cd57 176yb  (fluidigm)

Journal: STAR Protocols

Article Title: Simplified mass cytometry protocol for in-plate staining, barcoding, and cryopreservation of human PBMC samples in clinical trials

doi: 10.1016/j.xpro.2022.101362

Figure Lengend Snippet: Surface antibody master mix, titrated for staining of up to 3 M cells per condition

Article Snippet: Anti-Human CD57 (HCD57)-163Dy—100 Tests (1:200) , Fluidigm , Cat#3163022B.

Techniques: Staining

Journal: STAR Protocols

Article Title: Simplified mass cytometry protocol for in-plate staining, barcoding, and cryopreservation of human PBMC samples in clinical trials

doi: 10.1016/j.xpro.2022.101362

Figure Lengend Snippet:

Article Snippet: Anti-Human CD57 (HCD57)-163Dy—100 Tests (1:200) , Fluidigm , Cat#3163022B.

Techniques: Recombinant, Saline, Staining, Blocking Assay, Isolation, Software, Cell Counting, Cytometry

Journal: STAR Protocols

Article Title: Mass cytometry staining for human bone marrow clinical samples

doi: 10.1016/j.xpro.2022.101163

Figure Lengend Snippet: Surface antibody master mix

Article Snippet: Anti-Human CD57 (HCD57)-172Yb—100 Tests (1:200) , Fluidigm , Cat#3172009B.

Techniques:

Journal: STAR Protocols

Article Title: Mass cytometry staining for human bone marrow clinical samples

doi: 10.1016/j.xpro.2022.101163

Figure Lengend Snippet:

Article Snippet: Anti-Human CD57 (HCD57)-172Yb—100 Tests (1:200) , Fluidigm , Cat#3172009B.

Techniques: Recombinant, Saline, Red Blood Cell Lysis, Staining, Blocking Assay, Antibody Labeling, Software, Cell Counting, Cytometry

Journal: iScience

Article Title: PD-L1 + CD8 + T cells enrichment in lung cancer exerted regulatory function and tumor-promoting tolerance

doi: 10.1016/j.isci.2022.103785

Figure Lengend Snippet:

Article Snippet: anti-human CD57 -176Yb , Fluidigm , Cat# 3176019B.

Techniques: Recombinant, Antibody Labeling, Enzyme-linked Immunosorbent Assay, Sequencing, Software

Journal: iScience

Article Title: PD-L1 + CD8 + T cells enrichment in lung cancer exerted regulatory function and tumor-promoting tolerance

doi: 10.1016/j.isci.2022.103785

Figure Lengend Snippet: PD-L1-expression CD8 + T cells were enriched in lung tumor lesions (A) viSNE analysis of CD3 + T cells, showing the identification of 16 main clusters. Each dot corresponds to a single cell, colored according to the cell cluster. (B) Normalized expression of related markers on CD3 + T cells shown by a viSNE plot. (C) Heatmap of Phenograph clusters of CD3 + T cells. The relative expression levels of markers across cells are shown, sorted by cell types. (D) Bar plots of the frequencies of the clusters from (A) across tissue samples from 10 lung cancer patients (∗p <0.05 and ∗∗p <0.01, paired two-tailed t-test). (E) viSNE analysis of CD8 + T cells, showing the identification of 11 clusters. Each dot corresponds to a single cell, colored according to the cell cluster. (F) Normalized expression of PD1 and PD-L1 on CD8 + cells shown by the viSNE plot in (E). (G) Normalized expression of CD38, CD39, CD103, CD127, CD45RA, TIM3, IFNγ, and Granzyme B in CD8 + T cell clusters shown by the viSNE plot in (E). (H) Bar plots of the frequencies of cluster one of the CD8 viSNE plot across tissue samples for 12 lung tumor patients. (I) Heatmap of the Phenograph clusters 1, five, and seven of CD8 + cells in (E). The relative expression levels of markers across cells are shown. (J) FACS analysis of PD-L1 and CD38 expression in CD8 + T cells from adjacent and tumor tissues (left). Bar plots show the frequencies of CD8 + PD-L1 + CD38 + T cells in different patients (N = 7, ∗p <0.05, paired two-tailed t-test). (K) The ratio of CD4 + CD57 + T cell to CD4 + Treg cluster frequencies and CD8 + CD57 + T cell to PD-L1 + CD8 + cell cluster frequencies across tissue samples (N = 10). ∗p <0.05 and ∗∗p <0.01, paired two-tailed t-test.

Article Snippet: anti-human CD57 -176Yb , Fluidigm , Cat# 3176019B.

Techniques: Expressing, Two Tailed Test

Journal: iScience

Article Title: PD-L1 + CD8 + T cells enrichment in lung cancer exerted regulatory function and tumor-promoting tolerance

doi: 10.1016/j.isci.2022.103785

Figure Lengend Snippet:

Article Snippet: anti-human CD57 -176Yb , Fluidigm , Cat# 3176019B.

Techniques: Recombinant, Antibody Labeling, Enzyme-linked Immunosorbent Assay, Sequencing, Software