ccr1 antagonist j 113863  (MedChemExpress)

Journal: Frontiers in Molecular Biosciences

Article Title: Multitargeted biological actions of polydatin in preventing pseudogout acute attack

doi: 10.3389/fmolb.2025.1553912

Figure Lengend Snippet: Effect of IL-1β signal inhibition on PD treatment. Monocytes were pretreated with PD (100 µM) for 2 h and then stimulated for 24 h with CPP (0.025 mg/mL). Where indicated, anakinra (anak, 0.1 µg/mL) was added 30 min before the PD treatment. (A) IL-1β, (B) IL-18, (C) IL-6, (D) TNFα, (E) IL-8, (F) CCL-23 and (G) VEGF levels in supernatants were quantified by ELISA. Data are expressed as mean of four independent experiments ±SD. p calculated according to the One Way Anova, Bonferroni’s multiple comparisons test: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. p calculated according to t-test, PD + CPP vs anak + PD + CPP: °p < 0.05, °°p < 0.01. CPP, calcium pyrophosphate crystals; PD, polydatin; anak, anakinra.

Article Snippet: Where indicated, polydatin (100 µM) was added 2 h before the stimulation with crystals and anakinra (0.1 µg/mL), J113863 (Medchemexpress, Monmouth Junction, USA, 10 µM) and EX527 (Selleckchem, Cologne, 10 µM) were added 30 min before the use of polydatin.

Techniques: Inhibition, Enzyme-linked Immunosorbent Assay

Journal: Frontiers in Molecular Biosciences

Article Title: Multitargeted biological actions of polydatin in preventing pseudogout acute attack

doi: 10.3389/fmolb.2025.1553912

Figure Lengend Snippet: Effect of CCR1 inhibition on PD treatment. Monocytes were pretreated with PD (100 µM) for 2 h and then stimulated for 24 h with CPP (0.025 mg/mL). Where indicated, J113863 (10 µM) was added 30 min before the PD treatment. (A) IL-1β, (B) IL-18, (C) IL-6, (D) TNFα, (E) IL-8, (F) VEGF levels in supernatants were quantified by ELISA. Data are expressed as mean of four independent experiments ±SD. p calculated according to the One Way Anova, Bonferroni’s multiple comparisons test:*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. p calculated according to t-test, PD + CPP vs J + PD + CPP: °°p < 0.01. CPP, calcium pyrophosphate crystals; PD, polydatin.

Article Snippet: Where indicated, polydatin (100 µM) was added 2 h before the stimulation with crystals and anakinra (0.1 µg/mL), J113863 (Medchemexpress, Monmouth Junction, USA, 10 µM) and EX527 (Selleckchem, Cologne, 10 µM) were added 30 min before the use of polydatin.

Techniques: Inhibition, Enzyme-linked Immunosorbent Assay

Journal: Frontiers in Molecular Biosciences

Article Title: Multitargeted biological actions of polydatin in preventing pseudogout acute attack

doi: 10.3389/fmolb.2025.1553912

Figure Lengend Snippet: Migration of PBMCs exposed to SFs from patients with CPP induced arthritis in the presence or absence of PD (100–200 µM) or J113866 (10 µM) for 1.30 h. Medium RMPI supplemented with 5% plasma was used as a positive control (C+), while medium RPMI supplemented with 1% FBS was used as a negative control (C-). Left panel : Representative image of migrated cells on the bottom of a filter membrane of a modified 48-well Boyden chamber. Right panel : Effect of 100–200 μM PD on PBMCs migration induced by (A) 5% plasma (n = 3) (B) inflammatory SFs from CIA patients (n = 4). (C) Effect of 10 μM J113863 on PBMCs migration induced by inflammatory SFs from CIA patients (n = 4). Cell migration is shown as optical density values (O.D). Each sample was tested in sextuplicate. Data are expressed as mean of three to four independent experiments ±SD. p calculated according to the One Way Anova, Bonferroni’s multiple comparisons test: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. CPP, calcium pyrophosphate crystals; PD, polydatin; SF, synovial fluid; PBMCs, peripheral blood mononuclear cells.

Article Snippet: Where indicated, polydatin (100 µM) was added 2 h before the stimulation with crystals and anakinra (0.1 µg/mL), J113863 (Medchemexpress, Monmouth Junction, USA, 10 µM) and EX527 (Selleckchem, Cologne, 10 µM) were added 30 min before the use of polydatin.

Techniques: Migration, Positive Control, Negative Control, Membrane, Modification