Journal: Heliyon

Article Title: Follicular fluid aids cell adhesion, spreading in an age independent manner and shows an age-dependent effect on DNA damage in fallopian tube epithelial cells

doi: 10.1016/j.heliyon.2024.e27336

Figure Lengend Snippet: Proteomic analysis of FF samples revealed extracellular matrix (ECM) protein - vitronectin as the component responsible for FTE adhesion and spreading A. Experimental workflow of proteomics experiment. B. Venn diagram showing 14 common proteins identified between young and aged FF samples from proteomics analysis. Common proteins are listed with vitronectin (highlighted in red). C. Representative immunoblot for vitronectin expression in 3 young (Y1–Y3) and 3 aged (A1-A3) FF samples. FF samples (5 μg) and recombinant vitronectin protein (0.01 μg, 0.1 μg, 1 μg) diluted with lysis buffer were used for immunoblotting. Arrow represents the band analyzed for vitronectin. Ponceau staining was used for loading control.

Article Snippet: Anti-rabbit vitronectin , Proteintech 15833-1-AP , 1:1000.

Techniques: Western Blot, Expressing, Recombinant, Lysis, Staining, Control

Journal: Heliyon

Article Title: Follicular fluid aids cell adhesion, spreading in an age independent manner and shows an age-dependent effect on DNA damage in fallopian tube epithelial cells

doi: 10.1016/j.heliyon.2024.e27336

Figure Lengend Snippet: Vitronectin in FF aids in FTE adhesion and spreading A. Representative brightfield images of FT190 cells seeded on ULA plates coated with FF sample (400 μl) and recombinant vitronectin protein (1 μg/well) for 4 h. Wells were washed with 1X PBS and FT190 cells were seeded on the coated plates. Images were acquired after 24 h. Scale bar = 200 μm. B. Cell proliferation was measured using an SRB assay to measure cell viability of FT190 cells on FF and vitronectin coated ULA plates. C. Representative Z stack images acquired by confocal microscopy of FT190 spheroids (labelled with Cell tracker 594) on NOF151 cells (labelled with Cell tracker 488) with FF samples and recombinant vitronectin protein (1 μg) (24 h). Scale bar = 200 μm. D. A maximum intensity projection re-construction of a three-dimensional FTE spheroid (labelled with Cell tracker 594) optical data stack over the surface of NOF151 cells (labelled with Cell tracker 488) treated with FF samples (Y1, A1) and vitronectin (1 μg). Spheroids were imaged with a 10× objective and re-constructed using the Imaris software. E. Representative images acquired by confocal microscopy showing a side and top projection of the FT190 spheroids on NOF151 cells with FF samples and recombinant vitronectin protein (1 μg) (24 h). Scale bar = 100 μm. F. Spheroid area (with and without FF/vitronectin) was determined by quantification of red fluorescent intensity using a polygon area measurement tool of the Imaris software.

Article Snippet: Anti-rabbit vitronectin , Proteintech 15833-1-AP , 1:1000.

Techniques: Recombinant, Sulforhodamine B Assay, Confocal Microscopy, Construct, Software

Journal: Heliyon

Article Title: Follicular fluid aids cell adhesion, spreading in an age independent manner and shows an age-dependent effect on DNA damage in fallopian tube epithelial cells

doi: 10.1016/j.heliyon.2024.e27336

Figure Lengend Snippet: Primary antibodies.

Article Snippet: Anti-rabbit vitronectin , Proteintech 15833-1-AP , 1:1000.

Techniques:

Journal: Stroke

Article Title: Blood vitronectin induces detrimental brain IL-6 and correlates with outcomes after stroke only in female mice

doi: 10.1161/STROKEAHA.120.029036

Figure Lengend Snippet: A) Plasma VTN concentrations were increased 24 hours after compared to 24 hours before MCAO in female, but not male, VTN+/+ (N=10,7,10,12) and C57BL/6 mice (N=13,11). B) At 7 days, plasma VTN returned to baseline levels in females, but MCAO did not change plasma VTN in males (N= 6,7, repeated measures). C) Plasma VTN concentrations in female, but not male, C57BL/6 mice at 24 hours after MCAO correlated to injury size at 7 days, measured in GFAP-stained sections (N=6,7). D) MCAO increased plasma VTN at 24 hours compared to both naïve and sham operated C57BL/6 female mice (N=6,7,4). * p<0.05, ** p<0.01, *** p<0.001, **** or #### p<0.0001

Article Snippet: Plasma VTN concentrations were measured by ELISA (MVNKT-TOT, Molecular Innovations) and in the injured striatum by western blot.

Techniques: Staining

Journal: Stroke

Article Title: Blood vitronectin induces detrimental brain IL-6 and correlates with outcomes after stroke only in female mice

doi: 10.1161/STROKEAHA.120.029036

Figure Lengend Snippet: At 14 days after MCAO, VTN−/− female mice showed less A) CD68-positive activated microglia/macrophages and B) CD45-positive infiltrated leukocytes than VTN+/+ females, as shown by area and density measurements (N=6,5; * p<0.05, ** p<0.01). Dashed lines delineate injured areas with dense immunostained cells. C) Plasma VTN concentrations in female C57BL/6 mice at 24 hours correlated to CD68 and CD45 density at 7 days after MCAO (N=6). D) No correlation was present in males (N=7).

Article Snippet: Plasma VTN concentrations were measured by ELISA (MVNKT-TOT, Molecular Innovations) and in the injured striatum by western blot.

Techniques:

Journal: Stroke

Article Title: Blood vitronectin induces detrimental brain IL-6 and correlates with outcomes after stroke only in female mice

doi: 10.1161/STROKEAHA.120.029036

Figure Lengend Snippet: A) In VTN−/− females, MCAO-induced IL-6 in the injured striatum at 24 hours after MCAO was much less compared to VTN+/+ females. There were no genotype differences in MCAO-induced ciliary neurotrophic factor (CNTF) or leukemia inhibitory factor (LIF). N=5,7,6,6, * or # p<0.05, ** p<0.01. B) Males did not show genotype differences in MCAO-induced IL-6, CNTF or LIF (N=6,10,8,11). Plasma VTN concentrations correlated with IL-6, CNTF and LIF expression in females C), but not males D), N=11/group.

Article Snippet: Plasma VTN concentrations were measured by ELISA (MVNKT-TOT, Molecular Innovations) and in the injured striatum by western blot.

Techniques: Expressing