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technical triplicates  (Sartorius AG)


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    Structured Review

    Sartorius AG technical triplicates
    Technical Triplicates, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 99/100, based on 16124 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/triplicate/Live+Cell+Analysis+Instruments/pm42844286-365-16-18
    Average 99 stars, based on 16124 article reviews
    technical triplicates - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Cell Characterization:

    Article Title: Targeting YAP/TEAD signaling disturbs RNA Pol II activity and enhances immunotherapy response via activated cytosolic DNA sensing pathway in gastroesophageal cancer
    Article Snippet: Cells were cultured in RPMI1640 (Invitrogen) or DMEM (Invitrogen) and supplemented with 10% fetal bovine serum (FBS) (Invitrogen), 100 U/ml penicillin, and 100 mg/ml streptomycin (Invitrogen), and maintained at 37°C in a humidified incubator of 5% CO2. .. Live-Cell proliferation was measured using the IncuCyte cell count proliferation assay (Sartorius). ..

    Proliferation Assay:

    Article Title: Targeting YAP/TEAD signaling disturbs RNA Pol II activity and enhances immunotherapy response via activated cytosolic DNA sensing pathway in gastroesophageal cancer
    Article Snippet: Cells were cultured in RPMI1640 (Invitrogen) or DMEM (Invitrogen) and supplemented with 10% fetal bovine serum (FBS) (Invitrogen), 100 U/ml penicillin, and 100 mg/ml streptomycin (Invitrogen), and maintained at 37°C in a humidified incubator of 5% CO2. .. Live-Cell proliferation was measured using the IncuCyte cell count proliferation assay (Sartorius). ..

    Software:

    Article Title: Species-specific inflammatory responses of chondrocytes: Toward evidence-based model selection in OA research
    Article Snippet: .. IncuCyte software analysis , Sartorius , RRID:SCR_003935; https://www.sartorius.com/en/product. ..

    Article Title: Targeting CD28 on T lineage malignancies with chimeric antigen receptor T cells.
    Article Snippet: .. Quantification of cellular abundance was performed as recommended by measuring GFP integrated intensity in at least technical triplicates (Sartorius, Incucyte Software Version 2023A-2025B). ..

    Article Title: Astrocytes regulate axonal mitochondrial transport deficits in C9ORF72 amyotrophic lateral sclerosis motor neurons.
    Article Snippet: .. Software and code Policy information about availability of computer code Data collection Zeiss Zen software (Carl Zeiss), Seahorse Wave Desktop Software v2.6 (Agilent Technologies), Harmony software v4.9 (PerkinElmer), IncuCyte software (Sartorius), and ImageJ/Fiji v2.14. .. Data analysis GraphPad Prism v10, R v4.3.2 (including lme4, car, emmeans and DESeq2 packages), and ImageJ/Fiji v2.14 For manuscripts utilizing custom algorithms or software that are central to the research but not yet described in published literature, software must be made available to editors and reviewers.

    Analysis:

    Article Title: Species-specific inflammatory responses of chondrocytes: Toward evidence-based model selection in OA research
    Article Snippet: .. IncuCyte software analysis , Sartorius , RRID:SCR_003935; https://www.sartorius.com/en/product. ..

    Article Title: Differential effects of propranolol enantiomers on haemangioma-derived endothelial spheroids
    Article Snippet: The spheroids were embedded in Cultrex Reduced Growth Factor Basement Membrane Extract (R&D Systems, NE Minneapolis, MN, USA) at a concentration of 4–5 mg/mL, following removal of growth medium and incubated at 37 °C to allow polymerisation. .. Culture media was added on top and whole spheroid area and the sprouting area was monitored over 3 days with the Incucyte Live-Cell Analysis (Sartorius, Essen Bio, Ann Arbor, MI, USA) with scans performed every 24 h using a 4 × objective. ..

    Article Title: Cold-Atmospheric-Plasma-Treated MSCs Retain Stem Cell Phenotype and Trilineage Differentiation Potential
    Article Snippet: .. Changes in hTERT-MSC fibroblast-like morphology were investigated following each treatment for the duration of the incubation (24, 48, 72 h) using the Incucyte Live-Cell https://doi.org/10.3390/plasma9040043 Analysis Instrument (Sartorius, Göttingen, Germany) in phase-contrast mode with the S3/SX 1 G/R Optical Module. .. To determine the MSC phenotype of hTERT-MSCs, the expression of positive markers CD105, CD90 and CD73 and the negative marker HLA/DR was analysed by flow cytometry using an MSC Phenotyping Cocktail Kit, anti-human, REAfinity (Miltenyi Biotec, Bisley, UK).

    Staining:

    Article Title: Gasdermin D-mediated delivery of caspase inhibitors to suppress pyroptosis.
    Article Snippet: Caspase-1, -4, -5 and -11 activate gasdermin D (GSDMD) pores, causing pyroptotic cell death and the release of the interleukins IL-1β and IL-18 (ref. 1).. Blocking this pathway holds therapeutic promise for the treatment of inflammatory disorders, but cellpermeable caspase inhibitors have not proved successful in clinical trials.. Here we describe covalent caspase inhibitors that selectively block pyroptosis and IL-1β secretion despite being excluded from healthy cells.

    Morphology:

    Article Title: Cold-Atmospheric-Plasma-Treated MSCs Retain Stem Cell Phenotype and Trilineage Differentiation Potential
    Article Snippet: .. Changes in hTERT-MSC fibroblast-like morphology were investigated following each treatment for the duration of the incubation (24, 48, 72 h) using the Incucyte Live-Cell https://doi.org/10.3390/plasma9040043 Analysis Instrument (Sartorius, Göttingen, Germany) in phase-contrast mode with the S3/SX 1 G/R Optical Module. .. To determine the MSC phenotype of hTERT-MSCs, the expression of positive markers CD105, CD90 and CD73 and the negative marker HLA/DR was analysed by flow cytometry using an MSC Phenotyping Cocktail Kit, anti-human, REAfinity (Miltenyi Biotec, Bisley, UK).

    Incubation:

    Article Title: Cold-Atmospheric-Plasma-Treated MSCs Retain Stem Cell Phenotype and Trilineage Differentiation Potential
    Article Snippet: .. Changes in hTERT-MSC fibroblast-like morphology were investigated following each treatment for the duration of the incubation (24, 48, 72 h) using the Incucyte Live-Cell https://doi.org/10.3390/plasma9040043 Analysis Instrument (Sartorius, Göttingen, Germany) in phase-contrast mode with the S3/SX 1 G/R Optical Module. .. To determine the MSC phenotype of hTERT-MSCs, the expression of positive markers CD105, CD90 and CD73 and the negative marker HLA/DR was analysed by flow cytometry using an MSC Phenotyping Cocktail Kit, anti-human, REAfinity (Miltenyi Biotec, Bisley, UK).

    Phase Contrast:

    Article Title: Cold-Atmospheric-Plasma-Treated MSCs Retain Stem Cell Phenotype and Trilineage Differentiation Potential
    Article Snippet: .. Changes in hTERT-MSC fibroblast-like morphology were investigated following each treatment for the duration of the incubation (24, 48, 72 h) using the Incucyte Live-Cell https://doi.org/10.3390/plasma9040043 Analysis Instrument (Sartorius, Göttingen, Germany) in phase-contrast mode with the S3/SX 1 G/R Optical Module. .. To determine the MSC phenotype of hTERT-MSCs, the expression of positive markers CD105, CD90 and CD73 and the negative marker HLA/DR was analysed by flow cytometry using an MSC Phenotyping Cocktail Kit, anti-human, REAfinity (Miltenyi Biotec, Bisley, UK).



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