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e2609  (MedChemExpress)


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    Structured Review

    MedChemExpress e2609
    (A) Cellular BACE1 levels were measured by immunoblot after treatment of HEK293-sw cells with 25 μg/ml cycloheximide together with either DMSO or 2.5 μM AZD3293 for 0.5, 1, 2, 4 and 6 h. α-Tubulin serves as loading control. (B-C) Cellular BACE1 levels were measured by ELISA after treatment of HEK293-sw cells with 25 μg/ml cycloheximide together with either DMSO or 0.1 μM L685,458 (negative control) or else 0.1 μM of six different BACE1 inhibitors (LY2811376, AZD3839, AZD3293, PF −06751979, Inhibitor IV, <t>E2609)</t> for 0.5, 1, 2, 4 and 6 h. (n=3; means +/− SD) (D) BACE1 ELISA of FPLC fractions from HEK293-sw cells treated with AZD3293 for 24 h or else AZD3293 for 24 h followed by another 24 h wash out with medium only (n=2; means +/− SD). (E) Cellular BACE1 level were measured by immunoblot after treatment of rat primary cortical neurons (DIV15) with 25 μg/ml cycloheximide plus DMSO or 2.5 μM AZD3293 for 0.5, 1, 2, 4 and 6 h. SNAP25 serves as loading control.
    E2609, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e2609/product/MedChemExpress
    Average 90 stars, based on 2 article reviews
    e2609 - by Bioz Stars, 2026-02
    90/100 stars

    Images

    1) Product Images from "Multiple BACE1 Inhibitors Abnormally Increase the BACE1 Protein in Neurons by Prolonging its Half-life"

    Article Title: Multiple BACE1 Inhibitors Abnormally Increase the BACE1 Protein in Neurons by Prolonging its Half-life

    Journal: Alzheimer's & dementia : the journal of the Alzheimer's Association

    doi: 10.1016/j.jalz.2019.06.3918

    (A) Cellular BACE1 levels were measured by immunoblot after treatment of HEK293-sw cells with 25 μg/ml cycloheximide together with either DMSO or 2.5 μM AZD3293 for 0.5, 1, 2, 4 and 6 h. α-Tubulin serves as loading control. (B-C) Cellular BACE1 levels were measured by ELISA after treatment of HEK293-sw cells with 25 μg/ml cycloheximide together with either DMSO or 0.1 μM L685,458 (negative control) or else 0.1 μM of six different BACE1 inhibitors (LY2811376, AZD3839, AZD3293, PF −06751979, Inhibitor IV, E2609) for 0.5, 1, 2, 4 and 6 h. (n=3; means +/− SD) (D) BACE1 ELISA of FPLC fractions from HEK293-sw cells treated with AZD3293 for 24 h or else AZD3293 for 24 h followed by another 24 h wash out with medium only (n=2; means +/− SD). (E) Cellular BACE1 level were measured by immunoblot after treatment of rat primary cortical neurons (DIV15) with 25 μg/ml cycloheximide plus DMSO or 2.5 μM AZD3293 for 0.5, 1, 2, 4 and 6 h. SNAP25 serves as loading control.
    Figure Legend Snippet: (A) Cellular BACE1 levels were measured by immunoblot after treatment of HEK293-sw cells with 25 μg/ml cycloheximide together with either DMSO or 2.5 μM AZD3293 for 0.5, 1, 2, 4 and 6 h. α-Tubulin serves as loading control. (B-C) Cellular BACE1 levels were measured by ELISA after treatment of HEK293-sw cells with 25 μg/ml cycloheximide together with either DMSO or 0.1 μM L685,458 (negative control) or else 0.1 μM of six different BACE1 inhibitors (LY2811376, AZD3839, AZD3293, PF −06751979, Inhibitor IV, E2609) for 0.5, 1, 2, 4 and 6 h. (n=3; means +/− SD) (D) BACE1 ELISA of FPLC fractions from HEK293-sw cells treated with AZD3293 for 24 h or else AZD3293 for 24 h followed by another 24 h wash out with medium only (n=2; means +/− SD). (E) Cellular BACE1 level were measured by immunoblot after treatment of rat primary cortical neurons (DIV15) with 25 μg/ml cycloheximide plus DMSO or 2.5 μM AZD3293 for 0.5, 1, 2, 4 and 6 h. SNAP25 serves as loading control.

    Techniques Used: Western Blot, Enzyme-linked Immunosorbent Assay, Negative Control



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    e2609  (MedChemExpress)
    90
    MedChemExpress e2609
    (A) Cellular BACE1 levels were measured by immunoblot after treatment of HEK293-sw cells with 25 μg/ml cycloheximide together with either DMSO or 2.5 μM AZD3293 for 0.5, 1, 2, 4 and 6 h. α-Tubulin serves as loading control. (B-C) Cellular BACE1 levels were measured by ELISA after treatment of HEK293-sw cells with 25 μg/ml cycloheximide together with either DMSO or 0.1 μM L685,458 (negative control) or else 0.1 μM of six different BACE1 inhibitors (LY2811376, AZD3839, AZD3293, PF −06751979, Inhibitor IV, <t>E2609)</t> for 0.5, 1, 2, 4 and 6 h. (n=3; means +/− SD) (D) BACE1 ELISA of FPLC fractions from HEK293-sw cells treated with AZD3293 for 24 h or else AZD3293 for 24 h followed by another 24 h wash out with medium only (n=2; means +/− SD). (E) Cellular BACE1 level were measured by immunoblot after treatment of rat primary cortical neurons (DIV15) with 25 μg/ml cycloheximide plus DMSO or 2.5 μM AZD3293 for 0.5, 1, 2, 4 and 6 h. SNAP25 serves as loading control.
    E2609, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e2609/product/MedChemExpress
    Average 90 stars, based on 1 article reviews
    e2609 - by Bioz Stars, 2026-02
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) Cellular BACE1 levels were measured by immunoblot after treatment of HEK293-sw cells with 25 μg/ml cycloheximide together with either DMSO or 2.5 μM AZD3293 for 0.5, 1, 2, 4 and 6 h. α-Tubulin serves as loading control. (B-C) Cellular BACE1 levels were measured by ELISA after treatment of HEK293-sw cells with 25 μg/ml cycloheximide together with either DMSO or 0.1 μM L685,458 (negative control) or else 0.1 μM of six different BACE1 inhibitors (LY2811376, AZD3839, AZD3293, PF −06751979, Inhibitor IV, E2609) for 0.5, 1, 2, 4 and 6 h. (n=3; means +/− SD) (D) BACE1 ELISA of FPLC fractions from HEK293-sw cells treated with AZD3293 for 24 h or else AZD3293 for 24 h followed by another 24 h wash out with medium only (n=2; means +/− SD). (E) Cellular BACE1 level were measured by immunoblot after treatment of rat primary cortical neurons (DIV15) with 25 μg/ml cycloheximide plus DMSO or 2.5 μM AZD3293 for 0.5, 1, 2, 4 and 6 h. SNAP25 serves as loading control.

    Journal: Alzheimer's & dementia : the journal of the Alzheimer's Association

    Article Title: Multiple BACE1 Inhibitors Abnormally Increase the BACE1 Protein in Neurons by Prolonging its Half-life

    doi: 10.1016/j.jalz.2019.06.3918

    Figure Lengend Snippet: (A) Cellular BACE1 levels were measured by immunoblot after treatment of HEK293-sw cells with 25 μg/ml cycloheximide together with either DMSO or 2.5 μM AZD3293 for 0.5, 1, 2, 4 and 6 h. α-Tubulin serves as loading control. (B-C) Cellular BACE1 levels were measured by ELISA after treatment of HEK293-sw cells with 25 μg/ml cycloheximide together with either DMSO or 0.1 μM L685,458 (negative control) or else 0.1 μM of six different BACE1 inhibitors (LY2811376, AZD3839, AZD3293, PF −06751979, Inhibitor IV, E2609) for 0.5, 1, 2, 4 and 6 h. (n=3; means +/− SD) (D) BACE1 ELISA of FPLC fractions from HEK293-sw cells treated with AZD3293 for 24 h or else AZD3293 for 24 h followed by another 24 h wash out with medium only (n=2; means +/− SD). (E) Cellular BACE1 level were measured by immunoblot after treatment of rat primary cortical neurons (DIV15) with 25 μg/ml cycloheximide plus DMSO or 2.5 μM AZD3293 for 0.5, 1, 2, 4 and 6 h. SNAP25 serves as loading control.

    Article Snippet: Reagents LY2886721, LY2811376, AZD3293, AZD3839, MK-8931, Pepstatin A, TAPI-1, Batimastat and DAPT were from Selleckchem; E2609 was from Sun-Shine Chemical; PF-06751979 was from MedChemExpree; β-secretase inhibitor IV was from Millipore; GI 254023X was from TOCRIS; Roburic acid was from Aobious; synthetic β-amyloid peptides are all from Anasepc; and recombinant human BACEl and BACE2 protein was from R&D system.

    Techniques: Western Blot, Enzyme-linked Immunosorbent Assay, Negative Control