cd45ra (fluidigm)
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cd45ra
Cd45ra, supplied by fluidigm, used in various techniques. Bioz Stars score: 95/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/3155011b/Anti-Human+CD45RA+(HI100)-155Gd/pmc12864045-17-2-8
Average 95 stars, based on 44 article reviews
Cd45ra, supplied by fluidigm, used in various techniques. Bioz Stars score: 95/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/3155011b/Anti-Human+CD45RA+(HI100)-155Gd/pmc12864045-17-2-8
Average 95 stars, based on 44 article reviews
cd45ra - by Bioz Stars,
2026-09
95/100 stars
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Purification:Article Title: Somatic deficiency of the human E3 ubiquitin ligase CBL in leukocytes impairs B cell but not T cell development and function Article Snippet: 155Gd , CD45RA , HI100 , Article Title: PARP-inhibition reprograms macrophages toward an anti-tumor phenotype. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Olaparib MCE Cat#HY-10162 H2DCFDA (H2-DCF, DCF) ThermoFisher Cat#D399 N-Methylnicotinamide (MNA) FisherScientific Cat#M037425G Mitoquinone (MitoQ10) mesylate Selleck Cat#S8978 Rotenone Agilent Cat#103015-100 Trolox MCE Cat#HY-101445 MitoSOX ThermoFisher Cat#M36008 b-Estradiol Sigma-Aldrich Cat#E8875 Clodronate Liposomes & Control Liposomes (PBS) Liposoma Cat#CP-050-050 Recombinant murine IL-4 Peprotech Cat#214-14 Recombinant murine M-CSF Peprotech Cat#315-02 Recombinant mouse IL-10 Prospec Bio Cat#CYT-497 Recombinant mouse GM-CSF Peprotech Cat#315-03 IVISbrite D-Luciferin Potassium Salt Bioluminescent Substrate (1g) (XenoLight) PerkinElmer Cat#122799 Critical commercial assays QtrackerTM 625 Cell Labeling Kit ThermoFisher Cat#A10198 NAD/NADH Quantitation Colorimetric Kit Biovision Cat#K337 CellTraceTM Violet Cell Proliferation Kit ThermoFisher Cat#C34557 Double stain IHC kit Abcam Cat#ab183285 Opal 7-Color IHC Kit Akoya Cat# NEL811001KT Seahorse XF Glycolysis Stress Test Kit Seahorse Bioscience Cat#103020-100 Seahorse XF Cell Mito Stress Test Kit Seahorse Bioscience Cat#103015-100 Deposited data RNA seq data This paper GEO: GSE210378 Mass Spectrometry data This paper https://doi.org/10.6084/m9.figshare.20640090 https://doi.org/10.6084/m9.figshare.20639979 https://doi.org/10.6084/m9.figshare.20639895 https://doi.org/10.6084/m9.figshare.20640195 EMBL-EBI MetaboLights database https://doi.org/ 10.1093/nar/gkz1019, PMID:31691833 with the identifier MTBLS5928 Code for Akoya data analysis This paper https://github.com/WulfLab/MacrophagM- Quantification.git or https://doi.org/10.5281/ zenodo.7071901 Experimental models: Cell lines K14-GFP This paper N/A iBMPC This paper N/A Experimental models: Organisms/strains Mouse: FVB/129P2Ola This paper N/A Mouse: NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) Jackson Laboratory RRID: IMSR_JAX:005557 Mouse: CB17.Cg-PrkdcscidLystbg-J/Crl (Scid/Beige) Charles River RRID: IMSR_CRL:250 Mouse: 129S-Parp1tm1Zqw/J (Parp1 null) Jackson Laboratory RRID: IMSR_JAX:002779 Mouse: Parp2 knockout Peter Bai Lab N/A Mouse: C57BL6/J Jackson Laboratory RRID: IMSR_JAX:000664 Mouse: C57BL/6-Tg(TcraTcrb)1100Mjb/J (OTI) Jackson Laboratory RRID: IMSR_JAX:003831 Mouse: FVB/NJ (FVB) Jackson Laboratory RRID: IMSR_JAX:001800 Oligonucleotides qPCR primers for Figures 3 and S4, see Table S3 This paper N/A (Continued on next page) Cell Reports 41, 111462, October 11, 2022 e2 Article Title: Protocol for large scale whole blood immune monitoring by mass cytometry and Cyto Quality Pipeline Article Snippet: Anti-Human CD45RA (HI100)-155Gd—100 Tests , Recombinant:Article Title: Somatic deficiency of the human E3 ubiquitin ligase CBL in leukocytes impairs B cell but not T cell development and function Article Snippet: 155Gd , CD45RA , HI100 , Article Title: PARP-inhibition reprograms macrophages toward an anti-tumor phenotype. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Olaparib MCE Cat#HY-10162 H2DCFDA (H2-DCF, DCF) ThermoFisher Cat#D399 N-Methylnicotinamide (MNA) FisherScientific Cat#M037425G Mitoquinone (MitoQ10) mesylate Selleck Cat#S8978 Rotenone Agilent Cat#103015-100 Trolox MCE Cat#HY-101445 MitoSOX ThermoFisher Cat#M36008 b-Estradiol Sigma-Aldrich Cat#E8875 Clodronate Liposomes & Control Liposomes (PBS) Liposoma Cat#CP-050-050 Recombinant murine IL-4 Peprotech Cat#214-14 Recombinant murine M-CSF Peprotech Cat#315-02 Recombinant mouse IL-10 Prospec Bio Cat#CYT-497 Recombinant mouse GM-CSF Peprotech Cat#315-03 IVISbrite D-Luciferin Potassium Salt Bioluminescent Substrate (1g) (XenoLight) PerkinElmer Cat#122799 Critical commercial assays QtrackerTM 625 Cell Labeling Kit ThermoFisher Cat#A10198 NAD/NADH Quantitation Colorimetric Kit Biovision Cat#K337 CellTraceTM Violet Cell Proliferation Kit ThermoFisher Cat#C34557 Double stain IHC kit Abcam Cat#ab183285 Opal 7-Color IHC Kit Akoya Cat# NEL811001KT Seahorse XF Glycolysis Stress Test Kit Seahorse Bioscience Cat#103020-100 Seahorse XF Cell Mito Stress Test Kit Seahorse Bioscience Cat#103015-100 Deposited data RNA seq data This paper GEO: GSE210378 Mass Spectrometry data This paper https://doi.org/10.6084/m9.figshare.20640090 https://doi.org/10.6084/m9.figshare.20639979 https://doi.org/10.6084/m9.figshare.20639895 https://doi.org/10.6084/m9.figshare.20640195 EMBL-EBI MetaboLights database https://doi.org/ 10.1093/nar/gkz1019, PMID:31691833 with the identifier MTBLS5928 Code for Akoya data analysis This paper https://github.com/WulfLab/MacrophagM- Quantification.git or https://doi.org/10.5281/ zenodo.7071901 Experimental models: Cell lines K14-GFP This paper N/A iBMPC This paper N/A Experimental models: Organisms/strains Mouse: FVB/129P2Ola This paper N/A Mouse: NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) Jackson Laboratory RRID: IMSR_JAX:005557 Mouse: CB17.Cg-PrkdcscidLystbg-J/Crl (Scid/Beige) Charles River RRID: IMSR_CRL:250 Mouse: 129S-Parp1tm1Zqw/J (Parp1 null) Jackson Laboratory RRID: IMSR_JAX:002779 Mouse: Parp2 knockout Peter Bai Lab N/A Mouse: C57BL6/J Jackson Laboratory RRID: IMSR_JAX:000664 Mouse: C57BL/6-Tg(TcraTcrb)1100Mjb/J (OTI) Jackson Laboratory RRID: IMSR_JAX:003831 Mouse: FVB/NJ (FVB) Jackson Laboratory RRID: IMSR_JAX:001800 Oligonucleotides qPCR primers for Figures 3 and S4, see Table S3 This paper N/A (Continued on next page) Cell Reports 41, 111462, October 11, 2022 e2 Article Title: Protocol for large scale whole blood immune monitoring by mass cytometry and Cyto Quality Pipeline Article Snippet: Anti-Human CD45RA (HI100)-155Gd—100 Tests , Staining:Article Title: Somatic deficiency of the human E3 ubiquitin ligase CBL in leukocytes impairs B cell but not T cell development and function Article Snippet: 155Gd , CD45RA , HI100 , Article Title: PARP-inhibition reprograms macrophages toward an anti-tumor phenotype. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Olaparib MCE Cat#HY-10162 H2DCFDA (H2-DCF, DCF) ThermoFisher Cat#D399 N-Methylnicotinamide (MNA) FisherScientific Cat#M037425G Mitoquinone (MitoQ10) mesylate Selleck Cat#S8978 Rotenone Agilent Cat#103015-100 Trolox MCE Cat#HY-101445 MitoSOX ThermoFisher Cat#M36008 b-Estradiol Sigma-Aldrich Cat#E8875 Clodronate Liposomes & Control Liposomes (PBS) Liposoma Cat#CP-050-050 Recombinant murine IL-4 Peprotech Cat#214-14 Recombinant murine M-CSF Peprotech Cat#315-02 Recombinant mouse IL-10 Prospec Bio Cat#CYT-497 Recombinant mouse GM-CSF Peprotech Cat#315-03 IVISbrite D-Luciferin Potassium Salt Bioluminescent Substrate (1g) (XenoLight) PerkinElmer Cat#122799 Critical commercial assays QtrackerTM 625 Cell Labeling Kit ThermoFisher Cat#A10198 NAD/NADH Quantitation Colorimetric Kit Biovision Cat#K337 CellTraceTM Violet Cell Proliferation Kit ThermoFisher Cat#C34557 Double stain IHC kit Abcam Cat#ab183285 Opal 7-Color IHC Kit Akoya Cat# NEL811001KT Seahorse XF Glycolysis Stress Test Kit Seahorse Bioscience Cat#103020-100 Seahorse XF Cell Mito Stress Test Kit Seahorse Bioscience Cat#103015-100 Deposited data RNA seq data This paper GEO: GSE210378 Mass Spectrometry data This paper https://doi.org/10.6084/m9.figshare.20640090 https://doi.org/10.6084/m9.figshare.20639979 https://doi.org/10.6084/m9.figshare.20639895 https://doi.org/10.6084/m9.figshare.20640195 EMBL-EBI MetaboLights database https://doi.org/ 10.1093/nar/gkz1019, PMID:31691833 with the identifier MTBLS5928 Code for Akoya data analysis This paper https://github.com/WulfLab/MacrophagM- Quantification.git or https://doi.org/10.5281/ zenodo.7071901 Experimental models: Cell lines K14-GFP This paper N/A iBMPC This paper N/A Experimental models: Organisms/strains Mouse: FVB/129P2Ola This paper N/A Mouse: NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) Jackson Laboratory RRID: IMSR_JAX:005557 Mouse: CB17.Cg-PrkdcscidLystbg-J/Crl (Scid/Beige) Charles River RRID: IMSR_CRL:250 Mouse: 129S-Parp1tm1Zqw/J (Parp1 null) Jackson Laboratory RRID: IMSR_JAX:002779 Mouse: Parp2 knockout Peter Bai Lab N/A Mouse: C57BL6/J Jackson Laboratory RRID: IMSR_JAX:000664 Mouse: C57BL/6-Tg(TcraTcrb)1100Mjb/J (OTI) Jackson Laboratory RRID: IMSR_JAX:003831 Mouse: FVB/NJ (FVB) Jackson Laboratory RRID: IMSR_JAX:001800 Oligonucleotides qPCR primers for Figures 3 and S4, see Table S3 This paper N/A (Continued on next page) Cell Reports 41, 111462, October 11, 2022 e2 Article Title: Protocol for large scale whole blood immune monitoring by mass cytometry and Cyto Quality Pipeline Article Snippet: Anti-Human CD45RA (HI100)-155Gd—100 Tests , Saline:Article Title: Somatic deficiency of the human E3 ubiquitin ligase CBL in leukocytes impairs B cell but not T cell development and function Article Snippet: 155Gd , CD45RA , HI100 , Article Title: PARP-inhibition reprograms macrophages toward an anti-tumor phenotype. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Olaparib MCE Cat#HY-10162 H2DCFDA (H2-DCF, DCF) ThermoFisher Cat#D399 N-Methylnicotinamide (MNA) FisherScientific Cat#M037425G Mitoquinone (MitoQ10) mesylate Selleck Cat#S8978 Rotenone Agilent Cat#103015-100 Trolox MCE Cat#HY-101445 MitoSOX ThermoFisher Cat#M36008 b-Estradiol Sigma-Aldrich Cat#E8875 Clodronate Liposomes & Control Liposomes (PBS) Liposoma Cat#CP-050-050 Recombinant murine IL-4 Peprotech Cat#214-14 Recombinant murine M-CSF Peprotech Cat#315-02 Recombinant mouse IL-10 Prospec Bio Cat#CYT-497 Recombinant mouse GM-CSF Peprotech Cat#315-03 IVISbrite D-Luciferin Potassium Salt Bioluminescent Substrate (1g) (XenoLight) PerkinElmer Cat#122799 Critical commercial assays QtrackerTM 625 Cell Labeling Kit ThermoFisher Cat#A10198 NAD/NADH Quantitation Colorimetric Kit Biovision Cat#K337 CellTraceTM Violet Cell Proliferation Kit ThermoFisher Cat#C34557 Double stain IHC kit Abcam Cat#ab183285 Opal 7-Color IHC Kit Akoya Cat# NEL811001KT Seahorse XF Glycolysis Stress Test Kit Seahorse Bioscience Cat#103020-100 Seahorse XF Cell Mito Stress Test Kit Seahorse Bioscience Cat#103015-100 Deposited data RNA seq data This paper GEO: GSE210378 Mass Spectrometry data This paper https://doi.org/10.6084/m9.figshare.20640090 https://doi.org/10.6084/m9.figshare.20639979 https://doi.org/10.6084/m9.figshare.20639895 https://doi.org/10.6084/m9.figshare.20640195 EMBL-EBI MetaboLights database https://doi.org/ 10.1093/nar/gkz1019, PMID:31691833 with the identifier MTBLS5928 Code for Akoya data analysis This paper https://github.com/WulfLab/MacrophagM- Quantification.git or https://doi.org/10.5281/ zenodo.7071901 Experimental models: Cell lines K14-GFP This paper N/A iBMPC This paper N/A Experimental models: Organisms/strains Mouse: FVB/129P2Ola This paper N/A Mouse: NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) Jackson Laboratory RRID: IMSR_JAX:005557 Mouse: CB17.Cg-PrkdcscidLystbg-J/Crl (Scid/Beige) Charles River RRID: IMSR_CRL:250 Mouse: 129S-Parp1tm1Zqw/J (Parp1 null) Jackson Laboratory RRID: IMSR_JAX:002779 Mouse: Parp2 knockout Peter Bai Lab N/A Mouse: C57BL6/J Jackson Laboratory RRID: IMSR_JAX:000664 Mouse: C57BL/6-Tg(TcraTcrb)1100Mjb/J (OTI) Jackson Laboratory RRID: IMSR_JAX:003831 Mouse: FVB/NJ (FVB) Jackson Laboratory RRID: IMSR_JAX:001800 Oligonucleotides qPCR primers for Figures 3 and S4, see Table S3 This paper N/A (Continued on next page) Cell Reports 41, 111462, October 11, 2022 e2 Article Title: Protocol for large scale whole blood immune monitoring by mass cytometry and Cyto Quality Pipeline Article Snippet: Anti-Human CD45RA (HI100)-155Gd—100 Tests , Antibody Labeling:Article Title: Somatic deficiency of the human E3 ubiquitin ligase CBL in leukocytes impairs B cell but not T cell development and function Article Snippet: 155Gd , CD45RA , HI100 , Article Title: PARP-inhibition reprograms macrophages toward an anti-tumor phenotype. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Olaparib MCE Cat#HY-10162 H2DCFDA (H2-DCF, DCF) ThermoFisher Cat#D399 N-Methylnicotinamide (MNA) FisherScientific Cat#M037425G Mitoquinone (MitoQ10) mesylate Selleck Cat#S8978 Rotenone Agilent Cat#103015-100 Trolox MCE Cat#HY-101445 MitoSOX ThermoFisher Cat#M36008 b-Estradiol Sigma-Aldrich Cat#E8875 Clodronate Liposomes & Control Liposomes (PBS) Liposoma Cat#CP-050-050 Recombinant murine IL-4 Peprotech Cat#214-14 Recombinant murine M-CSF Peprotech Cat#315-02 Recombinant mouse IL-10 Prospec Bio Cat#CYT-497 Recombinant mouse GM-CSF Peprotech Cat#315-03 IVISbrite D-Luciferin Potassium Salt Bioluminescent Substrate (1g) (XenoLight) PerkinElmer Cat#122799 Critical commercial assays QtrackerTM 625 Cell Labeling Kit ThermoFisher Cat#A10198 NAD/NADH Quantitation Colorimetric Kit Biovision Cat#K337 CellTraceTM Violet Cell Proliferation Kit ThermoFisher Cat#C34557 Double stain IHC kit Abcam Cat#ab183285 Opal 7-Color IHC Kit Akoya Cat# NEL811001KT Seahorse XF Glycolysis Stress Test Kit Seahorse Bioscience Cat#103020-100 Seahorse XF Cell Mito Stress Test Kit Seahorse Bioscience Cat#103015-100 Deposited data RNA seq data This paper GEO: GSE210378 Mass Spectrometry data This paper https://doi.org/10.6084/m9.figshare.20640090 https://doi.org/10.6084/m9.figshare.20639979 https://doi.org/10.6084/m9.figshare.20639895 https://doi.org/10.6084/m9.figshare.20640195 EMBL-EBI MetaboLights database https://doi.org/ 10.1093/nar/gkz1019, PMID:31691833 with the identifier MTBLS5928 Code for Akoya data analysis This paper https://github.com/WulfLab/MacrophagM- Quantification.git or https://doi.org/10.5281/ zenodo.7071901 Experimental models: Cell lines K14-GFP This paper N/A iBMPC This paper N/A Experimental models: Organisms/strains Mouse: FVB/129P2Ola This paper N/A Mouse: NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) Jackson Laboratory RRID: IMSR_JAX:005557 Mouse: CB17.Cg-PrkdcscidLystbg-J/Crl (Scid/Beige) Charles River RRID: IMSR_CRL:250 Mouse: 129S-Parp1tm1Zqw/J (Parp1 null) Jackson Laboratory RRID: IMSR_JAX:002779 Mouse: Parp2 knockout Peter Bai Lab N/A Mouse: C57BL6/J Jackson Laboratory RRID: IMSR_JAX:000664 Mouse: C57BL/6-Tg(TcraTcrb)1100Mjb/J (OTI) Jackson Laboratory RRID: IMSR_JAX:003831 Mouse: FVB/NJ (FVB) Jackson Laboratory RRID: IMSR_JAX:001800 Oligonucleotides qPCR primers for Figures 3 and S4, see Table S3 This paper N/A (Continued on next page) Cell Reports 41, 111462, October 11, 2022 e2 Article Title: Protocol for large scale whole blood immune monitoring by mass cytometry and Cyto Quality Pipeline Article Snippet: Anti-Human CD45RA (HI100)-155Gd—100 Tests , Software:Article Title: Somatic deficiency of the human E3 ubiquitin ligase CBL in leukocytes impairs B cell but not T cell development and function Article Snippet: 155Gd , CD45RA , HI100 , Article Title: PARP-inhibition reprograms macrophages toward an anti-tumor phenotype. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Olaparib MCE Cat#HY-10162 H2DCFDA (H2-DCF, DCF) ThermoFisher Cat#D399 N-Methylnicotinamide (MNA) FisherScientific Cat#M037425G Mitoquinone (MitoQ10) mesylate Selleck Cat#S8978 Rotenone Agilent Cat#103015-100 Trolox MCE Cat#HY-101445 MitoSOX ThermoFisher Cat#M36008 b-Estradiol Sigma-Aldrich Cat#E8875 Clodronate Liposomes & Control Liposomes (PBS) Liposoma Cat#CP-050-050 Recombinant murine IL-4 Peprotech Cat#214-14 Recombinant murine M-CSF Peprotech Cat#315-02 Recombinant mouse IL-10 Prospec Bio Cat#CYT-497 Recombinant mouse GM-CSF Peprotech Cat#315-03 IVISbrite D-Luciferin Potassium Salt Bioluminescent Substrate (1g) (XenoLight) PerkinElmer Cat#122799 Critical commercial assays QtrackerTM 625 Cell Labeling Kit ThermoFisher Cat#A10198 NAD/NADH Quantitation Colorimetric Kit Biovision Cat#K337 CellTraceTM Violet Cell Proliferation Kit ThermoFisher Cat#C34557 Double stain IHC kit Abcam Cat#ab183285 Opal 7-Color IHC Kit Akoya Cat# NEL811001KT Seahorse XF Glycolysis Stress Test Kit Seahorse Bioscience Cat#103020-100 Seahorse XF Cell Mito Stress Test Kit Seahorse Bioscience Cat#103015-100 Deposited data RNA seq data This paper GEO: GSE210378 Mass Spectrometry data This paper https://doi.org/10.6084/m9.figshare.20640090 https://doi.org/10.6084/m9.figshare.20639979 https://doi.org/10.6084/m9.figshare.20639895 https://doi.org/10.6084/m9.figshare.20640195 EMBL-EBI MetaboLights database https://doi.org/ 10.1093/nar/gkz1019, PMID:31691833 with the identifier MTBLS5928 Code for Akoya data analysis This paper https://github.com/WulfLab/MacrophagM- Quantification.git or https://doi.org/10.5281/ zenodo.7071901 Experimental models: Cell lines K14-GFP This paper N/A iBMPC This paper N/A Experimental models: Organisms/strains Mouse: FVB/129P2Ola This paper N/A Mouse: NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) Jackson Laboratory RRID: IMSR_JAX:005557 Mouse: CB17.Cg-PrkdcscidLystbg-J/Crl (Scid/Beige) Charles River RRID: IMSR_CRL:250 Mouse: 129S-Parp1tm1Zqw/J (Parp1 null) Jackson Laboratory RRID: IMSR_JAX:002779 Mouse: Parp2 knockout Peter Bai Lab N/A Mouse: C57BL6/J Jackson Laboratory RRID: IMSR_JAX:000664 Mouse: C57BL/6-Tg(TcraTcrb)1100Mjb/J (OTI) Jackson Laboratory RRID: IMSR_JAX:003831 Mouse: FVB/NJ (FVB) Jackson Laboratory RRID: IMSR_JAX:001800 Oligonucleotides qPCR primers for Figures 3 and S4, see Table S3 This paper N/A (Continued on next page) Cell Reports 41, 111462, October 11, 2022 e2 Article Title: Protocol for large scale whole blood immune monitoring by mass cytometry and Cyto Quality Pipeline Article Snippet: Anti-Human CD45RA (HI100)-155Gd—100 Tests , Cell Counting:Article Title: Somatic deficiency of the human E3 ubiquitin ligase CBL in leukocytes impairs B cell but not T cell development and function Article Snippet: 155Gd , CD45RA , HI100 , Article Title: PARP-inhibition reprograms macrophages toward an anti-tumor phenotype. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Olaparib MCE Cat#HY-10162 H2DCFDA (H2-DCF, DCF) ThermoFisher Cat#D399 N-Methylnicotinamide (MNA) FisherScientific Cat#M037425G Mitoquinone (MitoQ10) mesylate Selleck Cat#S8978 Rotenone Agilent Cat#103015-100 Trolox MCE Cat#HY-101445 MitoSOX ThermoFisher Cat#M36008 b-Estradiol Sigma-Aldrich Cat#E8875 Clodronate Liposomes & Control Liposomes (PBS) Liposoma Cat#CP-050-050 Recombinant murine IL-4 Peprotech Cat#214-14 Recombinant murine M-CSF Peprotech Cat#315-02 Recombinant mouse IL-10 Prospec Bio Cat#CYT-497 Recombinant mouse GM-CSF Peprotech Cat#315-03 IVISbrite D-Luciferin Potassium Salt Bioluminescent Substrate (1g) (XenoLight) PerkinElmer Cat#122799 Critical commercial assays QtrackerTM 625 Cell Labeling Kit ThermoFisher Cat#A10198 NAD/NADH Quantitation Colorimetric Kit Biovision Cat#K337 CellTraceTM Violet Cell Proliferation Kit ThermoFisher Cat#C34557 Double stain IHC kit Abcam Cat#ab183285 Opal 7-Color IHC Kit Akoya Cat# NEL811001KT Seahorse XF Glycolysis Stress Test Kit Seahorse Bioscience Cat#103020-100 Seahorse XF Cell Mito Stress Test Kit Seahorse Bioscience Cat#103015-100 Deposited data RNA seq data This paper GEO: GSE210378 Mass Spectrometry data This paper https://doi.org/10.6084/m9.figshare.20640090 https://doi.org/10.6084/m9.figshare.20639979 https://doi.org/10.6084/m9.figshare.20639895 https://doi.org/10.6084/m9.figshare.20640195 EMBL-EBI MetaboLights database https://doi.org/ 10.1093/nar/gkz1019, PMID:31691833 with the identifier MTBLS5928 Code for Akoya data analysis This paper https://github.com/WulfLab/MacrophagM- Quantification.git or https://doi.org/10.5281/ zenodo.7071901 Experimental models: Cell lines K14-GFP This paper N/A iBMPC This paper N/A Experimental models: Organisms/strains Mouse: FVB/129P2Ola This paper N/A Mouse: NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) Jackson Laboratory RRID: IMSR_JAX:005557 Mouse: CB17.Cg-PrkdcscidLystbg-J/Crl (Scid/Beige) Charles River RRID: IMSR_CRL:250 Mouse: 129S-Parp1tm1Zqw/J (Parp1 null) Jackson Laboratory RRID: IMSR_JAX:002779 Mouse: Parp2 knockout Peter Bai Lab N/A Mouse: C57BL6/J Jackson Laboratory RRID: IMSR_JAX:000664 Mouse: C57BL/6-Tg(TcraTcrb)1100Mjb/J (OTI) Jackson Laboratory RRID: IMSR_JAX:003831 Mouse: FVB/NJ (FVB) Jackson Laboratory RRID: IMSR_JAX:001800 Oligonucleotides qPCR primers for Figures 3 and S4, see Table S3 This paper N/A (Continued on next page) Cell Reports 41, 111462, October 11, 2022 e2 Article Title: Protocol for large scale whole blood immune monitoring by mass cytometry and Cyto Quality Pipeline Article Snippet: Anti-Human CD45RA (HI100)-155Gd—100 Tests , Control:Article Title: Somatic deficiency of the human E3 ubiquitin ligase CBL in leukocytes impairs B cell but not T cell development and function Article Snippet: 155Gd , CD45RA , HI100 , Article Title: PARP-inhibition reprograms macrophages toward an anti-tumor phenotype. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Olaparib MCE Cat#HY-10162 H2DCFDA (H2-DCF, DCF) ThermoFisher Cat#D399 N-Methylnicotinamide (MNA) FisherScientific Cat#M037425G Mitoquinone (MitoQ10) mesylate Selleck Cat#S8978 Rotenone Agilent Cat#103015-100 Trolox MCE Cat#HY-101445 MitoSOX ThermoFisher Cat#M36008 b-Estradiol Sigma-Aldrich Cat#E8875 Clodronate Liposomes & Control Liposomes (PBS) Liposoma Cat#CP-050-050 Recombinant murine IL-4 Peprotech Cat#214-14 Recombinant murine M-CSF Peprotech Cat#315-02 Recombinant mouse IL-10 Prospec Bio Cat#CYT-497 Recombinant mouse GM-CSF Peprotech Cat#315-03 IVISbrite D-Luciferin Potassium Salt Bioluminescent Substrate (1g) (XenoLight) PerkinElmer Cat#122799 Critical commercial assays QtrackerTM 625 Cell Labeling Kit ThermoFisher Cat#A10198 NAD/NADH Quantitation Colorimetric Kit Biovision Cat#K337 CellTraceTM Violet Cell Proliferation Kit ThermoFisher Cat#C34557 Double stain IHC kit Abcam Cat#ab183285 Opal 7-Color IHC Kit Akoya Cat# NEL811001KT Seahorse XF Glycolysis Stress Test Kit Seahorse Bioscience Cat#103020-100 Seahorse XF Cell Mito Stress Test Kit Seahorse Bioscience Cat#103015-100 Deposited data RNA seq data This paper GEO: GSE210378 Mass Spectrometry data This paper https://doi.org/10.6084/m9.figshare.20640090 https://doi.org/10.6084/m9.figshare.20639979 https://doi.org/10.6084/m9.figshare.20639895 https://doi.org/10.6084/m9.figshare.20640195 EMBL-EBI MetaboLights database https://doi.org/ 10.1093/nar/gkz1019, PMID:31691833 with the identifier MTBLS5928 Code for Akoya data analysis This paper https://github.com/WulfLab/MacrophagM- Quantification.git or https://doi.org/10.5281/ zenodo.7071901 Experimental models: Cell lines K14-GFP This paper N/A iBMPC This paper N/A Experimental models: Organisms/strains Mouse: FVB/129P2Ola This paper N/A Mouse: NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) Jackson Laboratory RRID: IMSR_JAX:005557 Mouse: CB17.Cg-PrkdcscidLystbg-J/Crl (Scid/Beige) Charles River RRID: IMSR_CRL:250 Mouse: 129S-Parp1tm1Zqw/J (Parp1 null) Jackson Laboratory RRID: IMSR_JAX:002779 Mouse: Parp2 knockout Peter Bai Lab N/A Mouse: C57BL6/J Jackson Laboratory RRID: IMSR_JAX:000664 Mouse: C57BL/6-Tg(TcraTcrb)1100Mjb/J (OTI) Jackson Laboratory RRID: IMSR_JAX:003831 Mouse: FVB/NJ (FVB) Jackson Laboratory RRID: IMSR_JAX:001800 Oligonucleotides qPCR primers for Figures 3 and S4, see Table S3 This paper N/A (Continued on next page) Cell Reports 41, 111462, October 11, 2022 e2 Article Title: Protocol for large scale whole blood immune monitoring by mass cytometry and Cyto Quality Pipeline Article Snippet: Anti-Human CD45RA (HI100)-155Gd—100 Tests , Immunopeptidomics:Article Title: Somatic deficiency of the human E3 ubiquitin ligase CBL in leukocytes impairs B cell but not T cell development and function Article Snippet: 155Gd , CD45RA , HI100 , Article Title: PARP-inhibition reprograms macrophages toward an anti-tumor phenotype. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Olaparib MCE Cat#HY-10162 H2DCFDA (H2-DCF, DCF) ThermoFisher Cat#D399 N-Methylnicotinamide (MNA) FisherScientific Cat#M037425G Mitoquinone (MitoQ10) mesylate Selleck Cat#S8978 Rotenone Agilent Cat#103015-100 Trolox MCE Cat#HY-101445 MitoSOX ThermoFisher Cat#M36008 b-Estradiol Sigma-Aldrich Cat#E8875 Clodronate Liposomes & Control Liposomes (PBS) Liposoma Cat#CP-050-050 Recombinant murine IL-4 Peprotech Cat#214-14 Recombinant murine M-CSF Peprotech Cat#315-02 Recombinant mouse IL-10 Prospec Bio Cat#CYT-497 Recombinant mouse GM-CSF Peprotech Cat#315-03 IVISbrite D-Luciferin Potassium Salt Bioluminescent Substrate (1g) (XenoLight) PerkinElmer Cat#122799 Critical commercial assays QtrackerTM 625 Cell Labeling Kit ThermoFisher Cat#A10198 NAD/NADH Quantitation Colorimetric Kit Biovision Cat#K337 CellTraceTM Violet Cell Proliferation Kit ThermoFisher Cat#C34557 Double stain IHC kit Abcam Cat#ab183285 Opal 7-Color IHC Kit Akoya Cat# NEL811001KT Seahorse XF Glycolysis Stress Test Kit Seahorse Bioscience Cat#103020-100 Seahorse XF Cell Mito Stress Test Kit Seahorse Bioscience Cat#103015-100 Deposited data RNA seq data This paper GEO: GSE210378 Mass Spectrometry data This paper https://doi.org/10.6084/m9.figshare.20640090 https://doi.org/10.6084/m9.figshare.20639979 https://doi.org/10.6084/m9.figshare.20639895 https://doi.org/10.6084/m9.figshare.20640195 EMBL-EBI MetaboLights database https://doi.org/ 10.1093/nar/gkz1019, PMID:31691833 with the identifier MTBLS5928 Code for Akoya data analysis This paper https://github.com/WulfLab/MacrophagM- Quantification.git or https://doi.org/10.5281/ zenodo.7071901 Experimental models: Cell lines K14-GFP This paper N/A iBMPC This paper N/A Experimental models: Organisms/strains Mouse: FVB/129P2Ola This paper N/A Mouse: NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) Jackson Laboratory RRID: IMSR_JAX:005557 Mouse: CB17.Cg-PrkdcscidLystbg-J/Crl (Scid/Beige) Charles River RRID: IMSR_CRL:250 Mouse: 129S-Parp1tm1Zqw/J (Parp1 null) Jackson Laboratory RRID: IMSR_JAX:002779 Mouse: Parp2 knockout Peter Bai Lab N/A Mouse: C57BL6/J Jackson Laboratory RRID: IMSR_JAX:000664 Mouse: C57BL/6-Tg(TcraTcrb)1100Mjb/J (OTI) Jackson Laboratory RRID: IMSR_JAX:003831 Mouse: FVB/NJ (FVB) Jackson Laboratory RRID: IMSR_JAX:001800 Oligonucleotides qPCR primers for Figures 3 and S4, see Table S3 This paper N/A (Continued on next page) Cell Reports 41, 111462, October 11, 2022 e2 Article Title: Protocol for large scale whole blood immune monitoring by mass cytometry and Cyto Quality Pipeline Article Snippet: Anti-Human CD45RA (HI100)-155Gd—100 Tests , |
