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compound wp1066 s2796  (Selleck Chemicals)


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    Selleck Chemicals compound wp1066 s2796
    FCGBP promotes glioma growth by activating the JAK2/STAT3 signaling pathway. (A) WB analysis was performed to evaluate the expression levels of JAK2, p‐JAK2, STAT3, p‐STAT3, and c‐Myc in Vector, oeFCGBP, Vector + <t>WP1066,</t> and oeFCGBP + WP1066 groups. (B) Cell growth curves of different treatment groups were measured using the CCK‐8 assay. (C) EdU incorporation assay and corresponding histogram quantification were used to evaluate cell proliferation across different treatment conditions. Data were mean ± SD. Statistical significance was calculated by 2‐way ANOVA for A–C. ** p < 0.01, *** p < 0.001.
    Compound Wp1066 S2796, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 96/100, based on 1279 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wp1066/Compound/pmc12906970-32-1-7
    Average 96 stars, based on 1279 article reviews
    compound wp1066 s2796 - by Bioz Stars, 2026-10
    96/100 stars

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    1) Product Images from "FCGBP Promotes Glioma Growth by Regulating JAK2 / STAT3 /c‐Myc Pathway"

    Article Title: FCGBP Promotes Glioma Growth by Regulating JAK2 / STAT3 /c‐Myc Pathway

    Journal: Cancer Medicine

    doi: 10.1002/cam4.71617

    FCGBP promotes glioma growth by activating the JAK2/STAT3 signaling pathway. (A) WB analysis was performed to evaluate the expression levels of JAK2, p‐JAK2, STAT3, p‐STAT3, and c‐Myc in Vector, oeFCGBP, Vector + WP1066, and oeFCGBP + WP1066 groups. (B) Cell growth curves of different treatment groups were measured using the CCK‐8 assay. (C) EdU incorporation assay and corresponding histogram quantification were used to evaluate cell proliferation across different treatment conditions. Data were mean ± SD. Statistical significance was calculated by 2‐way ANOVA for A–C. ** p < 0.01, *** p < 0.001.
    Figure Legend Snippet: FCGBP promotes glioma growth by activating the JAK2/STAT3 signaling pathway. (A) WB analysis was performed to evaluate the expression levels of JAK2, p‐JAK2, STAT3, p‐STAT3, and c‐Myc in Vector, oeFCGBP, Vector + WP1066, and oeFCGBP + WP1066 groups. (B) Cell growth curves of different treatment groups were measured using the CCK‐8 assay. (C) EdU incorporation assay and corresponding histogram quantification were used to evaluate cell proliferation across different treatment conditions. Data were mean ± SD. Statistical significance was calculated by 2‐way ANOVA for A–C. ** p < 0.01, *** p < 0.001.

    Techniques Used: Expressing, Plasmid Preparation, CCK-8 Assay

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    Article Snippet: Chemical compound, drug , MG132 , Selleck , Cat# S2619 , .

    Drug discovery:

    Article Title: Deciphering the Role of Cancer Stem Cells in Breast Cancer Brain Colonization Using a Novel Patient-Derived Model.
    Article Snippet: .. .16 Drug Screening SCs were screened with a 295-compound bioactive library, ssembled from commercial collections (Lopac 1280, Selleck pigenetics, MicoSource 2010; Table S3) using Tecan Freedom VO and CellTiter-Glo 3D assays. ..

    Article Title: Single-Cell Profiling Reveals Developmental Trajectories and identifies SYK and TIM3 as Targets in some T Cell Lymphomas
    Article Snippet: .. Two commercially-available compound libraries were purchased from suppliers and used for high-throughput in vitro drug screens: the DiscoveryProbeTM Epigenetics Compound library (281 compounds; APExBIO, TX), and the FDA Approved Drug Screening Library (1430 compounds, Selleck Chemicals, TX). .. Biomek Software (v4.1) was used to program the liquid handling robot (Beckman-Coulter NXp Laboratory Automation Workstation).

    In Vitro:

    Article Title: Single-Cell Profiling Reveals Developmental Trajectories and identifies SYK and TIM3 as Targets in some T Cell Lymphomas
    Article Snippet: .. Two commercially-available compound libraries were purchased from suppliers and used for high-throughput in vitro drug screens: the DiscoveryProbeTM Epigenetics Compound library (281 compounds; APExBIO, TX), and the FDA Approved Drug Screening Library (1430 compounds, Selleck Chemicals, TX). .. Biomek Software (v4.1) was used to program the liquid handling robot (Beckman-Coulter NXp Laboratory Automation Workstation).



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    FCGBP promotes glioma growth by activating the JAK2/STAT3 signaling pathway. (A) WB analysis was performed to evaluate the expression levels of JAK2, p‐JAK2, STAT3, p‐STAT3, and c‐Myc in Vector, oeFCGBP, Vector + <t>WP1066,</t> and oeFCGBP + WP1066 groups. (B) Cell growth curves of different treatment groups were measured using the CCK‐8 assay. (C) EdU incorporation assay and corresponding histogram quantification were used to evaluate cell proliferation across different treatment conditions. Data were mean ± SD. Statistical significance was calculated by 2‐way ANOVA for A–C. ** p < 0.01, *** p < 0.001.
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    Image Search Results


    Verification of oxidative stress in RPE cells. ( A ) Immunofluorescence double staining showing the co-localization of SPP1 and the RPE cell marker RPE65; ( B ) Immunofluorescence double staining showing the co-localization of SPP1 and the microglial marker IBA1; ( C-D ) Immunofluorescence detection of RPE cell markers (RPE65, BEST1) under oxidative stress and SPP1 knockdown conditions; ( E ) ROS levels in the Control and H 2 O 2 groups; ( F ) GSH, SOD, and GPx levels in the Control and H 2 O 2 groups, ( G ) JAK2, STAT3, p-JAK2, and p-STAT3 levels in the Control, H 2 O 2 groups detected by WB; (H) Protein quantification of JAK2, STAT3, p-JAK2 and p-STAT3 in each group; ( I ) The survival rate of ARPE-19 cells in the Control, H 2 O 2 , H 2 O 2 +WP1066, and H 2 O 2 +IFN-γ groups detected by CCK-8.

    Journal: Scientific Reports

    Article Title: Oxidative stress promotes the progression of age-related macular degeneration by up-regulating Spp1 to activate the JAK/STAT signaling pathway

    doi: 10.1038/s41598-026-48556-6

    Figure Lengend Snippet: Verification of oxidative stress in RPE cells. ( A ) Immunofluorescence double staining showing the co-localization of SPP1 and the RPE cell marker RPE65; ( B ) Immunofluorescence double staining showing the co-localization of SPP1 and the microglial marker IBA1; ( C-D ) Immunofluorescence detection of RPE cell markers (RPE65, BEST1) under oxidative stress and SPP1 knockdown conditions; ( E ) ROS levels in the Control and H 2 O 2 groups; ( F ) GSH, SOD, and GPx levels in the Control and H 2 O 2 groups, ( G ) JAK2, STAT3, p-JAK2, and p-STAT3 levels in the Control, H 2 O 2 groups detected by WB; (H) Protein quantification of JAK2, STAT3, p-JAK2 and p-STAT3 in each group; ( I ) The survival rate of ARPE-19 cells in the Control, H 2 O 2 , H 2 O 2 +WP1066, and H 2 O 2 +IFN-γ groups detected by CCK-8.

    Article Snippet: In the Control group, ARPE-19 cells were cultured with complete medium; In the H 2 O 2 group, ARPE-19 cells were induced with H 2 O 2 (200 μM) for 24 h. In the inhibitor group, H 2 O 2 -induced ARPE-19 cells were treated with WP1066 (MCE, HY-15312).

    Techniques: Immunofluorescence, Double Staining, Marker, Knockdown, Control, CCK-8 Assay

    Verification of the effect of SPP1 expression on apoptosis of RPE cells. ( A ) The expression of SPP1 in ARPE-19 cells in the Control and H 2 O 2 groups detected by WB; ( B ) The expression of SPP1 in ARPE-19 cells in the H 2 O 2 and H 2 O 2 +si-SPP1 groups detected by qRT-PCR; ( C ) The knockdown efficiency of SPP1 was verified by qRT-PCR; ( D )ROS levels in the H 2 O 2 and H 2 O 2 +si-SPP1 groups; ( E ) Gsh, SOD and GPx levels in the H 2 O 2 and H 2 O 2 +si-SPP1 groups; ( F ) The expression of CD44 in the H 2 O 2 group and the H 2 O 2 +si-SPP1 group detected by WB; ( G ) The levels of JAK2, STAT3, p-JAK2 and p-STAT3 in the H 2 O 2 and H 2 O 2 +si-SPP1 groups detected by WB. ( H ) Protein quantification of JAK2, STAT3, p-JAK2 and p-STAT3 in each group; ( I ) The survival rate of ARPE-19 cells in the H 2 O 2 , H 2 O 2 +si-SPP1, H 2 O 2 +si-SPP1 + reco-SPP1, H 2 O 2 +WP1066, and H 2 O 2 +IFN-γ groups detected by CCK-8. Compared with the control group, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001; # # P < 0.01.

    Journal: Scientific Reports

    Article Title: Oxidative stress promotes the progression of age-related macular degeneration by up-regulating Spp1 to activate the JAK/STAT signaling pathway

    doi: 10.1038/s41598-026-48556-6

    Figure Lengend Snippet: Verification of the effect of SPP1 expression on apoptosis of RPE cells. ( A ) The expression of SPP1 in ARPE-19 cells in the Control and H 2 O 2 groups detected by WB; ( B ) The expression of SPP1 in ARPE-19 cells in the H 2 O 2 and H 2 O 2 +si-SPP1 groups detected by qRT-PCR; ( C ) The knockdown efficiency of SPP1 was verified by qRT-PCR; ( D )ROS levels in the H 2 O 2 and H 2 O 2 +si-SPP1 groups; ( E ) Gsh, SOD and GPx levels in the H 2 O 2 and H 2 O 2 +si-SPP1 groups; ( F ) The expression of CD44 in the H 2 O 2 group and the H 2 O 2 +si-SPP1 group detected by WB; ( G ) The levels of JAK2, STAT3, p-JAK2 and p-STAT3 in the H 2 O 2 and H 2 O 2 +si-SPP1 groups detected by WB. ( H ) Protein quantification of JAK2, STAT3, p-JAK2 and p-STAT3 in each group; ( I ) The survival rate of ARPE-19 cells in the H 2 O 2 , H 2 O 2 +si-SPP1, H 2 O 2 +si-SPP1 + reco-SPP1, H 2 O 2 +WP1066, and H 2 O 2 +IFN-γ groups detected by CCK-8. Compared with the control group, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001; # # P < 0.01.

    Article Snippet: In the Control group, ARPE-19 cells were cultured with complete medium; In the H 2 O 2 group, ARPE-19 cells were induced with H 2 O 2 (200 μM) for 24 h. In the inhibitor group, H 2 O 2 -induced ARPE-19 cells were treated with WP1066 (MCE, HY-15312).

    Techniques: Expressing, Control, Quantitative RT-PCR, Knockdown, CCK-8 Assay

    FCGBP promotes glioma growth by activating the JAK2/STAT3 signaling pathway. (A) WB analysis was performed to evaluate the expression levels of JAK2, p‐JAK2, STAT3, p‐STAT3, and c‐Myc in Vector, oeFCGBP, Vector + WP1066, and oeFCGBP + WP1066 groups. (B) Cell growth curves of different treatment groups were measured using the CCK‐8 assay. (C) EdU incorporation assay and corresponding histogram quantification were used to evaluate cell proliferation across different treatment conditions. Data were mean ± SD. Statistical significance was calculated by 2‐way ANOVA for A–C. ** p < 0.01, *** p < 0.001.

    Journal: Cancer Medicine

    Article Title: FCGBP Promotes Glioma Growth by Regulating JAK2 / STAT3 /c‐Myc Pathway

    doi: 10.1002/cam4.71617

    Figure Lengend Snippet: FCGBP promotes glioma growth by activating the JAK2/STAT3 signaling pathway. (A) WB analysis was performed to evaluate the expression levels of JAK2, p‐JAK2, STAT3, p‐STAT3, and c‐Myc in Vector, oeFCGBP, Vector + WP1066, and oeFCGBP + WP1066 groups. (B) Cell growth curves of different treatment groups were measured using the CCK‐8 assay. (C) EdU incorporation assay and corresponding histogram quantification were used to evaluate cell proliferation across different treatment conditions. Data were mean ± SD. Statistical significance was calculated by 2‐way ANOVA for A–C. ** p < 0.01, *** p < 0.001.

    Article Snippet: The compound WP1066 (S2796) was sourced from Selleck, China.

    Techniques: Expressing, Plasmid Preparation, CCK-8 Assay