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3151004b  (fluidigm)


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    fluidigm 3151004b
    3151004b, supplied by fluidigm, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/3151004b/Anti-Human+IgG+lambda+(MHL-38)-151Eu/pm40322080-147-65-69
    Average 92 stars, based on 2 article reviews
    3151004b - by Bioz Stars, 2026-09
    92/100 stars

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    Related Articles

    Clinical Proteomics:

    Article Title: Comprehensive analysis of B cell repopulation in ocrelizumab-treated patients with multiple sclerosis by mass cytometry and proteomics.
    Article Snippet: ArticleiScience Comprehensive analysis of B cell repopulation in ocrelizumab-treated patients with multiple sclerosis by mass cytometry and proteomics

    Recombinant:

    Article Title: Comprehensive analysis of B cell repopulation in ocrelizumab-treated patients with multiple sclerosis by mass cytometry and proteomics.
    Article Snippet: ArticleiScience Comprehensive analysis of B cell repopulation in ocrelizumab-treated patients with multiple sclerosis by mass cytometry and proteomics

    Staining:

    Article Title: Comprehensive analysis of B cell repopulation in ocrelizumab-treated patients with multiple sclerosis by mass cytometry and proteomics.
    Article Snippet: ArticleiScience Comprehensive analysis of B cell repopulation in ocrelizumab-treated patients with multiple sclerosis by mass cytometry and proteomics

    Software:

    Article Title: Comprehensive analysis of B cell repopulation in ocrelizumab-treated patients with multiple sclerosis by mass cytometry and proteomics.
    Article Snippet: ArticleiScience Comprehensive analysis of B cell repopulation in ocrelizumab-treated patients with multiple sclerosis by mass cytometry and proteomics



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    DDR in naïve lymphocyte subsets is differential to mature memory populations. PBMCs obtained from 26 healthy donors were irradiated with 2Gy and fixed at indicated time points. Surface markers of lymphocyte subsets and intranuclear DDR biomarkers were assessed by mass cytometry. Fold inductions of γH2AX, p-ATM, p-CHK2, and p53 were calculated in CD45 + lymphocyte subsets, based on mean fluorescence intensities normalized on unirradiated samples. T cell subsets were characterized as CD3 + , CD3 + CD4 + , CD3 + CD8 + , CD45RA + CCR7 + (naïve CD4/CD8), CD45RO + CCR7 +/- (central and effector memory CD4/CD8) (A) . NK lymphocyte subsets were defined as CD3 - CD56 bright CD16 - , CD3 - CD56 bright CD16 + , CD3 - CD56 dim CD16 + (B) , which were further stratified to CD57 expression on CD56 bright CD16 + and CD56 dim CD16 + subsets. CD3 - B lymphocytes were characterized as CD19 + CD20 + , CD27 - IgD + (naïve B), CD27 + (memory B), CD27 + IgM + (unswitched memory B) CD27 + IgM - IgGκ + (class switched memory B IgGκ), CD27 + IgM - IgGΛ + (class switched memory B IgGΛ), CD27 + IgM + IgD + (Marginal Zone (MZ)-like B), IgM ++ CD38 ++ (transitional B), CD27 + IgM + IgD - (IgM only B), CD27 - IgM - IgD - (atypical memory B), CD19 + CD20 - CD27 + CD38 + IgM + (unswitched plasmablasts), CD19 + CD20 - CD27 + CD38 + IgM - (class switched plasmablasts), and CD21 low CD38 low B cells. (C) Bars represent mean values of fold induction; error bars represent standard deviations. Significance is shown for naïve T and B cell subsets compared to memory subsets, and immature CD56 bright CD16 - to mature CD56 bright CD16 + and CD56 dim CD16 + NK lymphocytes (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001).

    Journal: Frontiers in Immunology

    Article Title: Differential DNA Damage Response of Peripheral Blood Lymphocyte Populations

    doi: 10.3389/fimmu.2021.739675

    Figure Lengend Snippet: DDR in naïve lymphocyte subsets is differential to mature memory populations. PBMCs obtained from 26 healthy donors were irradiated with 2Gy and fixed at indicated time points. Surface markers of lymphocyte subsets and intranuclear DDR biomarkers were assessed by mass cytometry. Fold inductions of γH2AX, p-ATM, p-CHK2, and p53 were calculated in CD45 + lymphocyte subsets, based on mean fluorescence intensities normalized on unirradiated samples. T cell subsets were characterized as CD3 + , CD3 + CD4 + , CD3 + CD8 + , CD45RA + CCR7 + (naïve CD4/CD8), CD45RO + CCR7 +/- (central and effector memory CD4/CD8) (A) . NK lymphocyte subsets were defined as CD3 - CD56 bright CD16 - , CD3 - CD56 bright CD16 + , CD3 - CD56 dim CD16 + (B) , which were further stratified to CD57 expression on CD56 bright CD16 + and CD56 dim CD16 + subsets. CD3 - B lymphocytes were characterized as CD19 + CD20 + , CD27 - IgD + (naïve B), CD27 + (memory B), CD27 + IgM + (unswitched memory B) CD27 + IgM - IgGκ + (class switched memory B IgGκ), CD27 + IgM - IgGΛ + (class switched memory B IgGΛ), CD27 + IgM + IgD + (Marginal Zone (MZ)-like B), IgM ++ CD38 ++ (transitional B), CD27 + IgM + IgD - (IgM only B), CD27 - IgM - IgD - (atypical memory B), CD19 + CD20 - CD27 + CD38 + IgM + (unswitched plasmablasts), CD19 + CD20 - CD27 + CD38 + IgM - (class switched plasmablasts), and CD21 low CD38 low B cells. (C) Bars represent mean values of fold induction; error bars represent standard deviations. Significance is shown for naïve T and B cell subsets compared to memory subsets, and immature CD56 bright CD16 - to mature CD56 bright CD16 + and CD56 dim CD16 + NK lymphocytes (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001).

    Article Snippet: The following surface antibodies were used at concentrations of 1μl/100μl staining buffer: Mouse anti-human CD45 (HI30)-154Sm (Fluidigm), anti-human CD3 (UCHT1)-170Er (Fluidigm), anti-human CD4 (SK3)-174Yb (Fluidigm), anti-Human CD8 (Sk1)-168Er (Fluidigm), anti-human CD45RA (HI100) 169Tm (Fluidigm), anti-human CD45RO (UCHL1) 164-Dy (Fluidigm), anti-human CD197/CCR7 (G053H7) 159Tb (Fluidigm), anti-human CD69 (FN50) 144Nd (Fluidigm), anti-human CD56 (HCD56) 176Yb (Fluidigm), anti-Human CD16 (3G8)-165Ho/Fluidigm, anti-human CD57 (HCD57) 163Dy (Fluidigm), anti-human CD19 (HIB19) 142Nd (Fluidigm), anti-human CD20 (2H7) 171Yb (Fluidigm), anti-human IgD (IA6-2) 146Nd (Fluidigm), anti-human IgM (MHM-88) 172Yb (Fluidigm), anti-human IgG kappa (MHK-49) 160Gd (Fluidigm), anti-human IgG lambda (MHL-38) 151Eu (Fluidigm), anti-human CD27 (L128) 158Gd (Fluidigm), anti-human CD38 (HIT2) 167Er (Fluidigm), anti-human CD21 (BL-13) 152Sm (Fluidigm).

    Techniques: Irradiation, Mass Cytometry, Fluorescence, Expressing

    Journal: eLife

    Article Title: Multiplexed imaging of immune cells in staged multiple sclerosis lesions by mass cytometry

    doi: 10.7554/eLife.48051

    Figure Lengend Snippet:

    Article Snippet: Antibody , Anti-human IgLambda-151-Eu (Mouse monoclonal) , Fluidigm , Cat#: 3151004B RRID: AB_2810853 , IMC: (1/3000).

    Techniques: