological replicates and 88, 113, 96, 80, 76, 84 cells; one-way ANOVA, F = 38.5, p < 0.001; ∗∗ p < 0.01 by Tukey’s HSD, ns = not significant. Right : quantification of ecDNA untethering; one-way ANOVA, F = 166.5, p < 0.001. F , linear regression analysis of median chromosome individualization v er s us ecDNA untethering of prometaphase spread conditions in panels B – E (n = 9). G , schematic: electrostatic/hydrophobic mitotic compaction forces at the level of nucleosomes tether ecDNA ( red ) to mitotic chromosomes ( blue ). In panels B–E , scale bar = 10 μm and each data point in graphs represents one cell.

    Article Snippet: The following chemicals were used in the study: MG132 (Selleck S2619), Colcemid (Gibco 15,210–040), Trichostatin A (MedChemExpress HY-15144), Belinostat (MedChemExpress HY-10225), Abexinostat (PCI-24781, MedChemExpress HY-10990), Fimepinostat (CUDC-907, MedChemExpress HY-13522), Pracinostat (SB939, MedChemExpress HY-13322), LMK235 (MedChemExpress HY-18998), TMP269 (MedChemExpress HY-18360), Mocetinostat (MGCD0103, MedChemExpress HY-12164), Entinostat (MS275, Tocris 6208), RGFP966 (Cayman 16917), Santacruzamate A (CAY10683, Cayman 15,403), Tasquinimod (MedChemExpress HY-10528), BRD73954 (MedChemExpress HY-18700), Nicotinamide (Cayman 11127), EX527 (Cayman 10009798), CTPB (Cayman 19570), 5-Azacytidine (Sigma Aldrich A2385), GSK343 (Selleck S7164), RRx001 (Selleck S8405), ZM447439 (Selleck S1103), Hesperadin (Selleck S1529), Paprotrain (Selleck E0779), and DAPI (Invitrogen D1306).

    Techniques: Incubation