trap solution substrate (Millipore)

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Millipore trap solution substrate

(A) Wild-type or TRAF6-deficient bone marrow macrophages (BMM) retrovirally-rescued with wild-type (WT), RING mutant (C70A), or lysine-deficient (ΔK) full-length versions of FLAG-TRAF6 were treated as indicated with RANKL, then lysed and subjected to immunoblotting against the activated phosphorylated forms of IκBα, JNK, and p38. B , BMM described in (A) were replated and cultured with M-CSF and RANKL for 5 days to induce osteoclast differentiation. (C) Osteoclasts depicted in (B) were fixed and subjected to <t>TRAP</t> <t>solution</t> assay and quantified at 405 nm absorbance. (D) Total cell counts per well of retrovirally-rescued osteoclasts depicted in (B) as defined by cells containing at least 3 nuclei and being at least 100 µM in diameter.

Trap Solution Substrate, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/trap+substrate+solution/trap+solution/pmc02603309-133-0-9
Average 90 stars, based on 1 article reviews

trap solution substrate - by Bioz Stars, 2026-09

90/100 stars

Images

1) Product Images from "TRAF6 Autoubiquitination-Independent Activation of the NFκB and MAPK Pathways in Response to IL-1 and RANKL"

Article Title: TRAF6 Autoubiquitination-Independent Activation of the NFκB and MAPK Pathways in Response to IL-1 and RANKL

Journal: PLoS ONE

doi: 10.1371/journal.pone.0004064

Figure Legend Snippet: (A) Wild-type or TRAF6-deficient bone marrow macrophages (BMM) retrovirally-rescued with wild-type (WT), RING mutant (C70A), or lysine-deficient (ΔK) full-length versions of FLAG-TRAF6 were treated as indicated with RANKL, then lysed and subjected to immunoblotting against the activated phosphorylated forms of IκBα, JNK, and p38. B , BMM described in (A) were replated and cultured with M-CSF and RANKL for 5 days to induce osteoclast differentiation. (C) Osteoclasts depicted in (B) were fixed and subjected to TRAP solution assay and quantified at 405 nm absorbance. (D) Total cell counts per well of retrovirally-rescued osteoclasts depicted in (B) as defined by cells containing at least 3 nuclei and being at least 100 µM in diameter.

Techniques Used: Mutagenesis, Western Blot, Cell Culture

Incubation:

Article Title: The mechanism of pyrroloquinoline quinone influencing the fracture healing process of estrogen-deficient mice by inhibiting oxidative stress.
Article Snippet: .. Then preincubated the tissue with 50 mM sodium acetate and 40 mM potassium sodium tartrate buffer (PH5.0) for 20 min, incubated with TRAP substrate solution containing 25 mg naphthol AS-MX phosphate (N4875, Sigma-Aldrich, St. Louis, MO, USA) dissolved in 1 mL dimethylformamide and 5 mg Fast Garnet GBC (F8761, Sigma-Aldrich, St. Louis, MO, USA) dissolved in 50 mM sodium acetate and 40 mM potassium sodium tartrate buffer) for 15 min at room temperature, wash with distilled water, counterstaining with Vector methyl green (H-3402, Vector, USA) nuclear counterstain and mounted with glycerol jelly. .. The cells from fractured bone were converted into single cell suspensions with syringe, then washed with cold PBS, and resuspended in binding buffer, then 106 cells per sample were incubated with 5 μL 2′,7′- dichlorofluorescein diacetate (DCFH-DA, Sigma-Aldrich, St. Louis, MO, USA) for 30 mins in the dark followed by incubation with 10% FBS for 20 mins at 37 ◦C.

Article Title: Mutation of PFN1 Gene in an Early Onset, Polyostotic Paget-like Disease.
Article Snippet: .. Tartrate-resistant acid phosphatase (TRAP) staining was performed after 7 to 8 days (murine) or 14 to 15 days (human) of differentiation, after which they were fixed for 10 minutes in 4% paraformaldehyde, washed in distilled water, and incubated with TRAP solution (387A acid phosphatase, Leukocyte TRAP Kit, SIGMA) for 15 to 30 minutes at 37°C and consequently washed. ..

In Situ:

Article Title: Histone Lys demethylase KDM3C demonstrates anti-inflammatory effects by suppressing NF-κB signaling and osteoclastogenesis
Article Snippet: .. To detect osteoclasts in Kdm3c WT and KO mice in situ , the histologic sections were stained with the TRAP solution (MilliporeSigma). .. Quantitative measurement of the TRAP staining was performed on digital pictures taken through Olympus microscope (Olympus, Tokyo, Japan) at ×100 magnification by counting the TRAP + multinucleated cells with more than 5 nuclei ( 14 ).

Article Title: Histone Lys demethylase KDM3C demonstrates anti‐inflammatory effects by suppressing NF‐κB signaling and osteoclastogenesis
Article Snippet: .. To detect osteoclasts in Kdm3c WT and KO mice in situ, the histologic sections were stained with the TRAP solution (MilliporeSigma). .. Quantitative measurement of the TRAP staining was performed on digital pictures taken through Olympus microscope (Olympus, Tokyo, Japan) at 3100 magnification by counting the TRAP+ multinucleated cells with more than 5 nuclei (14).

Staining:

Article Title: Synergistic effects of miR-708-5p and miR-708-3p accelerate the progression of osteoporosis.
Article Snippet: .. Briefly, the multinucleated osteoclasts were fixed with 3.7% formalin for 10 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and then stained with TRAP solution (SigmaAldrich, Saint Louis, MO, USA). ..

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