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well cell culture plates  (R&D Systems)


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    R&D Systems well cell culture plates
    Well Cell Culture Plates, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vitronectin/Recombinant+Human+Vitronectin+Protein%2C+CF/pmc12548079-237-1-9
    Average 93 stars, based on 16 article reviews
    well cell culture plates - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Bi-functional integrin degraders cause stronger anti-proliferative effects than a blocking antibody by modulating distinct downstream signaling
    Article Snippet: .. The 24-well cell culture plates were coated with vitronectin (R&D Systems 2308-VN; 20 μg/well; 1 mL PBS/well) by incubating for 1 hour at 37°C. .. PBS (Cytiva, SH30256.01) was aspirated from each well and HEPG2 cells were plated (100,000 cells/well) and incubated overnight at 37°C.

    Article Title: Bi-functional integrin degraders cause stronger anti-proliferative effects than a blocking antibody by modulating distinct downstream signaling
    Article Snippet: HEPG2 (ATCC, HB-8065) cells were grown and maintained in T75 flasks (Corning, 430,641 U) at 37°C and 5% CO 2 in DMEM (Gibco, 11,995–065) supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS; Gibco, A38401) and 1% penicillin/streptomycin (Gibco, 15,140,122). .. The 6-well cell culture plates were coated with vitronectin (R&D Systems 2308-VN; 100 μg/well; 1 mL phosphate-buffered saline (PBS)/well) by incubating for 1 hour at 37°C. .. PBS (Cytiva, SH30256.01) was aspirated from each well and HEPG2 cells were plated (350,000 cells per well) and incubated overnight.

    Article Title: Bi-functional integrin degraders cause stronger anti-proliferative effects than a blocking antibody by modulating distinct downstream signaling
    Article Snippet: .. The 6-well cell culture plates were coated with vitronectin (R&D Systems 2308-VN; 100 μg/well; 1 mL PBS/well) by incubating for 1 hour at 37°C. ..

    Recombinant:

    Article Title: Use of a Recombinant Biomarker Protein DDA Library Increases DIA Coverage of Low Abundance Plasma Proteins.
    Article Snippet: .. Recombinant proteins ADEMDEC1 (TP721090), BTC (TP723036), C1QC (TP761200), CPQ (TP760108), CXCL10 (IP10, TP723726), CXCL8 (IL8, TP721122), MMP2 (TP723320), MUC1 (TP760771), PDGFB (TP723355), TGFA (TP723858), TIMP2 (TP723886) and TNFRSF1A (TP723870) were purchased from OriGene Technologies, CEACAM5 (4128-CM), CST3 (1196-PI), CXCL12 (350-NS), EGFR (1095-ER), IL1B (201-LB), IL6 (206-IL), KLK3 (1344-SE), MIA (9250-IA), MMP3 (513- MP), MMP9 (911-MP), PLAU (1310-SE), PLAUR (807- UK), PTEN (847-PN), S100A8/A9 (8226-S8), TIMP1 (970- TM),TNF (210-TA), TP53 (SP-454) and VN (2308-VN) from R&D Systems, PFN1 (NBP1-30215), PLG (H00005340P01), ITGAV (H00003685-P01), ITGB1 (H00003688-P01) and ITGB6 (H00003694-P01) from Novus Biologicals and EGF (MBS650012) from My BioSource. ..

    Article Title: Netrin-4 promotes mural cell adhesion and recruitment to endothelial cells
    Article Snippet: .. The wells of 96-well Maxisorp plates (Nunc) were coated overnight at 37°C with either 1% BSA (Bovine serum Albumin, Sigma), 2.5 μg/ml human Vitronectin (R&D) or recombinant human Netrin-4 (R&D) diluted in PBS. ..

    Saline:

    Article Title: Bi-functional integrin degraders cause stronger anti-proliferative effects than a blocking antibody by modulating distinct downstream signaling
    Article Snippet: HEPG2 (ATCC, HB-8065) cells were grown and maintained in T75 flasks (Corning, 430,641 U) at 37°C and 5% CO 2 in DMEM (Gibco, 11,995–065) supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS; Gibco, A38401) and 1% penicillin/streptomycin (Gibco, 15,140,122). .. The 6-well cell culture plates were coated with vitronectin (R&D Systems 2308-VN; 100 μg/well; 1 mL phosphate-buffered saline (PBS)/well) by incubating for 1 hour at 37°C. .. PBS (Cytiva, SH30256.01) was aspirated from each well and HEPG2 cells were plated (350,000 cells per well) and incubated overnight.



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    (A) Representative confocal immunofluorescence images showing ApoE and PLIN2 staining (green). Scale bar = 30 µm. Lamp2a -/- mice showed increased staining of both proteins compared with WT mice, which was markedly reduced by GLA-1-1 treatment. (B) Bar graph showing quantification of ApoE fluorescence intensity (mean gray value) (n = 7 mice). (C) Bar graph showing quantification of PLIN2 immunofluorescence signal (n = 5 mice). (D) Representative confocal immunofluorescence images showing staining for <t>vitronectin,</t> MMP2, and clusterin. Scale bar = 30 µm. Compared with WT mice, Lamp2a -/- mice showed markedly increased immunoreactivity for these proteins, which was significantly reduced by GLA-1-1 treatment. (E) Bar graph showing quantification of MMP2 fluorescence intensity (mean gray value) (n=5 mice). (F) Bar graph showing quantification of vitronectin fluorescence intensity (mean gray value) (n=7 mice). (G) Bar graph showing quantification of clusterin fluorescence intensity (mean gray value) (n=7 mice). (H) Representative immunoblots showing protein levels of ApoE, clusterin, vitronectin, and PLIN2 in RPE/choroid lysates. Bar graphs showing immunoblot quantification of protein levels of vitronectin (I), clusterin (J), ApoE (K), and PLIN2 (L) in RPE/choroid lysates (n = 3 mice per group). Statistical analysis was performed using one-way ANOVA with Tukey’s post hoc test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Values are expressed as mean ± SD.
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    Image Search Results


    (A) Representative confocal immunofluorescence images showing ApoE and PLIN2 staining (green). Scale bar = 30 µm. Lamp2a -/- mice showed increased staining of both proteins compared with WT mice, which was markedly reduced by GLA-1-1 treatment. (B) Bar graph showing quantification of ApoE fluorescence intensity (mean gray value) (n = 7 mice). (C) Bar graph showing quantification of PLIN2 immunofluorescence signal (n = 5 mice). (D) Representative confocal immunofluorescence images showing staining for vitronectin, MMP2, and clusterin. Scale bar = 30 µm. Compared with WT mice, Lamp2a -/- mice showed markedly increased immunoreactivity for these proteins, which was significantly reduced by GLA-1-1 treatment. (E) Bar graph showing quantification of MMP2 fluorescence intensity (mean gray value) (n=5 mice). (F) Bar graph showing quantification of vitronectin fluorescence intensity (mean gray value) (n=7 mice). (G) Bar graph showing quantification of clusterin fluorescence intensity (mean gray value) (n=7 mice). (H) Representative immunoblots showing protein levels of ApoE, clusterin, vitronectin, and PLIN2 in RPE/choroid lysates. Bar graphs showing immunoblot quantification of protein levels of vitronectin (I), clusterin (J), ApoE (K), and PLIN2 (L) in RPE/choroid lysates (n = 3 mice per group). Statistical analysis was performed using one-way ANOVA with Tukey’s post hoc test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Values are expressed as mean ± SD.

    Journal: bioRxiv

    Article Title: Loss of Lamp2a-dependent chaperone-mediated autophagy drives dry AMD-like retinal pathology in mice and is rescued by BK channel activation

    doi: 10.64898/2026.03.19.712761

    Figure Lengend Snippet: (A) Representative confocal immunofluorescence images showing ApoE and PLIN2 staining (green). Scale bar = 30 µm. Lamp2a -/- mice showed increased staining of both proteins compared with WT mice, which was markedly reduced by GLA-1-1 treatment. (B) Bar graph showing quantification of ApoE fluorescence intensity (mean gray value) (n = 7 mice). (C) Bar graph showing quantification of PLIN2 immunofluorescence signal (n = 5 mice). (D) Representative confocal immunofluorescence images showing staining for vitronectin, MMP2, and clusterin. Scale bar = 30 µm. Compared with WT mice, Lamp2a -/- mice showed markedly increased immunoreactivity for these proteins, which was significantly reduced by GLA-1-1 treatment. (E) Bar graph showing quantification of MMP2 fluorescence intensity (mean gray value) (n=5 mice). (F) Bar graph showing quantification of vitronectin fluorescence intensity (mean gray value) (n=7 mice). (G) Bar graph showing quantification of clusterin fluorescence intensity (mean gray value) (n=7 mice). (H) Representative immunoblots showing protein levels of ApoE, clusterin, vitronectin, and PLIN2 in RPE/choroid lysates. Bar graphs showing immunoblot quantification of protein levels of vitronectin (I), clusterin (J), ApoE (K), and PLIN2 (L) in RPE/choroid lysates (n = 3 mice per group). Statistical analysis was performed using one-way ANOVA with Tukey’s post hoc test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Values are expressed as mean ± SD.

    Article Snippet: Antibodies against LC3B (Sigma, L8918-200UL), actin (Proteintech, 66009-1-Ig), PLIN2 (Proteintech, 15294-1-AP), fibronectin (Invitrogen, PA5-29578), clusterin (R&D systems, AF2747), vitronectin (R&D Systems, MAB38751), and p62 (Abnova, H00008878-M01; CST, 5114), Lamp2a (Abcam, ab125068), Lamp2b (Abcam, ab13524), Iba1 (Fujifilm Wako, 019-19741), Laminin (Sigma, L9393) and ApoE (Abcam, ab183596) were used in this study.

    Techniques: Immunofluorescence, Staining, Fluorescence, Western Blot