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anti ythdf1  (Proteintech)


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    Structured Review

    Proteintech anti ythdf1
    Anti Ythdf1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 417 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ythdf1/YTHDF1+Antibody/pm42893454-262-8-20
    Average 96 stars, based on 417 article reviews
    anti ythdf1 - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    other:

    Article Title: Machine Learning and Experimental Validation of m6A RNA Methylation Related Signatures for Risk Prediction, Diagnostic Biomarkers, and Immune Subtypes in Chronic Kidney Disease
    Article Snippet: The primary antibodies used in the study included: METTL3 (Proteintech, 15073-1-AP), CBLL1 (Proteintech, 21179-1-AP), ELAVL1 (Proteintech, 11910-1-AP), YTHDF1 (Proteintech, 17479-1-AP), RBM15B (Proteintech, 22249-1-AP).

    Article Title: Repetitive transcranial magnetic stimulation alleviates neuropathic pain via microglial polarization by modulating the METTL3/NMDAR2B/NLRP3 pathway
    Article Snippet: Protein concentrations were determined using a bicinchoninic acid (BCA) assay kit (G2002; Servicebio).

    Article Title: METTL3-mediated m 6 A modification of CACNA1E promotes osteosarcoma progression and chemoresistance by enhancing WNT7B-mediated Ca 2+ signaling
    Article Snippet: Antibodies used were as follows: antibodies of CACNA1E (24697–1-AP), GAPDH (10494–1-AP), IGF2BP1 (22803–1-AP), IGF2BP2 (24744–1-AP), IGF2BP3 (14642–1-AP), YTHDF1 (17479–1-AP), YTHDC1 (14392–1-AP), and FLAG (20543–1-AP) from Proteintech (Shanghai, China); antibodies of N-cadherin (ab76011), Vimentin (ab8069), E-cadherin (ab231303), METTL3 (ab195352), WNT7B (ab227607), and P-gp (ab129450) from Abcam (Cambridge, MA, USA); CAMK2A antibody (#11945) from CST (Danvers, MA, USA); antibodies of CAMK2N (SAB4300530) and PLCB2 (HPA041298) from Sigma-Aldrich (St. Louis, MO, USA); antibody of PLCB4 (PA5-100855) from ThermoFisher.

    Immunostaining:

    Article Title: METTL3-based epitranscriptomic editing screening identifies functional m 6 A sites in cancers.
    Article Snippet: N-methyladenosine (mA) represents the most abundant internal RNA modification and a key regulator of gene expression.. Although individual mA regulators and sites have been linked to cancer, their transcriptome-wide functional landscape remains undefined.. Here we developed an epitranscriptomic screening platform based on targeted mA deposition to identify functional modifications in prostate and lung cancer models.

    Cell-Signaling:

    Article Title: METTL3-based epitranscriptomic editing screening identifies functional m 6 A sites in cancers.
    Article Snippet: N-methyladenosine (mA) represents the most abundant internal RNA modification and a key regulator of gene expression.. Although individual mA regulators and sites have been linked to cancer, their transcriptome-wide functional landscape remains undefined.. Here we developed an epitranscriptomic screening platform based on targeted mA deposition to identify functional modifications in prostate and lung cancer models.

    Incubation:

    Article Title: METTL3-based epitranscriptomic editing screening identifies functional m 6 A sites in cancers.
    Article Snippet: N-methyladenosine (mA) represents the most abundant internal RNA modification and a key regulator of gene expression.. Although individual mA regulators and sites have been linked to cancer, their transcriptome-wide functional landscape remains undefined.. Here we developed an epitranscriptomic screening platform based on targeted mA deposition to identify functional modifications in prostate and lung cancer models.

    Article Title: YTHDC1 Orchestrates Telomerase Assembly via Scaffold-Mediated TERT-TERC Interaction.
    Article Snippet: .. After washing with PBS, the cells were blocked with 5% BSA for 30 min and incubated with the following antibodies: Flag (1:200; Sigma- Aldrich, Germany), YTHDF1 (1:200; Proteintech, USA), YTHDF2 (1:200; Proteintech), YTHDF3 (1:200; Santa Cruz Biotechnology, USA), YTHDC1 (1:200; Cell Signaling Technology, USA), YTHDC2 (1:200; Proteintech), or coilin (1:200; Proteintech) overnight at 4°C. .. After washing with PBS, the cells were blocked with 5% BSA for 30 min and incubated with the following antibodies: Flag (1:200; Sigma- Aldrich, Germany), YTHDF1 (1:200; Proteintech, USA), YTHDF2 (1:200; Proteintech), YTHDF3 (1:200; Santa Cruz Biotechnology, USA), YTHDC1 (1:200; Cell Signaling Technology, USA), YTHDC2 (1:200; Proteintech), or coilin (1:200; Proteintech) overnight at 4°C.

    Article Title: YTHDC1 Orchestrates Telomerase Assembly via Scaffold‐Mediated TERT ‐ TERC Interaction
    Article Snippet: .. After washing with PBS, the cells were blocked with 5% BSA for 30 min and incubated with the following antibodies: Flag (1:200; Sigma‐Aldrich, Germany), YTHDF1 (1:200; Proteintech, USA), YTHDF2 (1:200; Proteintech), YTHDF3 (1:200; Santa Cruz Biotechnology, USA), YTHDC1 (1:200; Cell Signaling Technology, USA), YTHDC2 (1:200; Proteintech), or coilin (1:200; Proteintech) overnight at 4°C. ..

    Article Title: Helicobacter pylori CagA effector protein drives gastric cancer progression via m6A/DUSP6-mediated regulation of tumor glycolysis
    Article Snippet: Proteins were separated by 15% SDS-PAGE and subsequently transferred onto nitrocellulose membranes (Pall Corporation, USA). .. The membranes were blocked with 5% skim milk in Tris-buffered saline with Tween 20 (TBST) for 2 hours at room temperature, followed by overnight incubation at 4 °C with specific primary antibodies: METTL3 (15073- 1-AP, Proteintech, Wuhan, China, 1:1000), USP7 (66514-1-Ig, Proteintech, 1:1000), YTHDF2 (24744-1-AP, Proteintech, 1:3000), DUSP6 (R381008, Zenbio, Chengdu, China, 1:500), GAPDH (10494-1-AP, Proteintech, 1:5000), METTL14 (A8530, ABclonal, Wuhan, China, 1:1000), WTAP (10200-1-AP, Proteintech, 1:1000), YTHDF1 (19834-1-AP, Proteintech, 1:3000), and GFP-tag (50430-2-AP, Proteintech, 1:2000). .. After three washes with TBST, the membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies (Proteintech, 1:5000) for 1 hour at room temperature.

    Saline:

    Article Title: Helicobacter pylori CagA effector protein drives gastric cancer progression via m6A/DUSP6-mediated regulation of tumor glycolysis
    Article Snippet: Proteins were separated by 15% SDS-PAGE and subsequently transferred onto nitrocellulose membranes (Pall Corporation, USA). .. The membranes were blocked with 5% skim milk in Tris-buffered saline with Tween 20 (TBST) for 2 hours at room temperature, followed by overnight incubation at 4 °C with specific primary antibodies: METTL3 (15073- 1-AP, Proteintech, Wuhan, China, 1:1000), USP7 (66514-1-Ig, Proteintech, 1:1000), YTHDF2 (24744-1-AP, Proteintech, 1:3000), DUSP6 (R381008, Zenbio, Chengdu, China, 1:500), GAPDH (10494-1-AP, Proteintech, 1:5000), METTL14 (A8530, ABclonal, Wuhan, China, 1:1000), WTAP (10200-1-AP, Proteintech, 1:1000), YTHDF1 (19834-1-AP, Proteintech, 1:3000), and GFP-tag (50430-2-AP, Proteintech, 1:2000). .. After three washes with TBST, the membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies (Proteintech, 1:5000) for 1 hour at room temperature.



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