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trap staining solution g1050  (Servicebio Inc)

 
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    Servicebio Inc trap staining solution g1050
    Trap Staining Solution G1050, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/trap+staining+solution/kit+staining+trap/pm41006700-274-12-19
    Average 86 stars, based on 1 article reviews
    trap staining solution g1050 - by Bioz Stars, 2026-10
    86/100 stars

    Images

    Related Articles

    Staining:

    Article Title: Targeting the PARP10-BCAT2 axis disrupts branched-chain amino-acid metabolism to suppress bone metastasis in lung cancer.
    Article Snippet: .. TRAP staining of osteoclasts in decalcified bone sections was performed by Wuhan Servicebio Technology Co., Ltd. (Wuhan, Hubei, China) following a standard automated histology protocol. ..

    Article Title: SMN deficiency contributes to osteoporosis in spinal muscular atrophy by impairing Snap23 meditated muscle-derived extracellular vesicle secretion.
    Article Snippet: .. Sections were stained with hematoxylin and eosin (H&E) staining kit (#G1005, Servicebio, China). ..

    Article Title: SMN deficiency contributes to osteoporosis in spinal muscular atrophy by impairing Snap23 meditated muscle-derived extracellular vesicle secretion.
    Article Snippet: .. After paraffin embedding and sectioning (5 μm), sections were stained with H&E (#G1005, Servicebio, China) or for tartrate-resistant acid phosphatase (TRAP) activity using a TRAP staining kit (#G1050, Servicebio, China). .. For immunofluorescence AR TIC LE IN PR ES S staining, sections were incubated with a rabbit monoclonal anti-COL1 antibody (1:200, #ab270993, Abcam) overnight at 4°C, followed by incubation with a goat anti-rabbit Alexa Fluor 555 secondary antibody (1:500, #A21428, Invitrogen) for 30 minutes at 37°C.

    Article Title: SMN deficiency contributes to osteoporosis in spinal muscular atrophy by impairing Snap23 meditated muscle-derived extracellular vesicle secretion.
    Article Snippet: Nuclei were counterstained with DAPI (#D8200, Solarbio) for 10 minutes. .. Cells: Cells were fixed with 4% paraformaldehyde for 10 minutes and stained for TRAP activity (#G1050, Servicebio, China) according to the manufacturer's protocol. .. All stained sections and cells were imaged using a confocal laser scanning microscope (Olympus, Japan) or a light microscope.

    Article Title: A New Wnt1 Mutant Rat Model of Osteogenesis Imperfecta and Its Application in AAV9‐Mediated Gene Therapy
    Article Snippet: .. Sections were stained with hematoxylin and eosin (H&E), Masson′s trichrome, and a tartrate‐resistant Acid phosphatase (TRAP) commercial staining kit (TRAP Staining Kit, Servicebio, China). .. Stained sections were visualized with an optical microscope (NIKON ECLIPSE E100, Nikon, Japan).

    Article Title: Phenylethanoid Glycosides from Cistanche tubulosa (Schenk) Wight Improve Radiation-Induced Bone Injury and Reduce Grem1 Expression in Osteoblasts.
    Article Snippet: .. Freshly prepared TRAP incubation solution (Servicebio, G1050, Wuhan, China) was added, and sections were incubated at 37 ◦C for 20 min. After staining, nuclei were counterstained with hematoxylin for approximately 15 s, differentiated, and blued (sections were immersed into the reverse blue solution for 10 s, then removed, and washed with water). ..

    Activity Assay:

    Article Title: SMN deficiency contributes to osteoporosis in spinal muscular atrophy by impairing Snap23 meditated muscle-derived extracellular vesicle secretion.
    Article Snippet: .. After paraffin embedding and sectioning (5 μm), sections were stained with H&E (#G1005, Servicebio, China) or for tartrate-resistant acid phosphatase (TRAP) activity using a TRAP staining kit (#G1050, Servicebio, China). .. For immunofluorescence AR TIC LE IN PR ES S staining, sections were incubated with a rabbit monoclonal anti-COL1 antibody (1:200, #ab270993, Abcam) overnight at 4°C, followed by incubation with a goat anti-rabbit Alexa Fluor 555 secondary antibody (1:500, #A21428, Invitrogen) for 30 minutes at 37°C.

    Article Title: SMN deficiency contributes to osteoporosis in spinal muscular atrophy by impairing Snap23 meditated muscle-derived extracellular vesicle secretion.
    Article Snippet: Nuclei were counterstained with DAPI (#D8200, Solarbio) for 10 minutes. .. Cells: Cells were fixed with 4% paraformaldehyde for 10 minutes and stained for TRAP activity (#G1050, Servicebio, China) according to the manufacturer's protocol. .. All stained sections and cells were imaged using a confocal laser scanning microscope (Olympus, Japan) or a light microscope.

    other:

    Article Title: Amuc_1473 Links Gut Microbes to Skeletal Homeostasis and Counteracts Multifactorial Osteoporosis.
    Article Snippet: RAW264.7 cells (ATCC; ockville, USA) were cultured in high-glucose Dulbecco’s modfied Eagle’s medium (DMEM; Gibco) with 10% FBS and 1% PS.

    Incubation:

    Article Title: Phenylethanoid Glycosides from Cistanche tubulosa (Schenk) Wight Improve Radiation-Induced Bone Injury and Reduce Grem1 Expression in Osteoblasts.
    Article Snippet: .. Freshly prepared TRAP incubation solution (Servicebio, G1050, Wuhan, China) was added, and sections were incubated at 37 ◦C for 20 min. After staining, nuclei were counterstained with hematoxylin for approximately 15 s, differentiated, and blued (sections were immersed into the reverse blue solution for 10 s, then removed, and washed with water). ..



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    trap staining solution  (Servicebio Inc)
    86
    Servicebio Inc trap staining solution
    Effect of NGF-G/P@Yoda1 on promoting bone regeneration and bone remodeling in femur def ect model. (A–D) Representative <t>immunohistochemical</t> <t>staining</t> images at week 8 for RUNX2 and OPN and semi-quantitative analysis of positive areas to evaluate the osteogenic capacity of composite hydrogels. (E, F) Representative immunofluorescence (IF) staining images at week 8 of OCN and semi-quantitative analysis. (G–J) Representative IF staining images at week 8 of YAP1and β-catenin and semi-quantitative analyses. (K, L) Representative <t>TRAP</t> staining images at week 8 and semi-quantitative analysis of positive areas to assess bone resorption and remodeling. The quantitative data (n ≥ 3) in the figures represent as the averages ± SD, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.
    Trap Staining Solution, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/trap+staining+solution/kit+staining+trap/pmc12670961-217-11-15
    Average 86 stars, based on 1 article reviews
    trap staining solution - by Bioz Stars, 2026-10
    86/100 stars
    		  Buy from Supplier
    trap staining solution g1050  (Servicebio Inc)
    86
    Servicebio Inc trap staining solution g1050
    Effect of NGF-G/P@Yoda1 on promoting bone regeneration and bone remodeling in femur def ect model. (A–D) Representative <t>immunohistochemical</t> <t>staining</t> images at week 8 for RUNX2 and OPN and semi-quantitative analysis of positive areas to evaluate the osteogenic capacity of composite hydrogels. (E, F) Representative immunofluorescence (IF) staining images at week 8 of OCN and semi-quantitative analysis. (G–J) Representative IF staining images at week 8 of YAP1and β-catenin and semi-quantitative analyses. (K, L) Representative <t>TRAP</t> staining images at week 8 and semi-quantitative analysis of positive areas to assess bone resorption and remodeling. The quantitative data (n ≥ 3) in the figures represent as the averages ± SD, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.
    Trap Staining Solution G1050, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/trap+staining+solution/kit+staining+trap/pm41006700-274-12-19
    Average 86 stars, based on 1 article reviews
    trap staining solution g1050 - by Bioz Stars, 2026-10
    86/100 stars
    		  Buy from Supplier
    trap staining solution  (Beyotime)
    99
    Beyotime trap staining solution
    pBTO inhibited osteoclastogenesis in vitro. (A) <t>TRAP</t> <t>staining</t> in RANKL-treated RAW264.7 cells stimulated with BTO and pBTO. (B) Relative mRNA levels of genes associated with osteoclastogenesis of RAW264.7 cells in the presence of RANKL, BTO or pBTO. (C) JC-1 staining in RANKL-treated RAW264.7 cells stimulated with BTO and pBTO. (D) Schematic diagram of pBTO inhibit osteoclastogenesis of macrophage (∗: p < 0.05, ∗∗: p < 0.01, ∗∗∗∗: p < 0.001).
    Trap Staining Solution, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/trap+staining+solution/DAPI+Staining+Solution/pmc12272599-57-23-42
    Average 99 stars, based on 1 article reviews
    trap staining solution - by Bioz Stars, 2026-10
    99/100 stars
    		  Buy from Supplier

    Image Search Results


    Effect of NGF-G/P@Yoda1 on promoting bone regeneration and bone remodeling in femur def ect model. (A–D) Representative immunohistochemical staining images at week 8 for RUNX2 and OPN and semi-quantitative analysis of positive areas to evaluate the osteogenic capacity of composite hydrogels. (E, F) Representative immunofluorescence (IF) staining images at week 8 of OCN and semi-quantitative analysis. (G–J) Representative IF staining images at week 8 of YAP1and β-catenin and semi-quantitative analyses. (K, L) Representative TRAP staining images at week 8 and semi-quantitative analysis of positive areas to assess bone resorption and remodeling. The quantitative data (n ≥ 3) in the figures represent as the averages ± SD, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.

    Journal: Bioactive Materials

    Article Title: Composite hydrogel-microsphere delivery system promotes early nerve-mediated bone regeneration and late-stage mechanotransduction-driven bone remodeling via sequential release of NGF and Yoda1

    doi: 10.1016/j.bioactmat.2025.10.040

    Figure Lengend Snippet: Effect of NGF-G/P@Yoda1 on promoting bone regeneration and bone remodeling in femur def ect model. (A–D) Representative immunohistochemical staining images at week 8 for RUNX2 and OPN and semi-quantitative analysis of positive areas to evaluate the osteogenic capacity of composite hydrogels. (E, F) Representative immunofluorescence (IF) staining images at week 8 of OCN and semi-quantitative analysis. (G–J) Representative IF staining images at week 8 of YAP1and β-catenin and semi-quantitative analyses. (K, L) Representative TRAP staining images at week 8 and semi-quantitative analysis of positive areas to assess bone resorption and remodeling. The quantitative data (n ≥ 3) in the figures represent as the averages ± SD, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.

    Article Snippet: Following dewaxing and rehydration of femur sections, they were treated with TRAP staining solution (G105, Servicebio, Wuhan, China) at 37 °C for 30 min in the absence of light.

    Techniques: Immunohistochemical staining, Staining, Immunofluorescence

    pBTO inhibited osteoclastogenesis in vitro. (A) TRAP staining in RANKL-treated RAW264.7 cells stimulated with BTO and pBTO. (B) Relative mRNA levels of genes associated with osteoclastogenesis of RAW264.7 cells in the presence of RANKL, BTO or pBTO. (C) JC-1 staining in RANKL-treated RAW264.7 cells stimulated with BTO and pBTO. (D) Schematic diagram of pBTO inhibit osteoclastogenesis of macrophage (∗: p < 0.05, ∗∗: p < 0.01, ∗∗∗∗: p < 0.001).

    Journal: Materials Today Bio

    Article Title: Self-powered intracellular nanogenerator attenuates inflammatory osteolysis through mitochondrial MRS2/Mg 2+ -mediated macrophage repolarization and osteoclastogenesis inhibition

    doi: 10.1016/j.mtbio.2025.102050

    Figure Lengend Snippet: pBTO inhibited osteoclastogenesis in vitro. (A) TRAP staining in RANKL-treated RAW264.7 cells stimulated with BTO and pBTO. (B) Relative mRNA levels of genes associated with osteoclastogenesis of RAW264.7 cells in the presence of RANKL, BTO or pBTO. (C) JC-1 staining in RANKL-treated RAW264.7 cells stimulated with BTO and pBTO. (D) Schematic diagram of pBTO inhibit osteoclastogenesis of macrophage (∗: p < 0.05, ∗∗: p < 0.01, ∗∗∗∗: p < 0.001).

    Article Snippet: The cells were fixed with 4 % paraformaldehyde for 15 min, permeabilized with 0.1 % Triton X-100 for 20 min, and incubated with TRAP staining solution in the dark at 37 °C for 1 h. Then, the cells were stained with DAPI (Beyotime, China) in the dark at 37 °C for 15 min.

    Techniques: In Vitro, Staining

    pBTO improved inflammatory bone loss by modulating macrophage polarization and osteoclastogenesis in vivo. (A) Designed an mice inflammatory bone loss model. (B) Micro-CT scanning and 3D reconstruction of mice calvarial bone. pBTO inhibited LPS-induced inflammatory bone loss. (C) CD68 + Arg-1 + macrophages (arrow) in calvarial bone matrix under confocal microscope. PBTO increased the number of M2 macrophages. (D) CD68 + iNOS + macrophages (arrow) in calvarial bone matrix under confocal microscope. pBTO decreased the number of LPS-induced M1 macrophages. (E) HE staining of mice calvarial bone showed pBTO decreased LPS-induced inflammatory cell infiltration and bone resorption. (F) TRAP staining of mice calvarial bone showed pBTO decreased the number of osteoclasts.

    Journal: Materials Today Bio

    Article Title: Self-powered intracellular nanogenerator attenuates inflammatory osteolysis through mitochondrial MRS2/Mg 2+ -mediated macrophage repolarization and osteoclastogenesis inhibition

    doi: 10.1016/j.mtbio.2025.102050

    Figure Lengend Snippet: pBTO improved inflammatory bone loss by modulating macrophage polarization and osteoclastogenesis in vivo. (A) Designed an mice inflammatory bone loss model. (B) Micro-CT scanning and 3D reconstruction of mice calvarial bone. pBTO inhibited LPS-induced inflammatory bone loss. (C) CD68 + Arg-1 + macrophages (arrow) in calvarial bone matrix under confocal microscope. PBTO increased the number of M2 macrophages. (D) CD68 + iNOS + macrophages (arrow) in calvarial bone matrix under confocal microscope. pBTO decreased the number of LPS-induced M1 macrophages. (E) HE staining of mice calvarial bone showed pBTO decreased LPS-induced inflammatory cell infiltration and bone resorption. (F) TRAP staining of mice calvarial bone showed pBTO decreased the number of osteoclasts.

    Article Snippet: The cells were fixed with 4 % paraformaldehyde for 15 min, permeabilized with 0.1 % Triton X-100 for 20 min, and incubated with TRAP staining solution in the dark at 37 °C for 1 h. Then, the cells were stained with DAPI (Beyotime, China) in the dark at 37 °C for 15 min.

    Techniques: In Vivo, Micro-CT, Microscopy, Staining