146nd cytof standard biotools 3146003b (fluidigm)
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146nd cytof standard biotools 3146003b
146nd Cytof Standard Biotools 3146003b, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/3146003b/Anti-Mouse+CD8a+(53-6%2E7)-146Nd/pmc10182775__JEM_20220509_TableS1-1-42-44
Average 93 stars, based on 8 article reviews
146nd Cytof Standard Biotools 3146003b, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/3146003b/Anti-Mouse+CD8a+(53-6%2E7)-146Nd/pmc10182775__JEM_20220509_TableS1-1-42-44
Average 93 stars, based on 8 article reviews
146nd cytof standard biotools 3146003b - by Bioz Stars,
2026-09
93/100 stars
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Recombinase Polymerase Amplification:Article Title: TSG-6+ cancer-associated fibroblasts modulate myeloid cell responses and impair anti-tumor response to immune checkpoint therapy in pancreatic cancer Article Snippet: 4) The antibodies used for murine immunofluorescence are: CD44 (Cell Signaling Technologies, clone CST37279, 1:300 dilution), CD68 (Abcam, clone ab125212, 1:200 dilution). .. 5) The antibodies used for CyTOF have been described in Supplementary Table 2 and below: Antibodies Source Identifier Dilution Anti-CD45 (clone 30-F11) Standard BioTools Cat# 3089005B 1:200 Anti-I-A/I-E (MHCII) (clone M5/114.15.2 ) Biolegend Cat# 107637 1:800 Anti-CD4 (clone RMP4-5) Biolegend Cat# 100506 1:200 Anti-CD64 (clone X54-5/7.1) Biolegend Cat# 139302 1:50 Anti-Ly6G (clone RPA-T4) Biolegend Cat# 127620 1:200 Anti-Tbet (clone 4B10) Biolegend Cat# 644805 1:75 Anti-VISTA (clone MIH63) Biolegend Cat# 150202 1:200 Anti-TNFa (clone MP6-XT22) Standard BioTools Cat# 3162002B 1:50 Anti-PD-L2 (clone Ty25) Biolegend Cat# 107202 1:100 Anti-CD103 (clone 2E7) Biolegend Cat# 121402 1:200 Anti-IFNG (clone XMG1.2) Biolegend Cat# 505802 1:25 Anti-Eomes (clone Dan11mag) eBioscience Cat# 14-4875-82 1:50 Anti-CD80 (clone 16-10A1) Biolegend Cat# 104710 1:800 Anti-CD163 (clone EPR19518) Abcam Cat# ab182422 1:50 Anti-GATA3 (clone TWAJ) eBioscience Cat# 14-9966-82 1:100 Anti-ICOS (clone 7E.17G9) eBioscience Cat# 14-9942-85 1:100 Anti-F4/80 (clone BM8) Biolegend Cat# 123143 1:100 Anti-CD86 (clone GL-1) Biolegend Cat# 105002 1:100 Anti-GranzymeB (clone QA16A02) Biolegend Cat# 372202 1:100 Anti-CD115 (clone AFS98) Standard BioTools Cat# 3144012B 1:50 Anti-FoxP3 (clone FJK-16s) Standard BioTools Cat# 3158003A 1:100 Anti- CD8a (clone 53-6.7) Standard BioTools Cat# Article Title: TSG-6+ cancer-associated fibroblasts modulate myeloid cell responses and impair anti-tumor response to immune checkpoint therapy in pancreatic cancer Article Snippet: .. Supplementary Table 2 Antibodies for murine CyTOF Source Identifier Dilution Anti-CD45 (clone 30-F11) Standard BioTools Cat# 3089005B 1:200 Anti-I-A/I-E (MHCII) (clone M5/114.15.2 ) Biolegend Cat# 107637 1:800 Anti-CD4 (clone RMP4-5) Biolegend Cat# 100506 1:200 Anti-CD64 (clone X54-5/7.1) Biolegend Cat# 139302 1:50 Anti-Ly6G (clone RPA-T4) Biolegend Cat# 127620 1:200 Anti-Tbet (clone 4B10) Biolegend Cat# 644805 1:75 Anti-VISTA (clone MIH63) Biolegend Cat# 150202 1:200 Anti-TNFa (clone MP6-XT22) Standard BioTools Cat# 3162002B 1:50 Anti-PD-L2 (clone Ty25) Biolegend Cat# 107202 1:100 Anti-CD103 (clone 2E7) Biolegend Cat# 121402 1:200 Anti-IFNG (clone XMG1.2) Biolegend Cat# 505802 1:25 Anti-Eomes (clone Dan11mag) eBioscience Cat# 14-4875-82 1:50 Anti-CD80 (clone 16-10A1) Biolegend Cat# 104710 1:800 Anti-CD163 (clone EPR19518) Abcam Cat# ab182422 1:50 Anti-GATA3 (clone TWAJ) eBioscience Cat# 14-9966-82 1:100 Anti-ICOS (clone 7E.17G9) eBioscience Cat# 14-9942-85 1:100 Anti-F4/80 (clone BM8) Biolegend Cat# 123143 1:100 Anti-CD86 (clone GL-1) Biolegend Cat# 105002 1:100 Anti-GranzymeB (clone QA16A02) Biolegend Cat# 372202 1:100 Anti-CD115 (clone AFS98) Standard BioTools Cat# 3144012B 1:50 Anti-FoxP3 (clone FJK-16s) Standard BioTools Cat# 3158003A 1:100 Anti- CD8a (clone 53-6.7) Standard BioTools Cat# other:Article Title: Negative costimulation constrains T cell differentiation by imposing boundaries on possible cell states Article Snippet: anti-CD8a 146Nd , Article Title: PARP-inhibition reprograms macrophages toward an anti-tumor phenotype. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Olaparib MCE Cat#HY-10162 H2DCFDA (H2-DCF, DCF) ThermoFisher Cat#D399 N-Methylnicotinamide (MNA) FisherScientific Cat#M037425G Mitoquinone (MitoQ10) mesylate Selleck Cat#S8978 Rotenone Agilent Cat#103015-100 Trolox MCE Cat#HY-101445 MitoSOX ThermoFisher Cat#M36008 b-Estradiol Sigma-Aldrich Cat#E8875 Clodronate Liposomes & Control Liposomes (PBS) Liposoma Cat#CP-050-050 Recombinant murine IL-4 Peprotech Cat#214-14 Recombinant murine M-CSF Peprotech Cat#315-02 Recombinant mouse IL-10 Prospec Bio Cat#CYT-497 Recombinant mouse GM-CSF Peprotech Cat#315-03 IVISbrite D-Luciferin Potassium Salt Bioluminescent Substrate (1g) (XenoLight) PerkinElmer Cat#122799 Critical commercial assays QtrackerTM 625 Cell Labeling Kit ThermoFisher Cat#A10198 NAD/NADH Quantitation Colorimetric Kit Biovision Cat#K337 CellTraceTM Violet Cell Proliferation Kit ThermoFisher Cat#C34557 Double stain IHC kit Abcam Cat#ab183285 Opal 7-Color IHC Kit Akoya Cat# NEL811001KT Seahorse XF Glycolysis Stress Test Kit Seahorse Bioscience Cat#103020-100 Seahorse XF Cell Mito Stress Test Kit Seahorse Bioscience Cat#103015-100 Deposited data RNA seq data This paper GEO: GSE210378 Mass Spectrometry data This paper https://doi.org/10.6084/m9.figshare.20640090 https://doi.org/10.6084/m9.figshare.20639979 https://doi.org/10.6084/m9.figshare.20639895 https://doi.org/10.6084/m9.figshare.20640195 EMBL-EBI MetaboLights database https://doi.org/ 10.1093/nar/gkz1019, PMID:31691833 with the identifier MTBLS5928 Code for Akoya data analysis This paper https://github.com/WulfLab/MacrophagM- Quantification.git or https://doi.org/10.5281/ zenodo.7071901 Experimental models: Cell lines K14-GFP This paper N/A iBMPC This paper N/A Experimental models: Organisms/strains Mouse: FVB/129P2Ola This paper N/A Mouse: NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) Jackson Laboratory RRID: IMSR_JAX:005557 Mouse: CB17.Cg-PrkdcscidLystbg-J/Crl (Scid/Beige) Charles River RRID: IMSR_CRL:250 Mouse: 129S-Parp1tm1Zqw/J (Parp1 null) Jackson Laboratory RRID: IMSR_JAX:002779 Mouse: Parp2 knockout Peter Bai Lab N/A Mouse: C57BL6/J Jackson Laboratory RRID: IMSR_JAX:000664 Mouse: C57BL/6-Tg(TcraTcrb)1100Mjb/J (OTI) Jackson Laboratory RRID: IMSR_JAX:003831 Mouse: FVB/NJ (FVB) Jackson Laboratory RRID: IMSR_JAX:001800 Oligonucleotides qPCR primers for Figures 3 and S4, see Table S3 This paper N/A (Continued on next page) Cell Reports 41, 111462, October 11, 2022 e2 Article Title: Optimization and validation of CAR transduction into human primary NK cells using CRISPR and AAV Article Snippet: 146Nd_CD8a , |