Journal: Heliyon

Article Title: Follicular fluid aids cell adhesion, spreading in an age independent manner and shows an age-dependent effect on DNA damage in fallopian tube epithelial cells

doi: 10.1016/j.heliyon.2024.e27336

Figure Lengend Snippet: Proteomic analysis of FF samples revealed extracellular matrix (ECM) protein - vitronectin as the component responsible for FTE adhesion and spreading A. Experimental workflow of proteomics experiment. B. Venn diagram showing 14 common proteins identified between young and aged FF samples from proteomics analysis. Common proteins are listed with vitronectin (highlighted in red). C. Representative immunoblot for vitronectin expression in 3 young (Y1–Y3) and 3 aged (A1-A3) FF samples. FF samples (5 μg) and recombinant vitronectin protein (0.01 μg, 0.1 μg, 1 μg) diluted with lysis buffer were used for immunoblotting. Arrow represents the band analyzed for vitronectin. Ponceau staining was used for loading control.

Article Snippet: Anti-rabbit vitronectin , Proteintech 15833-1-AP , 1:1000.

Techniques: Western Blot, Expressing, Recombinant, Lysis, Staining, Control

Journal: Heliyon

Article Title: Follicular fluid aids cell adhesion, spreading in an age independent manner and shows an age-dependent effect on DNA damage in fallopian tube epithelial cells

doi: 10.1016/j.heliyon.2024.e27336

Figure Lengend Snippet: Vitronectin in FF aids in FTE adhesion and spreading A. Representative brightfield images of FT190 cells seeded on ULA plates coated with FF sample (400 μl) and recombinant vitronectin protein (1 μg/well) for 4 h. Wells were washed with 1X PBS and FT190 cells were seeded on the coated plates. Images were acquired after 24 h. Scale bar = 200 μm. B. Cell proliferation was measured using an SRB assay to measure cell viability of FT190 cells on FF and vitronectin coated ULA plates. C. Representative Z stack images acquired by confocal microscopy of FT190 spheroids (labelled with Cell tracker 594) on NOF151 cells (labelled with Cell tracker 488) with FF samples and recombinant vitronectin protein (1 μg) (24 h). Scale bar = 200 μm. D. A maximum intensity projection re-construction of a three-dimensional FTE spheroid (labelled with Cell tracker 594) optical data stack over the surface of NOF151 cells (labelled with Cell tracker 488) treated with FF samples (Y1, A1) and vitronectin (1 μg). Spheroids were imaged with a 10× objective and re-constructed using the Imaris software. E. Representative images acquired by confocal microscopy showing a side and top projection of the FT190 spheroids on NOF151 cells with FF samples and recombinant vitronectin protein (1 μg) (24 h). Scale bar = 100 μm. F. Spheroid area (with and without FF/vitronectin) was determined by quantification of red fluorescent intensity using a polygon area measurement tool of the Imaris software.

Article Snippet: Anti-rabbit vitronectin , Proteintech 15833-1-AP , 1:1000.

Techniques: Recombinant, Sulforhodamine B Assay, Confocal Microscopy, Construct, Software

Journal: Heliyon

Article Title: Follicular fluid aids cell adhesion, spreading in an age independent manner and shows an age-dependent effect on DNA damage in fallopian tube epithelial cells

doi: 10.1016/j.heliyon.2024.e27336

Figure Lengend Snippet: Primary antibodies.

Article Snippet: Anti-rabbit vitronectin , Proteintech 15833-1-AP , 1:1000.

Techniques:

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Biological boundary conditions regulate the internalization of Aspergillus fumigatus conidia by alveolar cells

doi: 10.3389/fcimb.2025.1515779

Figure Lengend Snippet: Internalization of A fumigatus conidia in a subpopulation of Lamp + Actin + vesicles in cells on circular micropatterns and effect of ECM composition on conidia internalization. (A) Representative example of two cells on fibronectin-coated circular micropattern (28 µm). Overview of Lamp1 and Actin is shown as maximal Z-projection of FITC/TRITC channels of 9 focal planes. Magnified images show conidia in Lamp1 + (green) and Lamp1 + Actin + vesicles (red and green) 3 hours post-infection. Scale bars = 10 µm. (B) Percentage of conidia internalized in Lamp1 + Actin + vesicles relative to the total conidia number in cells constrained on fibronectin-coated circular micropatterns with different sizes and cell density at 1 and 3 hours post-infection. (C) Conidia spatial distribution map of 50 overlaid micropatterns displaying Lamp1 + Actin + vesicles with internalized conidia (green spheres in red circles). (D) Comparison of vitronectin- and fibronectin micropatterns. Quantification of the average number of total conidia and the percentage of conidia internalized in Lamp1 + and Lamp1 + Actin + vesicles at 1 and 3 hours post-infection relative to the total conidia number. **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001; ns P > 0.05. VN, vitronectin; FN, fibronectin. See also Supplementary Figure 1 .

Article Snippet: The patterned substrates were subsequently incubated with 150 μl of fibronectin (10 μg/ml, Sigma Aldrich, 341631, Austria) in PBS or vitronectin (3.3 μl/ml, ProSci, 91-362, USA) at 4°C overnight and washed once with PBS.

Techniques: Infection, Comparison