sigma 2 receptor antagonist 1  (MedChemExpress)

Journal: Journal of Cellular and Molecular Medicine

Article Title: The Sigma‐2 Receptor/TMEM97 Agonist PB28 Suppresses Cell Proliferation and Invasion by Regulating the PI3K‐AKT‐mTOR Signalling Pathway in Renal Cancer

doi: 10.1111/jcmm.17047

Figure Lengend Snippet: Effects of PB28 or sigma‐2 receptor antagonist 1on the proliferation of renal cancer cells. Colony‐forming assay and RTCA assay were used to evaluate the proliferative ability of renal cancer cells. A The clonogenicity of 786‐O and ACHN cells showed a significant decrease when the cells cultured with PB28 (786‐O: p = 0.038, ACHN: p = 0.031). C The RTCA analysis also demonstrated an inhibitory effect of PB28 on renal cancer cells. B The colony‐forming abilities of 786‐O and ACHN cells increased significantly after sigma‐2 receptor inhibition for 8–10 days ( p = 0.0249 and p = 0.0153). D The RTCA analysis showed an facilitate effect of sigma‐2 receptor antagonist 1 on renal cancer cells. In the figure, PB represents PB28. YI represents sigma‐2 receptor antagonist 1. * represents p < 0.01

Article Snippet: PB28 (Santa Cruz Biotechnology, USA) is a highly selective agonist of sigma‐2, and sigma‐2 receptor antagonist 1 is a sigma‐2 receptor antagonist purchased from MedChemExpress (New Jersey, USA).

Techniques: Cell Culture, Inhibition

Journal: Journal of Cellular and Molecular Medicine

Article Title: The Sigma‐2 Receptor/TMEM97 Agonist PB28 Suppresses Cell Proliferation and Invasion by Regulating the PI3K‐AKT‐mTOR Signalling Pathway in Renal Cancer

doi: 10.1111/jcmm.17047

Figure Lengend Snippet: Effects of PB28 on the invasion or migration of renal cancer cells. Matrigel‐coated membranes were used to show the invasive ability of malignant renal cancer cells. A PB28 at 5 μM significantly reduced the invasion of both 786‐O and ACHN cells as revealed by the transwell assay (786‐O: p = 0.0356; ACHN: p = 0.024). B 786‐O and ACHN cells were seeded in 24‐well plates and scratched using a 1 ml pipette tip upon reaching confluence. Cell migration into the wound area was measured. Cells were treated with the sigma‐2 agonist PB28 and subjected to in vitro wound healing assays. Images were captured by an inverted light microscope. After comparing scratch widths to verify the migratory ability of cells treated with the compounds listed above at different concentrations, cells were counted after 24 h. PB28 obviously inhibited the migration of both 786‐O and ACHN cells at concentrations of 10 μM (P values were 786‐O: 0.0355, ACHN: 0.0477). In this figure, PB represents PB28

Article Snippet: PB28 (Santa Cruz Biotechnology, USA) is a highly selective agonist of sigma‐2, and sigma‐2 receptor antagonist 1 is a sigma‐2 receptor antagonist purchased from MedChemExpress (New Jersey, USA).

Techniques: Migration, Transwell Assay, Transferring, In Vitro, Light Microscopy

Journal: Journal of Cellular and Molecular Medicine

Article Title: The Sigma‐2 Receptor/TMEM97 Agonist PB28 Suppresses Cell Proliferation and Invasion by Regulating the PI3K‐AKT‐mTOR Signalling Pathway in Renal Cancer

doi: 10.1111/jcmm.17047

Figure Lengend Snippet: Effects of sigma‐2 receptor antagonist 1 on the invasion or migration of renal cancer cells. A Cells cultured with sigma‐2 receptor antagonist 1 (concentrations of 300 nM) greatly increased the invasive ability of 786‐O and ACHN cells, respectively. The P values were as follows: 786‐O, 0.0037 and ACHN, 0.0104. B Sigma‐2 receptor antagonist 1 exhibited a stimulative effect towards 786‐O and ACHN cells at concentrations of 300 nM. The P values were all significantly less than 0.05 (786‐O: 0.0322, ACHN: 0.0127). In the figure, YI represents sigma‐2 receptor antagonist 1

Article Snippet: PB28 (Santa Cruz Biotechnology, USA) is a highly selective agonist of sigma‐2, and sigma‐2 receptor antagonist 1 is a sigma‐2 receptor antagonist purchased from MedChemExpress (New Jersey, USA).

Techniques: Migration, Cell Culture