3170007b  (fluidigm)

Journal: iScience

Article Title: A nonhuman primate model of vertical sleeve gastrectomy facilitates mechanistic and translational research in human obesity

doi: 10.1016/j.isci.2021.103421

Figure Lengend Snippet:

Article Snippet: CD3 , Fluidigm , Cat#3170007B; Clone: SP34-2.

Techniques: Recombinant, Blocking Assay, Staining, Antibody Labeling, Multiplex Assay, Software

Journal: iScience

Article Title: A nonhuman primate model of vertical sleeve gastrectomy facilitates mechanistic and translational research in human obesity

doi: 10.1016/j.isci.2021.103421

Figure Lengend Snippet: Macrophage phenotypes, CLSs, and T cell infiltration in AT one year after VSG (A and B) Tissue-resident macrophages (CD64 + CD45 + stromal vascular cells) in VAT were significantly suppressed after VSG (n = 3) compared to sham (n = 3) (A), with less CD206 expression (a classic M2 macrophage marker) and less expression of activation markers CD11c, HLA-DR, and CD169 (B). (C–F) (C) CLS density (expressed as Δchange from D0) trended to slightly increased after sham surgery, but was significantly reduced in VAT after VSG suggested reduced inflammation in the VAT. This was VAT-compartment specific, with SAT (D) demonstrating increases in CLSs after both sham surgery and VSG. Similar trends were seen with CD3 + T cells, with a decrease after VSG in VAT (E) but not SAT (F). ∗p < 0.05, sham vs. VSG; #p = 0.05, sham vs. VSG. Data reported as group average ±SEM. See also Figure S3 .

Article Snippet: CD3 , Fluidigm , Cat#3170007B; Clone: SP34-2.

Techniques: Expressing, Marker, Activation Assay

Journal: iScience

Article Title: A nonhuman primate model of vertical sleeve gastrectomy facilitates mechanistic and translational research in human obesity

doi: 10.1016/j.isci.2021.103421

Figure Lengend Snippet: Diversity of myeloid and lymphoid immune cells one month after VSG (A–C) Our short-term study (A) was designed to characterize early immunocyte changes in a group of animals with matched weight loss following VSG to the long-term study (B). VSG promoted M2-like macrophage polarization, increased the number of regulatory T cells, and decreased the number of proliferating T cells in the adipose tissue. Fold-change from baseline in PBLs, SAT, and VAT in the ratio of: (C) M2/M1 macrophages (M1: CD206 + CD11c + , M2: CD206 + CD11c − of CD14 + CD68 + CD3 - lym), M2/M1 in SAT n = 1, all others n = 2. (D) Tregs (FoxP3 + CD4 + of CD3 + lym). (E) t -SNE projection of 26,400 myeloid cells (CD3 − CD20 - /CD45 + lym) sampled equally from PBLs (n = 2), SAT (n = 2), and VAT (n = 2) at D0 and D28 after VSG. (F) 12 individual FlowSOM-identified myeloid populations from the t -SNE projection in (E) as fold change from D0. (G) Heatmap showing mean expression of indicated markers across the 12 FlowSOM myeloid populations. (H) t -SNE projection of 4860 Tregs (CD25 + CD127 - /CD4 + lym) sampled equally from PBLs (n = 2), SAT (n = 2), and VAT (n = 2) at D0 and D28 after VSG. (I) Six individual FlowSOM-identified Treg populations from the t -SNE projects in (H) as fold change from D0. (J) Heatmap showing mean expression of indicated markers across the six FlowSOM Treg populations. Data reported as group average ±SEM. See also , , and , and and .

Article Snippet: CD3 , Fluidigm , Cat#3170007B; Clone: SP34-2.

Techniques: Expressing

Journal: iScience

Article Title: A nonhuman primate model of vertical sleeve gastrectomy facilitates mechanistic and translational research in human obesity

doi: 10.1016/j.isci.2021.103421

Figure Lengend Snippet:

Article Snippet: CD3 , Fluidigm , Cat#3170007B; Clone: SP34-2.

Techniques: Recombinant, Blocking Assay, Staining, Antibody Labeling, Multiplex Assay, Software