colivelin tfa  (MedChemExpress)

Journal: Cellular and Molecular Immunology

Article Title: γδ + T-cell-derived IL-17A stimulates airway epithelial/stromal cells to secrete G-CSF, promoting lung-specific pathogenic Siglec-F + neutrophil development in PPE-induced emphysema

doi: 10.1038/s41423-025-01301-x

Figure Lengend Snippet: Signaling pathways downstream of the G-CSF receptor involved in the development of Siglec-F + neutrophils. The frequency of Siglec-F + neutrophils was assessed in BM-neutrophil cultures after 48 h of incubation with recombinant G-CSF in the presence of AG490 (JAK2 inhibitor) A , HJC0152 (STAT3 inhibitor) B , wortmannin (PI3K inhibitor) C , rapamycin (mTOR inhibitor) D , or adezmapimod (p38 inhibitor) E , n = 4 per group in each experiment. Unpaired two-tailed Student’s t test with Welch’s correction was used to measure significance. *** P < 0.001; ns not significant; error bars indicate the mean ± SD

Article Snippet: To block the differentiation of Siglec-F + neutrophils, the mice were i.t . administered 30 μg of AG490, 20 μg of HJC0152, 3 μg of rapamycin, or 37 μg of adzmapimod (MedChem Express) daily for 3 days, starting from the day after PPE instillation.

Techniques: Protein-Protein interactions, Incubation, Recombinant, Two Tailed Test

Journal: Cellular and Molecular Immunology

Article Title: γδ + T-cell-derived IL-17A stimulates airway epithelial/stromal cells to secrete G-CSF, promoting lung-specific pathogenic Siglec-F + neutrophil development in PPE-induced emphysema

doi: 10.1038/s41423-025-01301-x

Figure Lengend Snippet: Depletion of Siglec-F + neutrophils or inhibition of G-CSFR signaling alleviated emphysema symptoms. WT mice were treated i.t . with anti-Ly6G antibody A or anti-Siglec-F antibody B before instillation with PPE. Lung tissue histology after H&E staining and MLI assessment were conducted 4 days after PPE instillation. Scale bar = 200 μm ( n = 4 per group in each experiment). PPE-instilled mice were treated i.t . with AG490 (JAK2 inhibitor) C , HJC0152 (STAT3 inhibitor) D , rapamycin (mTOR inhibitor) E , or Adezmapimod (p38 inhibitor) F . Lung tissue histology after H&E staining and MLI assessment were conducted 4 days after PPE instillation. Scale bar = 200 μm ( n = 4 per group). Unpaired two-tailed Student’s t test with Welch’s correction was used to measure significance. * P < 0.05, ** P < 0.01, *** P < 0.001; the error bars indicate the means ± SDs

Article Snippet: To block the differentiation of Siglec-F + neutrophils, the mice were i.t . administered 30 μg of AG490, 20 μg of HJC0152, 3 μg of rapamycin, or 37 μg of adzmapimod (MedChem Express) daily for 3 days, starting from the day after PPE instillation.

Techniques: Inhibition, Staining, Two Tailed Test