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cd45 2 147sm  (fluidigm)


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    fluidigm cd45 2 147sm
    a , schematic of Bigh3 −/− transgenic mouse, b, abundance of Bigh3 in the lungs of Bigh3 +/+ and Bigh3 −/− mice, c, representative images of Bigh3 +/+ and Bigh3 −/− and hematoxylin and eosin (top) and Masson’s Trichome stained (bottom) stained lungs, d, whole body weight, e, number of cells and f, number of cells per milligram of tissue in the lungs of Bigh3 +/+ and Bigh3 −/− mice, g, graphical schematic of retro-orbital <t>CD45.2-147Sm</t> antibody injection, h, representative gating of CD45-, CD45+, CD45.2-, and CD45.2+ cells, i, UMAPs of CD45+CD45.2-(top) leukocytes and CD45-stromal cells in the lungs Bigh3 +/+ and Bigh3 −/− mice j, enumeration of leukocyte and stromal cells in the lungs of Bigh3 +/+ and Bigh3 −/− mice, data shown as mean +SEM. All comparisons between Bigh3 −/− and Bigh3 +/+ mice were determined by a student’s t-test (d-f) or multiple unpaired t test (j; only endothelial cells displayed a significant difference between groups). Each symbol represents 1 mouse in b, d-f. Data are from 1 (b) or 2 (d-j) experiments with greater >= 7 mice per group (d-j).
    Cd45 2 147sm, supplied by fluidigm, used in various techniques. Bioz Stars score: 94/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/3147003b/Anti-Mouse+CD45+(30-F11)-147Sm/bio_rxiv__2025__09__02__673852-70-15-17
    Average 94 stars, based on 37 article reviews
    cd45 2 147sm - by Bioz Stars, 2026-09
    94/100 stars

    Images

    1) Product Images from "Bigh3 is essential for pulmonary fibrosis"

    Article Title: Bigh3 is essential for pulmonary fibrosis

    Journal: bioRxiv

    doi: 10.1101/2025.09.02.673852

    a , schematic of Bigh3 −/− transgenic mouse, b, abundance of Bigh3 in the lungs of Bigh3 +/+ and Bigh3 −/− mice, c, representative images of Bigh3 +/+ and Bigh3 −/− and hematoxylin and eosin (top) and Masson’s Trichome stained (bottom) stained lungs, d, whole body weight, e, number of cells and f, number of cells per milligram of tissue in the lungs of Bigh3 +/+ and Bigh3 −/− mice, g, graphical schematic of retro-orbital CD45.2-147Sm antibody injection, h, representative gating of CD45-, CD45+, CD45.2-, and CD45.2+ cells, i, UMAPs of CD45+CD45.2-(top) leukocytes and CD45-stromal cells in the lungs Bigh3 +/+ and Bigh3 −/− mice j, enumeration of leukocyte and stromal cells in the lungs of Bigh3 +/+ and Bigh3 −/− mice, data shown as mean +SEM. All comparisons between Bigh3 −/− and Bigh3 +/+ mice were determined by a student’s t-test (d-f) or multiple unpaired t test (j; only endothelial cells displayed a significant difference between groups). Each symbol represents 1 mouse in b, d-f. Data are from 1 (b) or 2 (d-j) experiments with greater >= 7 mice per group (d-j).
    Figure Legend Snippet: a , schematic of Bigh3 −/− transgenic mouse, b, abundance of Bigh3 in the lungs of Bigh3 +/+ and Bigh3 −/− mice, c, representative images of Bigh3 +/+ and Bigh3 −/− and hematoxylin and eosin (top) and Masson’s Trichome stained (bottom) stained lungs, d, whole body weight, e, number of cells and f, number of cells per milligram of tissue in the lungs of Bigh3 +/+ and Bigh3 −/− mice, g, graphical schematic of retro-orbital CD45.2-147Sm antibody injection, h, representative gating of CD45-, CD45+, CD45.2-, and CD45.2+ cells, i, UMAPs of CD45+CD45.2-(top) leukocytes and CD45-stromal cells in the lungs Bigh3 +/+ and Bigh3 −/− mice j, enumeration of leukocyte and stromal cells in the lungs of Bigh3 +/+ and Bigh3 −/− mice, data shown as mean +SEM. All comparisons between Bigh3 −/− and Bigh3 +/+ mice were determined by a student’s t-test (d-f) or multiple unpaired t test (j; only endothelial cells displayed a significant difference between groups). Each symbol represents 1 mouse in b, d-f. Data are from 1 (b) or 2 (d-j) experiments with greater >= 7 mice per group (d-j).

    Techniques Used: Transgenic Assay, Staining, Injection

    Related Articles

    other:


    Article Title: COX2 regulates senescence secretome composition and senescence surveillance through PGE 2
    Article Snippet: Rat anti-CD45-147Sm , Fluidigm , # 3147003B, RRID: AB_2811243.

    Article Title: High-resolution phenotyping of early acute rejection reveals a conserved alloimmune signature
    Article Snippet: CD45 (30-F11) , Fluidigm , Cat3147003B; Lot#3181713.

    Immunopeptidomics:

    Article Title: High-resolution phenotyping of early acute rejection reveals a conserved alloimmune signature.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Gr1 (RB6-8C5); Clone preferentially binds Ly6G Fluidigm Cat#3141005B; Lot#1201813 CD11c (N418) Fluidigm Cat#3142003B; Lot#1201819 GITR (DTA1) Fluidigm Cat#3143019B; Lot#2171502 MHCI (28-14-8) Fluidigm Cat#3144016B; Lot#0151802 CD69 (H1.2F3) Fluidigm Cat#3145005B; Lot#3511606 CD5 (53-7.3) Fluidigm Cat#3146012B; Lot#2041519 CD45 (30-F11) Fluidigm Cat3147003B; Lot#3181713 CD11b (M1/70); Alt. name: Mac-1 Fluidigm Cat#3148003B; Lot#1621803 CD19 (6D5) Fluidigm Cat#3149002B; Lot#0581828 CD24 (M1/69) Fluidigm Cat#3150009B; Lot#0901508 CD25 (3C7) Fluidigm Cat#3151007B; Lot#3511605 CD3ε (145 2X11) Fluidigm Cat#3152004B; Lot#2601606 CD8a (53 6.7) Fluidigm Cat#3153012B; Lot#0101806 CTLA4 (UC10-4B9); Alt. name: CD152 Fluidigm Cat#3154008B; Lot#1841809 TCRgd (UC7-13D5) Biolegend Cat#107502 CCR6 (29-2L17); Alt. name: CD196 Fluidigm Cat#3156016A; Lot#0581825 CD80 (16-10A1); Alt. name: B7-1 Biolegend Cat#104702 FOXP3 (FJK-16 s) Fluidigm Cat#3158003A; Lot#0691401 F4/80 (BM8) Fluidigm Cat#3159009B; Lot#0541707 CD62L (MEL-14) Fluidigm Cat#3160008B; Lot#2631805 Ly6C (HK1.4) Fluidigm Cat#3162014B; Lot#2341706 CCR7 (4B12); Alt. name: CD197 Fluidigm Cat#3164013A; Lot#0901709 NK1.1 (PK136) Fluidigm Cat#3165018B; Lot#0101808 CD27 (LG.3A10) Biolegend Cat#124202 NKp46 (29A1.4); Alt. name: CD335 Fluidigm Cat#3167008B; Lot#1501808 CD21 (7G6) Fluidigm Cat#3168010B; Lot#2371409 TCRb (H57 597) Fluidigm Cat#3169002B; Lot#0761712 CD40L (MR1); Alt. name: CD154 Fluidigm Cat#3170011B; Lot#2041508 CD44 (IM7) Fluidigm Cat#3171003B; Lot#3421608 CD4 (RM4-5) Fluidigm Cat#3172003B; Lot#1201808 Granzyme B (GB11) Fluidigm Cat#3173006B; Lot#1511802 LAG3 (C9B7W) Fluidigm Cat#3174019B; Lot#1201814 CD127 (A7R34); Alt. name: IL7Ra Fluidigm Cat#3175006B; Lot#0101807 B220 (RA3-6B2); Alt. name: CD45R Fluidigm Cat#3176002B; Lot#0321704 MHCII I-A/I-E (M5/114.15.2); Binds both donor and recipient MHC Fluidigm Cat#3209006B; Lot#2851707 Fc Block: TruStain FcX (anti-mouse CD16/32) Antibody Biolegend Cat#101320 Critical commercial assays Dead cell removal kit StemCell Cat#17899 40 micron nylon mesh Fisher Cat#352340 Cisplatin Fluidigm Cat#201195 Cell staining buffer Fluidigm Cat#201068 Cell-ID 20-Plex Pd Barcoding Kit Fluidigm Cat#201060 (Continued on next page) e1 Cell Reports 34, 108806, March 2, 2021 .. REAGENT or RESOURCE SOURCE IDENTIFIER Fix I buffer Fluidigm Cat#201065 Perm-S buffer Fluidigm Cat#201066 Fix and perm buffer Fluidigm Cat#201067 DNA intercalator Fluidigm Cat#201192a 35 micron cell strainer Fisher Cat#08-771-23 Experimental models: organisms/strains Mouse: C57BL/6 (H-2Kb) The Jackson Laboratory JAX: 000664 Mouse: BALB/c (H-2Kd) The Jackson Laboratory JAX: 000651 Software and algorithms Premessa Parker Institute for Cancer Immunotherapy https://github.com/ParkerICI/premessa FlowSOM (v1.18.0) Van Gassen et al., 2020 https://bioconductor.org/packages/release/bioc/ html/FlowSOM.html ConsensusClusterPlus (v1.50.0) Wilkerson and Hayes, 2010 https://bioconductor.org/packages/release/bioc/ html/ConsensusClusterPlus.html UMAP; v0.3.2.1 Becht et al., 2018 https://github.com/lmcinnes/umap Rtsne; v0.15 van der Maaten and Hinton, 2008 https://github.com/jkrijthe/Rtsne

    Blocking Assay:

    Article Title: High-resolution phenotyping of early acute rejection reveals a conserved alloimmune signature.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Gr1 (RB6-8C5); Clone preferentially binds Ly6G Fluidigm Cat#3141005B; Lot#1201813 CD11c (N418) Fluidigm Cat#3142003B; Lot#1201819 GITR (DTA1) Fluidigm Cat#3143019B; Lot#2171502 MHCI (28-14-8) Fluidigm Cat#3144016B; Lot#0151802 CD69 (H1.2F3) Fluidigm Cat#3145005B; Lot#3511606 CD5 (53-7.3) Fluidigm Cat#3146012B; Lot#2041519 CD45 (30-F11) Fluidigm Cat3147003B; Lot#3181713 CD11b (M1/70); Alt. name: Mac-1 Fluidigm Cat#3148003B; Lot#1621803 CD19 (6D5) Fluidigm Cat#3149002B; Lot#0581828 CD24 (M1/69) Fluidigm Cat#3150009B; Lot#0901508 CD25 (3C7) Fluidigm Cat#3151007B; Lot#3511605 CD3ε (145 2X11) Fluidigm Cat#3152004B; Lot#2601606 CD8a (53 6.7) Fluidigm Cat#3153012B; Lot#0101806 CTLA4 (UC10-4B9); Alt. name: CD152 Fluidigm Cat#3154008B; Lot#1841809 TCRgd (UC7-13D5) Biolegend Cat#107502 CCR6 (29-2L17); Alt. name: CD196 Fluidigm Cat#3156016A; Lot#0581825 CD80 (16-10A1); Alt. name: B7-1 Biolegend Cat#104702 FOXP3 (FJK-16 s) Fluidigm Cat#3158003A; Lot#0691401 F4/80 (BM8) Fluidigm Cat#3159009B; Lot#0541707 CD62L (MEL-14) Fluidigm Cat#3160008B; Lot#2631805 Ly6C (HK1.4) Fluidigm Cat#3162014B; Lot#2341706 CCR7 (4B12); Alt. name: CD197 Fluidigm Cat#3164013A; Lot#0901709 NK1.1 (PK136) Fluidigm Cat#3165018B; Lot#0101808 CD27 (LG.3A10) Biolegend Cat#124202 NKp46 (29A1.4); Alt. name: CD335 Fluidigm Cat#3167008B; Lot#1501808 CD21 (7G6) Fluidigm Cat#3168010B; Lot#2371409 TCRb (H57 597) Fluidigm Cat#3169002B; Lot#0761712 CD40L (MR1); Alt. name: CD154 Fluidigm Cat#3170011B; Lot#2041508 CD44 (IM7) Fluidigm Cat#3171003B; Lot#3421608 CD4 (RM4-5) Fluidigm Cat#3172003B; Lot#1201808 Granzyme B (GB11) Fluidigm Cat#3173006B; Lot#1511802 LAG3 (C9B7W) Fluidigm Cat#3174019B; Lot#1201814 CD127 (A7R34); Alt. name: IL7Ra Fluidigm Cat#3175006B; Lot#0101807 B220 (RA3-6B2); Alt. name: CD45R Fluidigm Cat#3176002B; Lot#0321704 MHCII I-A/I-E (M5/114.15.2); Binds both donor and recipient MHC Fluidigm Cat#3209006B; Lot#2851707 Fc Block: TruStain FcX (anti-mouse CD16/32) Antibody Biolegend Cat#101320 Critical commercial assays Dead cell removal kit StemCell Cat#17899 40 micron nylon mesh Fisher Cat#352340 Cisplatin Fluidigm Cat#201195 Cell staining buffer Fluidigm Cat#201068 Cell-ID 20-Plex Pd Barcoding Kit Fluidigm Cat#201060 (Continued on next page) e1 Cell Reports 34, 108806, March 2, 2021 .. REAGENT or RESOURCE SOURCE IDENTIFIER Fix I buffer Fluidigm Cat#201065 Perm-S buffer Fluidigm Cat#201066 Fix and perm buffer Fluidigm Cat#201067 DNA intercalator Fluidigm Cat#201192a 35 micron cell strainer Fisher Cat#08-771-23 Experimental models: organisms/strains Mouse: C57BL/6 (H-2Kb) The Jackson Laboratory JAX: 000664 Mouse: BALB/c (H-2Kd) The Jackson Laboratory JAX: 000651 Software and algorithms Premessa Parker Institute for Cancer Immunotherapy https://github.com/ParkerICI/premessa FlowSOM (v1.18.0) Van Gassen et al., 2020 https://bioconductor.org/packages/release/bioc/ html/FlowSOM.html ConsensusClusterPlus (v1.50.0) Wilkerson and Hayes, 2010 https://bioconductor.org/packages/release/bioc/ html/ConsensusClusterPlus.html UMAP; v0.3.2.1 Becht et al., 2018 https://github.com/lmcinnes/umap Rtsne; v0.15 van der Maaten and Hinton, 2008 https://github.com/jkrijthe/Rtsne

    Staining:

    Article Title: High-resolution phenotyping of early acute rejection reveals a conserved alloimmune signature.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Gr1 (RB6-8C5); Clone preferentially binds Ly6G Fluidigm Cat#3141005B; Lot#1201813 CD11c (N418) Fluidigm Cat#3142003B; Lot#1201819 GITR (DTA1) Fluidigm Cat#3143019B; Lot#2171502 MHCI (28-14-8) Fluidigm Cat#3144016B; Lot#0151802 CD69 (H1.2F3) Fluidigm Cat#3145005B; Lot#3511606 CD5 (53-7.3) Fluidigm Cat#3146012B; Lot#2041519 CD45 (30-F11) Fluidigm Cat3147003B; Lot#3181713 CD11b (M1/70); Alt. name: Mac-1 Fluidigm Cat#3148003B; Lot#1621803 CD19 (6D5) Fluidigm Cat#3149002B; Lot#0581828 CD24 (M1/69) Fluidigm Cat#3150009B; Lot#0901508 CD25 (3C7) Fluidigm Cat#3151007B; Lot#3511605 CD3ε (145 2X11) Fluidigm Cat#3152004B; Lot#2601606 CD8a (53 6.7) Fluidigm Cat#3153012B; Lot#0101806 CTLA4 (UC10-4B9); Alt. name: CD152 Fluidigm Cat#3154008B; Lot#1841809 TCRgd (UC7-13D5) Biolegend Cat#107502 CCR6 (29-2L17); Alt. name: CD196 Fluidigm Cat#3156016A; Lot#0581825 CD80 (16-10A1); Alt. name: B7-1 Biolegend Cat#104702 FOXP3 (FJK-16 s) Fluidigm Cat#3158003A; Lot#0691401 F4/80 (BM8) Fluidigm Cat#3159009B; Lot#0541707 CD62L (MEL-14) Fluidigm Cat#3160008B; Lot#2631805 Ly6C (HK1.4) Fluidigm Cat#3162014B; Lot#2341706 CCR7 (4B12); Alt. name: CD197 Fluidigm Cat#3164013A; Lot#0901709 NK1.1 (PK136) Fluidigm Cat#3165018B; Lot#0101808 CD27 (LG.3A10) Biolegend Cat#124202 NKp46 (29A1.4); Alt. name: CD335 Fluidigm Cat#3167008B; Lot#1501808 CD21 (7G6) Fluidigm Cat#3168010B; Lot#2371409 TCRb (H57 597) Fluidigm Cat#3169002B; Lot#0761712 CD40L (MR1); Alt. name: CD154 Fluidigm Cat#3170011B; Lot#2041508 CD44 (IM7) Fluidigm Cat#3171003B; Lot#3421608 CD4 (RM4-5) Fluidigm Cat#3172003B; Lot#1201808 Granzyme B (GB11) Fluidigm Cat#3173006B; Lot#1511802 LAG3 (C9B7W) Fluidigm Cat#3174019B; Lot#1201814 CD127 (A7R34); Alt. name: IL7Ra Fluidigm Cat#3175006B; Lot#0101807 B220 (RA3-6B2); Alt. name: CD45R Fluidigm Cat#3176002B; Lot#0321704 MHCII I-A/I-E (M5/114.15.2); Binds both donor and recipient MHC Fluidigm Cat#3209006B; Lot#2851707 Fc Block: TruStain FcX (anti-mouse CD16/32) Antibody Biolegend Cat#101320 Critical commercial assays Dead cell removal kit StemCell Cat#17899 40 micron nylon mesh Fisher Cat#352340 Cisplatin Fluidigm Cat#201195 Cell staining buffer Fluidigm Cat#201068 Cell-ID 20-Plex Pd Barcoding Kit Fluidigm Cat#201060 (Continued on next page) e1 Cell Reports 34, 108806, March 2, 2021 .. REAGENT or RESOURCE SOURCE IDENTIFIER Fix I buffer Fluidigm Cat#201065 Perm-S buffer Fluidigm Cat#201066 Fix and perm buffer Fluidigm Cat#201067 DNA intercalator Fluidigm Cat#201192a 35 micron cell strainer Fisher Cat#08-771-23 Experimental models: organisms/strains Mouse: C57BL/6 (H-2Kb) The Jackson Laboratory JAX: 000664 Mouse: BALB/c (H-2Kd) The Jackson Laboratory JAX: 000651 Software and algorithms Premessa Parker Institute for Cancer Immunotherapy https://github.com/ParkerICI/premessa FlowSOM (v1.18.0) Van Gassen et al., 2020 https://bioconductor.org/packages/release/bioc/ html/FlowSOM.html ConsensusClusterPlus (v1.50.0) Wilkerson and Hayes, 2010 https://bioconductor.org/packages/release/bioc/ html/ConsensusClusterPlus.html UMAP; v0.3.2.1 Becht et al., 2018 https://github.com/lmcinnes/umap Rtsne; v0.15 van der Maaten and Hinton, 2008 https://github.com/jkrijthe/Rtsne



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    https://www.bioz.com/product/3147003b/Anti-Mouse+CD45+(30-F11)-147Sm/pm36163264__41375_2022_1682_MOESM1_ESM-118-169-172
    Average 94 stars, based on 1 article reviews
    168 er cd45 fluidigm 3147003b 30 f11 147 sm cd4 fluidigm 3172003b rm45 172 yb cytoplasmic - by Bioz Stars, 2026-09
    94/100 stars
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    3147003c  (fluidigm)
    94
    fluidigm 3147003c

    3147003c, supplied by fluidigm, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/3147003b/Anti-Mouse+CD45+(30-F11)-147Sm/pm35947948-388-180-178
    Average 94 stars, based on 1 article reviews
    3147003c - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    a , schematic of Bigh3 −/− transgenic mouse, b, abundance of Bigh3 in the lungs of Bigh3 +/+ and Bigh3 −/− mice, c, representative images of Bigh3 +/+ and Bigh3 −/− and hematoxylin and eosin (top) and Masson’s Trichome stained (bottom) stained lungs, d, whole body weight, e, number of cells and f, number of cells per milligram of tissue in the lungs of Bigh3 +/+ and Bigh3 −/− mice, g, graphical schematic of retro-orbital CD45.2-147Sm antibody injection, h, representative gating of CD45-, CD45+, CD45.2-, and CD45.2+ cells, i, UMAPs of CD45+CD45.2-(top) leukocytes and CD45-stromal cells in the lungs Bigh3 +/+ and Bigh3 −/− mice j, enumeration of leukocyte and stromal cells in the lungs of Bigh3 +/+ and Bigh3 −/− mice, data shown as mean +SEM. All comparisons between Bigh3 −/− and Bigh3 +/+ mice were determined by a student’s t-test (d-f) or multiple unpaired t test (j; only endothelial cells displayed a significant difference between groups). Each symbol represents 1 mouse in b, d-f. Data are from 1 (b) or 2 (d-j) experiments with greater >= 7 mice per group (d-j).

    Journal: bioRxiv

    Article Title: Bigh3 is essential for pulmonary fibrosis

    doi: 10.1101/2025.09.02.673852

    Figure Lengend Snippet: a , schematic of Bigh3 −/− transgenic mouse, b, abundance of Bigh3 in the lungs of Bigh3 +/+ and Bigh3 −/− mice, c, representative images of Bigh3 +/+ and Bigh3 −/− and hematoxylin and eosin (top) and Masson’s Trichome stained (bottom) stained lungs, d, whole body weight, e, number of cells and f, number of cells per milligram of tissue in the lungs of Bigh3 +/+ and Bigh3 −/− mice, g, graphical schematic of retro-orbital CD45.2-147Sm antibody injection, h, representative gating of CD45-, CD45+, CD45.2-, and CD45.2+ cells, i, UMAPs of CD45+CD45.2-(top) leukocytes and CD45-stromal cells in the lungs Bigh3 +/+ and Bigh3 −/− mice j, enumeration of leukocyte and stromal cells in the lungs of Bigh3 +/+ and Bigh3 −/− mice, data shown as mean +SEM. All comparisons between Bigh3 −/− and Bigh3 +/+ mice were determined by a student’s t-test (d-f) or multiple unpaired t test (j; only endothelial cells displayed a significant difference between groups). Each symbol represents 1 mouse in b, d-f. Data are from 1 (b) or 2 (d-j) experiments with greater >= 7 mice per group (d-j).

    Article Snippet: Animals were injected retro-orbitally using an insulin syringe (BD; NJ, USA; Cat. 305932) with 3ug CD45.2 147Sm (Standard Biotools; ON, CAN; Cat. 3147003B) in 100-125uL of sterile saline.

    Techniques: Transgenic Assay, Staining, Injection

    Journal: Cell reports

    Article Title: AXL-initiated paracrine activation of pSTAT3 enhances mesenchymal and vasculogenic supportive features of tumor-associated macrophages

    doi: 10.1016/j.celrep.2023.113067

    Figure Lengend Snippet:

    Article Snippet: Anti-Mouse CD45 147Sm , Fluidigm , Cat#3147003B; RRID: AB_2811243.

    Techniques: Recombinant, Reverse Transcription, Cell Viability Assay, In Situ, SYBR Green Assay, RNA Sequencing, Quantitative RT-PCR, Luciferase, Software