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elf97 staining solution  (Thermo Fisher)


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    Structured Review

    Thermo Fisher elf97 staining solution
    Elf97 Staining Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/trap+substrate+solution/Phosphate/pm41980979-221-43-49
    Average 99 stars, based on 1 article reviews
    elf97 staining solution - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Saline:

    Article Title: Revolutionizing Mg-based guided bone regeneration mesh derived from endogenous dentoalveolar bone augmentation
    Article Snippet: .. The cells were rinsed twice with DPBS (Dulbecco's Phosphate-Buffered Saline), then TRIzol reagent (Thermo Fisher Scientific, USA) was added. .. RNA extraction, transcriptome sequencing, and subsequent analysis were performed by Shanghai OE Biotech Co., Ltd. RNA purity and quantification were assessed using a NanoDrop 2000 (Thermo Scientific, USA).

    Article Title: From biowastes to risks? Impact of biosolids treatment and dose on antibiotic resistance in agricultural soils – A mesocosm study
    Article Snippet: .. Biosolid (2.5 g) and soil (5 g) samples were suspended in 40 ml phosphate-buffered saline (PBS, pH 7.2) with glass beads (4 mm Ø, Marienfeld, Germany) and vortexed for 2 min. After settling for 5 min, 5 ml of supernatant was serially diluted (10 −6 to 10 −1 ) with Phosphate Buffer Saline (PBS), and 100 μl aliquots were spread on Reasoner's 2 A (R2A) agar plates (OXOID, USA) for the determination of total heterotrophic bacteria. ..

    Article Title: Near infrared enhanced palladium loaded siraitia grosvenorii carbon dots amplify mitophagy for acute lung injury immunotherapy
    Article Snippet: .. In brief, CDs, CPs and CPs@SS31 were initially dispersed in different solutions: phosphate buffer saline (PBS, pH = 7.6, Gibco, USA), Dulbecco's modified eagle medium (DMEM, Gibco, USA), fetal bovine serum (FBS, Gibco, Billings, USA) and 5 mM H 2 O 2 (Aladdin, China) respectively. ..

    Bacteria:

    Article Title: From biowastes to risks? Impact of biosolids treatment and dose on antibiotic resistance in agricultural soils – A mesocosm study
    Article Snippet: .. Biosolid (2.5 g) and soil (5 g) samples were suspended in 40 ml phosphate-buffered saline (PBS, pH 7.2) with glass beads (4 mm Ø, Marienfeld, Germany) and vortexed for 2 min. After settling for 5 min, 5 ml of supernatant was serially diluted (10 −6 to 10 −1 ) with Phosphate Buffer Saline (PBS), and 100 μl aliquots were spread on Reasoner's 2 A (R2A) agar plates (OXOID, USA) for the determination of total heterotrophic bacteria. ..

    Expressing:

    Article Title: Mitochondrial inhibition enhances the sensitivity of pancreatic ductal adenocarcinoma cells to oncolytic adenovirus
    Article Snippet: Total RNA was extracted from cells at 2, 24, and 48 h after infection with OBP-301 or OBP-702 at an MOI of 10 PFU/cell using the miRNeasy Mini Kit (Qiagen). cDNA was synthesized from 10 ng of total RNA using a TaqMan Reverse Transcription Kit (Applied Biosystems). .. The expression levels of hTERT, p53, and glyceride-hyde-3-phosphate dehydrogenase (GAPDH) mRNA were determined using quantitative real-time PCR on an Applied Biosystems StepOnePlus real-time PCR system (Applied Biosystems). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Mitochondrial inhibition enhances the sensitivity of pancreatic ductal adenocarcinoma cells to oncolytic adenovirus
    Article Snippet: Total RNA was extracted from cells at 2, 24, and 48 h after infection with OBP-301 or OBP-702 at an MOI of 10 PFU/cell using the miRNeasy Mini Kit (Qiagen). cDNA was synthesized from 10 ng of total RNA using a TaqMan Reverse Transcription Kit (Applied Biosystems). .. The expression levels of hTERT, p53, and glyceride-hyde-3-phosphate dehydrogenase (GAPDH) mRNA were determined using quantitative real-time PCR on an Applied Biosystems StepOnePlus real-time PCR system (Applied Biosystems). ..

    Article Title: Effects of dietary saponin and polyphenol supplementation in broiler chickens exposed to multiple mild stressors of cyclic elevated ambient temperature, feed withdrawal, and coccidiosis infection
    Article Snippet: Gene details and National Center for Biotechnology Information reference numbers are shown in . .. Amplification by real-time, quantitative PCR was achieved for target (HSP70, HSP90, IL-1β, IL-10, OCLN, ZO-1) and reference (glyceraldehyde-3-phosphate dehydrogenase; GAPDH ; NM_204305.1 ; Thermo Fisher Inc.) genes. .. Synthesized cDNA was amplified with Taqman (Cat No.: 4304437; Thermo Fisher Scientific Inc) oligonucleotide probes containing 5′ fluorescent reporter dye and 3′ non-fluorescent quencher dye.

    Amplification:

    Article Title: Effects of dietary saponin and polyphenol supplementation in broiler chickens exposed to multiple mild stressors of cyclic elevated ambient temperature, feed withdrawal, and coccidiosis infection
    Article Snippet: Gene details and National Center for Biotechnology Information reference numbers are shown in . .. Amplification by real-time, quantitative PCR was achieved for target (HSP70, HSP90, IL-1β, IL-10, OCLN, ZO-1) and reference (glyceraldehyde-3-phosphate dehydrogenase; GAPDH ; NM_204305.1 ; Thermo Fisher Inc.) genes. .. Synthesized cDNA was amplified with Taqman (Cat No.: 4304437; Thermo Fisher Scientific Inc) oligonucleotide probes containing 5′ fluorescent reporter dye and 3′ non-fluorescent quencher dye.

    Modification:

    Article Title: Near infrared enhanced palladium loaded siraitia grosvenorii carbon dots amplify mitophagy for acute lung injury immunotherapy
    Article Snippet: .. In brief, CDs, CPs and CPs@SS31 were initially dispersed in different solutions: phosphate buffer saline (PBS, pH = 7.6, Gibco, USA), Dulbecco's modified eagle medium (DMEM, Gibco, USA), fetal bovine serum (FBS, Gibco, Billings, USA) and 5 mM H 2 O 2 (Aladdin, China) respectively. ..

    Concentration Assay:

    Article Title: RNA detection on a microfluidic platform using Thyclotides.
    Article Snippet: .. The precipitate was diluted with phosphate buffer (500 μl, 5 mM), and finally, the concentration of the thyclotidegold nanoparticle conjugates was determined by a Nanodrop spectrophotometer. ..

    Spectrophotometry:

    Article Title: RNA detection on a microfluidic platform using Thyclotides.
    Article Snippet: .. The precipitate was diluted with phosphate buffer (500 μl, 5 mM), and finally, the concentration of the thyclotidegold nanoparticle conjugates was determined by a Nanodrop spectrophotometer. ..

    other:

    Article Title: A method for reconstituting the motility of membrane-bound myosin on the surface of the cell-sized W/O droplet
    Article Snippet: Proteins: • 2.7 μM myosin ID (stored at −80 °C) • 123 μM calmodulin (stored at −80 °C) • 60 μM G-actin (stored in liquid nitrogen) Chemicals and Reagents: • 1 M Sucrose (#30404–45, Nacalai Tesque, Japan) • 1 M Glucose (#049–31165, Fujifilm Wako Pure Chemical, Japan) • 100 mM ATP (#A7699, Sigma-Aldrich, USA) • 132 μM Alexa488-phalloidin (#A12379, Thermo-Fisher, USA) • ATP regeneration system (500 mM creatine phosphate (#10621714001, Roche, Switzerland) and 30 mg mL −1 creatine phosphokinase (#10127566001, Roche, Switzerland)) • Oxygen scavenging system (5 kU mL −1 glucose oxidase (#G2133, Sigma-Aldrich, USA) and 4 mg mL -1 catalase (#C3155, Sigma-Aldrich, USA)) Buffer: • AT buffer (50 mM Tris–HCl, pH 7.4, 25 mM KCl, 1 mM MgCl 2 , and 1 mM EGTA) Step-by-step protocol: 1.



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    (A) Wild-type or TRAF6-deficient bone marrow macrophages (BMM) retrovirally-rescued with wild-type (WT), RING mutant (C70A), or lysine-deficient (ΔK) full-length versions of FLAG-TRAF6 were treated as indicated with RANKL, then lysed and subjected to immunoblotting against the activated phosphorylated forms of IκBα, JNK, and p38. B , BMM described in (A) were replated and cultured with M-CSF and RANKL for 5 days to induce osteoclast differentiation. (C) Osteoclasts depicted in (B) were fixed and subjected to TRAP solution assay and quantified at 405 nm absorbance. (D) Total cell counts per well of retrovirally-rescued osteoclasts depicted in (B) as defined by cells containing at least 3 nuclei and being at least 100 µM in diameter.

    Journal: PLoS ONE

    Article Title: TRAF6 Autoubiquitination-Independent Activation of the NFκB and MAPK Pathways in Response to IL-1 and RANKL

    doi: 10.1371/journal.pone.0004064

    Figure Lengend Snippet: (A) Wild-type or TRAF6-deficient bone marrow macrophages (BMM) retrovirally-rescued with wild-type (WT), RING mutant (C70A), or lysine-deficient (ΔK) full-length versions of FLAG-TRAF6 were treated as indicated with RANKL, then lysed and subjected to immunoblotting against the activated phosphorylated forms of IκBα, JNK, and p38. B , BMM described in (A) were replated and cultured with M-CSF and RANKL for 5 days to induce osteoclast differentiation. (C) Osteoclasts depicted in (B) were fixed and subjected to TRAP solution assay and quantified at 405 nm absorbance. (D) Total cell counts per well of retrovirally-rescued osteoclasts depicted in (B) as defined by cells containing at least 3 nuclei and being at least 100 µM in diameter.

    Article Snippet: TRAP solution substrate and Coumermycin A were purchased from Sigma (St. Louis, MO).

    Techniques: Mutagenesis, Western Blot, Cell Culture