4 ipp  (MedChemExpress)

Journal: bioRxiv

Article Title: Single-cell profiling of synchronous multi-organ metastasis reveals a systemic CD74 + lipid-associated macrophage niche driving polymetastatic breast cancer

doi: 10.64898/2026.01.31.701004

Figure Lengend Snippet: a. Quantification of metastatic burden using ex vivo BLI of FVB mice injected VO-PyMT cells transduced with control vectors (shControl) and Mif-knockdown vectors (shMif 1; shMif 2). Data was acquired at day 12 post i.c. injections. shControl, n = 11; shMif (1), n = 11; shMif (2), n = 5. Data represents results from 2 independent experiments. In each experiment, raw photon flux values (p/s) from each organ were normalized to the average photon flux (p/s) values of the shControl group. Boxes depict 25th and 75th percentiles. Median is shown as horizontal lines. Whiskers depict data range. All data points are shown as dots. P values were determined using two-tailed Mann-Whitney tests by comparing shControl ( n = 11) versus the two shMif groups combined ( n = 16). Images show representative ex vivo BLI in brain, lung, liver or lower limb bones from each experimental group. b. Quantification of multi-organ metastatic burden ex vivo using BLI in albino C57/BL6 mice injected intracardially with Py8119 breast cancer cells transduced with control (shControl) and Mif-knockdown vector (shMif 2). Representative images are shown for each organ analyzed. Ex vivo metastatic burden was quantified 10 days after cancer cell injection. shControl, n = 14; shMif (2), n = 14. Data is representative of 3 independent experiments. Data in each organ was normalized to the average photon flux (p/s) of the shControl group in each experiment. Boxes boundaries define the interquartile ranges. Horizontal lines depict median values in each group. The whiskers show data range. The dots depict data points. P values were calculated by one-tailed Mann-Whitney t tests. c. Schematic showing pre-clinical model of MIF-CD74 targeting using 4-IPP, a small molecule inhibitor of MIF binding capacity. Multi-metastatic VO-PyMT cells were injected intracardially at day 0. Seeding and micrometastatic growth was allowed for 3 days, followed by i.p. administration of either a vehicle solution or 4-IPP. At day 12 post i.c. injection of VO-PyMT cells, metastatic colonization was analyzed ex vivo in brain, lung, liver and lower limb bones. d. Box plots showing quantification of metastatic burden of experiment as determined by ex vivo BLI in brain, lung, liver and bones of FVB mice injected intracardially with VO-PyMT cells and treated with the MIF inhibitor 4-IPP as described in (c). Vehicle group, n = 14; 4-IPP group, n = 13. For bone metastatic burden, right and left lower limb bones were analyzed separately. Data represents results from 2 independent experiments. In each experiment, photon flux (p/s) in each organ was normalized to the average photon flux (p/s) in the Vehicle control group. Boxes boundaries indicate 25 th and 75 th percentiles. Median is indicated by a horizontal line in boxes. Whiskers show data range. Data points are indicated as dots. P values were calculated using one-tailed Mann-Whitney t tests.

Article Snippet: For MIF-CD74 axis disruption in vivo , 4-IPP (MedChemExpress, cat no HY-110063) was first prepared in a vehicle solution consisting of 5% DMSO, 45% polyethylene glycol 300 (PEG300, MedChemExpress, cat no HY-Y0873), and 45% normal saline (0.9% NaCl).

Techniques: Ex Vivo, Injection, Transduction, Control, Knockdown, Two Tailed Test, MANN-WHITNEY, Plasmid Preparation, One-tailed Test, Binding Assay