other:
Saline:Article Title: Dietary goat milk extracellular vesicles remodel the microbiota–gut–brain axis to alleviate adolescent anxiety
Article Snippet: Safe and effective strategies for managing adolescent anxiety disorders are urgently needed.. This study demonstrates that goat milk-derived extracellular vesicles (gEVs), a natural nanostructure from dairy origin, effectively alleviate chronic restraint stress (CRS)-induced anxiety-like behaviors in adolescent mice through modulation of the gut–brain axis.. The purified gEVs were characterized by transmission electron microscopy, nanoparticle tracking analysis, and western blotting, confirming their nanoscale morphology and vesicular protein markers.
Electrophoresis:Article Title: Dietary goat milk extracellular vesicles remodel the microbiota–gut–brain axis to alleviate adolescent anxiety
Article Snippet: Safe and effective strategies for managing adolescent anxiety disorders are urgently needed.. This study demonstrates that goat milk-derived extracellular vesicles (gEVs), a natural nanostructure from dairy origin, effectively alleviate chronic restraint stress (CRS)-induced anxiety-like behaviors in adolescent mice through modulation of the gut–brain axis.. The purified gEVs were characterized by transmission electron microscopy, nanoparticle tracking analysis, and western blotting, confirming their nanoscale morphology and vesicular protein markers.
Membrane:
SDS Page:Article Title: Thioredoxin system dysregulation and calpain activation drive myocardial disulfidptosis via pathological disulfide bonds remodeling.
Article Snippet: .. SDS-PAGE Gel Quick Preparation Kit(Epizyme Biotech,PG111/PG112/PG113), Omni-ECLTM Enhanced Pico Light Chemiluminescence Kit(Epizyme Biotech,SQ101), Protein Sample Loading Buffer (Denaturing, non-Reducing) (Epizyme Biotech,LT103), Omni-EasyTMProtein Sample Loading Buffer(Denaturing,Reducing) (Epizyme Biotech,LT101),Tris-Glycine-SDS(Epizyme Biotech,TF101),Tris-Glycine(Epizyme Biotech,TF102), Multicolor Prestained Protein Ladder(Epizyme Biotech,WJ103), Cell Counting Kit-8(MeilunBio,MA0218), Tris(2-carboxyethyl)phosphine hydrochloride (MedChemExpress,HY-W011500), Diethylmaleate(Yeasen, 53857ES60), Auranofin (MedChemExpress, HY-B1123)NADPH(Beyotime,ST360), Nifedipine(MCE, HY-B0284), S107 (MCE, HY-15292), Bay K 8644 (MCE, HY-10588), MDL-28170 (MCE, HY-18236),NADP+/NADPH Assay Kit AR TI CL E IN P RE SS with WST-8(Beyotime,S0179), Cysteine(Cys) Content Assay Kit(Solarbio, BC0180), Actin-Tracker Red555(Beyotime, C2203S), Triton X-100(Beyotime, P0096), DAPI Staining Solution(Beyotime, C1005), PVDF(Millipore,0.45 μm, IPVH00010), HRP-conjugated Affinipure Goat Anti-Mouse IgG(H+L)( Proteintech, SA00001-1), Actin Monoclonal Antibody (ACTN05 (C4))( Thermo, MA511869), Thioredoxin Polyclonal antibody(Proteintech, 14999-1-AP), FLNA Monoclonal antibody(Proteintech, 67133-1-Ig), Alpha Tubulin Polyclonal antibody(Proteintech, 11224-1-AP), Calpain 1 Polyclonal antibody(Proteintech, 10538-1-AP), Multi-rAbTM CoraLite® Plus 488-Goat AntiRabbit Recombinant Secondary Antibody (H+L) (Proteintech, RGAR002), Beta Actin Monoclonal antibody(Proteintech, 66009-1-Ig)Propidium lodide(PI) (Yeasen, 40710ES03), Glucose-free DMEM(Pricella, PM150270), Glucose-and cystine-free DMEM(customized from Pricella), DMEM(Pricella,PM150210), Fetal Bovine Serum(Pricella, 164210),2,3, 5-triphenyltetrazolium chloride(TTC)(Sigma, T8877), Oxidized Thioredoxin Reductase(TrxR) Activity Assay Kit(Solarbio, BC1150), Fluo-4 AM(Beyotime, S1060), Calcium Colorimetric Assay Kit(Beyotime, S1063). .. Cell culture Rat cardiomyocytes (H9C2) were purchased from Wuhan Pricella Biotechnology.
Cell Counting:Article Title: Thioredoxin system dysregulation and calpain activation drive myocardial disulfidptosis via pathological disulfide bonds remodeling.
Article Snippet: .. SDS-PAGE Gel Quick Preparation Kit(Epizyme Biotech,PG111/PG112/PG113), Omni-ECLTM Enhanced Pico Light Chemiluminescence Kit(Epizyme Biotech,SQ101), Protein Sample Loading Buffer (Denaturing, non-Reducing) (Epizyme Biotech,LT103), Omni-EasyTMProtein Sample Loading Buffer(Denaturing,Reducing) (Epizyme Biotech,LT101),Tris-Glycine-SDS(Epizyme Biotech,TF101),Tris-Glycine(Epizyme Biotech,TF102), Multicolor Prestained Protein Ladder(Epizyme Biotech,WJ103), Cell Counting Kit-8(MeilunBio,MA0218), Tris(2-carboxyethyl)phosphine hydrochloride (MedChemExpress,HY-W011500), Diethylmaleate(Yeasen, 53857ES60), Auranofin (MedChemExpress, HY-B1123)NADPH(Beyotime,ST360), Nifedipine(MCE, HY-B0284), S107 (MCE, HY-15292), Bay K 8644 (MCE, HY-10588), MDL-28170 (MCE, HY-18236),NADP+/NADPH Assay Kit AR TI CL E IN P RE SS with WST-8(Beyotime,S0179), Cysteine(Cys) Content Assay Kit(Solarbio, BC0180), Actin-Tracker Red555(Beyotime, C2203S), Triton X-100(Beyotime, P0096), DAPI Staining Solution(Beyotime, C1005), PVDF(Millipore,0.45 μm, IPVH00010), HRP-conjugated Affinipure Goat Anti-Mouse IgG(H+L)( Proteintech, SA00001-1), Actin Monoclonal Antibody (ACTN05 (C4))( Thermo, MA511869), Thioredoxin Polyclonal antibody(Proteintech, 14999-1-AP), FLNA Monoclonal antibody(Proteintech, 67133-1-Ig), Alpha Tubulin Polyclonal antibody(Proteintech, 11224-1-AP), Calpain 1 Polyclonal antibody(Proteintech, 10538-1-AP), Multi-rAbTM CoraLite® Plus 488-Goat AntiRabbit Recombinant Secondary Antibody (H+L) (Proteintech, RGAR002), Beta Actin Monoclonal antibody(Proteintech, 66009-1-Ig)Propidium lodide(PI) (Yeasen, 40710ES03), Glucose-free DMEM(Pricella, PM150270), Glucose-and cystine-free DMEM(customized from Pricella), DMEM(Pricella,PM150210), Fetal Bovine Serum(Pricella, 164210),2,3, 5-triphenyltetrazolium chloride(TTC)(Sigma, T8877), Oxidized Thioredoxin Reductase(TrxR) Activity Assay Kit(Solarbio, BC1150), Fluo-4 AM(Beyotime, S1060), Calcium Colorimetric Assay Kit(Beyotime, S1063). .. Cell culture Rat cardiomyocytes (H9C2) were purchased from Wuhan Pricella Biotechnology.
Staining:Article Title: Thioredoxin system dysregulation and calpain activation drive myocardial disulfidptosis via pathological disulfide bonds remodeling.
Article Snippet: .. SDS-PAGE Gel Quick Preparation Kit(Epizyme Biotech,PG111/PG112/PG113), Omni-ECLTM Enhanced Pico Light Chemiluminescence Kit(Epizyme Biotech,SQ101), Protein Sample Loading Buffer (Denaturing, non-Reducing) (Epizyme Biotech,LT103), Omni-EasyTMProtein Sample Loading Buffer(Denaturing,Reducing) (Epizyme Biotech,LT101),Tris-Glycine-SDS(Epizyme Biotech,TF101),Tris-Glycine(Epizyme Biotech,TF102), Multicolor Prestained Protein Ladder(Epizyme Biotech,WJ103), Cell Counting Kit-8(MeilunBio,MA0218), Tris(2-carboxyethyl)phosphine hydrochloride (MedChemExpress,HY-W011500), Diethylmaleate(Yeasen, 53857ES60), Auranofin (MedChemExpress, HY-B1123)NADPH(Beyotime,ST360), Nifedipine(MCE, HY-B0284), S107 (MCE, HY-15292), Bay K 8644 (MCE, HY-10588), MDL-28170 (MCE, HY-18236),NADP+/NADPH Assay Kit AR TI CL E IN P RE SS with WST-8(Beyotime,S0179), Cysteine(Cys) Content Assay Kit(Solarbio, BC0180), Actin-Tracker Red555(Beyotime, C2203S), Triton X-100(Beyotime, P0096), DAPI Staining Solution(Beyotime, C1005), PVDF(Millipore,0.45 μm, IPVH00010), HRP-conjugated Affinipure Goat Anti-Mouse IgG(H+L)( Proteintech, SA00001-1), Actin Monoclonal Antibody (ACTN05 (C4))( Thermo, MA511869), Thioredoxin Polyclonal antibody(Proteintech, 14999-1-AP), FLNA Monoclonal antibody(Proteintech, 67133-1-Ig), Alpha Tubulin Polyclonal antibody(Proteintech, 11224-1-AP), Calpain 1 Polyclonal antibody(Proteintech, 10538-1-AP), Multi-rAbTM CoraLite® Plus 488-Goat AntiRabbit Recombinant Secondary Antibody (H+L) (Proteintech, RGAR002), Beta Actin Monoclonal antibody(Proteintech, 66009-1-Ig)Propidium lodide(PI) (Yeasen, 40710ES03), Glucose-free DMEM(Pricella, PM150270), Glucose-and cystine-free DMEM(customized from Pricella), DMEM(Pricella,PM150210), Fetal Bovine Serum(Pricella, 164210),2,3, 5-triphenyltetrazolium chloride(TTC)(Sigma, T8877), Oxidized Thioredoxin Reductase(TrxR) Activity Assay Kit(Solarbio, BC1150), Fluo-4 AM(Beyotime, S1060), Calcium Colorimetric Assay Kit(Beyotime, S1063). .. Cell culture Rat cardiomyocytes (H9C2) were purchased from Wuhan Pricella Biotechnology.
Recombinant:Article Title: Thioredoxin system dysregulation and calpain activation drive myocardial disulfidptosis via pathological disulfide bonds remodeling.
Article Snippet: .. SDS-PAGE Gel Quick Preparation Kit(Epizyme Biotech,PG111/PG112/PG113), Omni-ECLTM Enhanced Pico Light Chemiluminescence Kit(Epizyme Biotech,SQ101), Protein Sample Loading Buffer (Denaturing, non-Reducing) (Epizyme Biotech,LT103), Omni-EasyTMProtein Sample Loading Buffer(Denaturing,Reducing) (Epizyme Biotech,LT101),Tris-Glycine-SDS(Epizyme Biotech,TF101),Tris-Glycine(Epizyme Biotech,TF102), Multicolor Prestained Protein Ladder(Epizyme Biotech,WJ103), Cell Counting Kit-8(MeilunBio,MA0218), Tris(2-carboxyethyl)phosphine hydrochloride (MedChemExpress,HY-W011500), Diethylmaleate(Yeasen, 53857ES60), Auranofin (MedChemExpress, HY-B1123)NADPH(Beyotime,ST360), Nifedipine(MCE, HY-B0284), S107 (MCE, HY-15292), Bay K 8644 (MCE, HY-10588), MDL-28170 (MCE, HY-18236),NADP+/NADPH Assay Kit AR TI CL E IN P RE SS with WST-8(Beyotime,S0179), Cysteine(Cys) Content Assay Kit(Solarbio, BC0180), Actin-Tracker Red555(Beyotime, C2203S), Triton X-100(Beyotime, P0096), DAPI Staining Solution(Beyotime, C1005), PVDF(Millipore,0.45 μm, IPVH00010), HRP-conjugated Affinipure Goat Anti-Mouse IgG(H+L)( Proteintech, SA00001-1), Actin Monoclonal Antibody (ACTN05 (C4))( Thermo, MA511869), Thioredoxin Polyclonal antibody(Proteintech, 14999-1-AP), FLNA Monoclonal antibody(Proteintech, 67133-1-Ig), Alpha Tubulin Polyclonal antibody(Proteintech, 11224-1-AP), Calpain 1 Polyclonal antibody(Proteintech, 10538-1-AP), Multi-rAbTM CoraLite® Plus 488-Goat AntiRabbit Recombinant Secondary Antibody (H+L) (Proteintech, RGAR002), Beta Actin Monoclonal antibody(Proteintech, 66009-1-Ig)Propidium lodide(PI) (Yeasen, 40710ES03), Glucose-free DMEM(Pricella, PM150270), Glucose-and cystine-free DMEM(customized from Pricella), DMEM(Pricella,PM150210), Fetal Bovine Serum(Pricella, 164210),2,3, 5-triphenyltetrazolium chloride(TTC)(Sigma, T8877), Oxidized Thioredoxin Reductase(TrxR) Activity Assay Kit(Solarbio, BC1150), Fluo-4 AM(Beyotime, S1060), Calcium Colorimetric Assay Kit(Beyotime, S1063). .. Cell culture Rat cardiomyocytes (H9C2) were purchased from Wuhan Pricella Biotechnology.
Activity Assay:Article Title: Thioredoxin system dysregulation and calpain activation drive myocardial disulfidptosis via pathological disulfide bonds remodeling.
Article Snippet: .. SDS-PAGE Gel Quick Preparation Kit(Epizyme Biotech,PG111/PG112/PG113), Omni-ECLTM Enhanced Pico Light Chemiluminescence Kit(Epizyme Biotech,SQ101), Protein Sample Loading Buffer (Denaturing, non-Reducing) (Epizyme Biotech,LT103), Omni-EasyTMProtein Sample Loading Buffer(Denaturing,Reducing) (Epizyme Biotech,LT101),Tris-Glycine-SDS(Epizyme Biotech,TF101),Tris-Glycine(Epizyme Biotech,TF102), Multicolor Prestained Protein Ladder(Epizyme Biotech,WJ103), Cell Counting Kit-8(MeilunBio,MA0218), Tris(2-carboxyethyl)phosphine hydrochloride (MedChemExpress,HY-W011500), Diethylmaleate(Yeasen, 53857ES60), Auranofin (MedChemExpress, HY-B1123)NADPH(Beyotime,ST360), Nifedipine(MCE, HY-B0284), S107 (MCE, HY-15292), Bay K 8644 (MCE, HY-10588), MDL-28170 (MCE, HY-18236),NADP+/NADPH Assay Kit AR TI CL E IN P RE SS with WST-8(Beyotime,S0179), Cysteine(Cys) Content Assay Kit(Solarbio, BC0180), Actin-Tracker Red555(Beyotime, C2203S), Triton X-100(Beyotime, P0096), DAPI Staining Solution(Beyotime, C1005), PVDF(Millipore,0.45 μm, IPVH00010), HRP-conjugated Affinipure Goat Anti-Mouse IgG(H+L)( Proteintech, SA00001-1), Actin Monoclonal Antibody (ACTN05 (C4))( Thermo, MA511869), Thioredoxin Polyclonal antibody(Proteintech, 14999-1-AP), FLNA Monoclonal antibody(Proteintech, 67133-1-Ig), Alpha Tubulin Polyclonal antibody(Proteintech, 11224-1-AP), Calpain 1 Polyclonal antibody(Proteintech, 10538-1-AP), Multi-rAbTM CoraLite® Plus 488-Goat AntiRabbit Recombinant Secondary Antibody (H+L) (Proteintech, RGAR002), Beta Actin Monoclonal antibody(Proteintech, 66009-1-Ig)Propidium lodide(PI) (Yeasen, 40710ES03), Glucose-free DMEM(Pricella, PM150270), Glucose-and cystine-free DMEM(customized from Pricella), DMEM(Pricella,PM150210), Fetal Bovine Serum(Pricella, 164210),2,3, 5-triphenyltetrazolium chloride(TTC)(Sigma, T8877), Oxidized Thioredoxin Reductase(TrxR) Activity Assay Kit(Solarbio, BC1150), Fluo-4 AM(Beyotime, S1060), Calcium Colorimetric Assay Kit(Beyotime, S1063). .. Cell culture Rat cardiomyocytes (H9C2) were purchased from Wuhan Pricella Biotechnology.
Calcium Colorimetric Assay:Article Title: Thioredoxin system dysregulation and calpain activation drive myocardial disulfidptosis via pathological disulfide bonds remodeling.
Article Snippet: .. SDS-PAGE Gel Quick Preparation Kit(Epizyme Biotech,PG111/PG112/PG113), Omni-ECLTM Enhanced Pico Light Chemiluminescence Kit(Epizyme Biotech,SQ101), Protein Sample Loading Buffer (Denaturing, non-Reducing) (Epizyme Biotech,LT103), Omni-EasyTMProtein Sample Loading Buffer(Denaturing,Reducing) (Epizyme Biotech,LT101),Tris-Glycine-SDS(Epizyme Biotech,TF101),Tris-Glycine(Epizyme Biotech,TF102), Multicolor Prestained Protein Ladder(Epizyme Biotech,WJ103), Cell Counting Kit-8(MeilunBio,MA0218), Tris(2-carboxyethyl)phosphine hydrochloride (MedChemExpress,HY-W011500), Diethylmaleate(Yeasen, 53857ES60), Auranofin (MedChemExpress, HY-B1123)NADPH(Beyotime,ST360), Nifedipine(MCE, HY-B0284), S107 (MCE, HY-15292), Bay K 8644 (MCE, HY-10588), MDL-28170 (MCE, HY-18236),NADP+/NADPH Assay Kit AR TI CL E IN P RE SS with WST-8(Beyotime,S0179), Cysteine(Cys) Content Assay Kit(Solarbio, BC0180), Actin-Tracker Red555(Beyotime, C2203S), Triton X-100(Beyotime, P0096), DAPI Staining Solution(Beyotime, C1005), PVDF(Millipore,0.45 μm, IPVH00010), HRP-conjugated Affinipure Goat Anti-Mouse IgG(H+L)( Proteintech, SA00001-1), Actin Monoclonal Antibody (ACTN05 (C4))( Thermo, MA511869), Thioredoxin Polyclonal antibody(Proteintech, 14999-1-AP), FLNA Monoclonal antibody(Proteintech, 67133-1-Ig), Alpha Tubulin Polyclonal antibody(Proteintech, 11224-1-AP), Calpain 1 Polyclonal antibody(Proteintech, 10538-1-AP), Multi-rAbTM CoraLite® Plus 488-Goat AntiRabbit Recombinant Secondary Antibody (H+L) (Proteintech, RGAR002), Beta Actin Monoclonal antibody(Proteintech, 66009-1-Ig)Propidium lodide(PI) (Yeasen, 40710ES03), Glucose-free DMEM(Pricella, PM150270), Glucose-and cystine-free DMEM(customized from Pricella), DMEM(Pricella,PM150210), Fetal Bovine Serum(Pricella, 164210),2,3, 5-triphenyltetrazolium chloride(TTC)(Sigma, T8877), Oxidized Thioredoxin Reductase(TrxR) Activity Assay Kit(Solarbio, BC1150), Fluo-4 AM(Beyotime, S1060), Calcium Colorimetric Assay Kit(Beyotime, S1063). .. Cell culture Rat cardiomyocytes (H9C2) were purchased from Wuhan Pricella Biotechnology.
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