Clinical Proteomics:
Recombinant:
Staining:
Software:
other:
Binding Assay:Article Title: Multimodal analysis of dysregulated heme metabolism, hypoxic signaling, and stress erythropoiesis in Down syndrome.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Fc Receptor Binding Inhibitor polyclonal eBioscience/ThermoFisher Scientific Cat # 14-9161-73; RRID: AB_468582 Mouse monoclonal Anti-Human CD45 (Clone HI30) Fluidigm Cat # 3089003B; RRID: AB_2661851 Mouse monoclonal anti-human CD66a/c/d/e (clone B1.1/CD66) BD Biosciences Cat # 551354; RRID: AB_394166 Mouse monoclonal anti-human Cleaved PARP (Clone F21-852) BD Biosciences Cat # 552596, RRID: AB_394437 Mouse monoclonal anti-human RORgt (Clone RORg2) BioLegend Cat # 646502, RRID: AB_2238503 Mouse monoclonal anti-human IgM (clone MHM-88) BioLegend Cat # 314502; RRID: AB_493003 Mouse monoclonal anti-human PICP (Clone PCIDG10) Millipore Cat # MAB1913, RRID: AB_94406 Mouse monoclonal anti-human CD19 (clone HIP19) Fluidigm Cat # 3142001; RRID: AB_2651155 Mouse monoclonal anti-human CD123 (clone 6H6) Fluidigm Cat # 3143014B, RRID: AB_2811081 Mouse monoclonal anti-human CD11b (Clone ICRF44) Fluidigm Cat # 3144001, RRID: AB_2714152 Mouse monoclonal anti-human CD4 (clone RPA-T4) Fluidigm Cat # 3145001; RRID: AB_2661789 Mouse monoclonal anti-human IgD (clone IA6-2) Fluidigm Cat # 3146005B; RRID: AB_2811082 Mouse monoclonal anti-human CD11c (clone Bu15) Fluidigm Cat # 3147008; RRID: AB_2687850 Mouse monoclonal anti-human TCR Va7.2 (Clone 3C10) Biolegend Cat # 351702, RRID: AB_10900258 Mouse monoclonal anti-human CD127 (clone A019D5) Fluidigm Cat # 3149011; RRID: AB_2661792 Mouse monoclonal anti-human CD1c (clone L161) Biolegend Cat # 331501; RRID: AB_1088996 Mouse monoclonal anti-human CD14 (clone M5E2) Fluidigm Cat # 3151009B, RRID: AB_2810244 Mouse monoclonal anti-human TCRgd (Clone 11F2) Fluidigm Cat # 3152008B, RRID: AB_2687643 Mouse monoclonal anti-human CD7 (clone CD7-6B7) DVS Sciences Cat # 3153014B; RRID: AB_2811084 Mouse monoclonal anti-human CD3 (clone UCHT1) DVS Sciences Cat # 3154003B; RRID: AB_2811086 Mouse monoclonal anti-human CD279/PD1 (clone EH12.2H7) Fluidigm Cat # 3155009B; RRID: AB_2811087 Mouse monoclonal anti-human PD-L1 (clone 29E.2A3) Fluidigm Cat # 3156026, RRID: AB_2687855 Mouse monoclonal anti-human CD45RA (clone HI100) BioLegend Cat # 304102; RRID: AB_314406 Mouse monoclonal anti-human EMR1 (Clone BM8) Biolegend Cat # 123102, RRID: AB_893506 Mouse monoclonal anti-human FOXP3 (clone 259D/C7) Fluidigm Cat # 3159028A; RRID: AB_2811088 (Continued on next page) 16 Cell Reports 43, 114599, August 27, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Mouse monoclonal anti-human T-bet (Clone 4B10) Fluidigm Cat # 3160010B, RRID: AB_2810251 Mouse monoclonal anti-human CD16 (clone B73.1) Biolegend Cat # 360702; RRID: AB_2562693 Mouse monoclonal anti-human CD8a (clone RPA-T8) Fluidigm Cat # 3162015; RRID: AB_2661802 Mouse monoclonal anti-human CD34 (clone 581) Fluidigm Cat # 3163014B; RRID: AB_2811091 Mouse monoclonal anti-human CD45RO (clone UCHL1) Fluidigm Cat # 3164007B; RRID: AB_2811092 Mouse monoclonal anti-human CD15 (Clone W6D3) Biolegend Cat # 323002, RRID: AB_756008 Rabbit monoclonal anti-human phospho4E-BP1 (Thr37/Thr46) (clone 236B4) Cell Signaling Technology Cat # 2855; RRID: AB_560835 Mouse monoclonal anti-human CD27 (clone L128) Fluidigm Cat # 3167006B; RRID: AB_2811093 Rabbit monoclonal anti-human phospho-STAT1 (Tyr701) (clone 58D6) Cell Signaling Technology Cat # 9167; RRID: AB_561284 Mouse monoclonal anti-human CD25 (clone 2A3) Fluidigm Cat # 3169003; RRID: AB_2661806 Mouse monoclonal anti-human CD33 (clone WM53) Biolegend Cat # 303402, RRID: AB_314346 Mouse monoclonal anti-human CD95 (clone DX2) Biolegend Cat # 305602, RRID: AB_314540 Mouse monoclonal anti-human CD38 (clone HIT2) Fluidigm Cat # 3172007B; RRID: AB_2756288 Mouse monoclonal anti-human GZMB (clone GB11) Fluidigm Cat # 3173006B; RRID: AB_2811095 Mouse monoclonal anti-human HLA-DR (clone L243) Fluidigm Cat # 3174001; RRID: AB_2665397 Mouse monoclonal anti-human CD161 (clone DX12) BD Biosciences Cat # 556079, RRID: AB_396346 Mouse monoclonal anti-human CD56 (clone N901) Fluidigm Cat # 3176009B; RRID: AB_2811096 Critical commercial assays Vacutainer EDTA tubes BD Cat # 36643 PAXgene Blood RNA tubes Qiagen Cat # 762165 PAXgene Blood RNA Kit Qiagen Cat # 762164 Allprep DNA/RNA/miRNA Universal Kit Qiagen Cat # 80224 GlobinClear kit ThermoFisher Scientific Cat # AM1980 NEBNext Poly(A) mRNA Magnetic Isolation Module New England Biolabs Cat #E7490 NEBNext Ultra II Directional RNA Library Prep Kit for Illumina New England Biolabs Cat #E7760; V-PLEX Human Biomarker 54-Plex MesoScale Discovery Cat #K15248D Transcription Factor Phospho Buffer Set BD Pharmingen Cat # 563239 10X PBS, pH 7.2 Rockland Cat # MB-008 Cell Staining Buffer Fluidigm Cat # 201068 Cell-IDTM 20- Plex Pd Barcoding Kit Fluidigm Cat # PRD023 Cell-ID Intercalator-Ir Fluidigm Cat # 201192A Maxpar Antibody Labeling Kit Fluidigm Cat # 201160B (Continued on next page) Cell Reports 43, 114599, August 27, 2024 17
Recombinase Polymerase Amplification:Article Title: Multimodal analysis of dysregulated heme metabolism, hypoxic signaling, and stress erythropoiesis in Down syndrome.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Fc Receptor Binding Inhibitor polyclonal eBioscience/ThermoFisher Scientific Cat # 14-9161-73; RRID: AB_468582 Mouse monoclonal Anti-Human CD45 (Clone HI30) Fluidigm Cat # 3089003B; RRID: AB_2661851 Mouse monoclonal anti-human CD66a/c/d/e (clone B1.1/CD66) BD Biosciences Cat # 551354; RRID: AB_394166 Mouse monoclonal anti-human Cleaved PARP (Clone F21-852) BD Biosciences Cat # 552596, RRID: AB_394437 Mouse monoclonal anti-human RORgt (Clone RORg2) BioLegend Cat # 646502, RRID: AB_2238503 Mouse monoclonal anti-human IgM (clone MHM-88) BioLegend Cat # 314502; RRID: AB_493003 Mouse monoclonal anti-human PICP (Clone PCIDG10) Millipore Cat # MAB1913, RRID: AB_94406 Mouse monoclonal anti-human CD19 (clone HIP19) Fluidigm Cat # 3142001; RRID: AB_2651155 Mouse monoclonal anti-human CD123 (clone 6H6) Fluidigm Cat # 3143014B, RRID: AB_2811081 Mouse monoclonal anti-human CD11b (Clone ICRF44) Fluidigm Cat # 3144001, RRID: AB_2714152 Mouse monoclonal anti-human CD4 (clone RPA-T4) Fluidigm Cat # 3145001; RRID: AB_2661789 Mouse monoclonal anti-human IgD (clone IA6-2) Fluidigm Cat # 3146005B; RRID: AB_2811082 Mouse monoclonal anti-human CD11c (clone Bu15) Fluidigm Cat # 3147008; RRID: AB_2687850 Mouse monoclonal anti-human TCR Va7.2 (Clone 3C10) Biolegend Cat # 351702, RRID: AB_10900258 Mouse monoclonal anti-human CD127 (clone A019D5) Fluidigm Cat # 3149011; RRID: AB_2661792 Mouse monoclonal anti-human CD1c (clone L161) Biolegend Cat # 331501; RRID: AB_1088996 Mouse monoclonal anti-human CD14 (clone M5E2) Fluidigm Cat # 3151009B, RRID: AB_2810244 Mouse monoclonal anti-human TCRgd (Clone 11F2) Fluidigm Cat # 3152008B, RRID: AB_2687643 Mouse monoclonal anti-human CD7 (clone CD7-6B7) DVS Sciences Cat # 3153014B; RRID: AB_2811084 Mouse monoclonal anti-human CD3 (clone UCHT1) DVS Sciences Cat # 3154003B; RRID: AB_2811086 Mouse monoclonal anti-human CD279/PD1 (clone EH12.2H7) Fluidigm Cat # 3155009B; RRID: AB_2811087 Mouse monoclonal anti-human PD-L1 (clone 29E.2A3) Fluidigm Cat # 3156026, RRID: AB_2687855 Mouse monoclonal anti-human CD45RA (clone HI100) BioLegend Cat # 304102; RRID: AB_314406 Mouse monoclonal anti-human EMR1 (Clone BM8) Biolegend Cat # 123102, RRID: AB_893506 Mouse monoclonal anti-human FOXP3 (clone 259D/C7) Fluidigm Cat # 3159028A; RRID: AB_2811088 (Continued on next page) 16 Cell Reports 43, 114599, August 27, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Mouse monoclonal anti-human T-bet (Clone 4B10) Fluidigm Cat # 3160010B, RRID: AB_2810251 Mouse monoclonal anti-human CD16 (clone B73.1) Biolegend Cat # 360702; RRID: AB_2562693 Mouse monoclonal anti-human CD8a (clone RPA-T8) Fluidigm Cat # 3162015; RRID: AB_2661802 Mouse monoclonal anti-human CD34 (clone 581) Fluidigm Cat # 3163014B; RRID: AB_2811091 Mouse monoclonal anti-human CD45RO (clone UCHL1) Fluidigm Cat # 3164007B; RRID: AB_2811092 Mouse monoclonal anti-human CD15 (Clone W6D3) Biolegend Cat # 323002, RRID: AB_756008 Rabbit monoclonal anti-human phospho4E-BP1 (Thr37/Thr46) (clone 236B4) Cell Signaling Technology Cat # 2855; RRID: AB_560835 Mouse monoclonal anti-human CD27 (clone L128) Fluidigm Cat # 3167006B; RRID: AB_2811093 Rabbit monoclonal anti-human phospho-STAT1 (Tyr701) (clone 58D6) Cell Signaling Technology Cat # 9167; RRID: AB_561284 Mouse monoclonal anti-human CD25 (clone 2A3) Fluidigm Cat # 3169003; RRID: AB_2661806 Mouse monoclonal anti-human CD33 (clone WM53) Biolegend Cat # 303402, RRID: AB_314346 Mouse monoclonal anti-human CD95 (clone DX2) Biolegend Cat # 305602, RRID: AB_314540 Mouse monoclonal anti-human CD38 (clone HIT2) Fluidigm Cat # 3172007B; RRID: AB_2756288 Mouse monoclonal anti-human GZMB (clone GB11) Fluidigm Cat # 3173006B; RRID: AB_2811095 Mouse monoclonal anti-human HLA-DR (clone L243) Fluidigm Cat # 3174001; RRID: AB_2665397 Mouse monoclonal anti-human CD161 (clone DX12) BD Biosciences Cat # 556079, RRID: AB_396346 Mouse monoclonal anti-human CD56 (clone N901) Fluidigm Cat # 3176009B; RRID: AB_2811096 Critical commercial assays Vacutainer EDTA tubes BD Cat # 36643 PAXgene Blood RNA tubes Qiagen Cat # 762165 PAXgene Blood RNA Kit Qiagen Cat # 762164 Allprep DNA/RNA/miRNA Universal Kit Qiagen Cat # 80224 GlobinClear kit ThermoFisher Scientific Cat # AM1980 NEBNext Poly(A) mRNA Magnetic Isolation Module New England Biolabs Cat #E7490 NEBNext Ultra II Directional RNA Library Prep Kit for Illumina New England Biolabs Cat #E7760; V-PLEX Human Biomarker 54-Plex MesoScale Discovery Cat #K15248D Transcription Factor Phospho Buffer Set BD Pharmingen Cat # 563239 10X PBS, pH 7.2 Rockland Cat # MB-008 Cell Staining Buffer Fluidigm Cat # 201068 Cell-IDTM 20- Plex Pd Barcoding Kit Fluidigm Cat # PRD023 Cell-ID Intercalator-Ir Fluidigm Cat # 201192A Maxpar Antibody Labeling Kit Fluidigm Cat # 201160B (Continued on next page) Cell Reports 43, 114599, August 27, 2024 17
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