sn38  (MedChemExpress)

Journal: Polymers

Article Title: Correlation of Polymer–drug Composition with Micelle Properties, Performance, and Cytotoxicity for the Oligoelectrolyte-mediated pH-triggered Release of Hydrophobic Drugs

doi: 10.3390/polym18020247

Figure Lengend Snippet: pH-dependent drug release from PEG 10 PDL 10 micelles. Rows correspond to the respective drugs indicated on the left-hand side. ( a ) In vitro dialysis assay showing the cumulative release of drugs (all 0.1 mg/mg of copolymer) from free drug solutions and micelles with various OVP loadings (0, 0.1, 0.2, and 0.3 mg/mg of copolymer) against PBS (pH 7.4) for several hours (−2 to 0 h), followed by acetate buffer (pH 4.5) for 7 h (0 to 7 h); experiments were conducted at 22 ± 1 °C and the drug release was measured via UV-vis spectrophotometry (DOX, GP and SN38) or high-performance liquid chromatography (HPLC) (PX). Data are shown as the average cumulative release (%) + SD ( n = 3). Only symbols represent the experimental data; the dashed lines are guides to the eyes. Release of the drugs at pH 4.5 after 2 and 7 h (excluding any release at pH 7) was analysed with respect to the OVP loading (0, 0.1, 0.2 and 0.3 mg/mg of copolymer) as ( b ) the mole amount of drug released, ( c ) the percentage increase in drug release relative to micelles without OVP, and ( d ) the amount of drug released relative to the amount of OVP loaded (nmol/nmol).

Article Snippet: DOX (≥98%), PX (≥99%), SN38 (≥99.8%) and GP (≥98%) were purchased from the Cayman Chemical Company (Ann Arbor, MI, USA), Selleck Chemicals (Houston, TX, USA), MedChemExpress (Monmouth Junction, NJ, USA) and Shaanxi Ciyuan biotech (Xi’an, China), respectively.

Techniques: In Vitro, Spectrophotometry, High Performance Liquid Chromatography

Journal: Polymers

Article Title: Correlation of Polymer–drug Composition with Micelle Properties, Performance, and Cytotoxicity for the Oligoelectrolyte-mediated pH-triggered Release of Hydrophobic Drugs

doi: 10.3390/polym18020247

Figure Lengend Snippet: Fold change in reduction in metabolic activity as a function of micelle concentration for drug-loaded PEG 14 PS 12 OVP z micelles relative to drug-loaded PEG 14 PS 12 micelles (no OVP), following incubation for 48 h; drug concentrations (3.13-50.0 µg/mL) are 10 times less than micelle concentrations. Fold change for ( a ) PEG 14 PS 12 OVP 0.3 SN38 0.1 and PEG 14 PS 12 OVP 0.3 PX 0.1 micelles with SKOV-3 cells, ( b ) PEG 14 PS 12 OVP 0.1 DOX 0.1 and PEG 14 PS 12 OVP 0.3 DOX 0.1 micelles with ES-2 ( left ) and PC-3 ( right ) cells and ( c ) PEG 14 PS 12 OVP 0.1 SN38 0.1 and PEG 14 PS 12 OVP 0.3 SN38 0.1 micelles with ES-2 ( left ) and PC-3 ( right ) cells.

Article Snippet: DOX (≥98%), PX (≥99%), SN38 (≥99.8%) and GP (≥98%) were purchased from the Cayman Chemical Company (Ann Arbor, MI, USA), Selleck Chemicals (Houston, TX, USA), MedChemExpress (Monmouth Junction, NJ, USA) and Shaanxi Ciyuan biotech (Xi’an, China), respectively.

Techniques: Activity Assay, Concentration Assay, Incubation