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trap staining solution  (Beyotime)


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    Structured Review

    Beyotime trap staining solution
    pBTO inhibited osteoclastogenesis in vitro. (A) <t>TRAP</t> <t>staining</t> in RANKL-treated RAW264.7 cells stimulated with BTO and pBTO. (B) Relative mRNA levels of genes associated with osteoclastogenesis of RAW264.7 cells in the presence of RANKL, BTO or pBTO. (C) JC-1 staining in RANKL-treated RAW264.7 cells stimulated with BTO and pBTO. (D) Schematic diagram of pBTO inhibit osteoclastogenesis of macrophage (∗: p < 0.05, ∗∗: p < 0.01, ∗∗∗∗: p < 0.001).
    Trap Staining Solution, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 2090 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/trap+staining+solution/DAPI+Staining+Solution/pmc12272599-57-23-42
    Average 99 stars, based on 2090 article reviews
    trap staining solution - by Bioz Stars, 2026-10
    99/100 stars

    Images

    1) Product Images from "Self-powered intracellular nanogenerator attenuates inflammatory osteolysis through mitochondrial MRS2/Mg 2+ -mediated macrophage repolarization and osteoclastogenesis inhibition"

    Article Title: Self-powered intracellular nanogenerator attenuates inflammatory osteolysis through mitochondrial MRS2/Mg 2+ -mediated macrophage repolarization and osteoclastogenesis inhibition

    Journal: Materials Today Bio

    doi: 10.1016/j.mtbio.2025.102050

    pBTO inhibited osteoclastogenesis in vitro. (A) TRAP staining in RANKL-treated RAW264.7 cells stimulated with BTO and pBTO. (B) Relative mRNA levels of genes associated with osteoclastogenesis of RAW264.7 cells in the presence of RANKL, BTO or pBTO. (C) JC-1 staining in RANKL-treated RAW264.7 cells stimulated with BTO and pBTO. (D) Schematic diagram of pBTO inhibit osteoclastogenesis of macrophage (∗: p < 0.05, ∗∗: p < 0.01, ∗∗∗∗: p < 0.001).
    Figure Legend Snippet: pBTO inhibited osteoclastogenesis in vitro. (A) TRAP staining in RANKL-treated RAW264.7 cells stimulated with BTO and pBTO. (B) Relative mRNA levels of genes associated with osteoclastogenesis of RAW264.7 cells in the presence of RANKL, BTO or pBTO. (C) JC-1 staining in RANKL-treated RAW264.7 cells stimulated with BTO and pBTO. (D) Schematic diagram of pBTO inhibit osteoclastogenesis of macrophage (∗: p < 0.05, ∗∗: p < 0.01, ∗∗∗∗: p < 0.001).

    Techniques Used: In Vitro, Staining

    pBTO improved inflammatory bone loss by modulating macrophage polarization and osteoclastogenesis in vivo. (A) Designed an mice inflammatory bone loss model. (B) Micro-CT scanning and 3D reconstruction of mice calvarial bone. pBTO inhibited LPS-induced inflammatory bone loss. (C) CD68 + Arg-1 + macrophages (arrow) in calvarial bone matrix under confocal microscope. PBTO increased the number of M2 macrophages. (D) CD68 + iNOS + macrophages (arrow) in calvarial bone matrix under confocal microscope. pBTO decreased the number of LPS-induced M1 macrophages. (E) HE staining of mice calvarial bone showed pBTO decreased LPS-induced inflammatory cell infiltration and bone resorption. (F) TRAP staining of mice calvarial bone showed pBTO decreased the number of osteoclasts.
    Figure Legend Snippet: pBTO improved inflammatory bone loss by modulating macrophage polarization and osteoclastogenesis in vivo. (A) Designed an mice inflammatory bone loss model. (B) Micro-CT scanning and 3D reconstruction of mice calvarial bone. pBTO inhibited LPS-induced inflammatory bone loss. (C) CD68 + Arg-1 + macrophages (arrow) in calvarial bone matrix under confocal microscope. PBTO increased the number of M2 macrophages. (D) CD68 + iNOS + macrophages (arrow) in calvarial bone matrix under confocal microscope. pBTO decreased the number of LPS-induced M1 macrophages. (E) HE staining of mice calvarial bone showed pBTO decreased LPS-induced inflammatory cell infiltration and bone resorption. (F) TRAP staining of mice calvarial bone showed pBTO decreased the number of osteoclasts.

    Techniques Used: In Vivo, Micro-CT, Microscopy, Staining

    Related Articles

    Labeling:

    Article Title: TRPM7 function in ischemia-reperfusion renal injury and its regulatory mechanism of PARthanatos
    Article Snippet: .. Upon completion of all labeling procedures, DAPI staining solution (C1005, Beyotime, Shanghai, China) was added for 5 min of nuclear staining in the dark, and residual DAPI was washed off with PBS. .. Slides were treated with tissue autofluorescence quencher for 5 min in darkness and rinsed under running tap water for 10 min, then mounted with anti-fluorescence quenching mounting medium (P0126, Beyotime, Shanghai, China).

    Staining:

    Article Title: TRPM7 function in ischemia-reperfusion renal injury and its regulatory mechanism of PARthanatos
    Article Snippet: .. Upon completion of all labeling procedures, DAPI staining solution (C1005, Beyotime, Shanghai, China) was added for 5 min of nuclear staining in the dark, and residual DAPI was washed off with PBS. .. Slides were treated with tissue autofluorescence quencher for 5 min in darkness and rinsed under running tap water for 10 min, then mounted with anti-fluorescence quenching mounting medium (P0126, Beyotime, Shanghai, China).

    Article Title: Establishment and validation of porcine embryo genomic selection system through SCNT technique.
    Article Snippet: After ten washes, embryos were placed into diluted secondary antibodies (anti-mouse IgG 488, Abbkine, A23210, 1:500; anti-rabbit IgG 549, Abbkine, A23320, 1:500) for 1 h at room temperature, followed by another ten washes. .. Finally, embryos were incubated in DAPI staining solution for 3 min and washed ten times before mounting in a 2-μL drop of mounting medium (Beyotime, P0126) on a glass slide. ..

    Article Title: Establishment and validation of porcine embryo genomic selection system through SCNT technique.
    Article Snippet: .. The nuclei of TE biopsy cells were then dyed by 4’,6-diamidino-2-phenylindole (DAPI) staining solution (Beyotime, P0131), and captured using an inverted microscope equipped with fluorescence illumination system (Nikon, Eclipse Ti2-U). ..

    Article Title: Tectorigenin reprograms macrophages through targeting OXCT1-mediated ketone metabolism to suppress IFN-I signaling and ameliorate myocardial ischemia-reperfusion injury
    Article Snippet: .. The sections were rinsed with PBS for another 3 cycles, 5 min per wash. 4’,6-diamidino-2-phenylindole (DAPI) staining solution (Cat. No.: C1005; Beyotime Jo urn al Pr e-p roo f 7 / 40 Biotechnology) was added dropwise and incubated at room temperature in the dark for 5 min for nuclear counterstaining. ..

    Article Title: Dexmedetomidine Activates AMPK/SIRT1 Pathway to Reduce Inflammatory Levels and Neuronal Pyroptosis to Alleviate Neonatal Hypoxic-Ischemic Brain Damage.
    Article Snippet: Neonatal hypoxic-ischemic brain damage (HIBD) is the leading cause of neonatal death and long-term neurological dysfunction.. Dexmedetomidine (DEX) has been shown to have neuroprotective effects, but its mechanisms in HIBD remain incompletely understood.. This study aimed to explore whether DEX inhibits neuroinflammation and pyroptosis via activating adenosine monophosphate-activated protein kinase (AMPK)/sirtuin 1 (SIRT1) axis, thereby reducing HIBD.

    Article Title: Hierarchically sequential targeting nanoplatform suppresses NETosis-promoted angiogenesis for corneal neovascularization therapy.
    Article Snippet: The Phorbol 12-myristate 13-acetate (PMA, S1819) was purchased from Beyotime (Shanghai, China). .. The 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride Jo ur na l P re -p ro of (DAPI, C1005) Staining Solution was purchased from Beyotime (Shanghai, China). .. The clamidine hydrochloride was purchased from Sparkjade (SJ-MX0367A, Shandong, China).

    Article Title: Butyrate counteracts maternal obesity-induced cardiac defects by promoting PLEKHG3-actin binding
    Article Snippet: Vector TrueVIEW kit , Vector laboratories , SP-8400. .. DAPI Staining Solution , Beyotime , C1006. .. mTeSR1 medium , STEMCELL , 85850.

    Incubation:

    Article Title: Establishment and validation of porcine embryo genomic selection system through SCNT technique.
    Article Snippet: After ten washes, embryos were placed into diluted secondary antibodies (anti-mouse IgG 488, Abbkine, A23210, 1:500; anti-rabbit IgG 549, Abbkine, A23320, 1:500) for 1 h at room temperature, followed by another ten washes. .. Finally, embryos were incubated in DAPI staining solution for 3 min and washed ten times before mounting in a 2-μL drop of mounting medium (Beyotime, P0126) on a glass slide. ..

    Article Title: Tectorigenin reprograms macrophages through targeting OXCT1-mediated ketone metabolism to suppress IFN-I signaling and ameliorate myocardial ischemia-reperfusion injury
    Article Snippet: .. The sections were rinsed with PBS for another 3 cycles, 5 min per wash. 4’,6-diamidino-2-phenylindole (DAPI) staining solution (Cat. No.: C1005; Beyotime Jo urn al Pr e-p roo f 7 / 40 Biotechnology) was added dropwise and incubated at room temperature in the dark for 5 min for nuclear counterstaining. ..

    Article Title: Dexmedetomidine Activates AMPK/SIRT1 Pathway to Reduce Inflammatory Levels and Neuronal Pyroptosis to Alleviate Neonatal Hypoxic-Ischemic Brain Damage.
    Article Snippet: Neonatal hypoxic-ischemic brain damage (HIBD) is the leading cause of neonatal death and long-term neurological dysfunction.. Dexmedetomidine (DEX) has been shown to have neuroprotective effects, but its mechanisms in HIBD remain incompletely understood.. This study aimed to explore whether DEX inhibits neuroinflammation and pyroptosis via activating adenosine monophosphate-activated protein kinase (AMPK)/sirtuin 1 (SIRT1) axis, thereby reducing HIBD.

    Glass:

    Article Title: Establishment and validation of porcine embryo genomic selection system through SCNT technique.
    Article Snippet: After ten washes, embryos were placed into diluted secondary antibodies (anti-mouse IgG 488, Abbkine, A23210, 1:500; anti-rabbit IgG 549, Abbkine, A23320, 1:500) for 1 h at room temperature, followed by another ten washes. .. Finally, embryos were incubated in DAPI staining solution for 3 min and washed ten times before mounting in a 2-μL drop of mounting medium (Beyotime, P0126) on a glass slide. ..

    Inverted Microscopy:

    Article Title: Establishment and validation of porcine embryo genomic selection system through SCNT technique.
    Article Snippet: .. The nuclei of TE biopsy cells were then dyed by 4’,6-diamidino-2-phenylindole (DAPI) staining solution (Beyotime, P0131), and captured using an inverted microscope equipped with fluorescence illumination system (Nikon, Eclipse Ti2-U). ..

    Fluorescence:

    Article Title: Establishment and validation of porcine embryo genomic selection system through SCNT technique.
    Article Snippet: .. The nuclei of TE biopsy cells were then dyed by 4’,6-diamidino-2-phenylindole (DAPI) staining solution (Beyotime, P0131), and captured using an inverted microscope equipped with fluorescence illumination system (Nikon, Eclipse Ti2-U). ..

    other:

    Article Title: Lacticaseibacillus rhamnosus GG enhances gefitinib efficacy and reduces treatment-related toxicity via propionyl-L-carnitine regulation.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Propionyl-L-carnitine (PLC) Aladdin Biochemical Technology, Shanghai, China CAS: 20064-19-1 Acetonitrile Xilong Scientific Co., Ltd., China CAS: 75-05-8 Formic acid Xilong Scientific Co., Ltd., China CAS: 64-18-6 Methotrexate Yuanye Bio-Technology, Shanghai, China CAS: 59-05-2 Luciferin sodium salt Yuanye Bio-Technology, Shanghai, China Cat#: B25437 Sodium carboxymethylcellulose (CMC-Na) Solarbio Life Sciences, Beijing, China CAS: 9004-32-4 Universal Tissue Fixative (Neutral) Servicebio, Wuhan, China Cat#: G1101 Trypsin Gibco Cat#: 25200-056 Fetal bovine serum Gibco Cat#: A5669701 Penicillin-streptomycin Gibco Cat#: 15140122 Dulbecco’s Modified Eagle Medium (DMEM) Gibco Cat#: 11965092 RPMI 1640 medium Gibco Cat#: C11875500BT BSA blocking solution Thermo Fisher Scientific Cat#: 37525 RIPA lysis buffer Thermo Fisher Scientific Cat#: 89901 RIPA buffer Beyotime Biotechnology Cat#: P0013B PMSF Beyotime Biotechnology Cat#: ST507 Protease/phosphatase inhibitor cocktail Beyotime Biotechnology Cat#: P1045 0.1% Triton X-100 Beyotime Biotechnology Cat#: P0096 DAPI staining solution Beyotime Biotechnology Cat#: C1006 Anhydrous Sodium Sulfate Xilong Scientific Co., Ltd., China CAS: 7757-82-6 Sodium Chloride (NaCl) Xilong Scientific Co., Ltd., China CAS: 7647-14-5 Dilute Sulfuric Acid (H2SO4) Xilong Scientific Co., Ltd., China CAS: 7664-93-9 n-Hexane Xilong Scientific Co., Ltd., China CAS: 110-54-3 Collagenase Type IV Sigma-Aldrich Cat#: C4-28 DNase I Beyotime Biotechnology Cat#: D7073 Critical commercial assays CCK-8 Kit MedChemExpress Cat#: HY-K0301 MTT Cell Proliferation and Cytotoxicity Assay Kit Beyotime Biotechnology Cat#: C0009S Annexin V-FITC/PI Apoptosis Detection Kit Huiyucheng Biotechnology, Wuhan, China Cat#: HYC2019 BCA Protein Assay Kit Thermo Fisher Scientific Cat#: 23225 TUNEL Assay Kit Beyotime Biotechnology Cat#: C1098 DAB Substrate Kit Beyotime Biotechnology Cat# P0202 Pierce ECL Western Blotting Substrate Thermo Fisher Scientific Cat#: 32106 CEA ELISA Kit Xin Yu Biotech, Shanghai, China Cat#: XY9M0916 CYFRA21-1 ELISA Kit Xin Yu Biotech, Shanghai, China Cat#: XY926701a GLUT1 ELISA Kit Xin Yu Biotech, Shanghai, China Cat#: XY9NM11861 LDHA ELISA Kit Xin Yu Biotech, Shanghai, China Cat#: XY9NM1898 Mitochondrial Complex I Activity Assay Kit Xin Yu Biotech, Shanghai, China Cat#: XY9737601a Mitochondrial Complex II Activity Assay Kit Xin Yu Biotech, Shanghai, China Cat#: XY9737701a Mitochondrial Complex III Activity Assay Kit Xin Yu Biotech, Shanghai, China Cat#: XY9737801a Mitochondrial Complex IV Activity Assay Kit Xin Yu Biotech, Shanghai, China Cat#: XY9737901a Mitochondrial Complex V Activity Assay Kit Xin Yu Biotech, Shanghai, China Cat#: XY9737011a Propionyl-L-carnitine (PLC) ELISA Kit Xin Yu Biotech, Shanghai, China Cat#: XY972791a Mouse ROS ELISA Kit Xin Yu Biotech, Shanghai, China Cat#: XYM9437001 Mouse IKKα ELISA Kit Xin Yu Biotech, Shanghai, China Cat#: XY9438821a (Continued on next page) 18 Cell Reports 45, 118068, October 27, 2026



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    Image Search Results


    Effect of NGF-G/P@Yoda1 on promoting bone regeneration and bone remodeling in femur def ect model. (A–D) Representative immunohistochemical staining images at week 8 for RUNX2 and OPN and semi-quantitative analysis of positive areas to evaluate the osteogenic capacity of composite hydrogels. (E, F) Representative immunofluorescence (IF) staining images at week 8 of OCN and semi-quantitative analysis. (G–J) Representative IF staining images at week 8 of YAP1and β-catenin and semi-quantitative analyses. (K, L) Representative TRAP staining images at week 8 and semi-quantitative analysis of positive areas to assess bone resorption and remodeling. The quantitative data (n ≥ 3) in the figures represent as the averages ± SD, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.

    Journal: Bioactive Materials

    Article Title: Composite hydrogel-microsphere delivery system promotes early nerve-mediated bone regeneration and late-stage mechanotransduction-driven bone remodeling via sequential release of NGF and Yoda1

    doi: 10.1016/j.bioactmat.2025.10.040

    Figure Lengend Snippet: Effect of NGF-G/P@Yoda1 on promoting bone regeneration and bone remodeling in femur def ect model. (A–D) Representative immunohistochemical staining images at week 8 for RUNX2 and OPN and semi-quantitative analysis of positive areas to evaluate the osteogenic capacity of composite hydrogels. (E, F) Representative immunofluorescence (IF) staining images at week 8 of OCN and semi-quantitative analysis. (G–J) Representative IF staining images at week 8 of YAP1and β-catenin and semi-quantitative analyses. (K, L) Representative TRAP staining images at week 8 and semi-quantitative analysis of positive areas to assess bone resorption and remodeling. The quantitative data (n ≥ 3) in the figures represent as the averages ± SD, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.

    Article Snippet: Following dewaxing and rehydration of femur sections, they were treated with TRAP staining solution (G105, Servicebio, Wuhan, China) at 37 °C for 30 min in the absence of light.

    Techniques: Immunohistochemical staining, Staining, Immunofluorescence