Journal: Acta Pharmaceutica Sinica. B
Article Title: A novel C-3-substituted oleanolic acid benzyl amide derivative exhibits therapeutic potential against influenza A by targeting PA–PB1 interactions and modulating host macrophage inflammation
doi: 10.1016/j.apsb.2025.05.031
Figure Lengend Snippet: High resistance barrier of compound A5 in vitro . (A) Is a diagrammatic drawing representing the serial viral passage experiment by Figdraw. P0 refers to infecting MDCK cells with A/WSN/1933 at a multiplicity of infection (MOI) of 0.01. P ( n +1) refers to the infection of cells with the supernatant collected from P n , followed by the addition of the compound at its half-maximal effective concentration (EC 50 ) of P n. The EC 50 values were tested by cell counting kit 8 assay. (B) Shows the resistance curves of A5 , oseltamivir, and baloxavir. Data are mean ± SD, n = 3. (C) Is an antiviral curve of A5 against oseltamivir-resistant WSN. (D) Is an antiviral curve of A5 against baloxavir-resistant WSN. The curves were created with nonlinear regression analysis. Data are mean ± SD, n = 3.
Article Snippet: Zanamivir, oseltamivir phosphate, favipiravir, and baloxavir were purchased from MedChemExpress (NJ, USA).
Techniques: In Vitro, Infection, Concentration Assay, Cell Counting