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anti human tigit mbsa43  (fluidigm)


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    Structured Review

    fluidigm anti human tigit mbsa43
    Anti Human Tigit Mbsa43, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/3153019b/Anti-Human+TIGIT+(MBSA43)-153Eu/pmc13006393-80-0-5
    Average 93 stars, based on 23 article reviews
    anti human tigit mbsa43 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Purification:

    Article Title: Early IFN-α signatures and persistent dysfunction are distinguishing features of NK cells in severe COVID-19.
    Article Snippet: .. REAGENT or RESOURCES SOURCE IDENTIFIER RANK purified (80704) R&D Systems Cat# MAB683; RRID:AB_2205330 RANKL APC Miltenyi Biotec Cat# 130-098-511; RRID:AB_2656691 SARS Cov-2 Spike specific nanobody AF488 https://doi.org/10.1126/ science.abe6230 N/A SARS-Cov-2 Nucleocapsid Sinobiological SIN-40588-V08B-100 Siglec 8 164Dy (7C9) Fluidigm Cat# 3164017B Siglec8 PE/Cy7 (7C9) Biolegend Cat# 347112; RRID:AB_2629720 Streptavidin BV786 Biolegend Cat# 405249 TBET BV711 (16893) BD Cat# 563320; RRID: AB_2738136 TCR a/b viogreen (REA652) Miltenyi Cat# 130-119-709; RRID: AB_2751815 TCRa/b FITC (IP26) Biolegend Cat# 306706; RRID: AB_314644 TCRgd purified (11F2) Miltenyi Biotec Produced at request TCRy/d FITC (B1) Biolegend Cat# 331208; RRID: AB_1575108 TIGIT 153Eu (MBSA43) Fluidigm Cat# 3153019B; RRID:AB_2756419 TIGIT Dazzle (A15153G) Biolegend Cat# 372716; RRID: AB_2632931 Tim-3 Fitc (F38-2E2) eBioscience Cat# 11-3109-42; RRID: AB_2572488 TNFa Biolegend Cat# 502805; RRID: AB_2814397 TNF-a BV785 (FN50) BD Cat# 502948; RRID: AB_2565858 Chemicals, peptides, and recombinant proteins BD Horizon Brilliant Stain Buffer Becton Dickinson Cat# 563794 RBC lysis buffer 10X Biolegend Cat# 420301 Pierce 16% Formaldehyde (w/v), Methanol-free Thermo Fisher Cat# 28908 Fetal Bovine Serum PAN Biotec Cat# 3302 Stain Buffer (FBS) Becton Dickinson Cat# 554656 Pancoll human, Density: 1.077 g/ml Pan Biotech Cat# P04-601000 FcR Blocking Reagent, human Miltenyi Cat# 130-059-901 Cell-ID Intercalator-Ir Fluidigm Cat# 201192A Permeabilization buffer 10X eBioscience Cat# 00-8333-56 Maxpar PBS Fluidigm Cat# 201058 Maxpar Cell Staining buffer Fluidigm Cat# 201068 Maxpar X8 Multimetal Labeling Kit Fluidigm Cat# 201300 Proteomic stabilizer Smart Tube Inc. Cat# PROT1 KAPA HiFi HotStart Ready Mix Roche Cat# KK2601 Human Tru Stain FcX Biolegend Cat# 422301 SPRIselect Beckmann Coulter Cat# B23318 MagniSort Negative Selection Beads Thermo Fisher Cat# MSNB-6002-74 Lysercell WDF Sysmex Cat# AL-337-564 Fluorocell WDF Sysmex Cat# CV-377-552 IL2(IS) Milenyi Cat# 130-097-748 IFNa Milenyi Cat# 130-095-066 IL10 Immunotools Cat# 11340103 IL6 Immunotools Cat# 11340064 Amphiregulin PeproTech Cat# 100-55B TNFa Immunotools Cat# 11343015 Human IFN-g1b premium grade Miltenyi Biotec Cat# 130-096-481 Antibiotic-Antimyotic GIBCO Life Cat# 15240-062 Human Serum AB Plasma Sigma Cat# H3667-100ml Fetal bovine serum low in endotoxin A.H. Sigma Aldrich Cat# F7524-500ml (Continued on next page) Immunity 54, 2650–2669.e1–e14, November 9, 2021 e4 .. REAGENT or RESOURCES SOURCE IDENTIFIER RANK purified (80704) R&D Systems Cat# MAB683; RRID:AB_2205330 RANKL APC Miltenyi Biotec Cat# 130-098-511; RRID:AB_2656691 SARS Cov-2 Spike specific nanobody AF488 https://doi.org/10.1126/ science.abe6230 N/A SARS-Cov-2 Nucleocapsid Sinobiological SIN-40588-V08B-100 Siglec 8 164Dy (7C9) Fluidigm Cat# 3164017B Siglec8 PE/Cy7 (7C9) Biolegend Cat# 347112; RRID:AB_2629720 Streptavidin BV786 Biolegend Cat# 405249 TBET BV711 (16893) BD Cat# 563320; RRID: AB_2738136 TCR a/b viogreen (REA652) Miltenyi Cat# 130-119-709; RRID: AB_2751815 TCRa/b FITC (IP26) Biolegend Cat# 306706; RRID: AB_314644 TCRgd purified (11F2) Miltenyi Biotec Produced at request TCRy/d FITC (B1) Biolegend Cat# 331208; RRID: AB_1575108 TIGIT 153Eu (MBSA43) Fluidigm Cat# 3153019B; RRID:AB_2756419 TIGIT Dazzle (A15153G) Biolegend Cat# 372716; RRID: AB_2632931 Tim-3 Fitc (F38-2E2) eBioscience Cat# 11-3109-42; RRID: AB_2572488 TNFa Biolegend Cat# 502805; RRID: AB_2814397 TNF-a BV785 (FN50) BD Cat# 502948; RRID: AB_2565858 Chemicals, peptides, and recombinant proteins BD Horizon Brilliant Stain Buffer Becton Dickinson Cat# 563794 RBC lysis buffer 10X Biolegend Cat# 420301 Pierce 16% Formaldehyde (w/v), Methanol-free Thermo Fisher Cat# 28908 Fetal Bovine Serum PAN Biotec Cat# 3302 Stain Buffer (FBS) Becton Dickinson Cat# 554656 Pancoll human, Density: 1.077 g/ml Pan Biotech Cat# P04-601000 FcR Blocking Reagent, human Miltenyi Cat# 130-059-901 Cell-ID Intercalator-Ir Fluidigm Cat# 201192A Permeabilization buffer 10X eBioscience Cat# 00-8333-56 Maxpar PBS Fluidigm Cat# 201058 Maxpar Cell Staining buffer Fluidigm Cat# 201068 Maxpar X8 Multimetal Labeling Kit Fluidigm Cat# 201300 Proteomic stabilizer Smart Tube Inc. Cat# PROT1 KAPA HiFi HotStart Ready Mix Roche Cat# KK2601 Human Tru Stain FcX Biolegend Cat# 422301 SPRIselect Beckmann Coulter Cat# B23318 MagniSort Negative Selection Beads Thermo Fisher Cat# MSNB-6002-74 Lysercell WDF Sysmex Cat# AL-337-564 Fluorocell WDF Sysmex Cat# CV-377-552 IL2(IS) Milenyi Cat# 130-097-748 IFNa Milenyi Cat# 130-095-066 IL10 Immunotools Cat# 11340103 IL6 Immunotools Cat# 11340064 Amphiregulin PeproTech Cat# 100-55B TNFa Immunotools Cat# 11343015 Human IFN-g1b premium grade Miltenyi Biotec Cat# 130-096-481 Antibiotic-Antimyotic GIBCO Life Cat# 15240-062 Human Serum AB Plasma Sigma Cat# H3667-100ml Fetal bovine serum low in endotoxin A.H. Sigma Aldrich Cat# F7524-500ml (Continued on next page) Immunity 54, 2650–2669.e1–e14, November 9, 2021 e4

    Produced:

    Article Title: Early IFN-α signatures and persistent dysfunction are distinguishing features of NK cells in severe COVID-19.
    Article Snippet: .. REAGENT or RESOURCES SOURCE IDENTIFIER RANK purified (80704) R&D Systems Cat# MAB683; RRID:AB_2205330 RANKL APC Miltenyi Biotec Cat# 130-098-511; RRID:AB_2656691 SARS Cov-2 Spike specific nanobody AF488 https://doi.org/10.1126/ science.abe6230 N/A SARS-Cov-2 Nucleocapsid Sinobiological SIN-40588-V08B-100 Siglec 8 164Dy (7C9) Fluidigm Cat# 3164017B Siglec8 PE/Cy7 (7C9) Biolegend Cat# 347112; RRID:AB_2629720 Streptavidin BV786 Biolegend Cat# 405249 TBET BV711 (16893) BD Cat# 563320; RRID: AB_2738136 TCR a/b viogreen (REA652) Miltenyi Cat# 130-119-709; RRID: AB_2751815 TCRa/b FITC (IP26) Biolegend Cat# 306706; RRID: AB_314644 TCRgd purified (11F2) Miltenyi Biotec Produced at request TCRy/d FITC (B1) Biolegend Cat# 331208; RRID: AB_1575108 TIGIT 153Eu (MBSA43) Fluidigm Cat# 3153019B; RRID:AB_2756419 TIGIT Dazzle (A15153G) Biolegend Cat# 372716; RRID: AB_2632931 Tim-3 Fitc (F38-2E2) eBioscience Cat# 11-3109-42; RRID: AB_2572488 TNFa Biolegend Cat# 502805; RRID: AB_2814397 TNF-a BV785 (FN50) BD Cat# 502948; RRID: AB_2565858 Chemicals, peptides, and recombinant proteins BD Horizon Brilliant Stain Buffer Becton Dickinson Cat# 563794 RBC lysis buffer 10X Biolegend Cat# 420301 Pierce 16% Formaldehyde (w/v), Methanol-free Thermo Fisher Cat# 28908 Fetal Bovine Serum PAN Biotec Cat# 3302 Stain Buffer (FBS) Becton Dickinson Cat# 554656 Pancoll human, Density: 1.077 g/ml Pan Biotech Cat# P04-601000 FcR Blocking Reagent, human Miltenyi Cat# 130-059-901 Cell-ID Intercalator-Ir Fluidigm Cat# 201192A Permeabilization buffer 10X eBioscience Cat# 00-8333-56 Maxpar PBS Fluidigm Cat# 201058 Maxpar Cell Staining buffer Fluidigm Cat# 201068 Maxpar X8 Multimetal Labeling Kit Fluidigm Cat# 201300 Proteomic stabilizer Smart Tube Inc. Cat# PROT1 KAPA HiFi HotStart Ready Mix Roche Cat# KK2601 Human Tru Stain FcX Biolegend Cat# 422301 SPRIselect Beckmann Coulter Cat# B23318 MagniSort Negative Selection Beads Thermo Fisher Cat# MSNB-6002-74 Lysercell WDF Sysmex Cat# AL-337-564 Fluorocell WDF Sysmex Cat# CV-377-552 IL2(IS) Milenyi Cat# 130-097-748 IFNa Milenyi Cat# 130-095-066 IL10 Immunotools Cat# 11340103 IL6 Immunotools Cat# 11340064 Amphiregulin PeproTech Cat# 100-55B TNFa Immunotools Cat# 11343015 Human IFN-g1b premium grade Miltenyi Biotec Cat# 130-096-481 Antibiotic-Antimyotic GIBCO Life Cat# 15240-062 Human Serum AB Plasma Sigma Cat# H3667-100ml Fetal bovine serum low in endotoxin A.H. Sigma Aldrich Cat# F7524-500ml (Continued on next page) Immunity 54, 2650–2669.e1–e14, November 9, 2021 e4 .. REAGENT or RESOURCES SOURCE IDENTIFIER RANK purified (80704) R&D Systems Cat# MAB683; RRID:AB_2205330 RANKL APC Miltenyi Biotec Cat# 130-098-511; RRID:AB_2656691 SARS Cov-2 Spike specific nanobody AF488 https://doi.org/10.1126/ science.abe6230 N/A SARS-Cov-2 Nucleocapsid Sinobiological SIN-40588-V08B-100 Siglec 8 164Dy (7C9) Fluidigm Cat# 3164017B Siglec8 PE/Cy7 (7C9) Biolegend Cat# 347112; RRID:AB_2629720 Streptavidin BV786 Biolegend Cat# 405249 TBET BV711 (16893) BD Cat# 563320; RRID: AB_2738136 TCR a/b viogreen (REA652) Miltenyi Cat# 130-119-709; RRID: AB_2751815 TCRa/b FITC (IP26) Biolegend Cat# 306706; RRID: AB_314644 TCRgd purified (11F2) Miltenyi Biotec Produced at request TCRy/d FITC (B1) Biolegend Cat# 331208; RRID: AB_1575108 TIGIT 153Eu (MBSA43) Fluidigm Cat# 3153019B; RRID:AB_2756419 TIGIT Dazzle (A15153G) Biolegend Cat# 372716; RRID: AB_2632931 Tim-3 Fitc (F38-2E2) eBioscience Cat# 11-3109-42; RRID: AB_2572488 TNFa Biolegend Cat# 502805; RRID: AB_2814397 TNF-a BV785 (FN50) BD Cat# 502948; RRID: AB_2565858 Chemicals, peptides, and recombinant proteins BD Horizon Brilliant Stain Buffer Becton Dickinson Cat# 563794 RBC lysis buffer 10X Biolegend Cat# 420301 Pierce 16% Formaldehyde (w/v), Methanol-free Thermo Fisher Cat# 28908 Fetal Bovine Serum PAN Biotec Cat# 3302 Stain Buffer (FBS) Becton Dickinson Cat# 554656 Pancoll human, Density: 1.077 g/ml Pan Biotech Cat# P04-601000 FcR Blocking Reagent, human Miltenyi Cat# 130-059-901 Cell-ID Intercalator-Ir Fluidigm Cat# 201192A Permeabilization buffer 10X eBioscience Cat# 00-8333-56 Maxpar PBS Fluidigm Cat# 201058 Maxpar Cell Staining buffer Fluidigm Cat# 201068 Maxpar X8 Multimetal Labeling Kit Fluidigm Cat# 201300 Proteomic stabilizer Smart Tube Inc. Cat# PROT1 KAPA HiFi HotStart Ready Mix Roche Cat# KK2601 Human Tru Stain FcX Biolegend Cat# 422301 SPRIselect Beckmann Coulter Cat# B23318 MagniSort Negative Selection Beads Thermo Fisher Cat# MSNB-6002-74 Lysercell WDF Sysmex Cat# AL-337-564 Fluorocell WDF Sysmex Cat# CV-377-552 IL2(IS) Milenyi Cat# 130-097-748 IFNa Milenyi Cat# 130-095-066 IL10 Immunotools Cat# 11340103 IL6 Immunotools Cat# 11340064 Amphiregulin PeproTech Cat# 100-55B TNFa Immunotools Cat# 11343015 Human IFN-g1b premium grade Miltenyi Biotec Cat# 130-096-481 Antibiotic-Antimyotic GIBCO Life Cat# 15240-062 Human Serum AB Plasma Sigma Cat# H3667-100ml Fetal bovine serum low in endotoxin A.H. Sigma Aldrich Cat# F7524-500ml (Continued on next page) Immunity 54, 2650–2669.e1–e14, November 9, 2021 e4

    Recombinant:

    Article Title: Early IFN-α signatures and persistent dysfunction are distinguishing features of NK cells in severe COVID-19.
    Article Snippet: .. REAGENT or RESOURCES SOURCE IDENTIFIER RANK purified (80704) R&D Systems Cat# MAB683; RRID:AB_2205330 RANKL APC Miltenyi Biotec Cat# 130-098-511; RRID:AB_2656691 SARS Cov-2 Spike specific nanobody AF488 https://doi.org/10.1126/ science.abe6230 N/A SARS-Cov-2 Nucleocapsid Sinobiological SIN-40588-V08B-100 Siglec 8 164Dy (7C9) Fluidigm Cat# 3164017B Siglec8 PE/Cy7 (7C9) Biolegend Cat# 347112; RRID:AB_2629720 Streptavidin BV786 Biolegend Cat# 405249 TBET BV711 (16893) BD Cat# 563320; RRID: AB_2738136 TCR a/b viogreen (REA652) Miltenyi Cat# 130-119-709; RRID: AB_2751815 TCRa/b FITC (IP26) Biolegend Cat# 306706; RRID: AB_314644 TCRgd purified (11F2) Miltenyi Biotec Produced at request TCRy/d FITC (B1) Biolegend Cat# 331208; RRID: AB_1575108 TIGIT 153Eu (MBSA43) Fluidigm Cat# 3153019B; RRID:AB_2756419 TIGIT Dazzle (A15153G) Biolegend Cat# 372716; RRID: AB_2632931 Tim-3 Fitc (F38-2E2) eBioscience Cat# 11-3109-42; RRID: AB_2572488 TNFa Biolegend Cat# 502805; RRID: AB_2814397 TNF-a BV785 (FN50) BD Cat# 502948; RRID: AB_2565858 Chemicals, peptides, and recombinant proteins BD Horizon Brilliant Stain Buffer Becton Dickinson Cat# 563794 RBC lysis buffer 10X Biolegend Cat# 420301 Pierce 16% Formaldehyde (w/v), Methanol-free Thermo Fisher Cat# 28908 Fetal Bovine Serum PAN Biotec Cat# 3302 Stain Buffer (FBS) Becton Dickinson Cat# 554656 Pancoll human, Density: 1.077 g/ml Pan Biotech Cat# P04-601000 FcR Blocking Reagent, human Miltenyi Cat# 130-059-901 Cell-ID Intercalator-Ir Fluidigm Cat# 201192A Permeabilization buffer 10X eBioscience Cat# 00-8333-56 Maxpar PBS Fluidigm Cat# 201058 Maxpar Cell Staining buffer Fluidigm Cat# 201068 Maxpar X8 Multimetal Labeling Kit Fluidigm Cat# 201300 Proteomic stabilizer Smart Tube Inc. Cat# PROT1 KAPA HiFi HotStart Ready Mix Roche Cat# KK2601 Human Tru Stain FcX Biolegend Cat# 422301 SPRIselect Beckmann Coulter Cat# B23318 MagniSort Negative Selection Beads Thermo Fisher Cat# MSNB-6002-74 Lysercell WDF Sysmex Cat# AL-337-564 Fluorocell WDF Sysmex Cat# CV-377-552 IL2(IS) Milenyi Cat# 130-097-748 IFNa Milenyi Cat# 130-095-066 IL10 Immunotools Cat# 11340103 IL6 Immunotools Cat# 11340064 Amphiregulin PeproTech Cat# 100-55B TNFa Immunotools Cat# 11343015 Human IFN-g1b premium grade Miltenyi Biotec Cat# 130-096-481 Antibiotic-Antimyotic GIBCO Life Cat# 15240-062 Human Serum AB Plasma Sigma Cat# H3667-100ml Fetal bovine serum low in endotoxin A.H. Sigma Aldrich Cat# F7524-500ml (Continued on next page) Immunity 54, 2650–2669.e1–e14, November 9, 2021 e4 .. REAGENT or RESOURCES SOURCE IDENTIFIER RANK purified (80704) R&D Systems Cat# MAB683; RRID:AB_2205330 RANKL APC Miltenyi Biotec Cat# 130-098-511; RRID:AB_2656691 SARS Cov-2 Spike specific nanobody AF488 https://doi.org/10.1126/ science.abe6230 N/A SARS-Cov-2 Nucleocapsid Sinobiological SIN-40588-V08B-100 Siglec 8 164Dy (7C9) Fluidigm Cat# 3164017B Siglec8 PE/Cy7 (7C9) Biolegend Cat# 347112; RRID:AB_2629720 Streptavidin BV786 Biolegend Cat# 405249 TBET BV711 (16893) BD Cat# 563320; RRID: AB_2738136 TCR a/b viogreen (REA652) Miltenyi Cat# 130-119-709; RRID: AB_2751815 TCRa/b FITC (IP26) Biolegend Cat# 306706; RRID: AB_314644 TCRgd purified (11F2) Miltenyi Biotec Produced at request TCRy/d FITC (B1) Biolegend Cat# 331208; RRID: AB_1575108 TIGIT 153Eu (MBSA43) Fluidigm Cat# 3153019B; RRID:AB_2756419 TIGIT Dazzle (A15153G) Biolegend Cat# 372716; RRID: AB_2632931 Tim-3 Fitc (F38-2E2) eBioscience Cat# 11-3109-42; RRID: AB_2572488 TNFa Biolegend Cat# 502805; RRID: AB_2814397 TNF-a BV785 (FN50) BD Cat# 502948; RRID: AB_2565858 Chemicals, peptides, and recombinant proteins BD Horizon Brilliant Stain Buffer Becton Dickinson Cat# 563794 RBC lysis buffer 10X Biolegend Cat# 420301 Pierce 16% Formaldehyde (w/v), Methanol-free Thermo Fisher Cat# 28908 Fetal Bovine Serum PAN Biotec Cat# 3302 Stain Buffer (FBS) Becton Dickinson Cat# 554656 Pancoll human, Density: 1.077 g/ml Pan Biotech Cat# P04-601000 FcR Blocking Reagent, human Miltenyi Cat# 130-059-901 Cell-ID Intercalator-Ir Fluidigm Cat# 201192A Permeabilization buffer 10X eBioscience Cat# 00-8333-56 Maxpar PBS Fluidigm Cat# 201058 Maxpar Cell Staining buffer Fluidigm Cat# 201068 Maxpar X8 Multimetal Labeling Kit Fluidigm Cat# 201300 Proteomic stabilizer Smart Tube Inc. Cat# PROT1 KAPA HiFi HotStart Ready Mix Roche Cat# KK2601 Human Tru Stain FcX Biolegend Cat# 422301 SPRIselect Beckmann Coulter Cat# B23318 MagniSort Negative Selection Beads Thermo Fisher Cat# MSNB-6002-74 Lysercell WDF Sysmex Cat# AL-337-564 Fluorocell WDF Sysmex Cat# CV-377-552 IL2(IS) Milenyi Cat# 130-097-748 IFNa Milenyi Cat# 130-095-066 IL10 Immunotools Cat# 11340103 IL6 Immunotools Cat# 11340064 Amphiregulin PeproTech Cat# 100-55B TNFa Immunotools Cat# 11343015 Human IFN-g1b premium grade Miltenyi Biotec Cat# 130-096-481 Antibiotic-Antimyotic GIBCO Life Cat# 15240-062 Human Serum AB Plasma Sigma Cat# H3667-100ml Fetal bovine serum low in endotoxin A.H. Sigma Aldrich Cat# F7524-500ml (Continued on next page) Immunity 54, 2650–2669.e1–e14, November 9, 2021 e4

    Staining:

    Article Title: Early IFN-α signatures and persistent dysfunction are distinguishing features of NK cells in severe COVID-19.
    Article Snippet: .. REAGENT or RESOURCES SOURCE IDENTIFIER RANK purified (80704) R&D Systems Cat# MAB683; RRID:AB_2205330 RANKL APC Miltenyi Biotec Cat# 130-098-511; RRID:AB_2656691 SARS Cov-2 Spike specific nanobody AF488 https://doi.org/10.1126/ science.abe6230 N/A SARS-Cov-2 Nucleocapsid Sinobiological SIN-40588-V08B-100 Siglec 8 164Dy (7C9) Fluidigm Cat# 3164017B Siglec8 PE/Cy7 (7C9) Biolegend Cat# 347112; RRID:AB_2629720 Streptavidin BV786 Biolegend Cat# 405249 TBET BV711 (16893) BD Cat# 563320; RRID: AB_2738136 TCR a/b viogreen (REA652) Miltenyi Cat# 130-119-709; RRID: AB_2751815 TCRa/b FITC (IP26) Biolegend Cat# 306706; RRID: AB_314644 TCRgd purified (11F2) Miltenyi Biotec Produced at request TCRy/d FITC (B1) Biolegend Cat# 331208; RRID: AB_1575108 TIGIT 153Eu (MBSA43) Fluidigm Cat# 3153019B; RRID:AB_2756419 TIGIT Dazzle (A15153G) Biolegend Cat# 372716; RRID: AB_2632931 Tim-3 Fitc (F38-2E2) eBioscience Cat# 11-3109-42; RRID: AB_2572488 TNFa Biolegend Cat# 502805; RRID: AB_2814397 TNF-a BV785 (FN50) BD Cat# 502948; RRID: AB_2565858 Chemicals, peptides, and recombinant proteins BD Horizon Brilliant Stain Buffer Becton Dickinson Cat# 563794 RBC lysis buffer 10X Biolegend Cat# 420301 Pierce 16% Formaldehyde (w/v), Methanol-free Thermo Fisher Cat# 28908 Fetal Bovine Serum PAN Biotec Cat# 3302 Stain Buffer (FBS) Becton Dickinson Cat# 554656 Pancoll human, Density: 1.077 g/ml Pan Biotech Cat# P04-601000 FcR Blocking Reagent, human Miltenyi Cat# 130-059-901 Cell-ID Intercalator-Ir Fluidigm Cat# 201192A Permeabilization buffer 10X eBioscience Cat# 00-8333-56 Maxpar PBS Fluidigm Cat# 201058 Maxpar Cell Staining buffer Fluidigm Cat# 201068 Maxpar X8 Multimetal Labeling Kit Fluidigm Cat# 201300 Proteomic stabilizer Smart Tube Inc. Cat# PROT1 KAPA HiFi HotStart Ready Mix Roche Cat# KK2601 Human Tru Stain FcX Biolegend Cat# 422301 SPRIselect Beckmann Coulter Cat# B23318 MagniSort Negative Selection Beads Thermo Fisher Cat# MSNB-6002-74 Lysercell WDF Sysmex Cat# AL-337-564 Fluorocell WDF Sysmex Cat# CV-377-552 IL2(IS) Milenyi Cat# 130-097-748 IFNa Milenyi Cat# 130-095-066 IL10 Immunotools Cat# 11340103 IL6 Immunotools Cat# 11340064 Amphiregulin PeproTech Cat# 100-55B TNFa Immunotools Cat# 11343015 Human IFN-g1b premium grade Miltenyi Biotec Cat# 130-096-481 Antibiotic-Antimyotic GIBCO Life Cat# 15240-062 Human Serum AB Plasma Sigma Cat# H3667-100ml Fetal bovine serum low in endotoxin A.H. Sigma Aldrich Cat# F7524-500ml (Continued on next page) Immunity 54, 2650–2669.e1–e14, November 9, 2021 e4 .. REAGENT or RESOURCES SOURCE IDENTIFIER RANK purified (80704) R&D Systems Cat# MAB683; RRID:AB_2205330 RANKL APC Miltenyi Biotec Cat# 130-098-511; RRID:AB_2656691 SARS Cov-2 Spike specific nanobody AF488 https://doi.org/10.1126/ science.abe6230 N/A SARS-Cov-2 Nucleocapsid Sinobiological SIN-40588-V08B-100 Siglec 8 164Dy (7C9) Fluidigm Cat# 3164017B Siglec8 PE/Cy7 (7C9) Biolegend Cat# 347112; RRID:AB_2629720 Streptavidin BV786 Biolegend Cat# 405249 TBET BV711 (16893) BD Cat# 563320; RRID: AB_2738136 TCR a/b viogreen (REA652) Miltenyi Cat# 130-119-709; RRID: AB_2751815 TCRa/b FITC (IP26) Biolegend Cat# 306706; RRID: AB_314644 TCRgd purified (11F2) Miltenyi Biotec Produced at request TCRy/d FITC (B1) Biolegend Cat# 331208; RRID: AB_1575108 TIGIT 153Eu (MBSA43) Fluidigm Cat# 3153019B; RRID:AB_2756419 TIGIT Dazzle (A15153G) Biolegend Cat# 372716; RRID: AB_2632931 Tim-3 Fitc (F38-2E2) eBioscience Cat# 11-3109-42; RRID: AB_2572488 TNFa Biolegend Cat# 502805; RRID: AB_2814397 TNF-a BV785 (FN50) BD Cat# 502948; RRID: AB_2565858 Chemicals, peptides, and recombinant proteins BD Horizon Brilliant Stain Buffer Becton Dickinson Cat# 563794 RBC lysis buffer 10X Biolegend Cat# 420301 Pierce 16% Formaldehyde (w/v), Methanol-free Thermo Fisher Cat# 28908 Fetal Bovine Serum PAN Biotec Cat# 3302 Stain Buffer (FBS) Becton Dickinson Cat# 554656 Pancoll human, Density: 1.077 g/ml Pan Biotech Cat# P04-601000 FcR Blocking Reagent, human Miltenyi Cat# 130-059-901 Cell-ID Intercalator-Ir Fluidigm Cat# 201192A Permeabilization buffer 10X eBioscience Cat# 00-8333-56 Maxpar PBS Fluidigm Cat# 201058 Maxpar Cell Staining buffer Fluidigm Cat# 201068 Maxpar X8 Multimetal Labeling Kit Fluidigm Cat# 201300 Proteomic stabilizer Smart Tube Inc. Cat# PROT1 KAPA HiFi HotStart Ready Mix Roche Cat# KK2601 Human Tru Stain FcX Biolegend Cat# 422301 SPRIselect Beckmann Coulter Cat# B23318 MagniSort Negative Selection Beads Thermo Fisher Cat# MSNB-6002-74 Lysercell WDF Sysmex Cat# AL-337-564 Fluorocell WDF Sysmex Cat# CV-377-552 IL2(IS) Milenyi Cat# 130-097-748 IFNa Milenyi Cat# 130-095-066 IL10 Immunotools Cat# 11340103 IL6 Immunotools Cat# 11340064 Amphiregulin PeproTech Cat# 100-55B TNFa Immunotools Cat# 11343015 Human IFN-g1b premium grade Miltenyi Biotec Cat# 130-096-481 Antibiotic-Antimyotic GIBCO Life Cat# 15240-062 Human Serum AB Plasma Sigma Cat# H3667-100ml Fetal bovine serum low in endotoxin A.H. Sigma Aldrich Cat# F7524-500ml (Continued on next page) Immunity 54, 2650–2669.e1–e14, November 9, 2021 e4

    Article Title: Tissue adaptation and clonal segregation of human memory T cells in barrier sites.
    Article Snippet: .. For antibodies with catalog numbers 3089003B, 3153019B, 3173001B, 3209003B: Per Fluidigm, "Each lot of conjugated antibody is quality control tested by CyTOF® analysis of stained cells using the appropriate positive and negative cell staining and/or activation controls." .. For antibodies with catalog number 555513: Per BD Biosciences, "All flow cytometry reagents are titrated on the relevant positive or negative cells.

    Red Blood Cell Lysis:

    Article Title: Early IFN-α signatures and persistent dysfunction are distinguishing features of NK cells in severe COVID-19.
    Article Snippet: .. REAGENT or RESOURCES SOURCE IDENTIFIER RANK purified (80704) R&D Systems Cat# MAB683; RRID:AB_2205330 RANKL APC Miltenyi Biotec Cat# 130-098-511; RRID:AB_2656691 SARS Cov-2 Spike specific nanobody AF488 https://doi.org/10.1126/ science.abe6230 N/A SARS-Cov-2 Nucleocapsid Sinobiological SIN-40588-V08B-100 Siglec 8 164Dy (7C9) Fluidigm Cat# 3164017B Siglec8 PE/Cy7 (7C9) Biolegend Cat# 347112; RRID:AB_2629720 Streptavidin BV786 Biolegend Cat# 405249 TBET BV711 (16893) BD Cat# 563320; RRID: AB_2738136 TCR a/b viogreen (REA652) Miltenyi Cat# 130-119-709; RRID: AB_2751815 TCRa/b FITC (IP26) Biolegend Cat# 306706; RRID: AB_314644 TCRgd purified (11F2) Miltenyi Biotec Produced at request TCRy/d FITC (B1) Biolegend Cat# 331208; RRID: AB_1575108 TIGIT 153Eu (MBSA43) Fluidigm Cat# 3153019B; RRID:AB_2756419 TIGIT Dazzle (A15153G) Biolegend Cat# 372716; RRID: AB_2632931 Tim-3 Fitc (F38-2E2) eBioscience Cat# 11-3109-42; RRID: AB_2572488 TNFa Biolegend Cat# 502805; RRID: AB_2814397 TNF-a BV785 (FN50) BD Cat# 502948; RRID: AB_2565858 Chemicals, peptides, and recombinant proteins BD Horizon Brilliant Stain Buffer Becton Dickinson Cat# 563794 RBC lysis buffer 10X Biolegend Cat# 420301 Pierce 16% Formaldehyde (w/v), Methanol-free Thermo Fisher Cat# 28908 Fetal Bovine Serum PAN Biotec Cat# 3302 Stain Buffer (FBS) Becton Dickinson Cat# 554656 Pancoll human, Density: 1.077 g/ml Pan Biotech Cat# P04-601000 FcR Blocking Reagent, human Miltenyi Cat# 130-059-901 Cell-ID Intercalator-Ir Fluidigm Cat# 201192A Permeabilization buffer 10X eBioscience Cat# 00-8333-56 Maxpar PBS Fluidigm Cat# 201058 Maxpar Cell Staining buffer Fluidigm Cat# 201068 Maxpar X8 Multimetal Labeling Kit Fluidigm Cat# 201300 Proteomic stabilizer Smart Tube Inc. Cat# PROT1 KAPA HiFi HotStart Ready Mix Roche Cat# KK2601 Human Tru Stain FcX Biolegend Cat# 422301 SPRIselect Beckmann Coulter Cat# B23318 MagniSort Negative Selection Beads Thermo Fisher Cat# MSNB-6002-74 Lysercell WDF Sysmex Cat# AL-337-564 Fluorocell WDF Sysmex Cat# CV-377-552 IL2(IS) Milenyi Cat# 130-097-748 IFNa Milenyi Cat# 130-095-066 IL10 Immunotools Cat# 11340103 IL6 Immunotools Cat# 11340064 Amphiregulin PeproTech Cat# 100-55B TNFa Immunotools Cat# 11343015 Human IFN-g1b premium grade Miltenyi Biotec Cat# 130-096-481 Antibiotic-Antimyotic GIBCO Life Cat# 15240-062 Human Serum AB Plasma Sigma Cat# H3667-100ml Fetal bovine serum low in endotoxin A.H. Sigma Aldrich Cat# F7524-500ml (Continued on next page) Immunity 54, 2650–2669.e1–e14, November 9, 2021 e4 .. REAGENT or RESOURCES SOURCE IDENTIFIER RANK purified (80704) R&D Systems Cat# MAB683; RRID:AB_2205330 RANKL APC Miltenyi Biotec Cat# 130-098-511; RRID:AB_2656691 SARS Cov-2 Spike specific nanobody AF488 https://doi.org/10.1126/ science.abe6230 N/A SARS-Cov-2 Nucleocapsid Sinobiological SIN-40588-V08B-100 Siglec 8 164Dy (7C9) Fluidigm Cat# 3164017B Siglec8 PE/Cy7 (7C9) Biolegend Cat# 347112; RRID:AB_2629720 Streptavidin BV786 Biolegend Cat# 405249 TBET BV711 (16893) BD Cat# 563320; RRID: AB_2738136 TCR a/b viogreen (REA652) Miltenyi Cat# 130-119-709; RRID: AB_2751815 TCRa/b FITC (IP26) Biolegend Cat# 306706; RRID: AB_314644 TCRgd purified (11F2) Miltenyi Biotec Produced at request TCRy/d FITC (B1) Biolegend Cat# 331208; RRID: AB_1575108 TIGIT 153Eu (MBSA43) Fluidigm Cat# 3153019B; RRID:AB_2756419 TIGIT Dazzle (A15153G) Biolegend Cat# 372716; RRID: AB_2632931 Tim-3 Fitc (F38-2E2) eBioscience Cat# 11-3109-42; RRID: AB_2572488 TNFa Biolegend Cat# 502805; RRID: AB_2814397 TNF-a BV785 (FN50) BD Cat# 502948; RRID: AB_2565858 Chemicals, peptides, and recombinant proteins BD Horizon Brilliant Stain Buffer Becton Dickinson Cat# 563794 RBC lysis buffer 10X Biolegend Cat# 420301 Pierce 16% Formaldehyde (w/v), Methanol-free Thermo Fisher Cat# 28908 Fetal Bovine Serum PAN Biotec Cat# 3302 Stain Buffer (FBS) Becton Dickinson Cat# 554656 Pancoll human, Density: 1.077 g/ml Pan Biotech Cat# P04-601000 FcR Blocking Reagent, human Miltenyi Cat# 130-059-901 Cell-ID Intercalator-Ir Fluidigm Cat# 201192A Permeabilization buffer 10X eBioscience Cat# 00-8333-56 Maxpar PBS Fluidigm Cat# 201058 Maxpar Cell Staining buffer Fluidigm Cat# 201068 Maxpar X8 Multimetal Labeling Kit Fluidigm Cat# 201300 Proteomic stabilizer Smart Tube Inc. Cat# PROT1 KAPA HiFi HotStart Ready Mix Roche Cat# KK2601 Human Tru Stain FcX Biolegend Cat# 422301 SPRIselect Beckmann Coulter Cat# B23318 MagniSort Negative Selection Beads Thermo Fisher Cat# MSNB-6002-74 Lysercell WDF Sysmex Cat# AL-337-564 Fluorocell WDF Sysmex Cat# CV-377-552 IL2(IS) Milenyi Cat# 130-097-748 IFNa Milenyi Cat# 130-095-066 IL10 Immunotools Cat# 11340103 IL6 Immunotools Cat# 11340064 Amphiregulin PeproTech Cat# 100-55B TNFa Immunotools Cat# 11343015 Human IFN-g1b premium grade Miltenyi Biotec Cat# 130-096-481 Antibiotic-Antimyotic GIBCO Life Cat# 15240-062 Human Serum AB Plasma Sigma Cat# H3667-100ml Fetal bovine serum low in endotoxin A.H. Sigma Aldrich Cat# F7524-500ml (Continued on next page) Immunity 54, 2650–2669.e1–e14, November 9, 2021 e4

    Blocking Assay:

    Article Title: Early IFN-α signatures and persistent dysfunction are distinguishing features of NK cells in severe COVID-19.
    Article Snippet: .. REAGENT or RESOURCES SOURCE IDENTIFIER RANK purified (80704) R&D Systems Cat# MAB683; RRID:AB_2205330 RANKL APC Miltenyi Biotec Cat# 130-098-511; RRID:AB_2656691 SARS Cov-2 Spike specific nanobody AF488 https://doi.org/10.1126/ science.abe6230 N/A SARS-Cov-2 Nucleocapsid Sinobiological SIN-40588-V08B-100 Siglec 8 164Dy (7C9) Fluidigm Cat# 3164017B Siglec8 PE/Cy7 (7C9) Biolegend Cat# 347112; RRID:AB_2629720 Streptavidin BV786 Biolegend Cat# 405249 TBET BV711 (16893) BD Cat# 563320; RRID: AB_2738136 TCR a/b viogreen (REA652) Miltenyi Cat# 130-119-709; RRID: AB_2751815 TCRa/b FITC (IP26) Biolegend Cat# 306706; RRID: AB_314644 TCRgd purified (11F2) Miltenyi Biotec Produced at request TCRy/d FITC (B1) Biolegend Cat# 331208; RRID: AB_1575108 TIGIT 153Eu (MBSA43) Fluidigm Cat# 3153019B; RRID:AB_2756419 TIGIT Dazzle (A15153G) Biolegend Cat# 372716; RRID: AB_2632931 Tim-3 Fitc (F38-2E2) eBioscience Cat# 11-3109-42; RRID: AB_2572488 TNFa Biolegend Cat# 502805; RRID: AB_2814397 TNF-a BV785 (FN50) BD Cat# 502948; RRID: AB_2565858 Chemicals, peptides, and recombinant proteins BD Horizon Brilliant Stain Buffer Becton Dickinson Cat# 563794 RBC lysis buffer 10X Biolegend Cat# 420301 Pierce 16% Formaldehyde (w/v), Methanol-free Thermo Fisher Cat# 28908 Fetal Bovine Serum PAN Biotec Cat# 3302 Stain Buffer (FBS) Becton Dickinson Cat# 554656 Pancoll human, Density: 1.077 g/ml Pan Biotech Cat# P04-601000 FcR Blocking Reagent, human Miltenyi Cat# 130-059-901 Cell-ID Intercalator-Ir Fluidigm Cat# 201192A Permeabilization buffer 10X eBioscience Cat# 00-8333-56 Maxpar PBS Fluidigm Cat# 201058 Maxpar Cell Staining buffer Fluidigm Cat# 201068 Maxpar X8 Multimetal Labeling Kit Fluidigm Cat# 201300 Proteomic stabilizer Smart Tube Inc. Cat# PROT1 KAPA HiFi HotStart Ready Mix Roche Cat# KK2601 Human Tru Stain FcX Biolegend Cat# 422301 SPRIselect Beckmann Coulter Cat# B23318 MagniSort Negative Selection Beads Thermo Fisher Cat# MSNB-6002-74 Lysercell WDF Sysmex Cat# AL-337-564 Fluorocell WDF Sysmex Cat# CV-377-552 IL2(IS) Milenyi Cat# 130-097-748 IFNa Milenyi Cat# 130-095-066 IL10 Immunotools Cat# 11340103 IL6 Immunotools Cat# 11340064 Amphiregulin PeproTech Cat# 100-55B TNFa Immunotools Cat# 11343015 Human IFN-g1b premium grade Miltenyi Biotec Cat# 130-096-481 Antibiotic-Antimyotic GIBCO Life Cat# 15240-062 Human Serum AB Plasma Sigma Cat# H3667-100ml Fetal bovine serum low in endotoxin A.H. Sigma Aldrich Cat# F7524-500ml (Continued on next page) Immunity 54, 2650–2669.e1–e14, November 9, 2021 e4 .. REAGENT or RESOURCES SOURCE IDENTIFIER RANK purified (80704) R&D Systems Cat# MAB683; RRID:AB_2205330 RANKL APC Miltenyi Biotec Cat# 130-098-511; RRID:AB_2656691 SARS Cov-2 Spike specific nanobody AF488 https://doi.org/10.1126/ science.abe6230 N/A SARS-Cov-2 Nucleocapsid Sinobiological SIN-40588-V08B-100 Siglec 8 164Dy (7C9) Fluidigm Cat# 3164017B Siglec8 PE/Cy7 (7C9) Biolegend Cat# 347112; RRID:AB_2629720 Streptavidin BV786 Biolegend Cat# 405249 TBET BV711 (16893) BD Cat# 563320; RRID: AB_2738136 TCR a/b viogreen (REA652) Miltenyi Cat# 130-119-709; RRID: AB_2751815 TCRa/b FITC (IP26) Biolegend Cat# 306706; RRID: AB_314644 TCRgd purified (11F2) Miltenyi Biotec Produced at request TCRy/d FITC (B1) Biolegend Cat# 331208; RRID: AB_1575108 TIGIT 153Eu (MBSA43) Fluidigm Cat# 3153019B; RRID:AB_2756419 TIGIT Dazzle (A15153G) Biolegend Cat# 372716; RRID: AB_2632931 Tim-3 Fitc (F38-2E2) eBioscience Cat# 11-3109-42; RRID: AB_2572488 TNFa Biolegend Cat# 502805; RRID: AB_2814397 TNF-a BV785 (FN50) BD Cat# 502948; RRID: AB_2565858 Chemicals, peptides, and recombinant proteins BD Horizon Brilliant Stain Buffer Becton Dickinson Cat# 563794 RBC lysis buffer 10X Biolegend Cat# 420301 Pierce 16% Formaldehyde (w/v), Methanol-free Thermo Fisher Cat# 28908 Fetal Bovine Serum PAN Biotec Cat# 3302 Stain Buffer (FBS) Becton Dickinson Cat# 554656 Pancoll human, Density: 1.077 g/ml Pan Biotech Cat# P04-601000 FcR Blocking Reagent, human Miltenyi Cat# 130-059-901 Cell-ID Intercalator-Ir Fluidigm Cat# 201192A Permeabilization buffer 10X eBioscience Cat# 00-8333-56 Maxpar PBS Fluidigm Cat# 201058 Maxpar Cell Staining buffer Fluidigm Cat# 201068 Maxpar X8 Multimetal Labeling Kit Fluidigm Cat# 201300 Proteomic stabilizer Smart Tube Inc. Cat# PROT1 KAPA HiFi HotStart Ready Mix Roche Cat# KK2601 Human Tru Stain FcX Biolegend Cat# 422301 SPRIselect Beckmann Coulter Cat# B23318 MagniSort Negative Selection Beads Thermo Fisher Cat# MSNB-6002-74 Lysercell WDF Sysmex Cat# AL-337-564 Fluorocell WDF Sysmex Cat# CV-377-552 IL2(IS) Milenyi Cat# 130-097-748 IFNa Milenyi Cat# 130-095-066 IL10 Immunotools Cat# 11340103 IL6 Immunotools Cat# 11340064 Amphiregulin PeproTech Cat# 100-55B TNFa Immunotools Cat# 11343015 Human IFN-g1b premium grade Miltenyi Biotec Cat# 130-096-481 Antibiotic-Antimyotic GIBCO Life Cat# 15240-062 Human Serum AB Plasma Sigma Cat# H3667-100ml Fetal bovine serum low in endotoxin A.H. Sigma Aldrich Cat# F7524-500ml (Continued on next page) Immunity 54, 2650–2669.e1–e14, November 9, 2021 e4

    Labeling:

    Article Title: Early IFN-α signatures and persistent dysfunction are distinguishing features of NK cells in severe COVID-19.
    Article Snippet: .. REAGENT or RESOURCES SOURCE IDENTIFIER RANK purified (80704) R&D Systems Cat# MAB683; RRID:AB_2205330 RANKL APC Miltenyi Biotec Cat# 130-098-511; RRID:AB_2656691 SARS Cov-2 Spike specific nanobody AF488 https://doi.org/10.1126/ science.abe6230 N/A SARS-Cov-2 Nucleocapsid Sinobiological SIN-40588-V08B-100 Siglec 8 164Dy (7C9) Fluidigm Cat# 3164017B Siglec8 PE/Cy7 (7C9) Biolegend Cat# 347112; RRID:AB_2629720 Streptavidin BV786 Biolegend Cat# 405249 TBET BV711 (16893) BD Cat# 563320; RRID: AB_2738136 TCR a/b viogreen (REA652) Miltenyi Cat# 130-119-709; RRID: AB_2751815 TCRa/b FITC (IP26) Biolegend Cat# 306706; RRID: AB_314644 TCRgd purified (11F2) Miltenyi Biotec Produced at request TCRy/d FITC (B1) Biolegend Cat# 331208; RRID: AB_1575108 TIGIT 153Eu (MBSA43) Fluidigm Cat# 3153019B; RRID:AB_2756419 TIGIT Dazzle (A15153G) Biolegend Cat# 372716; RRID: AB_2632931 Tim-3 Fitc (F38-2E2) eBioscience Cat# 11-3109-42; RRID: AB_2572488 TNFa Biolegend Cat# 502805; RRID: AB_2814397 TNF-a BV785 (FN50) BD Cat# 502948; RRID: AB_2565858 Chemicals, peptides, and recombinant proteins BD Horizon Brilliant Stain Buffer Becton Dickinson Cat# 563794 RBC lysis buffer 10X Biolegend Cat# 420301 Pierce 16% Formaldehyde (w/v), Methanol-free Thermo Fisher Cat# 28908 Fetal Bovine Serum PAN Biotec Cat# 3302 Stain Buffer (FBS) Becton Dickinson Cat# 554656 Pancoll human, Density: 1.077 g/ml Pan Biotech Cat# P04-601000 FcR Blocking Reagent, human Miltenyi Cat# 130-059-901 Cell-ID Intercalator-Ir Fluidigm Cat# 201192A Permeabilization buffer 10X eBioscience Cat# 00-8333-56 Maxpar PBS Fluidigm Cat# 201058 Maxpar Cell Staining buffer Fluidigm Cat# 201068 Maxpar X8 Multimetal Labeling Kit Fluidigm Cat# 201300 Proteomic stabilizer Smart Tube Inc. Cat# PROT1 KAPA HiFi HotStart Ready Mix Roche Cat# KK2601 Human Tru Stain FcX Biolegend Cat# 422301 SPRIselect Beckmann Coulter Cat# B23318 MagniSort Negative Selection Beads Thermo Fisher Cat# MSNB-6002-74 Lysercell WDF Sysmex Cat# AL-337-564 Fluorocell WDF Sysmex Cat# CV-377-552 IL2(IS) Milenyi Cat# 130-097-748 IFNa Milenyi Cat# 130-095-066 IL10 Immunotools Cat# 11340103 IL6 Immunotools Cat# 11340064 Amphiregulin PeproTech Cat# 100-55B TNFa Immunotools Cat# 11343015 Human IFN-g1b premium grade Miltenyi Biotec Cat# 130-096-481 Antibiotic-Antimyotic GIBCO Life Cat# 15240-062 Human Serum AB Plasma Sigma Cat# H3667-100ml Fetal bovine serum low in endotoxin A.H. Sigma Aldrich Cat# F7524-500ml (Continued on next page) Immunity 54, 2650–2669.e1–e14, November 9, 2021 e4 .. REAGENT or RESOURCES SOURCE IDENTIFIER RANK purified (80704) R&D Systems Cat# MAB683; RRID:AB_2205330 RANKL APC Miltenyi Biotec Cat# 130-098-511; RRID:AB_2656691 SARS Cov-2 Spike specific nanobody AF488 https://doi.org/10.1126/ science.abe6230 N/A SARS-Cov-2 Nucleocapsid Sinobiological SIN-40588-V08B-100 Siglec 8 164Dy (7C9) Fluidigm Cat# 3164017B Siglec8 PE/Cy7 (7C9) Biolegend Cat# 347112; RRID:AB_2629720 Streptavidin BV786 Biolegend Cat# 405249 TBET BV711 (16893) BD Cat# 563320; RRID: AB_2738136 TCR a/b viogreen (REA652) Miltenyi Cat# 130-119-709; RRID: AB_2751815 TCRa/b FITC (IP26) Biolegend Cat# 306706; RRID: AB_314644 TCRgd purified (11F2) Miltenyi Biotec Produced at request TCRy/d FITC (B1) Biolegend Cat# 331208; RRID: AB_1575108 TIGIT 153Eu (MBSA43) Fluidigm Cat# 3153019B; RRID:AB_2756419 TIGIT Dazzle (A15153G) Biolegend Cat# 372716; RRID: AB_2632931 Tim-3 Fitc (F38-2E2) eBioscience Cat# 11-3109-42; RRID: AB_2572488 TNFa Biolegend Cat# 502805; RRID: AB_2814397 TNF-a BV785 (FN50) BD Cat# 502948; RRID: AB_2565858 Chemicals, peptides, and recombinant proteins BD Horizon Brilliant Stain Buffer Becton Dickinson Cat# 563794 RBC lysis buffer 10X Biolegend Cat# 420301 Pierce 16% Formaldehyde (w/v), Methanol-free Thermo Fisher Cat# 28908 Fetal Bovine Serum PAN Biotec Cat# 3302 Stain Buffer (FBS) Becton Dickinson Cat# 554656 Pancoll human, Density: 1.077 g/ml Pan Biotech Cat# P04-601000 FcR Blocking Reagent, human Miltenyi Cat# 130-059-901 Cell-ID Intercalator-Ir Fluidigm Cat# 201192A Permeabilization buffer 10X eBioscience Cat# 00-8333-56 Maxpar PBS Fluidigm Cat# 201058 Maxpar Cell Staining buffer Fluidigm Cat# 201068 Maxpar X8 Multimetal Labeling Kit Fluidigm Cat# 201300 Proteomic stabilizer Smart Tube Inc. Cat# PROT1 KAPA HiFi HotStart Ready Mix Roche Cat# KK2601 Human Tru Stain FcX Biolegend Cat# 422301 SPRIselect Beckmann Coulter Cat# B23318 MagniSort Negative Selection Beads Thermo Fisher Cat# MSNB-6002-74 Lysercell WDF Sysmex Cat# AL-337-564 Fluorocell WDF Sysmex Cat# CV-377-552 IL2(IS) Milenyi Cat# 130-097-748 IFNa Milenyi Cat# 130-095-066 IL10 Immunotools Cat# 11340103 IL6 Immunotools Cat# 11340064 Amphiregulin PeproTech Cat# 100-55B TNFa Immunotools Cat# 11343015 Human IFN-g1b premium grade Miltenyi Biotec Cat# 130-096-481 Antibiotic-Antimyotic GIBCO Life Cat# 15240-062 Human Serum AB Plasma Sigma Cat# H3667-100ml Fetal bovine serum low in endotoxin A.H. Sigma Aldrich Cat# F7524-500ml (Continued on next page) Immunity 54, 2650–2669.e1–e14, November 9, 2021 e4

    Selection:

    Article Title: Early IFN-α signatures and persistent dysfunction are distinguishing features of NK cells in severe COVID-19.
    Article Snippet: .. REAGENT or RESOURCES SOURCE IDENTIFIER RANK purified (80704) R&D Systems Cat# MAB683; RRID:AB_2205330 RANKL APC Miltenyi Biotec Cat# 130-098-511; RRID:AB_2656691 SARS Cov-2 Spike specific nanobody AF488 https://doi.org/10.1126/ science.abe6230 N/A SARS-Cov-2 Nucleocapsid Sinobiological SIN-40588-V08B-100 Siglec 8 164Dy (7C9) Fluidigm Cat# 3164017B Siglec8 PE/Cy7 (7C9) Biolegend Cat# 347112; RRID:AB_2629720 Streptavidin BV786 Biolegend Cat# 405249 TBET BV711 (16893) BD Cat# 563320; RRID: AB_2738136 TCR a/b viogreen (REA652) Miltenyi Cat# 130-119-709; RRID: AB_2751815 TCRa/b FITC (IP26) Biolegend Cat# 306706; RRID: AB_314644 TCRgd purified (11F2) Miltenyi Biotec Produced at request TCRy/d FITC (B1) Biolegend Cat# 331208; RRID: AB_1575108 TIGIT 153Eu (MBSA43) Fluidigm Cat# 3153019B; RRID:AB_2756419 TIGIT Dazzle (A15153G) Biolegend Cat# 372716; RRID: AB_2632931 Tim-3 Fitc (F38-2E2) eBioscience Cat# 11-3109-42; RRID: AB_2572488 TNFa Biolegend Cat# 502805; RRID: AB_2814397 TNF-a BV785 (FN50) BD Cat# 502948; RRID: AB_2565858 Chemicals, peptides, and recombinant proteins BD Horizon Brilliant Stain Buffer Becton Dickinson Cat# 563794 RBC lysis buffer 10X Biolegend Cat# 420301 Pierce 16% Formaldehyde (w/v), Methanol-free Thermo Fisher Cat# 28908 Fetal Bovine Serum PAN Biotec Cat# 3302 Stain Buffer (FBS) Becton Dickinson Cat# 554656 Pancoll human, Density: 1.077 g/ml Pan Biotech Cat# P04-601000 FcR Blocking Reagent, human Miltenyi Cat# 130-059-901 Cell-ID Intercalator-Ir Fluidigm Cat# 201192A Permeabilization buffer 10X eBioscience Cat# 00-8333-56 Maxpar PBS Fluidigm Cat# 201058 Maxpar Cell Staining buffer Fluidigm Cat# 201068 Maxpar X8 Multimetal Labeling Kit Fluidigm Cat# 201300 Proteomic stabilizer Smart Tube Inc. Cat# PROT1 KAPA HiFi HotStart Ready Mix Roche Cat# KK2601 Human Tru Stain FcX Biolegend Cat# 422301 SPRIselect Beckmann Coulter Cat# B23318 MagniSort Negative Selection Beads Thermo Fisher Cat# MSNB-6002-74 Lysercell WDF Sysmex Cat# AL-337-564 Fluorocell WDF Sysmex Cat# CV-377-552 IL2(IS) Milenyi Cat# 130-097-748 IFNa Milenyi Cat# 130-095-066 IL10 Immunotools Cat# 11340103 IL6 Immunotools Cat# 11340064 Amphiregulin PeproTech Cat# 100-55B TNFa Immunotools Cat# 11343015 Human IFN-g1b premium grade Miltenyi Biotec Cat# 130-096-481 Antibiotic-Antimyotic GIBCO Life Cat# 15240-062 Human Serum AB Plasma Sigma Cat# H3667-100ml Fetal bovine serum low in endotoxin A.H. Sigma Aldrich Cat# F7524-500ml (Continued on next page) Immunity 54, 2650–2669.e1–e14, November 9, 2021 e4 .. REAGENT or RESOURCES SOURCE IDENTIFIER RANK purified (80704) R&D Systems Cat# MAB683; RRID:AB_2205330 RANKL APC Miltenyi Biotec Cat# 130-098-511; RRID:AB_2656691 SARS Cov-2 Spike specific nanobody AF488 https://doi.org/10.1126/ science.abe6230 N/A SARS-Cov-2 Nucleocapsid Sinobiological SIN-40588-V08B-100 Siglec 8 164Dy (7C9) Fluidigm Cat# 3164017B Siglec8 PE/Cy7 (7C9) Biolegend Cat# 347112; RRID:AB_2629720 Streptavidin BV786 Biolegend Cat# 405249 TBET BV711 (16893) BD Cat# 563320; RRID: AB_2738136 TCR a/b viogreen (REA652) Miltenyi Cat# 130-119-709; RRID: AB_2751815 TCRa/b FITC (IP26) Biolegend Cat# 306706; RRID: AB_314644 TCRgd purified (11F2) Miltenyi Biotec Produced at request TCRy/d FITC (B1) Biolegend Cat# 331208; RRID: AB_1575108 TIGIT 153Eu (MBSA43) Fluidigm Cat# 3153019B; RRID:AB_2756419 TIGIT Dazzle (A15153G) Biolegend Cat# 372716; RRID: AB_2632931 Tim-3 Fitc (F38-2E2) eBioscience Cat# 11-3109-42; RRID: AB_2572488 TNFa Biolegend Cat# 502805; RRID: AB_2814397 TNF-a BV785 (FN50) BD Cat# 502948; RRID: AB_2565858 Chemicals, peptides, and recombinant proteins BD Horizon Brilliant Stain Buffer Becton Dickinson Cat# 563794 RBC lysis buffer 10X Biolegend Cat# 420301 Pierce 16% Formaldehyde (w/v), Methanol-free Thermo Fisher Cat# 28908 Fetal Bovine Serum PAN Biotec Cat# 3302 Stain Buffer (FBS) Becton Dickinson Cat# 554656 Pancoll human, Density: 1.077 g/ml Pan Biotech Cat# P04-601000 FcR Blocking Reagent, human Miltenyi Cat# 130-059-901 Cell-ID Intercalator-Ir Fluidigm Cat# 201192A Permeabilization buffer 10X eBioscience Cat# 00-8333-56 Maxpar PBS Fluidigm Cat# 201058 Maxpar Cell Staining buffer Fluidigm Cat# 201068 Maxpar X8 Multimetal Labeling Kit Fluidigm Cat# 201300 Proteomic stabilizer Smart Tube Inc. Cat# PROT1 KAPA HiFi HotStart Ready Mix Roche Cat# KK2601 Human Tru Stain FcX Biolegend Cat# 422301 SPRIselect Beckmann Coulter Cat# B23318 MagniSort Negative Selection Beads Thermo Fisher Cat# MSNB-6002-74 Lysercell WDF Sysmex Cat# AL-337-564 Fluorocell WDF Sysmex Cat# CV-377-552 IL2(IS) Milenyi Cat# 130-097-748 IFNa Milenyi Cat# 130-095-066 IL10 Immunotools Cat# 11340103 IL6 Immunotools Cat# 11340064 Amphiregulin PeproTech Cat# 100-55B TNFa Immunotools Cat# 11343015 Human IFN-g1b premium grade Miltenyi Biotec Cat# 130-096-481 Antibiotic-Antimyotic GIBCO Life Cat# 15240-062 Human Serum AB Plasma Sigma Cat# H3667-100ml Fetal bovine serum low in endotoxin A.H. Sigma Aldrich Cat# F7524-500ml (Continued on next page) Immunity 54, 2650–2669.e1–e14, November 9, 2021 e4

    Clinical Proteomics:

    Article Title: Early IFN-α signatures and persistent dysfunction are distinguishing features of NK cells in severe COVID-19.
    Article Snippet: .. REAGENT or RESOURCES SOURCE IDENTIFIER RANK purified (80704) R&D Systems Cat# MAB683; RRID:AB_2205330 RANKL APC Miltenyi Biotec Cat# 130-098-511; RRID:AB_2656691 SARS Cov-2 Spike specific nanobody AF488 https://doi.org/10.1126/ science.abe6230 N/A SARS-Cov-2 Nucleocapsid Sinobiological SIN-40588-V08B-100 Siglec 8 164Dy (7C9) Fluidigm Cat# 3164017B Siglec8 PE/Cy7 (7C9) Biolegend Cat# 347112; RRID:AB_2629720 Streptavidin BV786 Biolegend Cat# 405249 TBET BV711 (16893) BD Cat# 563320; RRID: AB_2738136 TCR a/b viogreen (REA652) Miltenyi Cat# 130-119-709; RRID: AB_2751815 TCRa/b FITC (IP26) Biolegend Cat# 306706; RRID: AB_314644 TCRgd purified (11F2) Miltenyi Biotec Produced at request TCRy/d FITC (B1) Biolegend Cat# 331208; RRID: AB_1575108 TIGIT 153Eu (MBSA43) Fluidigm Cat# 3153019B; RRID:AB_2756419 TIGIT Dazzle (A15153G) Biolegend Cat# 372716; RRID: AB_2632931 Tim-3 Fitc (F38-2E2) eBioscience Cat# 11-3109-42; RRID: AB_2572488 TNFa Biolegend Cat# 502805; RRID: AB_2814397 TNF-a BV785 (FN50) BD Cat# 502948; RRID: AB_2565858 Chemicals, peptides, and recombinant proteins BD Horizon Brilliant Stain Buffer Becton Dickinson Cat# 563794 RBC lysis buffer 10X Biolegend Cat# 420301 Pierce 16% Formaldehyde (w/v), Methanol-free Thermo Fisher Cat# 28908 Fetal Bovine Serum PAN Biotec Cat# 3302 Stain Buffer (FBS) Becton Dickinson Cat# 554656 Pancoll human, Density: 1.077 g/ml Pan Biotech Cat# P04-601000 FcR Blocking Reagent, human Miltenyi Cat# 130-059-901 Cell-ID Intercalator-Ir Fluidigm Cat# 201192A Permeabilization buffer 10X eBioscience Cat# 00-8333-56 Maxpar PBS Fluidigm Cat# 201058 Maxpar Cell Staining buffer Fluidigm Cat# 201068 Maxpar X8 Multimetal Labeling Kit Fluidigm Cat# 201300 Proteomic stabilizer Smart Tube Inc. Cat# PROT1 KAPA HiFi HotStart Ready Mix Roche Cat# KK2601 Human Tru Stain FcX Biolegend Cat# 422301 SPRIselect Beckmann Coulter Cat# B23318 MagniSort Negative Selection Beads Thermo Fisher Cat# MSNB-6002-74 Lysercell WDF Sysmex Cat# AL-337-564 Fluorocell WDF Sysmex Cat# CV-377-552 IL2(IS) Milenyi Cat# 130-097-748 IFNa Milenyi Cat# 130-095-066 IL10 Immunotools Cat# 11340103 IL6 Immunotools Cat# 11340064 Amphiregulin PeproTech Cat# 100-55B TNFa Immunotools Cat# 11343015 Human IFN-g1b premium grade Miltenyi Biotec Cat# 130-096-481 Antibiotic-Antimyotic GIBCO Life Cat# 15240-062 Human Serum AB Plasma Sigma Cat# H3667-100ml Fetal bovine serum low in endotoxin A.H. Sigma Aldrich Cat# F7524-500ml (Continued on next page) Immunity 54, 2650–2669.e1–e14, November 9, 2021 e4 .. REAGENT or RESOURCES SOURCE IDENTIFIER RANK purified (80704) R&D Systems Cat# MAB683; RRID:AB_2205330 RANKL APC Miltenyi Biotec Cat# 130-098-511; RRID:AB_2656691 SARS Cov-2 Spike specific nanobody AF488 https://doi.org/10.1126/ science.abe6230 N/A SARS-Cov-2 Nucleocapsid Sinobiological SIN-40588-V08B-100 Siglec 8 164Dy (7C9) Fluidigm Cat# 3164017B Siglec8 PE/Cy7 (7C9) Biolegend Cat# 347112; RRID:AB_2629720 Streptavidin BV786 Biolegend Cat# 405249 TBET BV711 (16893) BD Cat# 563320; RRID: AB_2738136 TCR a/b viogreen (REA652) Miltenyi Cat# 130-119-709; RRID: AB_2751815 TCRa/b FITC (IP26) Biolegend Cat# 306706; RRID: AB_314644 TCRgd purified (11F2) Miltenyi Biotec Produced at request TCRy/d FITC (B1) Biolegend Cat# 331208; RRID: AB_1575108 TIGIT 153Eu (MBSA43) Fluidigm Cat# 3153019B; RRID:AB_2756419 TIGIT Dazzle (A15153G) Biolegend Cat# 372716; RRID: AB_2632931 Tim-3 Fitc (F38-2E2) eBioscience Cat# 11-3109-42; RRID: AB_2572488 TNFa Biolegend Cat# 502805; RRID: AB_2814397 TNF-a BV785 (FN50) BD Cat# 502948; RRID: AB_2565858 Chemicals, peptides, and recombinant proteins BD Horizon Brilliant Stain Buffer Becton Dickinson Cat# 563794 RBC lysis buffer 10X Biolegend Cat# 420301 Pierce 16% Formaldehyde (w/v), Methanol-free Thermo Fisher Cat# 28908 Fetal Bovine Serum PAN Biotec Cat# 3302 Stain Buffer (FBS) Becton Dickinson Cat# 554656 Pancoll human, Density: 1.077 g/ml Pan Biotech Cat# P04-601000 FcR Blocking Reagent, human Miltenyi Cat# 130-059-901 Cell-ID Intercalator-Ir Fluidigm Cat# 201192A Permeabilization buffer 10X eBioscience Cat# 00-8333-56 Maxpar PBS Fluidigm Cat# 201058 Maxpar Cell Staining buffer Fluidigm Cat# 201068 Maxpar X8 Multimetal Labeling Kit Fluidigm Cat# 201300 Proteomic stabilizer Smart Tube Inc. Cat# PROT1 KAPA HiFi HotStart Ready Mix Roche Cat# KK2601 Human Tru Stain FcX Biolegend Cat# 422301 SPRIselect Beckmann Coulter Cat# B23318 MagniSort Negative Selection Beads Thermo Fisher Cat# MSNB-6002-74 Lysercell WDF Sysmex Cat# AL-337-564 Fluorocell WDF Sysmex Cat# CV-377-552 IL2(IS) Milenyi Cat# 130-097-748 IFNa Milenyi Cat# 130-095-066 IL10 Immunotools Cat# 11340103 IL6 Immunotools Cat# 11340064 Amphiregulin PeproTech Cat# 100-55B TNFa Immunotools Cat# 11343015 Human IFN-g1b premium grade Miltenyi Biotec Cat# 130-096-481 Antibiotic-Antimyotic GIBCO Life Cat# 15240-062 Human Serum AB Plasma Sigma Cat# H3667-100ml Fetal bovine serum low in endotoxin A.H. Sigma Aldrich Cat# F7524-500ml (Continued on next page) Immunity 54, 2650–2669.e1–e14, November 9, 2021 e4

    other:

    Article Title: Delineation of the Human Germinal Centre Immune Landscape Using Multiplex Imaging Analysis
    Article Snippet: TIGIT , 153Eu , MBSA43 , Standard Biotools , 3153019B , 2 μL.

    Article Title: Neoadjuvant Fc-enhanced anti-CTLA-4 targets Tregs to augment androgen deprivation in high-risk prostate cancer: A randomized phase I trial
    Article Snippet: Anti-Human TIGIT (MBSA43) 153Eu , Standard BioTools , Cat# 3153019B.

    Article Title: Rewired type I IFN signaling is linked to age-dependent differences in COVID-19
    Article Snippet: 153Eu_TIGIT_MBSA43 , Standard BioTools , Cat#3153019B, RRID:AB_2756419.

    Article Title: Unsupervised Clustering of Cell Populations in Germinal Centers Using Multiplexed Immunofluorescence
    Article Snippet: TIGIT , 153Eu , MBSA43 , Standard Biotools , 3153019B , 2 μ L.

    Article Title: The life-saving benefit of dexamethasone in severe COVID-19 is linked to a reversal of monocyte dysregulation.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER GSVA (R package) Hänzelmann et al.59 v1.40.1 fgsea (R package) Korotkevich et al.60 v1.18.0 ggplot2 (R Package; bulk RNA-seq analysis) Wickham et al.61 v3.3.5 ggplot2 (R package; DNA methylation analysis) Wickham et al.61 v3.3.6 pheatmap (R package) Kolde et al.62 v1.0.12 sva (R package) Leek et al.63 v3.44.0 minfi (R package) Fortin et al.64 v1.42.0 limma (R package; DNA methylation analysis) Ritchie et al.65 v3.52.2 limma (R package; bulk RNA-seq analysis) Ritchie et al.65 v3.48.3 methylGSA (R package) Fortin et al.64 v1.14.0 FlowSOM (R package) Van Gassen et al.66 v3.17 uwot (R package) https://cran.r-project.org/web/packages/ uwot/index.html v0.1.8 ComplexHeatmap (R package) Gu et al.67 v1.20.0 FlowJo https://www.flowjo.com v10.8 JMP Pro SAS Institute inc. V 16.2.0 Matching (R package) Sekhon et al.68 v4.9-7 OMIQ www.omiq.ai N/A ll OPEN ACCESS Please cite this article in press as: Knoll et al., The life-saving benefit of dexamethasone in severe COVID-19 is linked to a reversal of monocyte dysregulation, Cell (2024), https://doi.org/10.1016/j.cell.2024.06.014 Article

    Control:

    Article Title: Tissue adaptation and clonal segregation of human memory T cells in barrier sites.
    Article Snippet: .. For antibodies with catalog numbers 3089003B, 3153019B, 3173001B, 3209003B: Per Fluidigm, "Each lot of conjugated antibody is quality control tested by CyTOF® analysis of stained cells using the appropriate positive and negative cell staining and/or activation controls." .. For antibodies with catalog number 555513: Per BD Biosciences, "All flow cytometry reagents are titrated on the relevant positive or negative cells.

    Activation Assay:

    Article Title: Tissue adaptation and clonal segregation of human memory T cells in barrier sites.
    Article Snippet: .. For antibodies with catalog numbers 3089003B, 3153019B, 3173001B, 3209003B: Per Fluidigm, "Each lot of conjugated antibody is quality control tested by CyTOF® analysis of stained cells using the appropriate positive and negative cell staining and/or activation controls." .. For antibodies with catalog number 555513: Per BD Biosciences, "All flow cytometry reagents are titrated on the relevant positive or negative cells.



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