lipofermata  (MedChemExpress)

Journal: The Journal of Clinical Investigation

Article Title: Fatty acid transport protein 2 inhibition enhances glucose tolerance through α cell–mediated GLP-1 secretion

doi: 10.1172/JCI192011

Figure Lengend Snippet: ( A and B ) FATP2 and loading control GAPDH mRNA expression was determined in human and mouse pancreatic tissue and α cell lines by RT-PCR (as described in Methods). Data are representative of 3 experiments per condition. ( C ) Mouse αTC1-6 cells were preincubated with lipofermata (1 hour, 37°C, 0–50 μM) in triplicate. BODIPY-labeled fatty acid uptake velocity was then determined, as described in Methods. Results show the mean ± SEM of 4 experiments.

Article Snippet: Wells were washed with serum-free, phenol-free media for 2 hours at 37° C. Lipofermata (5-bromo-5′-phenylspiro[3 H -1,3,4-thiadiazole-2,3′-indoline]-2-1) (MedChemExpress) was robotically incubated for the final hour.

Techniques: Control, Expressing, Reverse Transcription Polymerase Chain Reaction, Labeling

Journal: The Journal of Clinical Investigation

Article Title: Fatty acid transport protein 2 inhibition enhances glucose tolerance through α cell–mediated GLP-1 secretion

doi: 10.1172/JCI192011

Figure Lengend Snippet: ( A ) Pancreatic GLP-1 + α cell mass was determined as described in Methods in db/db and FATP2-KO db/db mice. * P < 0.01 compared with the db/db group by t test. ( B ) Human islets were preincubated with or without lipofermata (LF) and then tested for glucose-stimulated GLP-1 secretion as described in Methods. * P < 0.01 compared with all other groups by ANOVA. ( C ) αTC1-6 cells were preincubated with or without lipofermata or palmitate (Palm) as indicated. Glucose-stimulated GLP-1 secretion was then measured as described in Methods. Each symbol in the scatter bars in B and C represents 1 sample that was assayed in duplicate ( n = 3–6 samples per condition). * P < 0.05 compared lipofermata plus palmitate by ANOVA. ( D ) αTC1-6 cells coincubated in 5 mM or 25 mM glucose with or without 400 μM palmitate with or without 50 μM lipofermata for 16 hours were analyzed for Pcsk1 and Pcsk2 mRNA expression by qPCR. The results are expressed as the ratio relative to the 5 mM glucose-only condition. * P < 0.05 compared with other groups by ANOVA. ( E ) Glucose-stimulated insulin secretion was measured in human islets, which were preincubated with or without lipofermata and then exposed to exendin[9-39] (Ex) or palmitate, as described in Methods. Each symbol in the scatter bars represents 1 sample that was assayed in duplicate ( n = 3 samples per condition). * P < 0.01 compared with 16.8 mM glucose plus the lipofermata group by ANOVA. Data represent the mean ± SEM.

Article Snippet: Wells were washed with serum-free, phenol-free media for 2 hours at 37° C. Lipofermata (5-bromo-5′-phenylspiro[3 H -1,3,4-thiadiazole-2,3′-indoline]-2-1) (MedChemExpress) was robotically incubated for the final hour.

Techniques: Expressing