Journal: International Journal of Molecular Sciences

Article Title: Pilot Proteomic Analysis of Urinary Extracellular Vesicles Supports the “Toxic Urine Hypothesis” as a Vicious Cycle in Refractory IC/BPS Pathogenesis

doi: 10.3390/ijms27010130

Figure Lengend Snippet: Urinary extracellular vesicles from IC/BPS patients enhance NF-κB activity in HEK293T reporter cells and human bladder epithelial cells. ( A ) Schematic representation of the dual-luciferase reporter assay workflow. HEK293T cells were seeded at 0 h, transfected with NF-κB reporter (Firefly luciferase) and internal control (Renilla luciferase) plasmids at 16 h, stimulated with uEVs (50 µg/mL) or TNFα (10 ng/mL) at 32 h, and assessed for luciferase activity at 56 h. pGL3-Basic vector lacking NF-κB binding sites served as a negative control. ( B ) NF-κB activity represented as normalized Firefly/Renilla luciferase ratios. Data are presented as the mean ± SEM. Statistical significance was determined between the indicated groups: *, p < 0.05. ( C ) Here, 50 µg/mL of uEVs from a healthy donor (H) or an IC patient (Pt) was used to treat human bladder epithelial cells (HBlEpC) for 48 h. The protein expressions of 14-3-3ζ, p-NF-κB p65 at serine536, and total NF-κB p65 were determined via Western blot. GAPDH was used as an internal protein loading control. The numbers below the blots indicate expression levels relative to H uEV–treated cells after normalization to GAPDH.

Article Snippet: For the detection of cellular proteins, human bladder epithelial cells (HBlEpC; Cell Applications, Inc., San Diego, CA, USA) were treated with uEVs for 48 h and lysed with RIPA buffer, followed by SDS-PAGE and immunoblot analysis using the indicated antibodies.

Techniques: Activity Assay, Luciferase, Reporter Assay, Transfection, Control, Plasmid Preparation, Binding Assay, Negative Control, Western Blot, Expressing

Journal: Advanced Healthcare Materials

Article Title: Plasma‐Polymerized Nanoparticles Presenting Fibrillin‐1 Drive Rapid Re‐Endothelialization of Vascular Grafts

doi: 10.1002/adhm.202503360

Figure Lengend Snippet: PPN‐PF8 functionalization of ePTFE improves endothelial attachment in vitro. A) Representative images of SEM and F‐actin staining. B) HCAEC attachment and C) spreading on ePTFE, ePTFE with passively bound PF8 (ePTFE‐PF8), PPN‐coated ePTFE (PPN), and PPN‐PF8 functionalized ePTFE, n = 3 per sample. Scale bar = 300 µm. * p < 0.05, *** p < 0.001 versus ePTFE.

Article Snippet: Human coronary artery endothelial cells (HCAEC; Cell Applications) were cultured in MesoEndo medium (Cell Applications) at 37 °C in 5% CO 2 .

Techniques: In Vitro, Staining

Journal: Advanced Healthcare Materials

Article Title: Plasma‐Polymerized Nanoparticles Presenting Fibrillin‐1 Drive Rapid Re‐Endothelialization of Vascular Grafts

doi: 10.1002/adhm.202503360

Figure Lengend Snippet: PPN‐PF8 functionalization of ePTFE improves endothelial proliferation in vitro. A) Representative images of SEM and F‐actin staining of HCAEC proliferation on days 1 and 3. B) HCAEC proliferation and C) spreading on ePTFE, ePTFE with passively bound PF8, PPN, and PPN‐PF8 functionalized ePTFE. * p < 0.05, *** p < 0.001 versus ePTFE on each day, Scale bar = 300 µm. n = 3 per sample.

Article Snippet: Human coronary artery endothelial cells (HCAEC; Cell Applications) were cultured in MesoEndo medium (Cell Applications) at 37 °C in 5% CO 2 .

Techniques: In Vitro, Staining

Journal: Polymers

Article Title: Bacterial Inhibition and Osteogenic Potentials of Sr/Zn Co-Doped Nano-Hydroxyapatite-PLGA Composite Scaffold for Bone Tissue Engineering Applications

doi: 10.3390/polym15061370

Figure Lengend Snippet: MTT assay for osteoblasts cultured for 2 and 7 days on different scaffolds: (nHAp, nHAp-PLGA, 1% Zn/Sr-nHAp-PLGA, 2.5% Zn/Sr-nHAp-PLGA, and 4% Zn/Sr-nHAp-PLGA). *** p ≤ 0.001 compared to nHAp.

Article Snippet: In this study, primary rat osteoblasts’ cell line (ROb) was purchased from (Cell Applications Inc., San Diego, CA, USA) and all the tissue culture reagents were purchased from Sigma-Aldrich.

Techniques: MTT Assay, Cell Culture