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rsv 9320 strain  (ATCC)


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    ATCC rsv 9320 strain
    Rsv 9320 Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/9320s/Human+respiratory+syncytial+virus/pm41936817-68-1-3
    Average 95 stars, based on 39 article reviews
    rsv 9320 strain - by Bioz Stars, 2026-10
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    rsv 9320 strain  (ATCC)
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    Description of the evolution experiment workflow and model plasmid properties. (a) The novel kanamycin resistance allele ( nptII ) was introduced into a homoplasmic population of plasmid hosts where it integrates into a single plasmid copy in a subset of cells. The donor population was evolved by daily serial transfers; agitation prevents conjugation within the donor culture (Table S5). Cell type frequencies are quantified by ddPCR. Donors are mated daily with a plasmid-free recipient population on solid agar. Recipient genotypes are determined by differential plating. This design allows separate observation of conjugation and segregational drift. Plasmids copy number is supplied in the table below as the mean ±95% CI. The presence of partitioning system (Par) and toxin-antitoxin system (TA) is indicated. (b) Model plasmid stability in the Acinetobacter baylyi <t>BD413</t> host over 7 days (transfers). Error bars correspond to standard deviation. (c) Relative fitness w of homoplasmic ancestral hosts compared with plasmid-free hosts (with the following sample sizes: n pNCL =8; n pCLR =6; n pCHR =8) (left), homoplasmic novel allele host compared to plasmid-free cells (n pNCL =6; n pCLR =5; n pCHR =7) (middle), and homoplasmic ancestral allele hosts compared with homoplasmic novel allele hosts (n pNCL =7; n pCLR =5; n pCHR =8) (right). Error bars correspond to 95% CI; time series data of the measurements, linear regression analysis and relative fitness values are supplied in Fig. S1. d) Allele frequency as quantified by the established ddPCR protocol was validated by plating. Error bars correspond to 95% CI.
    Model Strain Acinetobacter Baylyi Bd413, supplied by DSMZ, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rsv strain 9320  (ATCC)
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    Description of the evolution experiment workflow and model plasmid properties. (a) The novel kanamycin resistance allele ( nptII ) was introduced into a homoplasmic population of plasmid hosts where it integrates into a single plasmid copy in a subset of cells. The donor population was evolved by daily serial transfers; agitation prevents conjugation within the donor culture (Table S5). Cell type frequencies are quantified by ddPCR. Donors are mated daily with a plasmid-free recipient population on solid agar. Recipient genotypes are determined by differential plating. This design allows separate observation of conjugation and segregational drift. Plasmids copy number is supplied in the table below as the mean ±95% CI. The presence of partitioning system (Par) and toxin-antitoxin system (TA) is indicated. (b) Model plasmid stability in the Acinetobacter baylyi <t>BD413</t> host over 7 days (transfers). Error bars correspond to standard deviation. (c) Relative fitness w of homoplasmic ancestral hosts compared with plasmid-free hosts (with the following sample sizes: n pNCL =8; n pCLR =6; n pCHR =8) (left), homoplasmic novel allele host compared to plasmid-free cells (n pNCL =6; n pCLR =5; n pCHR =7) (middle), and homoplasmic ancestral allele hosts compared with homoplasmic novel allele hosts (n pNCL =7; n pCLR =5; n pCHR =8) (right). Error bars correspond to 95% CI; time series data of the measurements, linear regression analysis and relative fitness values are supplied in Fig. S1. d) Allele frequency as quantified by the established ddPCR protocol was validated by plating. Error bars correspond to 95% CI.
    Rsv Strain 9320, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1x b 9320 atcc vr  (ATCC)
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    ATCC 1x b 9320 atcc vr
    Description of the evolution experiment workflow and model plasmid properties. (a) The novel kanamycin resistance allele ( nptII ) was introduced into a homoplasmic population of plasmid hosts where it integrates into a single plasmid copy in a subset of cells. The donor population was evolved by daily serial transfers; agitation prevents conjugation within the donor culture (Table S5). Cell type frequencies are quantified by ddPCR. Donors are mated daily with a plasmid-free recipient population on solid agar. Recipient genotypes are determined by differential plating. This design allows separate observation of conjugation and segregational drift. Plasmids copy number is supplied in the table below as the mean ±95% CI. The presence of partitioning system (Par) and toxin-antitoxin system (TA) is indicated. (b) Model plasmid stability in the Acinetobacter baylyi <t>BD413</t> host over 7 days (transfers). Error bars correspond to standard deviation. (c) Relative fitness w of homoplasmic ancestral hosts compared with plasmid-free hosts (with the following sample sizes: n pNCL =8; n pCLR =6; n pCHR =8) (left), homoplasmic novel allele host compared to plasmid-free cells (n pNCL =6; n pCLR =5; n pCHR =7) (middle), and homoplasmic ancestral allele hosts compared with homoplasmic novel allele hosts (n pNCL =7; n pCLR =5; n pCHR =8) (right). Error bars correspond to 95% CI; time series data of the measurements, linear regression analysis and relative fitness values are supplied in Fig. S1. d) Allele frequency as quantified by the established ddPCR protocol was validated by plating. Error bars correspond to 95% CI.
    1x B 9320 Atcc Vr, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    strain 9320  (ATCC)
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    Description of the evolution experiment workflow and model plasmid properties. (a) The novel kanamycin resistance allele ( nptII ) was introduced into a homoplasmic population of plasmid hosts where it integrates into a single plasmid copy in a subset of cells. The donor population was evolved by daily serial transfers; agitation prevents conjugation within the donor culture (Table S5). Cell type frequencies are quantified by ddPCR. Donors are mated daily with a plasmid-free recipient population on solid agar. Recipient genotypes are determined by differential plating. This design allows separate observation of conjugation and segregational drift. Plasmids copy number is supplied in the table below as the mean ±95% CI. The presence of partitioning system (Par) and toxin-antitoxin system (TA) is indicated. (b) Model plasmid stability in the Acinetobacter baylyi <t>BD413</t> host over 7 days (transfers). Error bars correspond to standard deviation. (c) Relative fitness w of homoplasmic ancestral hosts compared with plasmid-free hosts (with the following sample sizes: n pNCL =8; n pCLR =6; n pCHR =8) (left), homoplasmic novel allele host compared to plasmid-free cells (n pNCL =6; n pCLR =5; n pCHR =7) (middle), and homoplasmic ancestral allele hosts compared with homoplasmic novel allele hosts (n pNCL =7; n pCLR =5; n pCHR =8) (right). Error bars correspond to 95% CI; time series data of the measurements, linear regression analysis and relative fitness values are supplied in Fig. S1. d) Allele frequency as quantified by the established ddPCR protocol was validated by plating. Error bars correspond to 95% CI.
    Strain 9320, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    acinetobacter sp  (DSMZ)
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    DSMZ acinetobacter sp
    Description of the evolution experiment workflow and model plasmid properties. (a) The novel kanamycin resistance allele ( nptII ) was introduced into a homoplasmic population of plasmid hosts where it integrates into a single plasmid copy in a subset of cells. The donor population was evolved by daily serial transfers; agitation prevents conjugation within the donor culture (Table S5). Cell type frequencies are quantified by ddPCR. Donors are mated daily with a plasmid-free recipient population on solid agar. Recipient genotypes are determined by differential plating. This design allows separate observation of conjugation and segregational drift. Plasmids copy number is supplied in the table below as the mean ±95% CI. The presence of partitioning system (Par) and toxin-antitoxin system (TA) is indicated. (b) Model plasmid stability in the Acinetobacter baylyi <t>BD413</t> host over 7 days (transfers). Error bars correspond to standard deviation. (c) Relative fitness w of homoplasmic ancestral hosts compared with plasmid-free hosts (with the following sample sizes: n pNCL =8; n pCLR =6; n pCHR =8) (left), homoplasmic novel allele host compared to plasmid-free cells (n pNCL =6; n pCLR =5; n pCHR =7) (middle), and homoplasmic ancestral allele hosts compared with homoplasmic novel allele hosts (n pNCL =7; n pCLR =5; n pCHR =8) (right). Error bars correspond to 95% CI; time series data of the measurements, linear regression analysis and relative fitness values are supplied in Fig. S1. d) Allele frequency as quantified by the established ddPCR protocol was validated by plating. Error bars correspond to 95% CI.
    Acinetobacter Sp, supplied by DSMZ, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/9320s/Acinetobacter+sp/us12305179-292-67-77
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    Description of the evolution experiment workflow and model plasmid properties. (a) The novel kanamycin resistance allele ( nptII ) was introduced into a homoplasmic population of plasmid hosts where it integrates into a single plasmid copy in a subset of cells. The donor population was evolved by daily serial transfers; agitation prevents conjugation within the donor culture (Table S5). Cell type frequencies are quantified by ddPCR. Donors are mated daily with a plasmid-free recipient population on solid agar. Recipient genotypes are determined by differential plating. This design allows separate observation of conjugation and segregational drift. Plasmids copy number is supplied in the table below as the mean ±95% CI. The presence of partitioning system (Par) and toxin-antitoxin system (TA) is indicated. (b) Model plasmid stability in the Acinetobacter baylyi BD413 host over 7 days (transfers). Error bars correspond to standard deviation. (c) Relative fitness w of homoplasmic ancestral hosts compared with plasmid-free hosts (with the following sample sizes: n pNCL =8; n pCLR =6; n pCHR =8) (left), homoplasmic novel allele host compared to plasmid-free cells (n pNCL =6; n pCLR =5; n pCHR =7) (middle), and homoplasmic ancestral allele hosts compared with homoplasmic novel allele hosts (n pNCL =7; n pCLR =5; n pCHR =8) (right). Error bars correspond to 95% CI; time series data of the measurements, linear regression analysis and relative fitness values are supplied in Fig. S1. d) Allele frequency as quantified by the established ddPCR protocol was validated by plating. Error bars correspond to 95% CI.

    Journal: bioRxiv

    Article Title: Horizontal transfer promotes allele segregation in multicopy plasmids

    doi: 10.64898/2026.03.13.711586

    Figure Lengend Snippet: Description of the evolution experiment workflow and model plasmid properties. (a) The novel kanamycin resistance allele ( nptII ) was introduced into a homoplasmic population of plasmid hosts where it integrates into a single plasmid copy in a subset of cells. The donor population was evolved by daily serial transfers; agitation prevents conjugation within the donor culture (Table S5). Cell type frequencies are quantified by ddPCR. Donors are mated daily with a plasmid-free recipient population on solid agar. Recipient genotypes are determined by differential plating. This design allows separate observation of conjugation and segregational drift. Plasmids copy number is supplied in the table below as the mean ±95% CI. The presence of partitioning system (Par) and toxin-antitoxin system (TA) is indicated. (b) Model plasmid stability in the Acinetobacter baylyi BD413 host over 7 days (transfers). Error bars correspond to standard deviation. (c) Relative fitness w of homoplasmic ancestral hosts compared with plasmid-free hosts (with the following sample sizes: n pNCL =8; n pCLR =6; n pCHR =8) (left), homoplasmic novel allele host compared to plasmid-free cells (n pNCL =6; n pCLR =5; n pCHR =7) (middle), and homoplasmic ancestral allele hosts compared with homoplasmic novel allele hosts (n pNCL =7; n pCLR =5; n pCHR =8) (right). Error bars correspond to 95% CI; time series data of the measurements, linear regression analysis and relative fitness values are supplied in Fig. S1. d) Allele frequency as quantified by the established ddPCR protocol was validated by plating. Error bars correspond to 95% CI.

    Article Snippet: All experiments were performed using the model strain Acinetobacter baylyi BD413 and BD4 (DSM 588 and DSM 586; German Collection of Microorganisms and Cell Cultures, DSMZ).

    Techniques: Plasmid Preparation, Conjugation Assay, Standard Deviation