mouse anti human cd34 148nd  (fluidigm)

Journal: iScience

Article Title: Pharmacological inhibition of RAS overcomes FLT3 inhibitor resistance in FLT3-ITD+ AML through AP-1 and RUNX1

doi: 10.1016/j.isci.2024.109576

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Article Snippet: Mouse anti-human CD34-148Nd , Standard BioTools , Cat# 3148001B; RRID: AB_2810243.

Techniques: Control, Virus, Recombinant, Modification, Saline, Stripping Membranes, Protease Inhibitor, Reverse Transcription, Membrane, Labeling, Gel Extraction, Plasmid Preparation, RNA Library Preparation, Library Quantification, Software

Journal: Frontiers in Oncology

Article Title: Inter and intra-tumor heterogeneity of paediatric type diffuse high-grade gliomas revealed by single-cell mass cytometry

doi: 10.3389/fonc.2022.1016343

Figure Lengend Snippet: Summary of the 15 antibodies used for the mass cytometry analysis.

Article Snippet: Anti-Human CD34 , 149Sm , Fluidigm , 3149013B , AB_2756285.

Techniques: Mass Cytometry

Journal: Frontiers in Oncology

Article Title: Inter and intra-tumor heterogeneity of paediatric type diffuse high-grade gliomas revealed by single-cell mass cytometry

doi: 10.3389/fonc.2022.1016343

Figure Lengend Snippet: Experimental workflow adopted for single-cell mass cytometry analysis. 1. Eight PDHGG primary cell lines were established upon dissociation from tumor patients. After short expansion in stem-cell culture conditions, cells were detached for mass cytometry analysis. The different cell suspensions were barcoded with unique combinations of heavy metal tags and pooled together prior to staining with the selected metal-tag antibodies. 2. The cells were nebulized into a spray of single-cell droplets as they were introduced into the mass cytometer and atomized and ionized by the plasma (ICP). The resulting ion cloud was selected by the quadrupole for heavier reporter masses (>100 Da) which were profiled and quantified on their Time-Of-Flight (TOF). 3. Data were converted to.fcs file and further debarcoded and analyzed in the Cytobank environment, by employing the viSNE tool, and UMAP algorithm.

Article Snippet: Anti-Human CD34 , 149Sm , Fluidigm , 3149013B , AB_2756285.

Techniques: Mass Cytometry, Stem Cell Culture, Staining, Cytometry, Clinical Proteomics

Journal: Frontiers in Oncology

Article Title: Inter and intra-tumor heterogeneity of paediatric type diffuse high-grade gliomas revealed by single-cell mass cytometry

doi: 10.3389/fonc.2022.1016343

Figure Lengend Snippet: CyTOF single-cell analysis of surface antigens in PDHGG patient-derived cell lines. t-SNE maps showing the expression of 10 surface markers (CD31, CD34, CD63, CD36, CD29, CD90, CD140a, CD49c, CD56 and CD61) in each of the eight different PDHGG patient-derived cell lines analyzed through mass cytometry technique. The color gradient refers to the intensity of the expression of the considered marker, in a blue to red scale indicating low and high intensity respectively.

Article Snippet: Anti-Human CD34 , 149Sm , Fluidigm , 3149013B , AB_2756285.

Techniques: Single-cell Analysis, Derivative Assay, Expressing, Mass Cytometry, Marker

Journal: Frontiers in Oncology

Article Title: Inter and intra-tumor heterogeneity of paediatric type diffuse high-grade gliomas revealed by single-cell mass cytometry

doi: 10.3389/fonc.2022.1016343

Figure Lengend Snippet: List of the cell antigens used together with the molecular function and reported expression.

Article Snippet: Anti-Human CD34 , 149Sm , Fluidigm , 3149013B , AB_2756285.

Techniques: Expressing, Migration, Mutagenesis