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human cardiac fibroblasts: hcf  (Cell Applications Inc)


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    Structured Review

    Cell Applications Inc human cardiac fibroblasts: hcf
    Human Cardiac Fibroblasts: Hcf, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 95/100, based on 116 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/05a/Human+Cardiac+Fibroblasts%3A+HCF/custom%402148-01%4042715682
    Average 95 stars, based on 116 article reviews
    human cardiac fibroblasts: hcf - by Bioz Stars, 2026-09
    95/100 stars

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    other:

    Article Title: CAR-T cells targeting HLA-G as potent therapeutic strategy for EGFR-mutated and overexpressed oral cancer
    Article Snippet: Human corneal epithelial cells , Cell Applications , Cat# 630-05a, RRID: N/A.

    Article Title: Glucocorticoids regulate the human non-coding genome
    Article Snippet: 0000419228, 0000650386, 0000657512, and 0000583849) were cultured in SkGM-2 medium (Lonza; cat. no. CC-3245).

    Derivative Assay:

    Article Title: Anti-Inflammatory Effect of Atorvastatin on Primary Human Endothelial Cells Incubated under Genotoxic Load.
    Article Snippet: The level of cytokine expression was measured in human coronary artery (HCAEC) and internal thoracic artery (HITAEC) endothelial cells exposed to 500 ng/ml alkylating mutagen mitomycin C (MMC) and 5 μM atorvastatin.. It was found that treatment of MMC-exposed HCAEC with atorvastatin decreased secretion of macrophage migration inhibitory factor (MIF), IL-8, and IL8 gene expression, but increased the expression of SERPINE1 gene encoding the PAI-1 protein.. In atorvastatin-treated HITAEC, the concentration of MIF protein and the expression of the IL8 and SERPINE1 genes were reduced.

    Cell Culture:

    Article Title: Synthetic Peptide LKEKK as Potential Antipsoriatic Drag
    Article Snippet: .. Normal human epidermal keratinocytes (Cryopreserved HEK, Adult: Frozen HEK (5x10^5), CAT.# 102-05a) were obtained from Cell Applications, Inc. (USA) and were cultured for 24 h in Keratinocyte serum-free medium EpiGro containing EpiLife undefined growth supplement (Thermo Fisher Scientific, USA) in a 5% CO2 incubator at 37°C and were used at the second or third passage. ..

    Modification:

    Article Title: Structure and function of modular microfluidic device for in-vitro modeling of human organs
    Article Snippet: Poly-L-lysine (PLL) solution, 0.1% (w/v), collagen (type 1, rat tail) and Trypsin/EDTA were purchased from Sigma Aldrich (Burlington, Massachusetts, USA). .. Dulbecco's Modified Eagle Medium/Ham's F-12 (DMEM/F12) containing 10% (v/v) fetal bovine serum and 1% penicillin-streptomycin antibiotic was purchased from Gibco., human pre-adipocytes (HPAd) cat #802s-05a, pre-adipocyte growth medium, adipocyte differentiation medium, adipocyte maintenance medium, and adipocyte starvation medium from Cell Applications (USA); iMDM medium from Thermo Fisher Scientific (USA); Lipid A (LPA) from E. coli serotype R515 (TLR grade) from Alexis Biochemical (SA, USA); TNF-α, IL-6 and IL-8 ELISA kits, CD11b-FITC (clone ICRF44), CD45-PE (clone: 2D1), CD3-PerCP-Cy5.5 (cline: HIT3A), CD19-PE-Cy7 (clone: calceine-AM from ThermoFisher Scientific (USA); 2-NBDG glucose uptake cell-based assay kit from Cayman Chemical (USA); Hoechst 33342 from Molecular Probe (OR, USA); Bradford reagent and 0.4% trypan blue, metformin hydrochloride and docosapentaenoic acid (DPA) from Sigma Aldrich (USA); Other chemicals were of analytical grades from Sigma Aldrich (USA). .. Fabrication: The microfluidic systems can be fabricated using a variety of micro fabrication techniques including silicon micromachining, soft lithography, injection molding, 3D printing and laser machining.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Structure and function of modular microfluidic device for in-vitro modeling of human organs
    Article Snippet: Poly-L-lysine (PLL) solution, 0.1% (w/v), collagen (type 1, rat tail) and Trypsin/EDTA were purchased from Sigma Aldrich (Burlington, Massachusetts, USA). .. Dulbecco's Modified Eagle Medium/Ham's F-12 (DMEM/F12) containing 10% (v/v) fetal bovine serum and 1% penicillin-streptomycin antibiotic was purchased from Gibco., human pre-adipocytes (HPAd) cat #802s-05a, pre-adipocyte growth medium, adipocyte differentiation medium, adipocyte maintenance medium, and adipocyte starvation medium from Cell Applications (USA); iMDM medium from Thermo Fisher Scientific (USA); Lipid A (LPA) from E. coli serotype R515 (TLR grade) from Alexis Biochemical (SA, USA); TNF-α, IL-6 and IL-8 ELISA kits, CD11b-FITC (clone ICRF44), CD45-PE (clone: 2D1), CD3-PerCP-Cy5.5 (cline: HIT3A), CD19-PE-Cy7 (clone: calceine-AM from ThermoFisher Scientific (USA); 2-NBDG glucose uptake cell-based assay kit from Cayman Chemical (USA); Hoechst 33342 from Molecular Probe (OR, USA); Bradford reagent and 0.4% trypan blue, metformin hydrochloride and docosapentaenoic acid (DPA) from Sigma Aldrich (USA); Other chemicals were of analytical grades from Sigma Aldrich (USA). .. Fabrication: The microfluidic systems can be fabricated using a variety of micro fabrication techniques including silicon micromachining, soft lithography, injection molding, 3D printing and laser machining.

    Cell Based Assay:

    Article Title: Structure and function of modular microfluidic device for in-vitro modeling of human organs
    Article Snippet: Poly-L-lysine (PLL) solution, 0.1% (w/v), collagen (type 1, rat tail) and Trypsin/EDTA were purchased from Sigma Aldrich (Burlington, Massachusetts, USA). .. Dulbecco's Modified Eagle Medium/Ham's F-12 (DMEM/F12) containing 10% (v/v) fetal bovine serum and 1% penicillin-streptomycin antibiotic was purchased from Gibco., human pre-adipocytes (HPAd) cat #802s-05a, pre-adipocyte growth medium, adipocyte differentiation medium, adipocyte maintenance medium, and adipocyte starvation medium from Cell Applications (USA); iMDM medium from Thermo Fisher Scientific (USA); Lipid A (LPA) from E. coli serotype R515 (TLR grade) from Alexis Biochemical (SA, USA); TNF-α, IL-6 and IL-8 ELISA kits, CD11b-FITC (clone ICRF44), CD45-PE (clone: 2D1), CD3-PerCP-Cy5.5 (cline: HIT3A), CD19-PE-Cy7 (clone: calceine-AM from ThermoFisher Scientific (USA); 2-NBDG glucose uptake cell-based assay kit from Cayman Chemical (USA); Hoechst 33342 from Molecular Probe (OR, USA); Bradford reagent and 0.4% trypan blue, metformin hydrochloride and docosapentaenoic acid (DPA) from Sigma Aldrich (USA); Other chemicals were of analytical grades from Sigma Aldrich (USA). .. Fabrication: The microfluidic systems can be fabricated using a variety of micro fabrication techniques including silicon micromachining, soft lithography, injection molding, 3D printing and laser machining.



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