Review



hif 1α inhibitor acriflavine  (MedChemExpress)


Bioz Verified Symbol MedChemExpress is a verified supplier
Bioz Manufacturer Symbol MedChemExpress manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 94
      Buy from Supplier

    Structured Review

    MedChemExpress hif 1α inhibitor acriflavine
    GPR43 deficiency promotes macrophage M1 polarization via the <t>HIF-1α–ENO1</t> axis. A Representative immunoblots of iNOS and ENO1 in WT and Gpr43 −/− BMDMs ± AP-III-a4 (ENO1 inhibitor, 20 µM) for 6 h. The protein levels are shown in Supplementary Fig. S6A. B , C Flow cytometry analysis of iNOS expression in M1-polarized WT and Gpr43 −/− BMDMs ± AP-III-a4 ( n = 3). Representative flow cytometry histogram ( B ) and the iNOS MFI quantification ( C ). D Lactate production in M1-polarized WT and Gpr43 −/− BMDMs ± AP-III-a4 ( n = 3). E Supernatant cytokines measured by ELISA in M1-polarized WT and Gpr43 −/− BMDMs ± AP-III-a4 ( n = 3). F Kaplan–Meier survival curves for WT and Gpr43 −/− mice after CLP ± AP-III-a4 (10 mg/kg) ( n = 10). G – I Lung histopathology and immunofluorescence after CLP in WT and Gpr43 −/− mice ± AP-III-a4 ( n = 6). Representative H&E and immunofluorescence images ( G , iNOS, green; F4/80, red; nuclei, DAPI, blue). Lung injury scores ( H ) and quantification of iNOS mean fluorescence intensity ( I ). J Representative immunoblots of HIF-1α, c-Myc, p-AKT and p-mTOR expression in M1-polarized WT and Gpr43 −/− BMDMs. The protein levels are shown in Supplementary Fig. S6B. K , L Flow cytometry analysis of iNOS expression in M1-polarized WT and Gpr43 −/− BMDMs ± the HIF-1α inhibitor acriflavine (5 μM) for 6 h ( n = 3). Representative histogram of iNOS expression ( K ) and quantification of the iNOS MFI ( L ). M Supernatant cytokines measured by ELISA in M1-polarized WT and Gpr43 −/− BMDMs ± acriflavine ( n = 3). Data are presented as means ± SD. Two-group comparisons used two-tailed unpaired Student’s t -test; multiple groups used one-way ANOVA with Tukey’s post hoc test; survival by log-rank test. Data in C – E and L – M were obtained from three independent experiments. Data in H , I are representative of three independent experiments. ns, not significant; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001
    Hif 1α Inhibitor Acriflavine, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hif 1α inhibitor acriflavine/product/MedChemExpress
    Average 94 stars, based on 10 article reviews
    hif 1α inhibitor acriflavine - by Bioz Stars, 2026-02
    94/100 stars

    Images

    1) Product Images from "GPR43 deficiency aggravates sepsis by promoting gut microbiota–dependent barrier disruption and HIF-1α–ENO1 axis–mediated M1 polarization of macrophages"

    Article Title: GPR43 deficiency aggravates sepsis by promoting gut microbiota–dependent barrier disruption and HIF-1α–ENO1 axis–mediated M1 polarization of macrophages

    Journal: Cellular & Molecular Biology Letters

    doi: 10.1186/s11658-025-00833-4

    GPR43 deficiency promotes macrophage M1 polarization via the HIF-1α–ENO1 axis. A Representative immunoblots of iNOS and ENO1 in WT and Gpr43 −/− BMDMs ± AP-III-a4 (ENO1 inhibitor, 20 µM) for 6 h. The protein levels are shown in Supplementary Fig. S6A. B , C Flow cytometry analysis of iNOS expression in M1-polarized WT and Gpr43 −/− BMDMs ± AP-III-a4 ( n = 3). Representative flow cytometry histogram ( B ) and the iNOS MFI quantification ( C ). D Lactate production in M1-polarized WT and Gpr43 −/− BMDMs ± AP-III-a4 ( n = 3). E Supernatant cytokines measured by ELISA in M1-polarized WT and Gpr43 −/− BMDMs ± AP-III-a4 ( n = 3). F Kaplan–Meier survival curves for WT and Gpr43 −/− mice after CLP ± AP-III-a4 (10 mg/kg) ( n = 10). G – I Lung histopathology and immunofluorescence after CLP in WT and Gpr43 −/− mice ± AP-III-a4 ( n = 6). Representative H&E and immunofluorescence images ( G , iNOS, green; F4/80, red; nuclei, DAPI, blue). Lung injury scores ( H ) and quantification of iNOS mean fluorescence intensity ( I ). J Representative immunoblots of HIF-1α, c-Myc, p-AKT and p-mTOR expression in M1-polarized WT and Gpr43 −/− BMDMs. The protein levels are shown in Supplementary Fig. S6B. K , L Flow cytometry analysis of iNOS expression in M1-polarized WT and Gpr43 −/− BMDMs ± the HIF-1α inhibitor acriflavine (5 μM) for 6 h ( n = 3). Representative histogram of iNOS expression ( K ) and quantification of the iNOS MFI ( L ). M Supernatant cytokines measured by ELISA in M1-polarized WT and Gpr43 −/− BMDMs ± acriflavine ( n = 3). Data are presented as means ± SD. Two-group comparisons used two-tailed unpaired Student’s t -test; multiple groups used one-way ANOVA with Tukey’s post hoc test; survival by log-rank test. Data in C – E and L – M were obtained from three independent experiments. Data in H , I are representative of three independent experiments. ns, not significant; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001
    Figure Legend Snippet: GPR43 deficiency promotes macrophage M1 polarization via the HIF-1α–ENO1 axis. A Representative immunoblots of iNOS and ENO1 in WT and Gpr43 −/− BMDMs ± AP-III-a4 (ENO1 inhibitor, 20 µM) for 6 h. The protein levels are shown in Supplementary Fig. S6A. B , C Flow cytometry analysis of iNOS expression in M1-polarized WT and Gpr43 −/− BMDMs ± AP-III-a4 ( n = 3). Representative flow cytometry histogram ( B ) and the iNOS MFI quantification ( C ). D Lactate production in M1-polarized WT and Gpr43 −/− BMDMs ± AP-III-a4 ( n = 3). E Supernatant cytokines measured by ELISA in M1-polarized WT and Gpr43 −/− BMDMs ± AP-III-a4 ( n = 3). F Kaplan–Meier survival curves for WT and Gpr43 −/− mice after CLP ± AP-III-a4 (10 mg/kg) ( n = 10). G – I Lung histopathology and immunofluorescence after CLP in WT and Gpr43 −/− mice ± AP-III-a4 ( n = 6). Representative H&E and immunofluorescence images ( G , iNOS, green; F4/80, red; nuclei, DAPI, blue). Lung injury scores ( H ) and quantification of iNOS mean fluorescence intensity ( I ). J Representative immunoblots of HIF-1α, c-Myc, p-AKT and p-mTOR expression in M1-polarized WT and Gpr43 −/− BMDMs. The protein levels are shown in Supplementary Fig. S6B. K , L Flow cytometry analysis of iNOS expression in M1-polarized WT and Gpr43 −/− BMDMs ± the HIF-1α inhibitor acriflavine (5 μM) for 6 h ( n = 3). Representative histogram of iNOS expression ( K ) and quantification of the iNOS MFI ( L ). M Supernatant cytokines measured by ELISA in M1-polarized WT and Gpr43 −/− BMDMs ± acriflavine ( n = 3). Data are presented as means ± SD. Two-group comparisons used two-tailed unpaired Student’s t -test; multiple groups used one-way ANOVA with Tukey’s post hoc test; survival by log-rank test. Data in C – E and L – M were obtained from three independent experiments. Data in H , I are representative of three independent experiments. ns, not significant; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

    Techniques Used: Western Blot, Flow Cytometry, Expressing, Enzyme-linked Immunosorbent Assay, Histopathology, Immunofluorescence, Fluorescence, Two Tailed Test



    Similar Products

    hif 1α inhibitor acriflavine  (MedChemExpress)
    94
    MedChemExpress hif 1α inhibitor acriflavine
    GPR43 deficiency promotes macrophage M1 polarization via the <t>HIF-1α–ENO1</t> axis. A Representative immunoblots of iNOS and ENO1 in WT and Gpr43 −/− BMDMs ± AP-III-a4 (ENO1 inhibitor, 20 µM) for 6 h. The protein levels are shown in Supplementary Fig. S6A. B , C Flow cytometry analysis of iNOS expression in M1-polarized WT and Gpr43 −/− BMDMs ± AP-III-a4 ( n = 3). Representative flow cytometry histogram ( B ) and the iNOS MFI quantification ( C ). D Lactate production in M1-polarized WT and Gpr43 −/− BMDMs ± AP-III-a4 ( n = 3). E Supernatant cytokines measured by ELISA in M1-polarized WT and Gpr43 −/− BMDMs ± AP-III-a4 ( n = 3). F Kaplan–Meier survival curves for WT and Gpr43 −/− mice after CLP ± AP-III-a4 (10 mg/kg) ( n = 10). G – I Lung histopathology and immunofluorescence after CLP in WT and Gpr43 −/− mice ± AP-III-a4 ( n = 6). Representative H&E and immunofluorescence images ( G , iNOS, green; F4/80, red; nuclei, DAPI, blue). Lung injury scores ( H ) and quantification of iNOS mean fluorescence intensity ( I ). J Representative immunoblots of HIF-1α, c-Myc, p-AKT and p-mTOR expression in M1-polarized WT and Gpr43 −/− BMDMs. The protein levels are shown in Supplementary Fig. S6B. K , L Flow cytometry analysis of iNOS expression in M1-polarized WT and Gpr43 −/− BMDMs ± the HIF-1α inhibitor acriflavine (5 μM) for 6 h ( n = 3). Representative histogram of iNOS expression ( K ) and quantification of the iNOS MFI ( L ). M Supernatant cytokines measured by ELISA in M1-polarized WT and Gpr43 −/− BMDMs ± acriflavine ( n = 3). Data are presented as means ± SD. Two-group comparisons used two-tailed unpaired Student’s t -test; multiple groups used one-way ANOVA with Tukey’s post hoc test; survival by log-rank test. Data in C – E and L – M were obtained from three independent experiments. Data in H , I are representative of three independent experiments. ns, not significant; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001
    Hif 1α Inhibitor Acriflavine, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hif 1α inhibitor acriflavine/product/MedChemExpress
    Average 94 stars, based on 1 article reviews
    hif 1α inhibitor acriflavine - by Bioz Stars, 2026-02
    94/100 stars
    		  Buy from Supplier
    acriflavine  (MedChemExpress)
    94
    MedChemExpress acriflavine
    GPR43 deficiency promotes macrophage M1 polarization via the <t>HIF-1α–ENO1</t> axis. A Representative immunoblots of iNOS and ENO1 in WT and Gpr43 −/− BMDMs ± AP-III-a4 (ENO1 inhibitor, 20 µM) for 6 h. The protein levels are shown in Supplementary Fig. S6A. B , C Flow cytometry analysis of iNOS expression in M1-polarized WT and Gpr43 −/− BMDMs ± AP-III-a4 ( n = 3). Representative flow cytometry histogram ( B ) and the iNOS MFI quantification ( C ). D Lactate production in M1-polarized WT and Gpr43 −/− BMDMs ± AP-III-a4 ( n = 3). E Supernatant cytokines measured by ELISA in M1-polarized WT and Gpr43 −/− BMDMs ± AP-III-a4 ( n = 3). F Kaplan–Meier survival curves for WT and Gpr43 −/− mice after CLP ± AP-III-a4 (10 mg/kg) ( n = 10). G – I Lung histopathology and immunofluorescence after CLP in WT and Gpr43 −/− mice ± AP-III-a4 ( n = 6). Representative H&E and immunofluorescence images ( G , iNOS, green; F4/80, red; nuclei, DAPI, blue). Lung injury scores ( H ) and quantification of iNOS mean fluorescence intensity ( I ). J Representative immunoblots of HIF-1α, c-Myc, p-AKT and p-mTOR expression in M1-polarized WT and Gpr43 −/− BMDMs. The protein levels are shown in Supplementary Fig. S6B. K , L Flow cytometry analysis of iNOS expression in M1-polarized WT and Gpr43 −/− BMDMs ± the HIF-1α inhibitor acriflavine (5 μM) for 6 h ( n = 3). Representative histogram of iNOS expression ( K ) and quantification of the iNOS MFI ( L ). M Supernatant cytokines measured by ELISA in M1-polarized WT and Gpr43 −/− BMDMs ± acriflavine ( n = 3). Data are presented as means ± SD. Two-group comparisons used two-tailed unpaired Student’s t -test; multiple groups used one-way ANOVA with Tukey’s post hoc test; survival by log-rank test. Data in C – E and L – M were obtained from three independent experiments. Data in H , I are representative of three independent experiments. ns, not significant; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001
    Acriflavine, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/acriflavine/product/MedChemExpress
    Average 94 stars, based on 1 article reviews
    acriflavine - by Bioz Stars, 2026-02
    94/100 stars
    		  Buy from Supplier
    hypoxia inducible factor 1α hif1α inhibitor acriflavine  (MedChemExpress)
    94
    MedChemExpress hypoxia inducible factor 1α hif1α inhibitor acriflavine
    GPR43 deficiency promotes macrophage M1 polarization via the <t>HIF-1α–ENO1</t> axis. A Representative immunoblots of iNOS and ENO1 in WT and Gpr43 −/− BMDMs ± AP-III-a4 (ENO1 inhibitor, 20 µM) for 6 h. The protein levels are shown in Supplementary Fig. S6A. B , C Flow cytometry analysis of iNOS expression in M1-polarized WT and Gpr43 −/− BMDMs ± AP-III-a4 ( n = 3). Representative flow cytometry histogram ( B ) and the iNOS MFI quantification ( C ). D Lactate production in M1-polarized WT and Gpr43 −/− BMDMs ± AP-III-a4 ( n = 3). E Supernatant cytokines measured by ELISA in M1-polarized WT and Gpr43 −/− BMDMs ± AP-III-a4 ( n = 3). F Kaplan–Meier survival curves for WT and Gpr43 −/− mice after CLP ± AP-III-a4 (10 mg/kg) ( n = 10). G – I Lung histopathology and immunofluorescence after CLP in WT and Gpr43 −/− mice ± AP-III-a4 ( n = 6). Representative H&E and immunofluorescence images ( G , iNOS, green; F4/80, red; nuclei, DAPI, blue). Lung injury scores ( H ) and quantification of iNOS mean fluorescence intensity ( I ). J Representative immunoblots of HIF-1α, c-Myc, p-AKT and p-mTOR expression in M1-polarized WT and Gpr43 −/− BMDMs. The protein levels are shown in Supplementary Fig. S6B. K , L Flow cytometry analysis of iNOS expression in M1-polarized WT and Gpr43 −/− BMDMs ± the HIF-1α inhibitor acriflavine (5 μM) for 6 h ( n = 3). Representative histogram of iNOS expression ( K ) and quantification of the iNOS MFI ( L ). M Supernatant cytokines measured by ELISA in M1-polarized WT and Gpr43 −/− BMDMs ± acriflavine ( n = 3). Data are presented as means ± SD. Two-group comparisons used two-tailed unpaired Student’s t -test; multiple groups used one-way ANOVA with Tukey’s post hoc test; survival by log-rank test. Data in C – E and L – M were obtained from three independent experiments. Data in H , I are representative of three independent experiments. ns, not significant; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001
    Hypoxia Inducible Factor 1α Hif1α Inhibitor Acriflavine, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hypoxia inducible factor 1α hif1α inhibitor acriflavine/product/MedChemExpress
    Average 94 stars, based on 1 article reviews
    hypoxia inducible factor 1α hif1α inhibitor acriflavine - by Bioz Stars, 2026-02
    94/100 stars
    		  Buy from Supplier
    acriflavine acf  (MedChemExpress)
    94
    MedChemExpress acriflavine acf
    GPR43 deficiency promotes macrophage M1 polarization via the <t>HIF-1α–ENO1</t> axis. A Representative immunoblots of iNOS and ENO1 in WT and Gpr43 −/− BMDMs ± AP-III-a4 (ENO1 inhibitor, 20 µM) for 6 h. The protein levels are shown in Supplementary Fig. S6A. B , C Flow cytometry analysis of iNOS expression in M1-polarized WT and Gpr43 −/− BMDMs ± AP-III-a4 ( n = 3). Representative flow cytometry histogram ( B ) and the iNOS MFI quantification ( C ). D Lactate production in M1-polarized WT and Gpr43 −/− BMDMs ± AP-III-a4 ( n = 3). E Supernatant cytokines measured by ELISA in M1-polarized WT and Gpr43 −/− BMDMs ± AP-III-a4 ( n = 3). F Kaplan–Meier survival curves for WT and Gpr43 −/− mice after CLP ± AP-III-a4 (10 mg/kg) ( n = 10). G – I Lung histopathology and immunofluorescence after CLP in WT and Gpr43 −/− mice ± AP-III-a4 ( n = 6). Representative H&E and immunofluorescence images ( G , iNOS, green; F4/80, red; nuclei, DAPI, blue). Lung injury scores ( H ) and quantification of iNOS mean fluorescence intensity ( I ). J Representative immunoblots of HIF-1α, c-Myc, p-AKT and p-mTOR expression in M1-polarized WT and Gpr43 −/− BMDMs. The protein levels are shown in Supplementary Fig. S6B. K , L Flow cytometry analysis of iNOS expression in M1-polarized WT and Gpr43 −/− BMDMs ± the HIF-1α inhibitor acriflavine (5 μM) for 6 h ( n = 3). Representative histogram of iNOS expression ( K ) and quantification of the iNOS MFI ( L ). M Supernatant cytokines measured by ELISA in M1-polarized WT and Gpr43 −/− BMDMs ± acriflavine ( n = 3). Data are presented as means ± SD. Two-group comparisons used two-tailed unpaired Student’s t -test; multiple groups used one-way ANOVA with Tukey’s post hoc test; survival by log-rank test. Data in C – E and L – M were obtained from three independent experiments. Data in H , I are representative of three independent experiments. ns, not significant; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001
    Acriflavine Acf, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/acriflavine acf/product/MedChemExpress
    Average 94 stars, based on 1 article reviews
    acriflavine acf - by Bioz Stars, 2026-02
    94/100 stars
    		  Buy from Supplier
    acf  (MedChemExpress)
    94
    MedChemExpress acf
    GPR43 deficiency promotes macrophage M1 polarization via the <t>HIF-1α–ENO1</t> axis. A Representative immunoblots of iNOS and ENO1 in WT and Gpr43 −/− BMDMs ± AP-III-a4 (ENO1 inhibitor, 20 µM) for 6 h. The protein levels are shown in Supplementary Fig. S6A. B , C Flow cytometry analysis of iNOS expression in M1-polarized WT and Gpr43 −/− BMDMs ± AP-III-a4 ( n = 3). Representative flow cytometry histogram ( B ) and the iNOS MFI quantification ( C ). D Lactate production in M1-polarized WT and Gpr43 −/− BMDMs ± AP-III-a4 ( n = 3). E Supernatant cytokines measured by ELISA in M1-polarized WT and Gpr43 −/− BMDMs ± AP-III-a4 ( n = 3). F Kaplan–Meier survival curves for WT and Gpr43 −/− mice after CLP ± AP-III-a4 (10 mg/kg) ( n = 10). G – I Lung histopathology and immunofluorescence after CLP in WT and Gpr43 −/− mice ± AP-III-a4 ( n = 6). Representative H&E and immunofluorescence images ( G , iNOS, green; F4/80, red; nuclei, DAPI, blue). Lung injury scores ( H ) and quantification of iNOS mean fluorescence intensity ( I ). J Representative immunoblots of HIF-1α, c-Myc, p-AKT and p-mTOR expression in M1-polarized WT and Gpr43 −/− BMDMs. The protein levels are shown in Supplementary Fig. S6B. K , L Flow cytometry analysis of iNOS expression in M1-polarized WT and Gpr43 −/− BMDMs ± the HIF-1α inhibitor acriflavine (5 μM) for 6 h ( n = 3). Representative histogram of iNOS expression ( K ) and quantification of the iNOS MFI ( L ). M Supernatant cytokines measured by ELISA in M1-polarized WT and Gpr43 −/− BMDMs ± acriflavine ( n = 3). Data are presented as means ± SD. Two-group comparisons used two-tailed unpaired Student’s t -test; multiple groups used one-way ANOVA with Tukey’s post hoc test; survival by log-rank test. Data in C – E and L – M were obtained from three independent experiments. Data in H , I are representative of three independent experiments. ns, not significant; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001
    Acf, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/acf/product/MedChemExpress
    Average 94 stars, based on 1 article reviews
    acf - by Bioz Stars, 2026-02
    94/100 stars
    		  Buy from Supplier
    hypoxia inducible factor inhibitor acriflavine  (MedChemExpress)
    94
    MedChemExpress hypoxia inducible factor inhibitor acriflavine
    GPR43 deficiency promotes macrophage M1 polarization via the <t>HIF-1α–ENO1</t> axis. A Representative immunoblots of iNOS and ENO1 in WT and Gpr43 −/− BMDMs ± AP-III-a4 (ENO1 inhibitor, 20 µM) for 6 h. The protein levels are shown in Supplementary Fig. S6A. B , C Flow cytometry analysis of iNOS expression in M1-polarized WT and Gpr43 −/− BMDMs ± AP-III-a4 ( n = 3). Representative flow cytometry histogram ( B ) and the iNOS MFI quantification ( C ). D Lactate production in M1-polarized WT and Gpr43 −/− BMDMs ± AP-III-a4 ( n = 3). E Supernatant cytokines measured by ELISA in M1-polarized WT and Gpr43 −/− BMDMs ± AP-III-a4 ( n = 3). F Kaplan–Meier survival curves for WT and Gpr43 −/− mice after CLP ± AP-III-a4 (10 mg/kg) ( n = 10). G – I Lung histopathology and immunofluorescence after CLP in WT and Gpr43 −/− mice ± AP-III-a4 ( n = 6). Representative H&E and immunofluorescence images ( G , iNOS, green; F4/80, red; nuclei, DAPI, blue). Lung injury scores ( H ) and quantification of iNOS mean fluorescence intensity ( I ). J Representative immunoblots of HIF-1α, c-Myc, p-AKT and p-mTOR expression in M1-polarized WT and Gpr43 −/− BMDMs. The protein levels are shown in Supplementary Fig. S6B. K , L Flow cytometry analysis of iNOS expression in M1-polarized WT and Gpr43 −/− BMDMs ± the HIF-1α inhibitor acriflavine (5 μM) for 6 h ( n = 3). Representative histogram of iNOS expression ( K ) and quantification of the iNOS MFI ( L ). M Supernatant cytokines measured by ELISA in M1-polarized WT and Gpr43 −/− BMDMs ± acriflavine ( n = 3). Data are presented as means ± SD. Two-group comparisons used two-tailed unpaired Student’s t -test; multiple groups used one-way ANOVA with Tukey’s post hoc test; survival by log-rank test. Data in C – E and L – M were obtained from three independent experiments. Data in H , I are representative of three independent experiments. ns, not significant; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001
    Hypoxia Inducible Factor Inhibitor Acriflavine, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hypoxia inducible factor inhibitor acriflavine/product/MedChemExpress
    Average 94 stars, based on 1 article reviews
    hypoxia inducible factor inhibitor acriflavine - by Bioz Stars, 2026-02
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    GPR43 deficiency promotes macrophage M1 polarization via the HIF-1α–ENO1 axis. A Representative immunoblots of iNOS and ENO1 in WT and Gpr43 −/− BMDMs ± AP-III-a4 (ENO1 inhibitor, 20 µM) for 6 h. The protein levels are shown in Supplementary Fig. S6A. B , C Flow cytometry analysis of iNOS expression in M1-polarized WT and Gpr43 −/− BMDMs ± AP-III-a4 ( n = 3). Representative flow cytometry histogram ( B ) and the iNOS MFI quantification ( C ). D Lactate production in M1-polarized WT and Gpr43 −/− BMDMs ± AP-III-a4 ( n = 3). E Supernatant cytokines measured by ELISA in M1-polarized WT and Gpr43 −/− BMDMs ± AP-III-a4 ( n = 3). F Kaplan–Meier survival curves for WT and Gpr43 −/− mice after CLP ± AP-III-a4 (10 mg/kg) ( n = 10). G – I Lung histopathology and immunofluorescence after CLP in WT and Gpr43 −/− mice ± AP-III-a4 ( n = 6). Representative H&E and immunofluorescence images ( G , iNOS, green; F4/80, red; nuclei, DAPI, blue). Lung injury scores ( H ) and quantification of iNOS mean fluorescence intensity ( I ). J Representative immunoblots of HIF-1α, c-Myc, p-AKT and p-mTOR expression in M1-polarized WT and Gpr43 −/− BMDMs. The protein levels are shown in Supplementary Fig. S6B. K , L Flow cytometry analysis of iNOS expression in M1-polarized WT and Gpr43 −/− BMDMs ± the HIF-1α inhibitor acriflavine (5 μM) for 6 h ( n = 3). Representative histogram of iNOS expression ( K ) and quantification of the iNOS MFI ( L ). M Supernatant cytokines measured by ELISA in M1-polarized WT and Gpr43 −/− BMDMs ± acriflavine ( n = 3). Data are presented as means ± SD. Two-group comparisons used two-tailed unpaired Student’s t -test; multiple groups used one-way ANOVA with Tukey’s post hoc test; survival by log-rank test. Data in C – E and L – M were obtained from three independent experiments. Data in H , I are representative of three independent experiments. ns, not significant; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

    Journal: Cellular & Molecular Biology Letters

    Article Title: GPR43 deficiency aggravates sepsis by promoting gut microbiota–dependent barrier disruption and HIF-1α–ENO1 axis–mediated M1 polarization of macrophages

    doi: 10.1186/s11658-025-00833-4

    Figure Lengend Snippet: GPR43 deficiency promotes macrophage M1 polarization via the HIF-1α–ENO1 axis. A Representative immunoblots of iNOS and ENO1 in WT and Gpr43 −/− BMDMs ± AP-III-a4 (ENO1 inhibitor, 20 µM) for 6 h. The protein levels are shown in Supplementary Fig. S6A. B , C Flow cytometry analysis of iNOS expression in M1-polarized WT and Gpr43 −/− BMDMs ± AP-III-a4 ( n = 3). Representative flow cytometry histogram ( B ) and the iNOS MFI quantification ( C ). D Lactate production in M1-polarized WT and Gpr43 −/− BMDMs ± AP-III-a4 ( n = 3). E Supernatant cytokines measured by ELISA in M1-polarized WT and Gpr43 −/− BMDMs ± AP-III-a4 ( n = 3). F Kaplan–Meier survival curves for WT and Gpr43 −/− mice after CLP ± AP-III-a4 (10 mg/kg) ( n = 10). G – I Lung histopathology and immunofluorescence after CLP in WT and Gpr43 −/− mice ± AP-III-a4 ( n = 6). Representative H&E and immunofluorescence images ( G , iNOS, green; F4/80, red; nuclei, DAPI, blue). Lung injury scores ( H ) and quantification of iNOS mean fluorescence intensity ( I ). J Representative immunoblots of HIF-1α, c-Myc, p-AKT and p-mTOR expression in M1-polarized WT and Gpr43 −/− BMDMs. The protein levels are shown in Supplementary Fig. S6B. K , L Flow cytometry analysis of iNOS expression in M1-polarized WT and Gpr43 −/− BMDMs ± the HIF-1α inhibitor acriflavine (5 μM) for 6 h ( n = 3). Representative histogram of iNOS expression ( K ) and quantification of the iNOS MFI ( L ). M Supernatant cytokines measured by ELISA in M1-polarized WT and Gpr43 −/− BMDMs ± acriflavine ( n = 3). Data are presented as means ± SD. Two-group comparisons used two-tailed unpaired Student’s t -test; multiple groups used one-way ANOVA with Tukey’s post hoc test; survival by log-rank test. Data in C – E and L – M were obtained from three independent experiments. Data in H , I are representative of three independent experiments. ns, not significant; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

    Article Snippet: For the inhibitor or agonist treatments, BMDMs were pretreated with glycolysis inhibitor 2-deoxyglucose (2-DG) (2 mM, 6 h, MCE, cat. no. HY-13966), ENO1 inhibitor AP-III-a4 (20 μM, 6 h, MCE, cat. no. HY-15858), HIF-1α inhibitor acriflavine (5 μM, 6 h, MCE, cat. no. HY-100575), or the GPR43 agonist TUG-1375 (20 μM, 6 h, MCE, cat. no. HY-112813) before M1 polarization.

    Techniques: Western Blot, Flow Cytometry, Expressing, Enzyme-linked Immunosorbent Assay, Histopathology, Immunofluorescence, Fluorescence, Two Tailed Test