Recombinant:Article Title: BTN2A1 acquisition through trogocytosis enhances Vγ9Vδ2 T cell cytotoxicity against autologous and cancer cells.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 158Gd-SIRPa (15–414) BioLegend Cat# 372102; RRID:AB_2629807 160Gd-ICT01 (20.1) ImCheck Ther. .. Cat# homemade 165Ho-CD40 (5C3) Standard BioTools Cat# 3165005B; RRID: AB_3716565 115In-CD163 (GHI/61) BioLegend Cat# 333602; RRID:AB_1088991 175Lu-PD-L1 (29E.2A3) Standard BioTools Cat# 3175017B; RRID:AB_2687638 143Nd-CD123 (6H6) Standard BioTools Cat# 3143014B; RRID:AB_2811081 144Nd-CD195-CCR5 (NP-6G4) Standard BioTools Cat# 3144007A; RRID:AB_2892770 145Nd-CD16 (3G8) Standard BioTools Cat# 3145008B; RRID:AB_2888924 146Nd-CD64 (10,1) Standard BioTools Cat# 3146006B; RRID: AB_3716566 148Nd-CD14 (RMO52) Standard BioTools Cat# 3148010B; RRID:AB_2858228 150Nd-CD86 (IT2.2) Standard BioTools Cat# 3150020B; RRID: AB_3716567 141Pr-CD68 (Y1/82A) BioLegend Cat# 333802; RRID:AB_1089058 147Sm-CD303 (201A) Standard BioTools Cat# 3147009B; RRID: AB_3716568 149Sm-CD141 (M80) BioLegend Cat# 344102; RRID:AB_2201808 152Sm-ICT03 (8.16) ImCheck Ther Cat# homemade 154Sm-IL6 (MQ2-13A5) Standard BioTools Cat# 3154011B; RRID:AB_3665167 159Tb-ICOSL (2D3) BioLegend Cat# 309402; RRID:AB_314768 169Tm-CD1d (51.1) BioLegend Cat# 350302; RRID:AB_10641848 89Y-CD45 (HI30) BioLegend Cat# 3089003B; RRID:AB_2938863 171Yb-ILT4 (42D1) BioLegend Cat# 338713; RRID:AB_2814237 172Yb-PD-L2 (24F.10C12) Standard BioTools Cat# 3172014B; RRID:AB_2877093 173Yb-HLADR (L243) Standard BioTools Cat# 3173005B; RRID:AB_2810248 174Yb-PVR (TX24) BioLegend Cat#337502; RRID:AB_1279096 176Yb-Nectin2 (TX31) BioLegend Cat# 337402; RRID:AB_2174164 Biological samples Human PBMC from healthy volunteers Etablissement Franç ais du Sang authorization number AC-2019-3428 Human PBMC from endometrial cancer patients GCBio protocol clinicalTrials.gov NCT01977274 Chemicals, peptides, and recombinant proteins Loxoribine Invitrogen Cat#tlrl-lox Recombinant human Interferon α 2b ImmunoTools Cat#11343516 Cyclosporin A Novartis CAS number 59865-13-3 rhBTN2A1-Fc R&D Biotech Cat#AVI10909-050 Proleukin Clinigen N/A Zoledronic acid Sigma-Aldrich CAS number 165800-06-6 Critical commercial assays Dynabeads TM Human T-Activator CD3/CD28 for T cell Expansion and Activation ThermoFisher RRID:AB_2916088 CD14 Microbeads, human kit Miltenyi Biotec Cat#130-050-201; RRID:AB_2665482 Quantum TM Simply Cellular® anti-Mouse IgG Kit Bangs Laboratories, Inc Cat#FCSC815B; RRID:AB_10064985 PKH67 Green Fluorescent Cell Linker Mini Kit for General Cell Membrane Labeling Sigma-Aldrich Cat#MINI67-1KT; RRID: AB_3716569 Caspase Glo 3/7 assay Promega Cat#G8090 Cell Proliferation Dye eFluor TM 670 Thermo fisher Scientific Cat#65-0840-85 CellEvent TM Caspase-3/7 Detection Reagents Invitrogen Cat#C10433 Experimental models: Cell lines ..
Activation Assay:Article Title: BTN2A1 acquisition through trogocytosis enhances Vγ9Vδ2 T cell cytotoxicity against autologous and cancer cells.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 158Gd-SIRPa (15–414) BioLegend Cat# 372102; RRID:AB_2629807 160Gd-ICT01 (20.1) ImCheck Ther. .. Cat# homemade 165Ho-CD40 (5C3) Standard BioTools Cat# 3165005B; RRID: AB_3716565 115In-CD163 (GHI/61) BioLegend Cat# 333602; RRID:AB_1088991 175Lu-PD-L1 (29E.2A3) Standard BioTools Cat# 3175017B; RRID:AB_2687638 143Nd-CD123 (6H6) Standard BioTools Cat# 3143014B; RRID:AB_2811081 144Nd-CD195-CCR5 (NP-6G4) Standard BioTools Cat# 3144007A; RRID:AB_2892770 145Nd-CD16 (3G8) Standard BioTools Cat# 3145008B; RRID:AB_2888924 146Nd-CD64 (10,1) Standard BioTools Cat# 3146006B; RRID: AB_3716566 148Nd-CD14 (RMO52) Standard BioTools Cat# 3148010B; RRID:AB_2858228 150Nd-CD86 (IT2.2) Standard BioTools Cat# 3150020B; RRID: AB_3716567 141Pr-CD68 (Y1/82A) BioLegend Cat# 333802; RRID:AB_1089058 147Sm-CD303 (201A) Standard BioTools Cat# 3147009B; RRID: AB_3716568 149Sm-CD141 (M80) BioLegend Cat# 344102; RRID:AB_2201808 152Sm-ICT03 (8.16) ImCheck Ther Cat# homemade 154Sm-IL6 (MQ2-13A5) Standard BioTools Cat# 3154011B; RRID:AB_3665167 159Tb-ICOSL (2D3) BioLegend Cat# 309402; RRID:AB_314768 169Tm-CD1d (51.1) BioLegend Cat# 350302; RRID:AB_10641848 89Y-CD45 (HI30) BioLegend Cat# 3089003B; RRID:AB_2938863 171Yb-ILT4 (42D1) BioLegend Cat# 338713; RRID:AB_2814237 172Yb-PD-L2 (24F.10C12) Standard BioTools Cat# 3172014B; RRID:AB_2877093 173Yb-HLADR (L243) Standard BioTools Cat# 3173005B; RRID:AB_2810248 174Yb-PVR (TX24) BioLegend Cat#337502; RRID:AB_1279096 176Yb-Nectin2 (TX31) BioLegend Cat# 337402; RRID:AB_2174164 Biological samples Human PBMC from healthy volunteers Etablissement Franç ais du Sang authorization number AC-2019-3428 Human PBMC from endometrial cancer patients GCBio protocol clinicalTrials.gov NCT01977274 Chemicals, peptides, and recombinant proteins Loxoribine Invitrogen Cat#tlrl-lox Recombinant human Interferon α 2b ImmunoTools Cat#11343516 Cyclosporin A Novartis CAS number 59865-13-3 rhBTN2A1-Fc R&D Biotech Cat#AVI10909-050 Proleukin Clinigen N/A Zoledronic acid Sigma-Aldrich CAS number 165800-06-6 Critical commercial assays Dynabeads TM Human T-Activator CD3/CD28 for T cell Expansion and Activation ThermoFisher RRID:AB_2916088 CD14 Microbeads, human kit Miltenyi Biotec Cat#130-050-201; RRID:AB_2665482 Quantum TM Simply Cellular® anti-Mouse IgG Kit Bangs Laboratories, Inc Cat#FCSC815B; RRID:AB_10064985 PKH67 Green Fluorescent Cell Linker Mini Kit for General Cell Membrane Labeling Sigma-Aldrich Cat#MINI67-1KT; RRID: AB_3716569 Caspase Glo 3/7 assay Promega Cat#G8090 Cell Proliferation Dye eFluor TM 670 Thermo fisher Scientific Cat#65-0840-85 CellEvent TM Caspase-3/7 Detection Reagents Invitrogen Cat#C10433 Experimental models: Cell lines ..
Membrane:Article Title: BTN2A1 acquisition through trogocytosis enhances Vγ9Vδ2 T cell cytotoxicity against autologous and cancer cells.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 158Gd-SIRPa (15–414) BioLegend Cat# 372102; RRID:AB_2629807 160Gd-ICT01 (20.1) ImCheck Ther. .. Cat# homemade 165Ho-CD40 (5C3) Standard BioTools Cat# 3165005B; RRID: AB_3716565 115In-CD163 (GHI/61) BioLegend Cat# 333602; RRID:AB_1088991 175Lu-PD-L1 (29E.2A3) Standard BioTools Cat# 3175017B; RRID:AB_2687638 143Nd-CD123 (6H6) Standard BioTools Cat# 3143014B; RRID:AB_2811081 144Nd-CD195-CCR5 (NP-6G4) Standard BioTools Cat# 3144007A; RRID:AB_2892770 145Nd-CD16 (3G8) Standard BioTools Cat# 3145008B; RRID:AB_2888924 146Nd-CD64 (10,1) Standard BioTools Cat# 3146006B; RRID: AB_3716566 148Nd-CD14 (RMO52) Standard BioTools Cat# 3148010B; RRID:AB_2858228 150Nd-CD86 (IT2.2) Standard BioTools Cat# 3150020B; RRID: AB_3716567 141Pr-CD68 (Y1/82A) BioLegend Cat# 333802; RRID:AB_1089058 147Sm-CD303 (201A) Standard BioTools Cat# 3147009B; RRID: AB_3716568 149Sm-CD141 (M80) BioLegend Cat# 344102; RRID:AB_2201808 152Sm-ICT03 (8.16) ImCheck Ther Cat# homemade 154Sm-IL6 (MQ2-13A5) Standard BioTools Cat# 3154011B; RRID:AB_3665167 159Tb-ICOSL (2D3) BioLegend Cat# 309402; RRID:AB_314768 169Tm-CD1d (51.1) BioLegend Cat# 350302; RRID:AB_10641848 89Y-CD45 (HI30) BioLegend Cat# 3089003B; RRID:AB_2938863 171Yb-ILT4 (42D1) BioLegend Cat# 338713; RRID:AB_2814237 172Yb-PD-L2 (24F.10C12) Standard BioTools Cat# 3172014B; RRID:AB_2877093 173Yb-HLADR (L243) Standard BioTools Cat# 3173005B; RRID:AB_2810248 174Yb-PVR (TX24) BioLegend Cat#337502; RRID:AB_1279096 176Yb-Nectin2 (TX31) BioLegend Cat# 337402; RRID:AB_2174164 Biological samples Human PBMC from healthy volunteers Etablissement Franç ais du Sang authorization number AC-2019-3428 Human PBMC from endometrial cancer patients GCBio protocol clinicalTrials.gov NCT01977274 Chemicals, peptides, and recombinant proteins Loxoribine Invitrogen Cat#tlrl-lox Recombinant human Interferon α 2b ImmunoTools Cat#11343516 Cyclosporin A Novartis CAS number 59865-13-3 rhBTN2A1-Fc R&D Biotech Cat#AVI10909-050 Proleukin Clinigen N/A Zoledronic acid Sigma-Aldrich CAS number 165800-06-6 Critical commercial assays Dynabeads TM Human T-Activator CD3/CD28 for T cell Expansion and Activation ThermoFisher RRID:AB_2916088 CD14 Microbeads, human kit Miltenyi Biotec Cat#130-050-201; RRID:AB_2665482 Quantum TM Simply Cellular® anti-Mouse IgG Kit Bangs Laboratories, Inc Cat#FCSC815B; RRID:AB_10064985 PKH67 Green Fluorescent Cell Linker Mini Kit for General Cell Membrane Labeling Sigma-Aldrich Cat#MINI67-1KT; RRID: AB_3716569 Caspase Glo 3/7 assay Promega Cat#G8090 Cell Proliferation Dye eFluor TM 670 Thermo fisher Scientific Cat#65-0840-85 CellEvent TM Caspase-3/7 Detection Reagents Invitrogen Cat#C10433 Experimental models: Cell lines ..
Labeling:Article Title: BTN2A1 acquisition through trogocytosis enhances Vγ9Vδ2 T cell cytotoxicity against autologous and cancer cells.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 158Gd-SIRPa (15–414) BioLegend Cat# 372102; RRID:AB_2629807 160Gd-ICT01 (20.1) ImCheck Ther. .. Cat# homemade 165Ho-CD40 (5C3) Standard BioTools Cat# 3165005B; RRID: AB_3716565 115In-CD163 (GHI/61) BioLegend Cat# 333602; RRID:AB_1088991 175Lu-PD-L1 (29E.2A3) Standard BioTools Cat# 3175017B; RRID:AB_2687638 143Nd-CD123 (6H6) Standard BioTools Cat# 3143014B; RRID:AB_2811081 144Nd-CD195-CCR5 (NP-6G4) Standard BioTools Cat# 3144007A; RRID:AB_2892770 145Nd-CD16 (3G8) Standard BioTools Cat# 3145008B; RRID:AB_2888924 146Nd-CD64 (10,1) Standard BioTools Cat# 3146006B; RRID: AB_3716566 148Nd-CD14 (RMO52) Standard BioTools Cat# 3148010B; RRID:AB_2858228 150Nd-CD86 (IT2.2) Standard BioTools Cat# 3150020B; RRID: AB_3716567 141Pr-CD68 (Y1/82A) BioLegend Cat# 333802; RRID:AB_1089058 147Sm-CD303 (201A) Standard BioTools Cat# 3147009B; RRID: AB_3716568 149Sm-CD141 (M80) BioLegend Cat# 344102; RRID:AB_2201808 152Sm-ICT03 (8.16) ImCheck Ther Cat# homemade 154Sm-IL6 (MQ2-13A5) Standard BioTools Cat# 3154011B; RRID:AB_3665167 159Tb-ICOSL (2D3) BioLegend Cat# 309402; RRID:AB_314768 169Tm-CD1d (51.1) BioLegend Cat# 350302; RRID:AB_10641848 89Y-CD45 (HI30) BioLegend Cat# 3089003B; RRID:AB_2938863 171Yb-ILT4 (42D1) BioLegend Cat# 338713; RRID:AB_2814237 172Yb-PD-L2 (24F.10C12) Standard BioTools Cat# 3172014B; RRID:AB_2877093 173Yb-HLADR (L243) Standard BioTools Cat# 3173005B; RRID:AB_2810248 174Yb-PVR (TX24) BioLegend Cat#337502; RRID:AB_1279096 176Yb-Nectin2 (TX31) BioLegend Cat# 337402; RRID:AB_2174164 Biological samples Human PBMC from healthy volunteers Etablissement Franç ais du Sang authorization number AC-2019-3428 Human PBMC from endometrial cancer patients GCBio protocol clinicalTrials.gov NCT01977274 Chemicals, peptides, and recombinant proteins Loxoribine Invitrogen Cat#tlrl-lox Recombinant human Interferon α 2b ImmunoTools Cat#11343516 Cyclosporin A Novartis CAS number 59865-13-3 rhBTN2A1-Fc R&D Biotech Cat#AVI10909-050 Proleukin Clinigen N/A Zoledronic acid Sigma-Aldrich CAS number 165800-06-6 Critical commercial assays Dynabeads TM Human T-Activator CD3/CD28 for T cell Expansion and Activation ThermoFisher RRID:AB_2916088 CD14 Microbeads, human kit Miltenyi Biotec Cat#130-050-201; RRID:AB_2665482 Quantum TM Simply Cellular® anti-Mouse IgG Kit Bangs Laboratories, Inc Cat#FCSC815B; RRID:AB_10064985 PKH67 Green Fluorescent Cell Linker Mini Kit for General Cell Membrane Labeling Sigma-Aldrich Cat#MINI67-1KT; RRID: AB_3716569 Caspase Glo 3/7 assay Promega Cat#G8090 Cell Proliferation Dye eFluor TM 670 Thermo fisher Scientific Cat#65-0840-85 CellEvent TM Caspase-3/7 Detection Reagents Invitrogen Cat#C10433 Experimental models: Cell lines ..
Caspase-Glo Assay:Article Title: BTN2A1 acquisition through trogocytosis enhances Vγ9Vδ2 T cell cytotoxicity against autologous and cancer cells.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 158Gd-SIRPa (15–414) BioLegend Cat# 372102; RRID:AB_2629807 160Gd-ICT01 (20.1) ImCheck Ther. .. Cat# homemade 165Ho-CD40 (5C3) Standard BioTools Cat# 3165005B; RRID: AB_3716565 115In-CD163 (GHI/61) BioLegend Cat# 333602; RRID:AB_1088991 175Lu-PD-L1 (29E.2A3) Standard BioTools Cat# 3175017B; RRID:AB_2687638 143Nd-CD123 (6H6) Standard BioTools Cat# 3143014B; RRID:AB_2811081 144Nd-CD195-CCR5 (NP-6G4) Standard BioTools Cat# 3144007A; RRID:AB_2892770 145Nd-CD16 (3G8) Standard BioTools Cat# 3145008B; RRID:AB_2888924 146Nd-CD64 (10,1) Standard BioTools Cat# 3146006B; RRID: AB_3716566 148Nd-CD14 (RMO52) Standard BioTools Cat# 3148010B; RRID:AB_2858228 150Nd-CD86 (IT2.2) Standard BioTools Cat# 3150020B; RRID: AB_3716567 141Pr-CD68 (Y1/82A) BioLegend Cat# 333802; RRID:AB_1089058 147Sm-CD303 (201A) Standard BioTools Cat# 3147009B; RRID: AB_3716568 149Sm-CD141 (M80) BioLegend Cat# 344102; RRID:AB_2201808 152Sm-ICT03 (8.16) ImCheck Ther Cat# homemade 154Sm-IL6 (MQ2-13A5) Standard BioTools Cat# 3154011B; RRID:AB_3665167 159Tb-ICOSL (2D3) BioLegend Cat# 309402; RRID:AB_314768 169Tm-CD1d (51.1) BioLegend Cat# 350302; RRID:AB_10641848 89Y-CD45 (HI30) BioLegend Cat# 3089003B; RRID:AB_2938863 171Yb-ILT4 (42D1) BioLegend Cat# 338713; RRID:AB_2814237 172Yb-PD-L2 (24F.10C12) Standard BioTools Cat# 3172014B; RRID:AB_2877093 173Yb-HLADR (L243) Standard BioTools Cat# 3173005B; RRID:AB_2810248 174Yb-PVR (TX24) BioLegend Cat#337502; RRID:AB_1279096 176Yb-Nectin2 (TX31) BioLegend Cat# 337402; RRID:AB_2174164 Biological samples Human PBMC from healthy volunteers Etablissement Franç ais du Sang authorization number AC-2019-3428 Human PBMC from endometrial cancer patients GCBio protocol clinicalTrials.gov NCT01977274 Chemicals, peptides, and recombinant proteins Loxoribine Invitrogen Cat#tlrl-lox Recombinant human Interferon α 2b ImmunoTools Cat#11343516 Cyclosporin A Novartis CAS number 59865-13-3 rhBTN2A1-Fc R&D Biotech Cat#AVI10909-050 Proleukin Clinigen N/A Zoledronic acid Sigma-Aldrich CAS number 165800-06-6 Critical commercial assays Dynabeads TM Human T-Activator CD3/CD28 for T cell Expansion and Activation ThermoFisher RRID:AB_2916088 CD14 Microbeads, human kit Miltenyi Biotec Cat#130-050-201; RRID:AB_2665482 Quantum TM Simply Cellular® anti-Mouse IgG Kit Bangs Laboratories, Inc Cat#FCSC815B; RRID:AB_10064985 PKH67 Green Fluorescent Cell Linker Mini Kit for General Cell Membrane Labeling Sigma-Aldrich Cat#MINI67-1KT; RRID: AB_3716569 Caspase Glo 3/7 assay Promega Cat#G8090 Cell Proliferation Dye eFluor TM 670 Thermo fisher Scientific Cat#65-0840-85 CellEvent TM Caspase-3/7 Detection Reagents Invitrogen Cat#C10433 Experimental models: Cell lines ..
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