two-color gene expression microarray platform (Agilent technologies)
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Agilent technologies
two-color gene expression microarray platform
Two Color Gene Expression Microarray Platform, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/two-color+microarray+platform/pmc05522607-99-13-12
Average 90 stars, based on 1 article reviews
Two Color Gene Expression Microarray Platform, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/two-color+microarray+platform/pmc05522607-99-13-12
Average 90 stars, based on 1 article reviews
two-color gene expression microarray platform - by Bioz Stars,
2026-09
90/100 stars
Images
1) Product Images from "Identifying genes and regulatory pathways associated with the scleractinian coral calcification process"
Article Title: Identifying genes and regulatory pathways associated with the scleractinian coral calcification process
Journal: PeerJ
doi: 10.7717/peerj.3590
Figure Legend Snippet: Eight fragments of Stylophora pistillata were incubated in calcium seawater (SW) concentrations of seawater plus 100 mg/L calcium (SW + 100) and plus 200 mg/L calcium (SW + 200) during daytime (11:00) and during night-time (23:00). In each sampling time, four replicates were analyzed for calcification rates and four replicates from SW and SW+100 treatments, for microarray analysis.
Techniques Used: Incubation, Sampling, Microarray
Related Articles
Incubation:Article Title: Dynamics of Membrane Potential Variation and Gene Expression Induced by Spodoptera littoralis , Myzus persicae , and Pseudomonas syringae in Arabidopsis Article Snippet: Then, 825 ng of control Cy3-RNAs and 825 ng of treated Cy5-RNAs were pooled together and hybridized using the Gene Expression Hybridization Kit (Agilent Technologies) onto 4×44 K Arabidopsis (v3) Oligo Microarray (Agilent Technologies), satisfying Minimum Information About a Microarray Experiment (MIAME) requirements . .. After a 17 h incubation at 65°C and 10 rpm, microarrays were first washed with Gene Expression Wash buffer 1 for 1 min, then with Gene Expression Wash buffer 2 for 1 min, then with 100% acetonitrile for 30 s, and finally washed in the Stabilization and Drying Solution for 30 s. Microarrays were scanned with the Agilent Microarray G2505B Scanner (with the extended dynamic range (XDR) scan mode to scan the same slide at two different levels and data were extracted and normalized from the resulting images using Agilent Feature Extraction (FE) software (v.9.5.1) Agilent Arabidopsis (v3) Gene Expression:Article Title: Dynamics of Membrane Potential Variation and Gene Expression Induced by Spodoptera littoralis , Myzus persicae , and Pseudomonas syringae in Arabidopsis Article Snippet: Then, 825 ng of control Cy3-RNAs and 825 ng of treated Cy5-RNAs were pooled together and hybridized using the Gene Expression Hybridization Kit (Agilent Technologies) onto 4×44 K Arabidopsis (v3) Oligo Microarray (Agilent Technologies), satisfying Minimum Information About a Microarray Experiment (MIAME) requirements . .. After a 17 h incubation at 65°C and 10 rpm, microarrays were first washed with Gene Expression Wash buffer 1 for 1 min, then with Gene Expression Wash buffer 2 for 1 min, then with 100% acetonitrile for 30 s, and finally washed in the Stabilization and Drying Solution for 30 s. Microarrays were scanned with the Agilent Microarray G2505B Scanner (with the extended dynamic range (XDR) scan mode to scan the same slide at two different levels and data were extracted and normalized from the resulting images using Agilent Feature Extraction (FE) software (v.9.5.1) Agilent Arabidopsis (v3) Article Title: Oct4 Gene Expression in Primary Colorectal Cancer Promotes Liver Metastasis Article Snippet: Oct4-EGFP-high cells and Oct4-EGFP-negative cells were sorted using an SH800 cell sorter (SONY), and total RNA was prepared using an RNA Purification Kit (Qiagen). .. A Article Title: Inflammatory priming with IL-1β promotes the immunomodulatory behavior of adipose derived stem cells Article Snippet: Agilent RNA ScreenTape System (Agilent Technologies, Santa Clara, CA, United States) was used to evaluate the RNA integrity ( ). .. A huge panel of genes of interest (maximum 3,000 genes, including at least five replicates for each gene) was selected and analyzed through a Article Title: Gene Expression Analysis of Alfalfa Seedlings Response to Acid-Aluminum Article Snippet: .. Global gene expression analysis using the Article Title: Integrative genetic and genomic networks identify microRNA associated with COPD and ILD Article Snippet: .. We utilized a subset of 262 lung tissue samples with miRNA expression profiled by sequencing, as well as an Article Title: Lymphatic endothelial sphingosine 1-phosphate receptor 1 enhances macrophage clearance via lymphatic system following myocardial infarction Article Snippet: Data were recorded with an LSR II flow cytometer (BD Biosciences) and analyzed with FlowJo (Version 9.0). .. Total RNA from hearts or LECs was extracted by Trizol (Invitrogen) following the manufacturer’s instructions and used for RT-PCR (the primer list was listed in ) or Article Title: Gene Expression Analysis of Alfalfa Seedlings Response to Acid-Aluminum Article Snippet: .. Twelve genes encoding peroxidase (GO:0004601) could eliminate ROS production, leading to the plant morphological changes induced by Al. A total of 20 genes encoding transporters were found in our Agilent Article Title: Commensal Microbiota Contributes to Chronic Endocarditis in TAX1BP1 Deficient Mice Article Snippet: .. Then, global mRNA expression profiles were analyzed by an Microarray:Article Title: Dynamics of Membrane Potential Variation and Gene Expression Induced by Spodoptera littoralis , Myzus persicae , and Pseudomonas syringae in Arabidopsis Article Snippet: Then, 825 ng of control Cy3-RNAs and 825 ng of treated Cy5-RNAs were pooled together and hybridized using the Gene Expression Hybridization Kit (Agilent Technologies) onto 4×44 K Arabidopsis (v3) Oligo Microarray (Agilent Technologies), satisfying Minimum Information About a Microarray Experiment (MIAME) requirements . .. After a 17 h incubation at 65°C and 10 rpm, microarrays were first washed with Gene Expression Wash buffer 1 for 1 min, then with Gene Expression Wash buffer 2 for 1 min, then with 100% acetonitrile for 30 s, and finally washed in the Stabilization and Drying Solution for 30 s. Microarrays were scanned with the Agilent Microarray G2505B Scanner (with the extended dynamic range (XDR) scan mode to scan the same slide at two different levels and data were extracted and normalized from the resulting images using Agilent Feature Extraction (FE) software (v.9.5.1) Agilent Arabidopsis (v3) Article Title: Oct4 Gene Expression in Primary Colorectal Cancer Promotes Liver Metastasis Article Snippet: Oct4-EGFP-high cells and Oct4-EGFP-negative cells were sorted using an SH800 cell sorter (SONY), and total RNA was prepared using an RNA Purification Kit (Qiagen). .. A Article Title: Inflammatory priming with IL-1β promotes the immunomodulatory behavior of adipose derived stem cells Article Snippet: Agilent RNA ScreenTape System (Agilent Technologies, Santa Clara, CA, United States) was used to evaluate the RNA integrity ( ). .. A huge panel of genes of interest (maximum 3,000 genes, including at least five replicates for each gene) was selected and analyzed through a Article Title: Gene Expression Analysis of Alfalfa Seedlings Response to Acid-Aluminum Article Snippet: .. Global gene expression analysis using the Article Title: Integrative genetic and genomic networks identify microRNA associated with COPD and ILD Article Snippet: .. We utilized a subset of 262 lung tissue samples with miRNA expression profiled by sequencing, as well as an Article Title: Lymphatic endothelial sphingosine 1-phosphate receptor 1 enhances macrophage clearance via lymphatic system following myocardial infarction Article Snippet: Data were recorded with an LSR II flow cytometer (BD Biosciences) and analyzed with FlowJo (Version 9.0). .. Total RNA from hearts or LECs was extracted by Trizol (Invitrogen) following the manufacturer’s instructions and used for RT-PCR (the primer list was listed in ) or Article Title: Gene Expression Analysis of Alfalfa Seedlings Response to Acid-Aluminum Article Snippet: .. Twelve genes encoding peroxidase (GO:0004601) could eliminate ROS production, leading to the plant morphological changes induced by Al. A total of 20 genes encoding transporters were found in our Agilent Article Title: Commensal Microbiota Contributes to Chronic Endocarditis in TAX1BP1 Deficient Mice Article Snippet: .. Then, global mRNA expression profiles were analyzed by an Extraction:Article Title: Dynamics of Membrane Potential Variation and Gene Expression Induced by Spodoptera littoralis , Myzus persicae , and Pseudomonas syringae in Arabidopsis Article Snippet: Then, 825 ng of control Cy3-RNAs and 825 ng of treated Cy5-RNAs were pooled together and hybridized using the Gene Expression Hybridization Kit (Agilent Technologies) onto 4×44 K Arabidopsis (v3) Oligo Microarray (Agilent Technologies), satisfying Minimum Information About a Microarray Experiment (MIAME) requirements . .. After a 17 h incubation at 65°C and 10 rpm, microarrays were first washed with Gene Expression Wash buffer 1 for 1 min, then with Gene Expression Wash buffer 2 for 1 min, then with 100% acetonitrile for 30 s, and finally washed in the Stabilization and Drying Solution for 30 s. Microarrays were scanned with the Agilent Microarray G2505B Scanner (with the extended dynamic range (XDR) scan mode to scan the same slide at two different levels and data were extracted and normalized from the resulting images using Agilent Feature Extraction (FE) software (v.9.5.1) Agilent Arabidopsis (v3) Software:Article Title: Dynamics of Membrane Potential Variation and Gene Expression Induced by Spodoptera littoralis , Myzus persicae , and Pseudomonas syringae in Arabidopsis Article Snippet: Then, 825 ng of control Cy3-RNAs and 825 ng of treated Cy5-RNAs were pooled together and hybridized using the Gene Expression Hybridization Kit (Agilent Technologies) onto 4×44 K Arabidopsis (v3) Oligo Microarray (Agilent Technologies), satisfying Minimum Information About a Microarray Experiment (MIAME) requirements . .. After a 17 h incubation at 65°C and 10 rpm, microarrays were first washed with Gene Expression Wash buffer 1 for 1 min, then with Gene Expression Wash buffer 2 for 1 min, then with 100% acetonitrile for 30 s, and finally washed in the Stabilization and Drying Solution for 30 s. Microarrays were scanned with the Agilent Microarray G2505B Scanner (with the extended dynamic range (XDR) scan mode to scan the same slide at two different levels and data were extracted and normalized from the resulting images using Agilent Feature Extraction (FE) software (v.9.5.1) Agilent Arabidopsis (v3) Expressing:Article Title: Dynamics of Membrane Potential Variation and Gene Expression Induced by Spodoptera littoralis , Myzus persicae , and Pseudomonas syringae in Arabidopsis Article Snippet: Then, 825 ng of control Cy3-RNAs and 825 ng of treated Cy5-RNAs were pooled together and hybridized using the Gene Expression Hybridization Kit (Agilent Technologies) onto 4×44 K Arabidopsis (v3) Oligo Microarray (Agilent Technologies), satisfying Minimum Information About a Microarray Experiment (MIAME) requirements . .. After a 17 h incubation at 65°C and 10 rpm, microarrays were first washed with Gene Expression Wash buffer 1 for 1 min, then with Gene Expression Wash buffer 2 for 1 min, then with 100% acetonitrile for 30 s, and finally washed in the Stabilization and Drying Solution for 30 s. Microarrays were scanned with the Agilent Microarray G2505B Scanner (with the extended dynamic range (XDR) scan mode to scan the same slide at two different levels and data were extracted and normalized from the resulting images using Agilent Feature Extraction (FE) software (v.9.5.1) Agilent Arabidopsis (v3) Article Title: Gene Expression Analysis of Alfalfa Seedlings Response to Acid-Aluminum Article Snippet: .. Global gene expression analysis using the Article Title: Integrative genetic and genomic networks identify microRNA associated with COPD and ILD Article Snippet: .. We utilized a subset of 262 lung tissue samples with miRNA expression profiled by sequencing, as well as an Article Title: Commensal Microbiota Contributes to Chronic Endocarditis in TAX1BP1 Deficient Mice Article Snippet: .. Then, global mRNA expression profiles were analyzed by an Construct:Article Title: Oct4 Gene Expression in Primary Colorectal Cancer Promotes Liver Metastasis Article Snippet: Oct4-EGFP-high cells and Oct4-EGFP-negative cells were sorted using an SH800 cell sorter (SONY), and total RNA was prepared using an RNA Purification Kit (Qiagen). .. A Sequencing:Article Title: Integrative genetic and genomic networks identify microRNA associated with COPD and ILD Article Snippet: .. We utilized a subset of 262 lung tissue samples with miRNA expression profiled by sequencing, as well as an Reverse Transcription Polymerase Chain Reaction:Article Title: Lymphatic endothelial sphingosine 1-phosphate receptor 1 enhances macrophage clearance via lymphatic system following myocardial infarction Article Snippet: Data were recorded with an LSR II flow cytometer (BD Biosciences) and analyzed with FlowJo (Version 9.0). .. Total RNA from hearts or LECs was extracted by Trizol (Invitrogen) following the manufacturer’s instructions and used for RT-PCR (the primer list was listed in ) or |
