ly6c hk1 4 162dy cytof standard biotools 3162014b  (fluidigm)

Journal: STAR Protocols

Article Title: Protocol for phenotyping mouse myeloid and lymphoid cells by mass cytometry

doi: 10.1016/j.xpro.2025.103684

Figure Lengend Snippet: Summary table of markers expressed for each cell type in mouse lungs, spleen and peritoneal lavage

Article Snippet: Anti-mouse Ly6c (HK1.4)-162Dy-100 tests , Standard BioTools , Cat#3162014B; RRID: AB_2922921.

Techniques:

Journal: STAR Protocols

Article Title: Protocol for phenotyping mouse myeloid and lymphoid cells by mass cytometry

doi: 10.1016/j.xpro.2025.103684

Figure Lengend Snippet: Proposed gating strategy for identifying key murine tissue cell populations in both peritoneal lavage and lung tissue Mouse lung cells were isolated and labeled with the panel of 37 markers proposed in this protocol. After cell acquisition using the Helios mass cytometer and data cleaning, cells were identified using a gating strategy on the OMIQ software. The CD45- cells and CD45+ immune cells were first identified. We then identify B cells (CD19 + ) and among them B1 and B2 subpopulations, T cells (CD3 + ) and among them LTCD8+ and LTCD4+ and notably LTreg (Foxp3 + ), Natural killers (NK1.1 + ), neutrophils (Ly6G + ) and eosinophils (CD24 + CD170 + ). The remaining population, called "other myeloid cells", consisted mainly of monocytes, macrophages and dendritic cells (DC). In peritoneal lavage, the two populations of conventional DC, cDC1 and cDC2, were identified among mDC (CD11c + ) based on CD172a and CD209a staining. After exclusion of plasmacytoid DC (pDC) (SiglecH + ), two subpopulations of peritoneal macrophages (PM) (CD68 + F4/80 + ) were identified, the small PM (SPM)(I-A/I-E hi and CCR hi ) and the large PM (LPM). Finally, we identified classical monocytes based on Ly6c hi and CD43 lo staining. In lung tissue, we first identified alveolar macrophages (AM) (CD64 + CD68 + ) and after DC exclusion, we identified interstitial macrophages (IM) subpopulations (CD11b + I-A/I-E + ) and classical monocytes (Ly6c hi and CD43 lo ). Among CD11c + dendritic cells, we next identified plasmacytoid DC (pDC) (SiglecH + ) and the two subpopulations of conventional DC named cDC1 (CD103 + ) and cDC2 (CD172a + ).

Article Snippet: Anti-mouse Ly6c (HK1.4)-162Dy-100 tests , Standard BioTools , Cat#3162014B; RRID: AB_2922921.

Techniques: Isolation, Labeling, Cytometry, Software, Staining

Journal: STAR Protocols

Article Title: Protocol for phenotyping mouse myeloid and lymphoid cells by mass cytometry

doi: 10.1016/j.xpro.2025.103684

Figure Lengend Snippet: Identification of lung plasmacytoid dendritic cells by unsupervised analysis Flowsom cluster analysis of lung monocytes, macrophages and dendritic cells from merged data of five control mice, the cluster 5 representing pDC (A), and UMAP of the SiglecH expression on these lung cells (B). Heatmap of the median expression value of pDC key markers (CD45, CD11c, CD209a, Ly6C, SiglecH) across the 10 metaclusters identified by Flowsom algorithm (C).

Article Snippet: Anti-mouse Ly6c (HK1.4)-162Dy-100 tests , Standard BioTools , Cat#3162014B; RRID: AB_2922921.

Techniques: Control, Expressing

Journal: STAR Protocols

Article Title: Protocol for phenotyping mouse myeloid and lymphoid cells by mass cytometry

doi: 10.1016/j.xpro.2025.103684

Figure Lengend Snippet:

Article Snippet: Anti-mouse Ly6c (HK1.4)-162Dy-100 tests , Standard BioTools , Cat#3162014B; RRID: AB_2922921.

Techniques: Purification, Blocking Assay, Recombinant, Sterility, Staining, Red Blood Cell Lysis, Antibody Labeling, Software, Membrane, Transferring, Spectrophotometry

Journal: Cell Reports Medicine

Article Title: LTA4H improves the tumor microenvironment and prevents HCC progression via targeting the HNRNPA1/LTBP1/TGF-β axis

doi: 10.1016/j.xcrm.2025.102000

Figure Lengend Snippet:

Article Snippet: Ly-6C; Mouse; 162Dy , Fluidigm , 3162014B; RRID: AB_2922921.

Techniques: Phospho-proteomics, Microarray, Recombinant, Lysis, Protease Inhibitor, SYBR Green Assay, Enzyme-linked Immunosorbent Assay, TUNEL Assay, Reverse Transcription, Activity Assay, Immunoprecipitation, Extraction, Purification, In Vitro, Transfection, Sequencing, Mass Cytometry, ChIP-sequencing, Transgenic Assay, Software