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Structured Review

Proteintech proteins
Proteins, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 417 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ythdf1/YTHDF1+Antibody/pm41793872-60-4-7
Average 96 stars, based on 417 article reviews
proteins - by Bioz Stars, 2026-11
96/100 stars

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Related Articles

Cell-Signaling:

Article Title: p53 hotspot mutants attenuate CTL-mediated tumor cell killing through a novel ALKBH5-YTHDF3-PD-L1 pathway.
Article Snippet: TP53 is a well-documented tumor suppressor gene frequently mutated in malignancies.. It has been demonstrated that the gain-offunction (GOF) mutation of p53 promotes the development and progression of cancers; however, its extrinsic oncogenic mechanisms are still poorly understood.. Herein, we found that the oncogenic effect of mutant p53 in vivo is dependent on the tumor immune microenvironment.

Article Title: METTL3-based epitranscriptomic editing screening identifies functional m 6 A sites in cancers.
Article Snippet: N-methyladenosine (mA) represents the most abundant internal RNA modification and a key regulator of gene expression.. Although individual mA regulators and sites have been linked to cancer, their transcriptome-wide functional landscape remains undefined.. Here we developed an epitranscriptomic screening platform based on targeted mA deposition to identify functional modifications in prostate and lung cancer models.

Incubation:

Article Title: N6-methyladenosine (m 6 A) modification of TXNIP in 3′UTR instigates abdominal aorta aneurysm in mice
Article Snippet: In summary, total RNA was extracted from hASMC, followed by the isolation of poly(A) mRNA using the GenElute mRNA Miniprep Kit (Sigma-Aldrich, MRN10). .. Dynabeads Protein A (Thermo Fischer Scientific, 100-02D) and Protein G (Thermo Fischer Scientific, 100-04D) were incubated with 2 μg of YTHDF1 antibody (Proteintech, 17479-1-AP) at 4 °C for 2 h with gentle rotation. ..

Article Title: METTL3-based epitranscriptomic editing screening identifies functional m 6 A sites in cancers.
Article Snippet: N-methyladenosine (mA) represents the most abundant internal RNA modification and a key regulator of gene expression.. Although individual mA regulators and sites have been linked to cancer, their transcriptome-wide functional landscape remains undefined.. Here we developed an epitranscriptomic screening platform based on targeted mA deposition to identify functional modifications in prostate and lung cancer models.

Article Title: Laminarin suppresses PD-L1 translation by targeting YTHDF1 to exert antitumor immunity in triple-negative breast cancer.
Article Snippet: .. Afterward, 4 μg of YTHDF1 antibody (Proteintech, Cat. No. 17479–1-AP) or isotype control IgG antibody (Proteintech, Cat. No. 30000–0-AP) was pre-coated on Protein A/G magnetic beads, followed by incubation with the corresponding lysates at 4◦C overnight. .. After magnetic separation, the supernatants were collected, and total RNA was extracted using the RNAeasy Animal RNA Extraction Kit (Beyotime, Cat. No. R0027).

Gentle:

Article Title: N6-methyladenosine (m 6 A) modification of TXNIP in 3′UTR instigates abdominal aorta aneurysm in mice
Article Snippet: In summary, total RNA was extracted from hASMC, followed by the isolation of poly(A) mRNA using the GenElute mRNA Miniprep Kit (Sigma-Aldrich, MRN10). .. Dynabeads Protein A (Thermo Fischer Scientific, 100-02D) and Protein G (Thermo Fischer Scientific, 100-04D) were incubated with 2 μg of YTHDF1 antibody (Proteintech, 17479-1-AP) at 4 °C for 2 h with gentle rotation. ..

Immunostaining:

Article Title: METTL3-based epitranscriptomic editing screening identifies functional m 6 A sites in cancers.
Article Snippet: N-methyladenosine (mA) represents the most abundant internal RNA modification and a key regulator of gene expression.. Although individual mA regulators and sites have been linked to cancer, their transcriptome-wide functional landscape remains undefined.. Here we developed an epitranscriptomic screening platform based on targeted mA deposition to identify functional modifications in prostate and lung cancer models.

Control:

Article Title: Laminarin suppresses PD-L1 translation by targeting YTHDF1 to exert antitumor immunity in triple-negative breast cancer.
Article Snippet: .. Afterward, 4 μg of YTHDF1 antibody (Proteintech, Cat. No. 17479–1-AP) or isotype control IgG antibody (Proteintech, Cat. No. 30000–0-AP) was pre-coated on Protein A/G magnetic beads, followed by incubation with the corresponding lysates at 4◦C overnight. .. After magnetic separation, the supernatants were collected, and total RNA was extracted using the RNAeasy Animal RNA Extraction Kit (Beyotime, Cat. No. R0027).

Magnetic Beads:

Article Title: Laminarin suppresses PD-L1 translation by targeting YTHDF1 to exert antitumor immunity in triple-negative breast cancer.
Article Snippet: .. Afterward, 4 μg of YTHDF1 antibody (Proteintech, Cat. No. 17479–1-AP) or isotype control IgG antibody (Proteintech, Cat. No. 30000–0-AP) was pre-coated on Protein A/G magnetic beads, followed by incubation with the corresponding lysates at 4◦C overnight. .. After magnetic separation, the supernatants were collected, and total RNA was extracted using the RNAeasy Animal RNA Extraction Kit (Beyotime, Cat. No. R0027).



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