human brain endothelial cells hbec5i  (ATCC)

Journal: Bioengineering

Article Title: A Versatile Microfluidic Device System that Lacks a Synthetic Extracellular Matrix Recapitulates the Blood–Brain Barrier and Dynamic Tumor Cell Interaction

doi: 10.3390/bioengineering11101008

Figure Lengend Snippet: The growth kinetics of the HBEC5i (endothelial cells), U87MG (glioblastoma tumoral cells), and astrocyte cell lines. The top central graphs show the quantification of the growth rate with representative micrographs of the time course of proliferation in the culture plate below each graph. The scale bar represents 100 µm in all.

Article Snippet: Human U87MG cells; glioblastoma multiforme from the American Type Culture Collection (ATCC, Manassas, VA, USA), with EMEM +10% fetal bovine serum [ ], Merck/Millipore Sigma Burlington, MA, USA), HBEC5i (from the ATCC, with DMEM/F12 +10% fetal bovine serum, made in Manassas, Virginia USA), and astrocytes from ScienCell, Carlsbad, CA, USA (catalog number 1800, with supplement culture medium 1801) were utilized, following the specifications described in the technical datasheet, All cell lines were maintained at 80% confluence for cell subculturing, and all experiments were conducted with subcultures of fewer than 8 subcultures. shows the general process carried out in this study.

Techniques:

Journal: Bioengineering

Article Title: A Versatile Microfluidic Device System that Lacks a Synthetic Extracellular Matrix Recapitulates the Blood–Brain Barrier and Dynamic Tumor Cell Interaction

doi: 10.3390/bioengineering11101008

Figure Lengend Snippet: The cell migration ability of ( A ) U87MG endothelial cells and ( B ) HBEC5i at different starting cell densities. Migrating cells are indicated in yellow.

Article Snippet: Human U87MG cells; glioblastoma multiforme from the American Type Culture Collection (ATCC, Manassas, VA, USA), with EMEM +10% fetal bovine serum [ ], Merck/Millipore Sigma Burlington, MA, USA), HBEC5i (from the ATCC, with DMEM/F12 +10% fetal bovine serum, made in Manassas, Virginia USA), and astrocytes from ScienCell, Carlsbad, CA, USA (catalog number 1800, with supplement culture medium 1801) were utilized, following the specifications described in the technical datasheet, All cell lines were maintained at 80% confluence for cell subculturing, and all experiments were conducted with subcultures of fewer than 8 subcultures. shows the general process carried out in this study.

Techniques: Migration

Journal: Bioengineering

Article Title: A Versatile Microfluidic Device System that Lacks a Synthetic Extracellular Matrix Recapitulates the Blood–Brain Barrier and Dynamic Tumor Cell Interaction

doi: 10.3390/bioengineering11101008

Figure Lengend Snippet: Co-culture of U87MG-HBEC5i acquired by confocal microscopy: the cell migration process. The cellular cytoskeleton is shown with the expression of phalloidin (green), and the nuclei were assessed with DAPI (blue). Cell–cell interactions are shown with yellow arrows, which occurred through interconnecting channels. The scale bar represents 100 µm.

Article Snippet: Human U87MG cells; glioblastoma multiforme from the American Type Culture Collection (ATCC, Manassas, VA, USA), with EMEM +10% fetal bovine serum [ ], Merck/Millipore Sigma Burlington, MA, USA), HBEC5i (from the ATCC, with DMEM/F12 +10% fetal bovine serum, made in Manassas, Virginia USA), and astrocytes from ScienCell, Carlsbad, CA, USA (catalog number 1800, with supplement culture medium 1801) were utilized, following the specifications described in the technical datasheet, All cell lines were maintained at 80% confluence for cell subculturing, and all experiments were conducted with subcultures of fewer than 8 subcultures. shows the general process carried out in this study.

Techniques: Co-Culture Assay, Confocal Microscopy, Migration, Expressing

Journal: Bioengineering

Article Title: A Versatile Microfluidic Device System that Lacks a Synthetic Extracellular Matrix Recapitulates the Blood–Brain Barrier and Dynamic Tumor Cell Interaction

doi: 10.3390/bioengineering11101008

Figure Lengend Snippet: The culture evolution at different time points and the process of cell invasion. Glioblastoma U87MG cells are shown in green, endothelial HBEC5i cells are shown in red, cell nuclei are shown in blue (DAPI), and cell–cell interactions through interconnecting channels are shown by green and red arrows. The expression of cytokines was carried out by combining the triplicate model to obtain the necessary amount of culture medium (cytokine sample = 1). The scale bar represents 200 µm.

Article Snippet: Human U87MG cells; glioblastoma multiforme from the American Type Culture Collection (ATCC, Manassas, VA, USA), with EMEM +10% fetal bovine serum [ ], Merck/Millipore Sigma Burlington, MA, USA), HBEC5i (from the ATCC, with DMEM/F12 +10% fetal bovine serum, made in Manassas, Virginia USA), and astrocytes from ScienCell, Carlsbad, CA, USA (catalog number 1800, with supplement culture medium 1801) were utilized, following the specifications described in the technical datasheet, All cell lines were maintained at 80% confluence for cell subculturing, and all experiments were conducted with subcultures of fewer than 8 subcultures. shows the general process carried out in this study.

Techniques: Expressing