7319 1526gd fluidigm 201156a 81 159tb ccr7 g043h7 fluidigm 3159003a 160gd fitc fit22 fluidigm 3160011b il 35 fit22 ls bio ls  (fluidigm)

Journal: Cancer Cell

Article Title: Single-cell analysis defines a pancreatic fibroblast lineage that supports anti-tumor immunity

doi: 10.1016/j.ccell.2021.06.017

Figure Lengend Snippet:

Article Snippet: Anti-FITC 160Gd clone FIT-22 , Fluidigm , 3160011B.

Techniques: Blocking Assay, Virus, Recombinant, Plasmid Preparation, Saline, Lysis, Staining, Reverse Transcription, In Vivo, Electroporation, Mass Cytometry, Conjugation Assay, Illumina Sequencing, Library Quantification, In Vitro, Quantitative RT-PCR, Software, Real-time Polymerase Chain Reaction, Cytometry, Microscopy, Imaging, Spectrophotometry

Journal: Cancer Cell

Article Title: Single-cell analysis defines a pancreatic fibroblast lineage that supports anti-tumor immunity

doi: 10.1016/j.ccell.2021.06.017

Figure Lengend Snippet:

Article Snippet: Anti-FITC 160Gd clone FIT-22 , Fluidigm , 3160011B.

Techniques: Blocking Assay, Virus, Recombinant, Plasmid Preparation, Saline, Lysis, Staining, Reverse Transcription, In Vivo, Electroporation, Mass Cytometry, Conjugation Assay, Illumina Sequencing, Library Quantification, In Vitro, Quantitative RT-PCR, Software, Real-time Polymerase Chain Reaction, Cytometry, Microscopy, Imaging, Spectrophotometry

Journal: Magnetochemistry (Basel, Switzerland)

Article Title: Enrichment and Detection of Antigen-Binding B Cells for Mass Cytometry

doi: 10.3390/magnetochemistry7070092

Figure Lengend Snippet: Schematic for magnetic enrichment of antigen-reactive B cells. 1. Human PBMCs are incubated with properly titrated amounts of biotin and FITC-labeled antigens. Cells are then incubated with a combination of cleavable anti-biotin and anti-FITC magnetic beads. 2. Washed cells are then added to a magnetic column and separated into depleted and enriched fractions. 3. Magnetic microbeads are cleaved off and washed away. 4. Cells are then incubated with an antibody cocktail containing various metal labeled antibodies. 5. Cells are analyzed on a mass cytometer. 6. Data analysis is performed using a combination of manual gating and dimensionality reduction and clustering algorithms.

Article Snippet: 160Gd-anti-FITC antibody , Fluidigm , 3160011B , .

Techniques: Incubation, Labeling, Magnetic Beads, Cytometry

Journal: Magnetochemistry (Basel, Switzerland)

Article Title: Enrichment and Detection of Antigen-Binding B Cells for Mass Cytometry

doi: 10.3390/magnetochemistry7070092

Figure Lengend Snippet: Materials.

Article Snippet: 160Gd-anti-FITC antibody , Fluidigm , 3160011B , .

Techniques: Blocking Assay, Antibody Labeling, Staining

Journal: Magnetochemistry (Basel, Switzerland)

Article Title: Enrichment and Detection of Antigen-Binding B Cells for Mass Cytometry

doi: 10.3390/magnetochemistry7070092

Figure Lengend Snippet: Antibody panel.

Article Snippet: 160Gd-anti-FITC antibody , Fluidigm , 3160011B , .

Techniques: