vildagliptin  (MedChemExpress)

Journal: bioRxiv

Article Title: Biochemical characterization of a Mycobacterium tuberculosis prolyl dipeptidyl peptidase that displays a remarkable thermostability

doi: 10.1101/2022.07.09.499413

Figure Lengend Snippet: hDPP4 inhibitors bind to MtDPP and inhibit its activity. (A) ΔT m of MtDPP after treatment with hDPP4 inhibitors. (B) MtDPP IC 50 inhibition assays with hDPP4 inhibitors depicting inhibition by compounds SYR-322, BI-1356, MK0431 and LAF237. In both, error bars depict standard deviation derived from three experiments in triplicates. TSA melt curve plots can be found in Figure S6D.

Article Snippet: hDPP4 inhibitors SYR-322 (MedChemExpress) , BI-1356 (Macklin) , MK0431 (Aladdin) ( ) and LAF237 (Aladdin) ( ) (Fig. S5) were added separately into enzymatic assays at concentrations ranging from 1×10 −6 to 1 mM and incubated with purified MtDPP in aforementioned enzymatic assay conditions at RT for 15 min prior to the addition of an h-Gly-Pro-βNA substrate.

Techniques: Activity Assay, Inhibition, Standard Deviation, Derivative Assay