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tartrate-resistant acid phosphatase (trap) substrate solution mixed with tartrate  (Cosmo Bio USA)

 
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    Cosmo Bio USA tartrate-resistant acid phosphatase (trap) substrate solution mixed with tartrate
    Tartrate Resistant Acid Phosphatase (Trap) Substrate Solution Mixed With Tartrate, supplied by Cosmo Bio USA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/trap+substrate+solution/tartrate+resistant+acid+phosphatase++trap++staining+kit/pm38361265-175-8-19
    Average 90 stars, based on 1 article reviews
    tartrate-resistant acid phosphatase (trap) substrate solution mixed with tartrate - by Bioz Stars, 2026-09
    90/100 stars

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    other:

    Article Title: Genome Mining of a Deep-Sea-Derived Penicillium allii-sativi Revealed Polyketide-Terpenoid Hybrids with Antiosteoporosis Activity.
    Article Snippet: Following fixation, the cells were stained for tartrate- resistant acid phosphatase (TRAP) activity (Cosmo Bio LTD, PMC-AK04-COS).

    Article Title: Protocols for collecting mouse PDL cells and bone marrow cells, differentiation, and data analysis
    Article Snippet: Tartrate-resistant acid phosphatase (TRAP) staining kit , Cosmo Bio , Cat# PMC-AK04F-COS.

    Article Title: Secretory leucocyte protease inhibitor regulates bone metabolism and inflammation in experimental mouse periodontitis.
    Article Snippet: The prepared coronal sections (10 μm) were stained with tartrate-resistant acid phosphatase (TRAP) staining (Cosmo Bio Co., Tokyo, Japan).

    Article Title: Allyl isothiocyanate suppressed periodontal tissue destruction in mice via bacteriostatic and anti-inflammatory activities against Porphyromonas gingivalis.
    Article Snippet: Objectives: Allyl isothiocyanate (AITC) is a phytochemical that is abundantly present in cruciferous vegetables, such as wasabi and mustard.. Among its pharmacological properties, it demonstrates anticancer, antifungal, and anti-inflammatory activities.. This study aimed to investigate the functions of AITC against periodontopathic bacteria and its effects on a mouse model of periodontitis.

    Article Title: Conditioned Medium From Stem Cells of Human Exfoliated Deciduous Teeth Alleviates Mouse Osteoarthritis by Inducing sFRP1-Expressing M2 Macrophages.
    Article Snippet: Sagittal paraffin sections were generated and randomly selected for hematoxylin and eosin (HE; Sakura Finetek Japan, Tokyo, Japan), toluidine blue (TB; Fujifilm Wako), and tartrate-resistant acid phosphatase (TRAP; Cosmo Bio Co., LTD., Tokyo, Japan) with methyl green solution (Fujifilm Wako) counterstaining.

    Article Title: PDE4 Inhibition Reduced Osteoclast Differentiation in Psoriatic Patients.
    Article Snippet: Osteoclast precursors (OCPs) were identified by staining for tartrate-resistant acid phosphatase (TRAP; Cosmo Bio, Carlsbad, CA, USA; Cat.No.PMC-AK04F-COS), following the manufacturer’s instructions.

    Article Title: Hypoxia enhances osteoclastogenesis in periodontal ligament cells via expression of VEGF and RANKL
    Article Snippet: Introduction: Periodontal ligament (PDL) damage caused by dental trauma can lead to local circulatory disorders.. The mechanisms through which PDL cells, once exposed to a transient hypoxic environment, contribute to tissue regeneration or resorption of pathological tooth roots after reoxygenation remain unclear.. Therefore, we aimed to examine how changes in oxygen (O2) concentration affect PDL healing.

    Staining:

    Article Title: Targeting osteoclast-derived DPP4 alleviates inflammation-mediated ectopic bone formation in ankylosing spondylitis.
    Article Snippet: .. At the end of the culture period, mature osteoclasts were fixed in 10% formalin and stained for acid phosphatase 5, tartrate resistant (TRAP) using a commercial kit (PMC-AK04-COS; CosmoBio) according to the manufacturer’s protocol. ..



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    (A) Wild-type or TRAF6-deficient bone marrow macrophages (BMM) retrovirally-rescued with wild-type (WT), RING mutant (C70A), or lysine-deficient (ΔK) full-length versions of FLAG-TRAF6 were treated as indicated with RANKL, then lysed and subjected to immunoblotting against the activated phosphorylated forms of IκBα, JNK, and p38. B , BMM described in (A) were replated and cultured with M-CSF and RANKL for 5 days to induce osteoclast differentiation. (C) Osteoclasts depicted in (B) were fixed and subjected to TRAP solution assay and quantified at 405 nm absorbance. (D) Total cell counts per well of retrovirally-rescued osteoclasts depicted in (B) as defined by cells containing at least 3 nuclei and being at least 100 µM in diameter.

    Journal: PLoS ONE

    Article Title: TRAF6 Autoubiquitination-Independent Activation of the NFκB and MAPK Pathways in Response to IL-1 and RANKL

    doi: 10.1371/journal.pone.0004064

    Figure Lengend Snippet: (A) Wild-type or TRAF6-deficient bone marrow macrophages (BMM) retrovirally-rescued with wild-type (WT), RING mutant (C70A), or lysine-deficient (ΔK) full-length versions of FLAG-TRAF6 were treated as indicated with RANKL, then lysed and subjected to immunoblotting against the activated phosphorylated forms of IκBα, JNK, and p38. B , BMM described in (A) were replated and cultured with M-CSF and RANKL for 5 days to induce osteoclast differentiation. (C) Osteoclasts depicted in (B) were fixed and subjected to TRAP solution assay and quantified at 405 nm absorbance. (D) Total cell counts per well of retrovirally-rescued osteoclasts depicted in (B) as defined by cells containing at least 3 nuclei and being at least 100 µM in diameter.

    Article Snippet: TRAP solution substrate and Coumermycin A were purchased from Sigma (St. Louis, MO).

    Techniques: Mutagenesis, Western Blot, Cell Culture