Journal: STAR Protocols
Article Title: An optimized protocol for phenotyping human granulocytes by mass cytometry
doi: 10.1016/j.xpro.2022.101280
Figure Lengend Snippet: Optimization of eosinophil and neutrophil detection (A) Cells were treated with RBC lysis buffer, followed by staining with surface antibodies (including CD16 and CD66b) prior to fixation with PFA as outlined in protocol. (B) Cells were treated with RBC lysis buffer, fixed with PFA, and then stained with surface antibodies. (C) Cells were treated with a commercially available RBC lysis/fix solution prior to staining with surface antibodies.
Article Snippet: CD66b (Clone 80H3) - 152Sm , Fluidigm Sciences , Cat#3152011B.
Techniques: Red Blood Cell Lysis, Staining