human tnbc cells 4175tr  (IDEXX)

Journal: Nature Communications

Article Title: DNA exonuclease Trex1 regulates radiotherapy-induced tumour immunogenicity

doi: 10.1038/ncomms15618

Figure Lengend Snippet: ( a , b ) Trex1 expression and cytoplasmic dsDNA accumulations in MDA-MB-231 ( a ) and 4175TR ( b ) breast carcinoma cells 24 hrs after in vitro treatment. Duplicate; * P <0.05; ** P <0.005; *** P <0.0005: t -test; n =3. All data are mean±s.e.m. ( c ) Trex1 , Ifnb1 and Mx1 expression measured by qRT-PCR in a patient-derived tumour xenograft (PDTX) 24 h after mice treatment with tumour-directed radiation. Duplicate; * P <0.05; ** P <0.005; *** P <0.0005: t -test; n =4. All data are mean±s.e.m.

Article Snippet: MDA-MB-231 and 4175TR human triple negative breast cancer cells were purchased from ATCC and obtained from J. Massague, respectively.

Techniques: Expressing, In Vitro, Quantitative RT-PCR, Derivative Assay

Journal: BMC Cancer

Article Title: Shortwave infrared emitting multicolored nanoprobes for biomarker-specific cancer imaging in vivo

doi: 10.1186/s12885-020-07604-8

Figure Lengend Snippet: Summary of targeted biomarkers and their relative expression levels in breast (MCF7, 4175TR) and ovarian (SKOV3) cancer cell lines. Also listed are the corresponding targeting ligands and nanoparticle compositions

Article Snippet: 1 × 10 4175TR cells were inoculated into the left dorsal flank and 5 × 10 6 MCF7 cells were inoculated in the right dorsal flank of 3–4-week-old female athymic nude mice (Taconic Biosciences, Hudson, NY) supplemented with 17β-estradiol pellets releasing estradiol at a rate of 16.66 μg/day.

Techniques: Expressing, Biomarker Discovery

Journal: BMC Cancer

Article Title: Shortwave infrared emitting multicolored nanoprobes for biomarker-specific cancer imaging in vivo

doi: 10.1186/s12885-020-07604-8

Figure Lengend Snippet: Targeting Validation of functionalized multi-color nanoprobes in vitro. Western blots indicate the relative expression levels of three different cancer biomarkers: a The 4175TR cell line shows increased CAV1 receptor expression relative to the MCF7 cell line. b MCF7 cells show higher CXCR4 expression than 4175TR cells. c SKOV3 ovarian cancer cell line used in this study were determined to be FR positive by immunoblotting. The western blots in a , b an c panels have been cropped to highlight the protein of interest in each panel. Panel a , b and c show western blots of caveolin-1, CXCR4 and folate receptor- alpha respectively. The full-length western blots are presented in Supplementary Fig. . d Flow cytometry shows a statistically significant increase in uptake of CAV1-targeted nanoprobes (Rh-fHoANCs) compared to untargeted nanoprobes (Rh-HoANCs) in CAV1-expressing 4175TR cells. No significant increase was observed in uptake of CAV1-targeted versus untargeted nanoprobes in CAV1-negative MCF7 cells. e CXCR4-targeted nanoprobes (FITC-fErANCs) showed significantly increased uptake in CXCR4-expressing MCF7 cells compared to untargeted nanoprobes (FITC-ErANCs). No significant increase was observed in uptake of CXCR4-targeted versus untargeted nanoprobes in CXCR4-negative 4175TR cells. f A statistically significant increase in uptake of FRα-targeted nanoprobes (647-fTmANCs) compared to untargeted nanoprobes (647-TmANCs) was observed in FRα-expressing SKOV3 cells. For panel d , e and f student t-test was used to determine statistical significance between two groups

Article Snippet: 1 × 10 4175TR cells were inoculated into the left dorsal flank and 5 × 10 6 MCF7 cells were inoculated in the right dorsal flank of 3–4-week-old female athymic nude mice (Taconic Biosciences, Hudson, NY) supplemented with 17β-estradiol pellets releasing estradiol at a rate of 16.66 μg/day.

Techniques: Biomarker Discovery, In Vitro, Western Blot, Expressing, Flow Cytometry

Journal: BMC Cancer

Article Title: Shortwave infrared emitting multicolored nanoprobes for biomarker-specific cancer imaging in vivo

doi: 10.1186/s12885-020-07604-8

Figure Lengend Snippet: Multi-Color Nanoprobe Targeting Validation in vivo. a Mice bearing subcutaneous 4175TR tumors received CAV1 targeted (Rh-fHoANC) and untargeted (Rh-HoANC) holmium-doped nanoprobes. Representative images of b Rh-HoANC and c Rh-fHoANC accumulation at 6-weeks post-inoculation. d SWIR signal intensities show brighter emissions from animals injected with targeted ( n = 7) compared to untargeted ( n = 5) nanoprobes. e Mice bearing subcutaneous MCF7 tumors received CXCR4 targeted (FITC-fErANC) and untargeted (FITC-ErANC) erbium-doped nanoprobes. Representative images of f FITC-ErANC and g FITC-fErANC accumulation. h SWIR signal intensities show brighter emissions from animals injected with targeted ( n = 8) compared to untargeted ( n = 5) nanoprobes. i Mice bearing subcutaneous SKOV3 tumors received folate receptor targeted (647-fTmANC) and untargeted (647-TmANC) thulium-doped nanoprobes. Representative images of j 647-TmANC and (k) 647-fTmANC accumulation. l SWIR signal intensities show brighter emissions from animals injected with targeted ( n = 10) compared to untargeted ( n = 6) nanoprobes. Bar graphs in panels d , h , and l represent the mean ± s.e.m. for each group. * p < 0.07, determined by a two-tailed Mann Whitney U-test; ** p < 0.05 determined by Welch’s two-tailed t-test. Panels b , c , f , g , j , and k each present two representative animals from each experimental group

Article Snippet: 1 × 10 4175TR cells were inoculated into the left dorsal flank and 5 × 10 6 MCF7 cells were inoculated in the right dorsal flank of 3–4-week-old female athymic nude mice (Taconic Biosciences, Hudson, NY) supplemented with 17β-estradiol pellets releasing estradiol at a rate of 16.66 μg/day.

Techniques: Biomarker Discovery, In Vivo, Injection, Two Tailed Test, MANN-WHITNEY

Journal: BMC Cancer

Article Title: Shortwave infrared emitting multicolored nanoprobes for biomarker-specific cancer imaging in vivo

doi: 10.1186/s12885-020-07604-8

Figure Lengend Snippet: Ex vivo analysis of multi-color probe uptake. Tumors from animals injected with targeted nanoprobes were excised at 24 h post-injection and imaged on macro- and microscopic imaging platforms. a SWIR and d confocal imaging of Rh-fHoANCs in a 4175TR tumor, b, e FITC-fErANCs in a MCF7 tumor, and c, f 647-fTmANCs in a SKOV3 tumor. Confocal images d, e, f were obtained by measuring the signal from the visible fluorophores loaded into each probe (rhodamine, FITC, Alexa Fluor 647, respectively) Panels b , c , f , g , j , and k each present two representative animals from each experimental group.

Article Snippet: 1 × 10 4175TR cells were inoculated into the left dorsal flank and 5 × 10 6 MCF7 cells were inoculated in the right dorsal flank of 3–4-week-old female athymic nude mice (Taconic Biosciences, Hudson, NY) supplemented with 17β-estradiol pellets releasing estradiol at a rate of 16.66 μg/day.

Techniques: Ex Vivo, Injection, Imaging