investigational  (MedChemExpress)

Journal: International Journal of Biological Sciences

Article Title: Glucocorticoids improve severe or critical COVID-19 by activating ACE2 and reducing IL-6 levels

doi: 10.7150/ijbs.47652

Figure Lengend Snippet: The top 20 candidate ACE2 agonists

Article Snippet: Kinetin riboside, cilomilast, rigosertib, PMA, and LPS were purchased from MCE Co. IFN-γ was from PeproTech (New Jersey, USA).

Techniques: DNA Synthesis

Journal: International Journal of Biological Sciences

Article Title: Glucocorticoids improve severe or critical COVID-19 by activating ACE2 and reducing IL-6 levels

doi: 10.7150/ijbs.47652

Figure Lengend Snippet: Activation of ACE2 expression and inhibition of IL-6 secretion in macrophages using glucocorticoids or other chemicals. A ) Several glucocorticoids and candidate agonists activated ACE2 expression in M0 macrophages, whereas only glucocorticoids imparted an activating effect on M1 macrophages. B) Low-dose hydrocortisone within the range of 10 to 100 nm is sufficient in activating ACE2 expression in macrophages. C) All glucocorticoids imparted a suppressive effect on IL-6 secretion. Dexamethasone revealed the strongest activating effect, followed by hydrocortisone, methylprednisolone, triamcinolone, and prednisolone. The chemical rigosertib showed a suppressive effect on IL-6 production to some extent. D) Low-dose hydrocortisone within the range of 50 to 100 nm is sufficient in suppressing IL-6 secretion in M1 macrophages. “*” represents a significant difference compared to the “control” or “0 nm” group. *P < 0.05, **P < 0.01, ***P < 0.001.

Article Snippet: Kinetin riboside, cilomilast, rigosertib, PMA, and LPS were purchased from MCE Co. IFN-γ was from PeproTech (New Jersey, USA).

Techniques: Activation Assay, Expressing, Inhibition, Control