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1 ap  (Proteintech)


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    Proteintech 1 ap
    1 Ap, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 375 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ythdf1/pmc12856352-7-4-2?v=Proteintech
    Average 96 stars, based on 375 article reviews
    1 ap - by Bioz Stars, 2026-08
    96/100 stars

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    TXNIP translation is dependent on the m 6 A reader YTHDF1 in hASMCs (A and B) Representative western blots and quantification of TXNIP expression in YTHDF1-silenced hASMCs following AngII (1 μM) treatment ( n = 3 samples/group). (C) RT-qPCR analysis of TXNIP mRNA in YTHDF1-silenced hASMCs ( n = 3 samples/group). (D) Representative western blot showing the successful immunoprecipitation of YTHDF1 protein in RIP samples using <t>an</t> <t>anti-YTHDF1</t> antibody. Immunoglobulin G (IgG) served as the negative control ( n = 3 samples/group). (E) RT-qPCR analysis of TXNIP mRNA enrichment in RIP assays using an anti-YTHDF1 antibody ( n = 3 samples/group). Values are represented as mean ± SD; ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001 vs. sicontrol; ## p < 0.01 vs. siYTHDF1.
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    TXNIP translation is dependent on the m 6 A reader YTHDF1 in hASMCs (A and B) Representative western blots and quantification of TXNIP expression in YTHDF1-silenced hASMCs following AngII (1 μM) treatment ( n = 3 samples/group). (C) RT-qPCR analysis of TXNIP mRNA in YTHDF1-silenced hASMCs ( n = 3 samples/group). (D) Representative western blot showing the successful immunoprecipitation of YTHDF1 protein in RIP samples using <t>an</t> <t>anti-YTHDF1</t> antibody. Immunoglobulin G (IgG) served as the negative control ( n = 3 samples/group). (E) RT-qPCR analysis of TXNIP mRNA enrichment in RIP assays using an anti-YTHDF1 antibody ( n = 3 samples/group). Values are represented as mean ± SD; ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001 vs. sicontrol; ## p < 0.01 vs. siYTHDF1.
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    The results of in vitro experiment validation. ( A ) HE and Masson staining. ( B ) RT-PCR analysis of CBLL1, ELAVL1, <t>RBM15B</t> YTHDF1, METTL3 expression levels (n=3). ( C ) CBLL1, ELAVL1, RBM15B YTHDF1, METTL3 expression was detected via immunofluorescent. * P <0.05, ** P <0.01.
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    Image Search Results


    TXNIP translation is dependent on the m 6 A reader YTHDF1 in hASMCs (A and B) Representative western blots and quantification of TXNIP expression in YTHDF1-silenced hASMCs following AngII (1 μM) treatment ( n = 3 samples/group). (C) RT-qPCR analysis of TXNIP mRNA in YTHDF1-silenced hASMCs ( n = 3 samples/group). (D) Representative western blot showing the successful immunoprecipitation of YTHDF1 protein in RIP samples using an anti-YTHDF1 antibody. Immunoglobulin G (IgG) served as the negative control ( n = 3 samples/group). (E) RT-qPCR analysis of TXNIP mRNA enrichment in RIP assays using an anti-YTHDF1 antibody ( n = 3 samples/group). Values are represented as mean ± SD; ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001 vs. sicontrol; ## p < 0.01 vs. siYTHDF1.

    Journal: iScience

    Article Title: N6-methyladenosine (m 6 A) modification of TXNIP in 3′UTR instigates abdominal aorta aneurysm in mice

    doi: 10.1016/j.isci.2026.114630

    Figure Lengend Snippet: TXNIP translation is dependent on the m 6 A reader YTHDF1 in hASMCs (A and B) Representative western blots and quantification of TXNIP expression in YTHDF1-silenced hASMCs following AngII (1 μM) treatment ( n = 3 samples/group). (C) RT-qPCR analysis of TXNIP mRNA in YTHDF1-silenced hASMCs ( n = 3 samples/group). (D) Representative western blot showing the successful immunoprecipitation of YTHDF1 protein in RIP samples using an anti-YTHDF1 antibody. Immunoglobulin G (IgG) served as the negative control ( n = 3 samples/group). (E) RT-qPCR analysis of TXNIP mRNA enrichment in RIP assays using an anti-YTHDF1 antibody ( n = 3 samples/group). Values are represented as mean ± SD; ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001 vs. sicontrol; ## p < 0.01 vs. siYTHDF1.

    Article Snippet: anti-YTHDF1 , Proteintech , Cat#17479-1-AP.

    Techniques: Western Blot, Expressing, Quantitative RT-PCR, Immunoprecipitation, Negative Control

    The results of in vitro experiment validation. ( A ) HE and Masson staining. ( B ) RT-PCR analysis of CBLL1, ELAVL1, RBM15B YTHDF1, METTL3 expression levels (n=3). ( C ) CBLL1, ELAVL1, RBM15B YTHDF1, METTL3 expression was detected via immunofluorescent. * P <0.05, ** P <0.01.

    Journal: Journal of Inflammation Research

    Article Title: Machine Learning and Experimental Validation of m6A RNA Methylation Related Signatures for Risk Prediction, Diagnostic Biomarkers, and Immune Subtypes in Chronic Kidney Disease

    doi: 10.2147/JIR.S553934

    Figure Lengend Snippet: The results of in vitro experiment validation. ( A ) HE and Masson staining. ( B ) RT-PCR analysis of CBLL1, ELAVL1, RBM15B YTHDF1, METTL3 expression levels (n=3). ( C ) CBLL1, ELAVL1, RBM15B YTHDF1, METTL3 expression was detected via immunofluorescent. * P <0.05, ** P <0.01.

    Article Snippet: The primary antibodies used in the study included: METTL3 (Proteintech, 15073-1-AP), CBLL1 (Proteintech, 21179-1-AP), ELAVL1 (Proteintech, 11910-1-AP), YTHDF1 (Proteintech, 17479-1-AP), RBM15B (Proteintech, 22249-1-AP).

    Techniques: In Vitro, Biomarker Discovery, Staining, Reverse Transcription Polymerase Chain Reaction, Expressing

    Analysis of immune cell infiltration. ( A ) Heat map of correlation between the expression level of m6A-related genes and immune cell infiltration. ( B ) Analysis of the difference in immune cell content in two m6A related subtypes. Box plot showed the difference of immune cell content in different expression groups of CBLL1 ( C ), ELAVL1 ( D ), YTHDF1 ( E ), RBM15B ( F ). ns, not significant; * P <0.05, ** P <0.01, *** P <0.001.

    Journal: Journal of Inflammation Research

    Article Title: Machine Learning and Experimental Validation of m6A RNA Methylation Related Signatures for Risk Prediction, Diagnostic Biomarkers, and Immune Subtypes in Chronic Kidney Disease

    doi: 10.2147/JIR.S553934

    Figure Lengend Snippet: Analysis of immune cell infiltration. ( A ) Heat map of correlation between the expression level of m6A-related genes and immune cell infiltration. ( B ) Analysis of the difference in immune cell content in two m6A related subtypes. Box plot showed the difference of immune cell content in different expression groups of CBLL1 ( C ), ELAVL1 ( D ), YTHDF1 ( E ), RBM15B ( F ). ns, not significant; * P <0.05, ** P <0.01, *** P <0.001.

    Article Snippet: The primary antibodies used in the study included: METTL3 (Proteintech, 15073-1-AP), CBLL1 (Proteintech, 21179-1-AP), ELAVL1 (Proteintech, 11910-1-AP), YTHDF1 (Proteintech, 17479-1-AP), RBM15B (Proteintech, 22249-1-AP).

    Techniques: Expressing