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Servicebio Inc tris glycine transfer buffer
Tris Glycine Transfer Buffer, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/transfer+buffer/buffer+rapid+transfer/pm42273917-60-7-21
Average 86 stars, based on 1 article reviews
tris glycine transfer buffer - by Bioz Stars, 2026-09
86/100 stars

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Related Articles

Electrophoresis:

Article Title: Thymol Mitigates Oxidative Stress-Induced Ovarian Aging and Restores Steroidogenesis via the JAK1–STAT3 Pathway
Article Snippet: Total protein concentration was determined using a bicinchoninic acid protein assay (P0010S, Beyotime, Shanghai, China), and samples were then diluted to 4 μg/mL. .. Protein samples were separated using SWE rapid high-resolution electrophoresis buffer (G2081-1L, Servicebio, Wuhan, China) and transferred to a 0.45 μm polyvinylidene difluoride membrane (G6047-0.45, Servicebio, Wuhan, China). ..

Article Title: pH-Responsive Resveratrol Liposomes Targeting Sigma1 Receptor Attenuate Renal Fibrosis by Remodeling the Renal Microenvironment.
Article Snippet: Renal fibrosis (RF), a critical pathogenic mechanism in chronic kidney disease (CKD), now has exceedingly few therapeutic alternatives.. This study presents a new sigma1 receptor (σ1R)-targeted nanodelivery system utilizing the natural polyphenolic medicine resveratrol (Res) as a therapeutic agent, addressing its low bioavailability and inadequate targeting properties, thereby providing a significant advancement in the treatment of RF.. We engineered and produced a multifunctional material, aminoethyl anisamide-poly(2-ethyl-2-oxazoline)-cholesterol hemisuccinate (APC)-with benefits including active targeting, prolonged circulation, and pH sensitivity.

Article Title: Thymol Mitigates Oxidative Stress-Induced Ovarian Aging and Restores Steroidogenesis via the JAK1–STAT3 Pathway
Article Snippet: Total protein concentration was determined using a bicinchoninic acid protein assay (P0010S, Beyotime, Shanghai, China), and samples were then diluted to 4 μg/mL. .. Protein samples were separated using SWE rapid high-resolution electrophoresis buffer (G2081-1L, Servicebio, Wuhan, China) and transferred to a 0.45 μm polyvinylidene difluoride membrane (G6047-0.45, Servicebio, Wuhan, China). ..

Article Title: Enhancing small-molecule-mediated translational control through multivalent RNA aptamers.
Article Snippet: PVDF membranes were purchased from Millipore. .. Electrophoresis and transfer supplies, including Tris-buffered saline, Tris-glycine transfer buffer, SWE Rapid High-Resolution Electrophoresis Buffer, and filter papers, were sourced from Servicebio. .. Primary antibodies used included β-Actin Mouse Monoclonal Antibody (Beyotime Biotechnology), BAX Polyclonal Antibody, and E-cadherin Polyclonal Antibody (Proteintech).

Article Title: Carbon Dots Derived from Ligusticum chuanxiong Alleviate Acute Kidney Injury by Scavenging ROS and Restoring Mitochondrial Homeostasis
Article Snippet: Enzyme-linked immunosorbent assay (ELISA) kits for interleukin-6 (IL-6; ER0042), interleukin-1β (IL-1β, ER1094), and tumor necrosis factor-alpha (TNF-α; ER1393) were obtained from FineTest (Wuhan, China). .. SWE fast-HR electrophoresis buffer (G2081), ice-free rapid transfer buffer (G2028), and electron microscopy fixative solution (G1102) were obtained from Servicebio (China).Catalase (CAT) assay kit (S0051) and total superoxide dismutase (SOD) assay kit (S0101) were obtained from Beyotime (China). ..

Article Title: Lactylation Converts ABHD6 into a Mitochondrial Regulator that Drives Lenvatinib Resistance in Hepatocellular Carcinoma
Article Snippet: 6 Affiliations 7 1 Department of Pharmacy, Affiliated Hospital of Nantong University, Pharmacy School of 8 Nantong University, Nantong, China 9 2 Department of oncology, School of Medicine, Nantong University, Nantong, China 10 3 Department of Pharmacy, Rugao People′s Hospital, Rugao, Jiangsu,China 11 4 Department of General Surgery, Affiliated Hospital of Nantong University, Nantong 12 University, China 13 5 State Key Laboratory of Quality Research in Chinese Medicine, School of Pharmacy, Macau 14 University of Science and Technology, Macau SAR 999078, China 15 6 Key Laboratory of Drug Metabolism and Pharmacokinetics, State Key Laboratory of Natural 16 Medicines, China Pharmaceutical University, Nanjing, China 17 18

Membrane:

Article Title: Thymol Mitigates Oxidative Stress-Induced Ovarian Aging and Restores Steroidogenesis via the JAK1–STAT3 Pathway
Article Snippet: Total protein concentration was determined using a bicinchoninic acid protein assay (P0010S, Beyotime, Shanghai, China), and samples were then diluted to 4 μg/mL. .. Protein samples were separated using SWE rapid high-resolution electrophoresis buffer (G2081-1L, Servicebio, Wuhan, China) and transferred to a 0.45 μm polyvinylidene difluoride membrane (G6047-0.45, Servicebio, Wuhan, China). ..

Article Title: Thymol Mitigates Oxidative Stress-Induced Ovarian Aging and Restores Steroidogenesis via the JAK1–STAT3 Pathway
Article Snippet: Total protein concentration was determined using a bicinchoninic acid protein assay (P0010S, Beyotime, Shanghai, China), and samples were then diluted to 4 μg/mL. .. Protein samples were separated using SWE rapid high-resolution electrophoresis buffer (G2081-1L, Servicebio, Wuhan, China) and transferred to a 0.45 μm polyvinylidene difluoride membrane (G6047-0.45, Servicebio, Wuhan, China). ..

Staining:

Article Title: pH-Responsive Resveratrol Liposomes Targeting Sigma1 Receptor Attenuate Renal Fibrosis by Remodeling the Renal Microenvironment.
Article Snippet: Renal fibrosis (RF), a critical pathogenic mechanism in chronic kidney disease (CKD), now has exceedingly few therapeutic alternatives.. This study presents a new sigma1 receptor (σ1R)-targeted nanodelivery system utilizing the natural polyphenolic medicine resveratrol (Res) as a therapeutic agent, addressing its low bioavailability and inadequate targeting properties, thereby providing a significant advancement in the treatment of RF.. We engineered and produced a multifunctional material, aminoethyl anisamide-poly(2-ethyl-2-oxazoline)-cholesterol hemisuccinate (APC)-with benefits including active targeting, prolonged circulation, and pH sensitivity.

Polyacrylamide Gel Electrophoresis:

Article Title: pH-Responsive Resveratrol Liposomes Targeting Sigma1 Receptor Attenuate Renal Fibrosis by Remodeling the Renal Microenvironment.
Article Snippet: Renal fibrosis (RF), a critical pathogenic mechanism in chronic kidney disease (CKD), now has exceedingly few therapeutic alternatives.. This study presents a new sigma1 receptor (σ1R)-targeted nanodelivery system utilizing the natural polyphenolic medicine resveratrol (Res) as a therapeutic agent, addressing its low bioavailability and inadequate targeting properties, thereby providing a significant advancement in the treatment of RF.. We engineered and produced a multifunctional material, aminoethyl anisamide-poly(2-ethyl-2-oxazoline)-cholesterol hemisuccinate (APC)-with benefits including active targeting, prolonged circulation, and pH sensitivity.

Article Title: Lactylation Converts ABHD6 into a Mitochondrial Regulator that Drives Lenvatinib Resistance in Hepatocellular Carcinoma
Article Snippet: 6 Affiliations 7 1 Department of Pharmacy, Affiliated Hospital of Nantong University, Pharmacy School of 8 Nantong University, Nantong, China 9 2 Department of oncology, School of Medicine, Nantong University, Nantong, China 10 3 Department of Pharmacy, Rugao People′s Hospital, Rugao, Jiangsu,China 11 4 Department of General Surgery, Affiliated Hospital of Nantong University, Nantong 12 University, China 13 5 State Key Laboratory of Quality Research in Chinese Medicine, School of Pharmacy, Macau 14 University of Science and Technology, Macau SAR 999078, China 15 6 Key Laboratory of Drug Metabolism and Pharmacokinetics, State Key Laboratory of Natural 16 Medicines, China Pharmaceutical University, Nanjing, China 17 18

other:

Article Title: Acetylation of CPT1A reduces fatty acid oxidation and leads to dysfunctional renal tubular mitochondria in diabetic kidney disease.
Article Snippet: We used the following reagents and materials: bicinchoninic acid assay kit (Wandai Bio; WLA004a), blood glucose meter (Sannuo; GA-3), biochemical indicators (Mindray BS-240 Vet), anti-CPT1A rabbit monoclonal antibody (Cell Signaling Technology; #97361), anti–β-actin rabbit polyclonal antibody (Servicebio; GB11001-100), anti–β-actin mouse monoclonal antibody (Servicebio; GB12001-100), anti-glyceraldehyde 3-phosphate dehydrogenase antibodies (Servicebio; GB15002100 and GB15004-100), anti–β-tubulin mouse monoclonal antibody (Servicebio; GB122667-100), anti–goat antirabbit immunoglobulin (Ig) G horseradish peroxide–conjugated antibody (EARTHOX; E030120-01), goat antimouse IgG horseradish peroxide–conjugated antibody (EARTHOX; E030110-01), antifibronectin mouse monoclonal antibody (Bioswamp; 1E2), anti–α-smooth muscle actin mouse monoclonal antibody (Bioswamp; 3G2), anti–acetyl-lysine rabbit monoclonal antibody (Hangzhou Jingjie Biotech; PTM0105RM), Chekine fatty acid synthase (FAS) activity detection kit (Abbkine; KTB2240), mitochondrial membrane potential kit (Solarbio; M8650), reactive oxygen species (ROS) detection kit (Beyotime; SOO33S), two-color prestained protein marker (Shanghai Yazy; WJ102), electrochemiluminescent substrate (Biosharp; BL520A), 5X sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis (SDS-PAGE) protein loading buffer (Beyotime; P0015), immunoprecipitation kit for protein A + G (Beyotime; P2179S), fetal bovine serum (Procell; 164210), LV-CPT1A lentivirus (Genechem Co. Ltd.; LVCON335), trypsin–ethylenediaminetetraacetate digestion solution (Bio-Channel; BC-CE-005), protein-free rapid blocking buffer (Shanghai Yaji Biomedical Technology Co., Ltd.; PS108P), acetylase inhibitor (Beyotime; P1112), primary antibody diluent for Western blotting (Beyotime; P0023A), omni-easy one-step PAGE gel fast preparation kit (Shanghai Yazy; PG210, PG212, and PG213), SWE fast highresolution electrophoresis buffer (Servicebio; G2081), ice-free rapid transfer buffer (Servicebio; G2028), Dulbecco’s modified Eagle medium (DMEM), low-glucose (Servicebio; G4520), DMEM/F12 (Servicebio; G4610), and polyvinylidene fluoride membranes (Millipore; IPVH00010).

Saline:

Article Title: Enhancing small-molecule-mediated translational control through multivalent RNA aptamers.
Article Snippet: PVDF membranes were purchased from Millipore. .. Electrophoresis and transfer supplies, including Tris-buffered saline, Tris-glycine transfer buffer, SWE Rapid High-Resolution Electrophoresis Buffer, and filter papers, were sourced from Servicebio. .. Primary antibodies used included β-Actin Mouse Monoclonal Antibody (Beyotime Biotechnology), BAX Polyclonal Antibody, and E-cadherin Polyclonal Antibody (Proteintech).

Electron Microscopy:

Article Title: Carbon Dots Derived from Ligusticum chuanxiong Alleviate Acute Kidney Injury by Scavenging ROS and Restoring Mitochondrial Homeostasis
Article Snippet: Enzyme-linked immunosorbent assay (ELISA) kits for interleukin-6 (IL-6; ER0042), interleukin-1β (IL-1β, ER1094), and tumor necrosis factor-alpha (TNF-α; ER1393) were obtained from FineTest (Wuhan, China). .. SWE fast-HR electrophoresis buffer (G2081), ice-free rapid transfer buffer (G2028), and electron microscopy fixative solution (G1102) were obtained from Servicebio (China).Catalase (CAT) assay kit (S0051) and total superoxide dismutase (SOD) assay kit (S0101) were obtained from Beyotime (China). ..

Chloramphenicol Acetyltransferase Assay:

Article Title: Carbon Dots Derived from Ligusticum chuanxiong Alleviate Acute Kidney Injury by Scavenging ROS and Restoring Mitochondrial Homeostasis
Article Snippet: Enzyme-linked immunosorbent assay (ELISA) kits for interleukin-6 (IL-6; ER0042), interleukin-1β (IL-1β, ER1094), and tumor necrosis factor-alpha (TNF-α; ER1393) were obtained from FineTest (Wuhan, China). .. SWE fast-HR electrophoresis buffer (G2081), ice-free rapid transfer buffer (G2028), and electron microscopy fixative solution (G1102) were obtained from Servicebio (China).Catalase (CAT) assay kit (S0051) and total superoxide dismutase (SOD) assay kit (S0101) were obtained from Beyotime (China). ..

Functional Assay:

Article Title: Lactylation Converts ABHD6 into a Mitochondrial Regulator that Drives Lenvatinib Resistance in Hepatocellular Carcinoma
Article Snippet: 6 Affiliations 7 1 Department of Pharmacy, Affiliated Hospital of Nantong University, Pharmacy School of 8 Nantong University, Nantong, China 9 2 Department of oncology, School of Medicine, Nantong University, Nantong, China 10 3 Department of Pharmacy, Rugao People′s Hospital, Rugao, Jiangsu,China 11 4 Department of General Surgery, Affiliated Hospital of Nantong University, Nantong 12 University, China 13 5 State Key Laboratory of Quality Research in Chinese Medicine, School of Pharmacy, Macau 14 University of Science and Technology, Macau SAR 999078, China 15 6 Key Laboratory of Drug Metabolism and Pharmacokinetics, State Key Laboratory of Natural 16 Medicines, China Pharmaceutical University, Nanjing, China 17 18

Protein Extraction:

Article Title: Lactylation Converts ABHD6 into a Mitochondrial Regulator that Drives Lenvatinib Resistance in Hepatocellular Carcinoma
Article Snippet: 6 Affiliations 7 1 Department of Pharmacy, Affiliated Hospital of Nantong University, Pharmacy School of 8 Nantong University, Nantong, China 9 2 Department of oncology, School of Medicine, Nantong University, Nantong, China 10 3 Department of Pharmacy, Rugao People′s Hospital, Rugao, Jiangsu,China 11 4 Department of General Surgery, Affiliated Hospital of Nantong University, Nantong 12 University, China 13 5 State Key Laboratory of Quality Research in Chinese Medicine, School of Pharmacy, Macau 14 University of Science and Technology, Macau SAR 999078, China 15 6 Key Laboratory of Drug Metabolism and Pharmacokinetics, State Key Laboratory of Natural 16 Medicines, China Pharmaceutical University, Nanjing, China 17 18

Lysis:

Article Title: Lactylation Converts ABHD6 into a Mitochondrial Regulator that Drives Lenvatinib Resistance in Hepatocellular Carcinoma
Article Snippet: 6 Affiliations 7 1 Department of Pharmacy, Affiliated Hospital of Nantong University, Pharmacy School of 8 Nantong University, Nantong, China 9 2 Department of oncology, School of Medicine, Nantong University, Nantong, China 10 3 Department of Pharmacy, Rugao People′s Hospital, Rugao, Jiangsu,China 11 4 Department of General Surgery, Affiliated Hospital of Nantong University, Nantong 12 University, China 13 5 State Key Laboratory of Quality Research in Chinese Medicine, School of Pharmacy, Macau 14 University of Science and Technology, Macau SAR 999078, China 15 6 Key Laboratory of Drug Metabolism and Pharmacokinetics, State Key Laboratory of Natural 16 Medicines, China Pharmaceutical University, Nanjing, China 17 18

Protease Inhibitor:

Article Title: Lactylation Converts ABHD6 into a Mitochondrial Regulator that Drives Lenvatinib Resistance in Hepatocellular Carcinoma
Article Snippet: 6 Affiliations 7 1 Department of Pharmacy, Affiliated Hospital of Nantong University, Pharmacy School of 8 Nantong University, Nantong, China 9 2 Department of oncology, School of Medicine, Nantong University, Nantong, China 10 3 Department of Pharmacy, Rugao People′s Hospital, Rugao, Jiangsu,China 11 4 Department of General Surgery, Affiliated Hospital of Nantong University, Nantong 12 University, China 13 5 State Key Laboratory of Quality Research in Chinese Medicine, School of Pharmacy, Macau 14 University of Science and Technology, Macau SAR 999078, China 15 6 Key Laboratory of Drug Metabolism and Pharmacokinetics, State Key Laboratory of Natural 16 Medicines, China Pharmaceutical University, Nanjing, China 17 18

Marker:

Article Title: Lactylation Converts ABHD6 into a Mitochondrial Regulator that Drives Lenvatinib Resistance in Hepatocellular Carcinoma
Article Snippet: 6 Affiliations 7 1 Department of Pharmacy, Affiliated Hospital of Nantong University, Pharmacy School of 8 Nantong University, Nantong, China 9 2 Department of oncology, School of Medicine, Nantong University, Nantong, China 10 3 Department of Pharmacy, Rugao People′s Hospital, Rugao, Jiangsu,China 11 4 Department of General Surgery, Affiliated Hospital of Nantong University, Nantong 12 University, China 13 5 State Key Laboratory of Quality Research in Chinese Medicine, School of Pharmacy, Macau 14 University of Science and Technology, Macau SAR 999078, China 15 6 Key Laboratory of Drug Metabolism and Pharmacokinetics, State Key Laboratory of Natural 16 Medicines, China Pharmaceutical University, Nanjing, China 17 18

Bicinchoninic Acid Protein Assay:

Article Title: Lactylation Converts ABHD6 into a Mitochondrial Regulator that Drives Lenvatinib Resistance in Hepatocellular Carcinoma
Article Snippet: 6 Affiliations 7 1 Department of Pharmacy, Affiliated Hospital of Nantong University, Pharmacy School of 8 Nantong University, Nantong, China 9 2 Department of oncology, School of Medicine, Nantong University, Nantong, China 10 3 Department of Pharmacy, Rugao People′s Hospital, Rugao, Jiangsu,China 11 4 Department of General Surgery, Affiliated Hospital of Nantong University, Nantong 12 University, China 13 5 State Key Laboratory of Quality Research in Chinese Medicine, School of Pharmacy, Macau 14 University of Science and Technology, Macau SAR 999078, China 15 6 Key Laboratory of Drug Metabolism and Pharmacokinetics, State Key Laboratory of Natural 16 Medicines, China Pharmaceutical University, Nanjing, China 17 18



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