3166012b rrid ab 2756424 167er cd38 hit2 standard biotools cat 3167001b rrid ab 2802110 168er mki67 ki 67 standard biotools cat 3168007b  (fluidigm)

Journal: iScience

Article Title: Single-cell transcriptome and crosstalk analysis reveals immune alterations and key pathways in the bone marrow of knee OA patients

doi: 10.1016/j.isci.2024.110827

Figure Lengend Snippet: Detailed cellular crosstalk analysis in BMAC samples (A) Cellular interaction profiling using CellChat. This panel visualizes the top 20 cellular interactions identified within the OA cohort, across both training and validation datasets. The analysis highlights dynamic communication networks, predominantly featuring interactions between mesenchymal stem cells (MSCs) and other critical immune cells such as NK cells, T cells, and hematopoietic stem and progenitor cells (HSPCs). The graphical representation delineates the complexity of intercellular communications, underlining the enriched signaling pathways that potentially influence therapeutic outcomes. (B) The word cloud illustrates the relative prominence of ligand-receptor pairs in the OA and non-OA groups, emphasizing the differential expression of key molecules. Notable ligands such as LAMA4, LAMB1, BMP5, LAMC1, PTPRM, and SEMA4A are exclusively enriched in the OA cohort, suggesting a unique molecular signature that may be pivotal in OA pathogenesis and progression. (C) Comparative analysis of enriched ligand pathways: presented as a bar plot, this panel quantifies and compares the enriched ligand-receptor pathways between OA and non-OA groups. Color-coded for intuitive interpretation (OA in Blue, non-OA in Red), the plot provides a visual summary of the pathway distribution, highlighting the presence of multiple ligand-receptor interactions unique to the OA group. This differential pathway activity could inform targeted therapeutic strategies.

Article Snippet: Anti-Human CD34 (581)-166Er , Fluidigm Corporation , Cat#3166012B.

Techniques: Biomarker Discovery, Protein-Protein interactions, Quantitative Proteomics, Activity Assay

Journal: iScience

Article Title: Single-cell transcriptome and crosstalk analysis reveals immune alterations and key pathways in the bone marrow of knee OA patients

doi: 10.1016/j.isci.2024.110827

Figure Lengend Snippet:

Article Snippet: Anti-Human CD34 (581)-166Er , Fluidigm Corporation , Cat#3166012B.

Techniques: Recombinant, Software

Journal: Cell

Article Title: Complement activation induces excessive T cell cytotoxicity in severe COVID-19

doi: 10.1016/j.cell.2021.12.040

Figure Lengend Snippet:

Article Snippet: CD34 166Er (581) , Fluidigm , Cat# 3166012B; RRID: AB_2756424.

Techniques: Purification, Functional Assay, Blocking Assay, Recombinant, Lysis, Cell Isolation, Staining, Positive Control, Virus, Software, Derivative Assay