hy 141540 hepes beyotime  (MedChemExpress)

Journal: Cell Death & Disease

Article Title: HAT1 functions as a lactyltransferase and mediates RPA1 lactylation to promote DNA repair and radioresistance in lung adenocarcinoma

doi: 10.1038/s41419-025-08113-x

Figure Lengend Snippet: A Survival map analysis using the GEPIA database with a median cutoff. ACC (Adrenocortical carcinoma), BLCA (Bladder Urothelial Carcinoma), BRCA (Breast invasive carcinoma), CESC (Cervical squamous cell carcinoma and endocervical adenocarcinoma), CHOL(Cholangio carcinoma), COAD (Colon adenocarcinoma), DLBC (Lymphoid Neoplasm Diffuse Large B-cell Lymphoma), ESCA (Esophageal carcinoma), GBM (Glioblastoma multiforme), HNSC (Head and Neck squamous cell carcinoma), KICH (Kidney Chromophobe), KIRC (Kidney renal clear cell carcinoma), KIRP (Kidney renal papillary cell carcinoma), LAML (Acute Myeloid Leukemia), LGG(Brain Lower Grade Glioma), LIHC (Liver hepatocellular carcinoma), LUAD (Lung adenocarcinoma), LUSC (Lung squamous cell carcinoma), MESO (Mesothelioma), OV (Ovarian serous cystadenocarcinoma), PAAD (Pancreatic adenocarcinoma), PCPG (Pheochromocytoma and Paraganglioma), PRAD (Prostate adenocarcinoma), READ (Rectum adenocarcinoma), SARC (Sarcoma), SKCM (Skin Cutaneous Melanoma), STAD (Stomach adenocarcinoma), TGCT (Testicular Germ Cell Tumors), THCA (Thyroid carcinoma), THYM (Thymoma), UCEC (Uterine Corpus Endometrial Carcinoma), UCS (Uterine Carcinosarcoma), UVM (Uveal Melanoma). B Kaplan-Meier analysis of patients with high or low expression of HAT1 using the Tumor Lung Adenocarcinoma data set. C Kaplan-Meier analysis of patients with high or low expression of HAT1 using Mixed Lung Adenocarcinoma data set. D Analysis of the expression of HAT1 in LUAD GEPIA samples. E Analysis of the expression of HAT1 in LUAD CPTAC samples. F HAT1 IHC staining in normal tissues and in tumor tissues of different grades, including Grade Ⅰ, Grade Ⅱ, Grade Ⅲ and Grade Ⅳ (scale bar = 10 μm). G Analysis of the expression of HAT1 in normal tissues and in tumor tissues of different grades including Grade Ⅰ, Grade Ⅱ and Grade Ⅲ using LUAD CPTAC data set. H The expression of HAT1 in 14 pairs of normal and tumor tissues. ( I ) Relative protein expression of HAT1 in panel H. Data were analyzed using two-way analysis of variance (ANOVA), ** P < 0.01.

Article Snippet: Lactyl-CoA (#HY-141540) and HAT1 inhibitor JG-2016 (#HY-154944) were purchased from MedChemExpress (MCE).

Techniques: Expressing, Immunohistochemistry

Journal: Cell Death & Disease

Article Title: HAT1 functions as a lactyltransferase and mediates RPA1 lactylation to promote DNA repair and radioresistance in lung adenocarcinoma

doi: 10.1038/s41419-025-08113-x

Figure Lengend Snippet: A The level of pan L-Lactyl Lysine (Pan-Kla) in HAT1-KO Cells. B The expression of pan Crotonyllysine (Pan-Kcr) in HAT1-KO Cells. C The level of pan Butyryllysine (Pan-Kbu) in HAT1-KO Cells. D The expression of global Succinyllysine (Pan-Ksu) in HAT1-KO Cells. E The level of global Glutaryllysine (Pan-Kglu) in HAT1-KO Cells. F The expression of global Benzoyllysine (Pan-Kbz) in HAT1-KO Cells. G The level of pan Propionyllysine (Pan-Kpr) in HAT1-KO Cells. H The expression of global β-Hydroxybutyryllysine (Pan-Kbhb) in HAT1-KO Cells. I The level of global Malonyllysine (Pan-Kma) in HAT1-KO Cells. J The level of pan 2-Hydroxyisobutyryllysine (Pan-Khib) in HAT1-KO Cells. K The expression of global Acetyllysine (Pan-Kac) in HAT1-KO Cells. L Coomassie brilliant blue staining of total proteins in control cells and in HAT1-KO cells. M The expression of pan-Kla, HAT1 and GAPDH in different monoclonal cells depleting of HAT1. N NALA induced pan-Kla in HAT1-KO cells. O Coomassie brilliant blue staining of total proteins induced by NALA in HAT1-KO cells.

Article Snippet: Lactyl-CoA (#HY-141540) and HAT1 inhibitor JG-2016 (#HY-154944) were purchased from MedChemExpress (MCE).

Techniques: Expressing, Staining, Control

Journal: Cell Death & Disease

Article Title: HAT1 functions as a lactyltransferase and mediates RPA1 lactylation to promote DNA repair and radioresistance in lung adenocarcinoma

doi: 10.1038/s41419-025-08113-x

Figure Lengend Snippet: A The IR-induced foci of γ-H2AX in A549 cells abolishing of HAT1 (scale bar = 5 μm; n = 25). B The IR-induced foci of γ-H2AX in H1299 cells depleting of HAT1 (scale bar = 5 μm; n = 25). C The IR-induced tailed DNA in HAT1-KO A549 cells (scale bar=10 μm; n = 50). D The tailed DNA in H1299 cells depleting of HAT1 (scale bar = 10 μm; n = 50). E The expression of γ-H2AX, HAT1 and β-actin in monoclonal A549 cells depleting of HAT1 (clone #3 and clone #14). F The expression of γ-H2AX, HAT1 and β-actin in monoclonal H1299 cells depleting of HAT1 (clone #19 and clone #26). G The clonogenic ability of A549 cells treated with IR. H The colony-forming ability of H1299 cells treated with IR. All data were analyzed using two-way analysis of variance (ANOVA), ** P < 0.01.

Article Snippet: Lactyl-CoA (#HY-141540) and HAT1 inhibitor JG-2016 (#HY-154944) were purchased from MedChemExpress (MCE).

Techniques: Expressing

Journal: Cell Death & Disease

Article Title: HAT1 functions as a lactyltransferase and mediates RPA1 lactylation to promote DNA repair and radioresistance in lung adenocarcinoma

doi: 10.1038/s41419-025-08113-x

Figure Lengend Snippet: A Schematic diagram of HR reporter systems. B GFP-positive cells determined by HR reporter systems using flow cytometry. C Relative HR efficiency of control LUAD cells and HAT1-KO LUAD cells. D Schematic diagram of NHEJ reporter systems. E GFP-positive cells determined by NHEJ reporter systems using flow cytometry. F Relative NHEJ efficiency of control LUAD cells and HAT1-KO LUAD cells. G The IR-induced foci of RPA1 in A549 cells, abolishing of HAT1 (scale bar = 5 μm; n = 25). H The IR-induced foci of RPA1 in H1299 cells depleted of HAT1 (scale bar = 5 μm; n = 25). I The IR-induced foci of RAD51in A549 cells, abolishing of HAT1 (scale bar = 5 μm; n = 25). J The IR-induced foci of RAD51 in H1299 cells depleting of HAT1 (scale bar = 5 μm; n = 25). All data were analyzed using two-way analysis of variance (ANOVA), ** P < 0.01.

Article Snippet: Lactyl-CoA (#HY-141540) and HAT1 inhibitor JG-2016 (#HY-154944) were purchased from MedChemExpress (MCE).

Techniques: Flow Cytometry, Control

Journal: Cell Death & Disease

Article Title: HAT1 functions as a lactyltransferase and mediates RPA1 lactylation to promote DNA repair and radioresistance in lung adenocarcinoma

doi: 10.1038/s41419-025-08113-x

Figure Lengend Snippet: A Intersection among lysine-lactylated substrates, HAT1-interacted proteins and the gene set of 28 HR proteins. B Secondary mass spectrometry map of RPA1 K88 lactylation modification. C Sliver staining of proteins interacted with HAT1. D Interaction of HAT1 with RPA1 in A549 cells. E Interaction of HAT1 with RPA1 in H1299 cells. F Interaction of exogenous HAT1 with exogenous RPA1 in 293 T cells detected by Flag-Trap. G Interaction of exogenous HAT1 with exogenous RPA1 in 293 T cells detected by HA-Trap. H Validation of RPA1 lactylation in A549 cells. I Confirmation of RPA1 lactylation in H1299 cells. J Detection of lactylated RPA1 in A549 cells depleting of HAT1. K Detection of lactylated RPA1 in H1299 cells depleting of HAT1. L Detection of lactylated RPA1 in HEK-293T cells overexpressed with 7 acetyltransferase of KAT family including P300, CBP, HAT1, KAT2A, KAT2B, KAT5 and KAT8. M Schematic diagram of molecular docking between HAT1 and La-CoA. N In vitro lactylation assay showing lysine lactyltransferase activity of HAT1 towards RPA1.

Article Snippet: Lactyl-CoA (#HY-141540) and HAT1 inhibitor JG-2016 (#HY-154944) were purchased from MedChemExpress (MCE).

Techniques: Mass Spectrometry, Modification, Staining, Biomarker Discovery, In Vitro, Activity Assay

Journal: Cell Death & Disease

Article Title: HAT1 functions as a lactyltransferase and mediates RPA1 lactylation to promote DNA repair and radioresistance in lung adenocarcinoma

doi: 10.1038/s41419-025-08113-x

Figure Lengend Snippet: A Detection of HA-Trap binded acetyltransferase in cells overexpressed 8 acetyltransferase of KAT family, including P300, CBP, HAT1, KAT2A, KAT2B, KAT5, KAT7 and KAT8. B The level of lactylated acetyltransferases in cells overexpressed 8 acetyltransferase of KAT family, including P300, CBP, HAT1, KAT2A, KAT2B, KAT5, KAT7, and KAT8 (the red arrows represent the corresponding lactylated proteins). C The expression of acetyltransferase in whole cell lysates overexpressed 8 acetyltransferase of KAT family, including P300, CBP, HAT1, KAT2A, KAT2B, KAT5, KAT7, and KAT8. D Secondary mass spectrometry map of HAT5 K15 lactylation modification. E Sequence alignment of the K15 site of HAT1 in diverse species. F The level of NALA-induced lactylated HAT1 in 293 T cells. G The expression of lactylated HAT1 in WT cells as well as in mutant cells with HAT1 K15R. H The level of lactylated RPA1 in HAT1-WT cells as well as in mutant cells with HAT1-K15R. I Relative HR efficiency in WT cells and in mutant cells with HAT1-K15R. J The IR-induced foci of RPA1 in A549 cells expressing HAT1-WT proteins or mutant HAT1-K15R (scale bar=5 μm; n = 25). K The colony-forming ability of A549 cells expressing HAT1-WT proteins or mutant HAT1-K15R. All data were analyzed using two-way analysis of variance (ANOVA), ** P < 0.01.

Article Snippet: Lactyl-CoA (#HY-141540) and HAT1 inhibitor JG-2016 (#HY-154944) were purchased from MedChemExpress (MCE).

Techniques: Expressing, Mass Spectrometry, Modification, Sequencing, Mutagenesis

Journal: Cell Death & Disease

Article Title: HAT1 functions as a lactyltransferase and mediates RPA1 lactylation to promote DNA repair and radioresistance in lung adenocarcinoma

doi: 10.1038/s41419-025-08113-x

Figure Lengend Snippet: A Photograph of tumors from indicated mice, including the control group, HAT1-KO group, and HAT1 inhibitor group with or without IR. B The tumor volume of the indicated mice, including the control group, the HAT1-KO group, and the HAT1 inhibitor group with or without IR. C The tumor weight of indicated mice, including the control group, HAT1-KO group, and HAT1 inhibitor group with or without IR. D H&E staining and IHC staining with Pan-Kla, HAT1, and Ki-67 in tumors from the control group, HAT1-KO group, and HAT1 inhibitor group with or without IR (scale bar = 30 μm). E The expression of global lactylated proteins, HAT1, and GAPDH in 14 pairs of normal tissues and tumor samples. F IHC staining with HAT1 and Pan-Kla on tissue microarray of consecutive sections (scale bar=20 μm). G The analysis of correlation between HAT1 and Pan-Kla according to intensity from panel E. H The analysis of correlation between HAT1 and Pan-Kla according to IHC score from F . All data were analyzed using two-way analysis of variance (ANOVA), * P < 0.05, ** P < 0.01.

Article Snippet: Lactyl-CoA (#HY-141540) and HAT1 inhibitor JG-2016 (#HY-154944) were purchased from MedChemExpress (MCE).

Techniques: Control, Staining, Immunohistochemistry, Expressing, Microarray