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ythdf2 antibody  (Proteintech)


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    Structured Review

    Proteintech ythdf2 antibody
    Fig. 4 | <t>YTHDF2-null</t> muscles favor catabolic signaling. a Western blot analyses and quantification using total protein detection by Ponceau stain as a loading control for b phosphor-(p-)AKT, c AKT, d p-AKT/AKT ratio, e p-mTOR, f mTOR, g p-mTOR/AKT ratio, h LC3I, i LC3II, j LC3II/LC3I ratio, k p62, l Beclin-1, m monoubiquitin, and n polyubiquitin in Ctrl and Y2-KO quadriceps at 8 months of age. o Proteasomal activity as determined via colorimetric proteasome activity
    Ythdf2 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 480 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ythdf2/YTHDF2+Antibody/pm38467649-273-12-14
    Average 96 stars, based on 480 article reviews
    ythdf2 antibody - by Bioz Stars, 2026-11
    96/100 stars

    Images

    1) Product Images from "YTHDF2 governs muscle size through a targeted modulation of proteostasis."

    Article Title: YTHDF2 governs muscle size through a targeted modulation of proteostasis.

    Journal: Nature communications

    doi: 10.1038/s41467-024-46546-8

    Fig. 4 | YTHDF2-null muscles favor catabolic signaling. a Western blot analyses and quantification using total protein detection by Ponceau stain as a loading control for b phosphor-(p-)AKT, c AKT, d p-AKT/AKT ratio, e p-mTOR, f mTOR, g p-mTOR/AKT ratio, h LC3I, i LC3II, j LC3II/LC3I ratio, k p62, l Beclin-1, m monoubiquitin, and n polyubiquitin in Ctrl and Y2-KO quadriceps at 8 months of age. o Proteasomal activity as determined via colorimetric proteasome activity
    Figure Legend Snippet: Fig. 4 | YTHDF2-null muscles favor catabolic signaling. a Western blot analyses and quantification using total protein detection by Ponceau stain as a loading control for b phosphor-(p-)AKT, c AKT, d p-AKT/AKT ratio, e p-mTOR, f mTOR, g p-mTOR/AKT ratio, h LC3I, i LC3II, j LC3II/LC3I ratio, k p62, l Beclin-1, m monoubiquitin, and n polyubiquitin in Ctrl and Y2-KO quadriceps at 8 months of age. o Proteasomal activity as determined via colorimetric proteasome activity

    Techniques Used: Muscles, Western Blot, Staining, Control, Activity Assay

    Related Articles

    Western Blot:

    Article Title: GAS5 Downregulation Promotes the Progression of Triple-Negative Breast Cancer by Regulating Tumor Cells-MDSCs Crosstalk.
    Article Snippet: .. One-third of the precipitated material was eluted in 1× SDS loading buffer for Western blot analysis of YTHDF2 (Proteintech, Wuhan, China). ..

    Analysis:

    Article Title: GAS5 Downregulation Promotes the Progression of Triple-Negative Breast Cancer by Regulating Tumor Cells-MDSCs Crosstalk.
    Article Snippet: .. One-third of the precipitated material was eluted in 1× SDS loading buffer for Western blot analysis of YTHDF2 (Proteintech, Wuhan, China). ..

    Incubation:

    Article Title: HucMSC-sEV-derived miR-320d alleviates IBD by inhibiting macrophage ferroptosis through m6A-mediated BCAT2 upregulation.
    Article Snippet: N6-methyladenosine(m6A) modification has garnered significant attention due to its dynamic and reversible post-transcriptional regulation, playing a crucial role in the pathogenesis and progression of inflammatory bowel disease (IBD).. Previous studies have demonstrated that human umbilical cord mesenchymal stem cell-derived exosomes (hucMSC-sEV) alleviate IBD severity by dampening excessive immune responses and fostering a balanced immune environment.. Although hucMSC-sEV has been shown to alleviate IBD, whether its effects involve m6A modification remains unclear.

    Article Title: Helicobacter pylori CagA effector protein drives gastric cancer progression via m6A/DUSP6-mediated regulation of tumor glycolysis
    Article Snippet: Proteins were separated by 15% SDS-PAGE and subsequently transferred onto nitrocellulose membranes (Pall Corporation, USA). .. The membranes were blocked with 5% skim milk in Tris-buffered saline with Tween 20 (TBST) for 2 hours at room temperature, followed by overnight incubation at 4 °C with specific primary antibodies: METTL3 (15073- 1-AP, Proteintech, Wuhan, China, 1:1000), USP7 (66514-1-Ig, Proteintech, 1:1000), YTHDF2 (24744-1-AP, Proteintech, 1:3000), DUSP6 (R381008, Zenbio, Chengdu, China, 1:500), GAPDH (10494-1-AP, Proteintech, 1:5000), METTL14 (A8530, ABclonal, Wuhan, China, 1:1000), WTAP (10200-1-AP, Proteintech, 1:1000), YTHDF1 (19834-1-AP, Proteintech, 1:3000), and GFP-tag (50430-2-AP, Proteintech, 1:2000). .. After three washes with TBST, the membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies (Proteintech, 1:5000) for 1 hour at room temperature.

    Saline:

    Article Title: Helicobacter pylori CagA effector protein drives gastric cancer progression via m6A/DUSP6-mediated regulation of tumor glycolysis
    Article Snippet: Proteins were separated by 15% SDS-PAGE and subsequently transferred onto nitrocellulose membranes (Pall Corporation, USA). .. The membranes were blocked with 5% skim milk in Tris-buffered saline with Tween 20 (TBST) for 2 hours at room temperature, followed by overnight incubation at 4 °C with specific primary antibodies: METTL3 (15073- 1-AP, Proteintech, Wuhan, China, 1:1000), USP7 (66514-1-Ig, Proteintech, 1:1000), YTHDF2 (24744-1-AP, Proteintech, 1:3000), DUSP6 (R381008, Zenbio, Chengdu, China, 1:500), GAPDH (10494-1-AP, Proteintech, 1:5000), METTL14 (A8530, ABclonal, Wuhan, China, 1:1000), WTAP (10200-1-AP, Proteintech, 1:1000), YTHDF1 (19834-1-AP, Proteintech, 1:3000), and GFP-tag (50430-2-AP, Proteintech, 1:2000). .. After three washes with TBST, the membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies (Proteintech, 1:5000) for 1 hour at room temperature.



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    Image Search Results


    List of antibodies used for this study

    Journal: Experimental Hematology & Oncology

    Article Title: Tumor-intrinsic NSUN2 orchestrates immunosuppression in lung adenocarcinoma via the m 5 C-HDAC8-CCL5 axis

    doi: 10.1186/s40164-026-00826-9

    Figure Lengend Snippet: List of antibodies used for this study

    Article Snippet: A25318 , Anti-YTHDF2 , ABclonal.

    Techniques: Control

    The differentiated expression of YTHDF1 and YTHDF2 in tumor tissues and normal tissues. (A) YTHDF1 expression in non-paired lung cancer tissues and normal tissues according to the TCGA dataset. (B) YTHDF1 expression in paired lung cancer tissues and normal tissues in the TCGA database. (C) YTHDF2 expression in non-paired lung cancer tissues and normal tissues according to the TCGA dataset. (D) YTHDF2 expression in paired lung cancer tissues and normal tissues in the TCGA database. ns, not significant; *, P<0.05; ***, P<0.001. TCGA, The Cancer Genome Atlas.

    Journal: Journal of Thoracic Disease

    Article Title: Expression of YTHDF1 and YTHDF2 in lung squamous cell carcinoma and their correlation with PD-L1

    doi: 10.21037/jtd-2026-1-0312

    Figure Lengend Snippet: The differentiated expression of YTHDF1 and YTHDF2 in tumor tissues and normal tissues. (A) YTHDF1 expression in non-paired lung cancer tissues and normal tissues according to the TCGA dataset. (B) YTHDF1 expression in paired lung cancer tissues and normal tissues in the TCGA database. (C) YTHDF2 expression in non-paired lung cancer tissues and normal tissues according to the TCGA dataset. (D) YTHDF2 expression in paired lung cancer tissues and normal tissues in the TCGA database. ns, not significant; *, P<0.05; ***, P<0.001. TCGA, The Cancer Genome Atlas.

    Article Snippet: YTHDF2 antibodies were purchased from ABclonal Biotechnology Co., Ltd. (Wuhan, China), and Ethylene Glycol-bis (2-aminoethyl Ether)-N,N,N',N'-tetraacetic Acid (EGTA) antigen retrieval buffer (pH 9.0), antibody diluent, polylysine, phosphate buffered saline (PBS), super-sensitive 3,3'-diaminobenzidine (DAB) substrate, instant immunohistochemistry MaxVision TM kit, and neutral balsam were purchased from Fuzhou Maxim Biotechnology Co., Ltd. (Fuzhou, China).

    Techniques: Expressing

    Relationship between YTHDF1/YTHDF2 expression and prognosis of LUSC patients. (A,C) Association between YTHDF1, YTHDF2 expression and DSS of LUSC patients. (B,D) Association between YTHDF1, YTHDF2 expression and OS of LUSC patients. DSS, disease-specific survival; LUSC, lung squamous cell carcinoma; OS, overall survival.

    Journal: Journal of Thoracic Disease

    Article Title: Expression of YTHDF1 and YTHDF2 in lung squamous cell carcinoma and their correlation with PD-L1

    doi: 10.21037/jtd-2026-1-0312

    Figure Lengend Snippet: Relationship between YTHDF1/YTHDF2 expression and prognosis of LUSC patients. (A,C) Association between YTHDF1, YTHDF2 expression and DSS of LUSC patients. (B,D) Association between YTHDF1, YTHDF2 expression and OS of LUSC patients. DSS, disease-specific survival; LUSC, lung squamous cell carcinoma; OS, overall survival.

    Article Snippet: YTHDF2 antibodies were purchased from ABclonal Biotechnology Co., Ltd. (Wuhan, China), and Ethylene Glycol-bis (2-aminoethyl Ether)-N,N,N',N'-tetraacetic Acid (EGTA) antigen retrieval buffer (pH 9.0), antibody diluent, polylysine, phosphate buffered saline (PBS), super-sensitive 3,3'-diaminobenzidine (DAB) substrate, instant immunohistochemistry MaxVision TM kit, and neutral balsam were purchased from Fuzhou Maxim Biotechnology Co., Ltd. (Fuzhou, China).

    Techniques: Expressing

    Potential molecular mechanisms of YTHDF1 and YTHDF2 in LUSC progression. (A,C) Gene set enrichment analysis showed that YTHDF1 (A) and YTHDF2 (C) high expression subgroups of LUSC patients were significantly associated with E2F targets, G2M checkpoint, mitotic spindle, pancreas beta cells, spermatogenesis and other pathways. (B,D) Gene set enrichment analysis showed that YTHDF1 (B) and YTHDF2 (D) low expression subgroups of LUSC patients were significantly associated with allograft rejection, coagulation, inflammatory response, interferon gamma response and other pathways. LUSC, lung squamous cell carcinoma.

    Journal: Journal of Thoracic Disease

    Article Title: Expression of YTHDF1 and YTHDF2 in lung squamous cell carcinoma and their correlation with PD-L1

    doi: 10.21037/jtd-2026-1-0312

    Figure Lengend Snippet: Potential molecular mechanisms of YTHDF1 and YTHDF2 in LUSC progression. (A,C) Gene set enrichment analysis showed that YTHDF1 (A) and YTHDF2 (C) high expression subgroups of LUSC patients were significantly associated with E2F targets, G2M checkpoint, mitotic spindle, pancreas beta cells, spermatogenesis and other pathways. (B,D) Gene set enrichment analysis showed that YTHDF1 (B) and YTHDF2 (D) low expression subgroups of LUSC patients were significantly associated with allograft rejection, coagulation, inflammatory response, interferon gamma response and other pathways. LUSC, lung squamous cell carcinoma.

    Article Snippet: YTHDF2 antibodies were purchased from ABclonal Biotechnology Co., Ltd. (Wuhan, China), and Ethylene Glycol-bis (2-aminoethyl Ether)-N,N,N',N'-tetraacetic Acid (EGTA) antigen retrieval buffer (pH 9.0), antibody diluent, polylysine, phosphate buffered saline (PBS), super-sensitive 3,3'-diaminobenzidine (DAB) substrate, instant immunohistochemistry MaxVision TM kit, and neutral balsam were purchased from Fuzhou Maxim Biotechnology Co., Ltd. (Fuzhou, China).

    Techniques: Expressing, Coagulation

    Correlation of YTHDF2 expression levels with LUSC immune cell infiltration. (A) Resting memory CD4+T cells. (B) Memory B cells. (C) Plasma cells. (D) Activated memory CD4+T cells. LUSC, lung squamous cell carcinoma.

    Journal: Journal of Thoracic Disease

    Article Title: Expression of YTHDF1 and YTHDF2 in lung squamous cell carcinoma and their correlation with PD-L1

    doi: 10.21037/jtd-2026-1-0312

    Figure Lengend Snippet: Correlation of YTHDF2 expression levels with LUSC immune cell infiltration. (A) Resting memory CD4+T cells. (B) Memory B cells. (C) Plasma cells. (D) Activated memory CD4+T cells. LUSC, lung squamous cell carcinoma.

    Article Snippet: YTHDF2 antibodies were purchased from ABclonal Biotechnology Co., Ltd. (Wuhan, China), and Ethylene Glycol-bis (2-aminoethyl Ether)-N,N,N',N'-tetraacetic Acid (EGTA) antigen retrieval buffer (pH 9.0), antibody diluent, polylysine, phosphate buffered saline (PBS), super-sensitive 3,3'-diaminobenzidine (DAB) substrate, instant immunohistochemistry MaxVision TM kit, and neutral balsam were purchased from Fuzhou Maxim Biotechnology Co., Ltd. (Fuzhou, China).

    Techniques: Expressing, Clinical Proteomics

    Comparison of YTHDF1 (A) and YTHDF2 (B) expression levels in tumor tissues and adjacent tissues. IOD, integrated optical density.

    Journal: Journal of Thoracic Disease

    Article Title: Expression of YTHDF1 and YTHDF2 in lung squamous cell carcinoma and their correlation with PD-L1

    doi: 10.21037/jtd-2026-1-0312

    Figure Lengend Snippet: Comparison of YTHDF1 (A) and YTHDF2 (B) expression levels in tumor tissues and adjacent tissues. IOD, integrated optical density.

    Article Snippet: YTHDF2 antibodies were purchased from ABclonal Biotechnology Co., Ltd. (Wuhan, China), and Ethylene Glycol-bis (2-aminoethyl Ether)-N,N,N',N'-tetraacetic Acid (EGTA) antigen retrieval buffer (pH 9.0), antibody diluent, polylysine, phosphate buffered saline (PBS), super-sensitive 3,3'-diaminobenzidine (DAB) substrate, instant immunohistochemistry MaxVision TM kit, and neutral balsam were purchased from Fuzhou Maxim Biotechnology Co., Ltd. (Fuzhou, China).

    Techniques: Comparison, Expressing

    Comparison of YTHDF1 positive (A), YTHDF1 negative (B), YTHDF2 positive (C) and YTHDF2 negative (D) expression.

    Journal: Journal of Thoracic Disease

    Article Title: Expression of YTHDF1 and YTHDF2 in lung squamous cell carcinoma and their correlation with PD-L1

    doi: 10.21037/jtd-2026-1-0312

    Figure Lengend Snippet: Comparison of YTHDF1 positive (A), YTHDF1 negative (B), YTHDF2 positive (C) and YTHDF2 negative (D) expression.

    Article Snippet: YTHDF2 antibodies were purchased from ABclonal Biotechnology Co., Ltd. (Wuhan, China), and Ethylene Glycol-bis (2-aminoethyl Ether)-N,N,N',N'-tetraacetic Acid (EGTA) antigen retrieval buffer (pH 9.0), antibody diluent, polylysine, phosphate buffered saline (PBS), super-sensitive 3,3'-diaminobenzidine (DAB) substrate, instant immunohistochemistry MaxVision TM kit, and neutral balsam were purchased from Fuzhou Maxim Biotechnology Co., Ltd. (Fuzhou, China).

    Techniques: Comparison, Expressing